##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780726_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38676 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.623177164132795 34.0 31.0 34.0 12.0 34.0 2 31.02140862550419 34.0 32.0 34.0 23.0 34.0 3 31.113869066087496 34.0 33.0 34.0 23.0 34.0 4 31.523373668424863 34.0 34.0 34.0 23.0 34.0 5 31.464060399213984 34.0 34.0 34.0 23.0 34.0 6 34.12589202606267 38.0 34.0 38.0 23.0 38.0 7 33.731151101458266 37.0 34.0 38.0 21.0 38.0 8 33.05346985210466 37.0 34.0 38.0 21.0 38.0 9 33.52606267452684 37.0 34.0 38.0 21.0 38.0 10-11 33.82499482883442 37.5 34.0 38.0 22.0 38.0 12-13 34.091051297962565 38.0 34.5 38.0 22.0 38.0 14-15 33.793308511738545 38.0 34.0 38.0 21.0 38.0 16-17 33.60227272727273 37.5 34.0 38.0 21.0 38.0 18-19 33.61253749095046 37.5 34.0 38.0 21.0 38.0 20-21 33.69782293929052 38.0 34.0 38.0 21.0 38.0 22-23 33.632071568931636 37.5 34.0 38.0 21.0 38.0 24-25 34.05683110973213 38.0 34.5 38.0 22.0 38.0 26-27 34.067160512979626 38.0 34.5 38.0 22.0 38.0 28-29 34.21243148205606 38.0 35.0 38.0 23.0 38.0 30-31 34.06800082738649 38.0 35.0 38.0 22.0 38.0 32-33 33.7374728513807 38.0 34.0 38.0 21.0 38.0 34-35 33.77855776191954 37.0 34.0 38.0 21.0 38.0 36-37 33.75519702140863 37.5 34.0 38.0 21.0 38.0 38-39 34.18091322784156 38.0 35.0 38.0 23.0 38.0 40-41 33.70534698521047 37.5 34.0 38.0 21.0 38.0 42-43 33.97339435308719 38.0 34.0 38.0 21.5 38.0 44-45 33.11777329610094 37.0 34.0 38.0 16.0 38.0 46-47 33.78180266832144 37.0 34.0 38.0 21.0 38.0 48-49 33.59277071051815 37.0 34.0 38.0 21.0 38.0 50-51 33.457415451442756 37.0 34.0 38.0 21.0 38.0 52-53 33.485572448029785 37.0 34.0 38.0 21.0 38.0 54-55 33.242954286896264 37.0 34.0 38.0 21.0 38.0 56-57 33.29563553624987 37.0 33.5 38.0 20.5 38.0 58-59 33.185554348950255 37.0 33.0 38.0 21.0 38.0 60-61 33.23010394042817 37.0 33.5 38.0 20.5 38.0 62-63 33.242178612059156 37.0 33.5 38.0 20.0 38.0 64-65 33.276166097838455 37.0 33.0 38.0 20.0 38.0 66-67 33.21521098355569 37.0 33.5 38.0 20.0 38.0 68-69 32.79672148102182 37.0 31.5 38.0 15.0 38.0 70-71 32.730052228772365 37.0 32.0 38.0 19.0 38.0 72-73 32.851393629124004 37.0 32.0 38.0 19.0 38.0 74-75 32.38938876822836 37.0 31.0 38.0 15.0 38.0 76 31.70938049436343 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.3239466792024963 1101 2 0.47020563102487856 1101 3 0.25109348702827816 1101 4 -1.0458251513914867 1101 5 -0.7717129776507186 1101 6 -2.5483431059226653 1101 7 -0.5275208728079548 1101 8 -0.42560529221427856 1101 9 0.022957634521524994 1101 10-11 -0.05208439329116743 1101 12-13 -1.1190562694355464 1101 14-15 -0.7063221111656404 1101 16-17 -1.2374158975524878 1101 18-19 0.39944727211450015 1101 20-21 -0.25416135955564556 1101 22-23 0.14988163846289382 1101 24-25 -1.7068814929571943 1101 26-27 -0.7477827416108198 1101 28-29 -0.7570515011137218 1101 30-31 -0.7202347738964221 1101 32-33 0.4060023156698236 1101 34-35 -0.6824458039925858 1101 36-37 0.1445987061850218 1101 38-39 -1.3807221485384105 1101 40-41 -0.008977836068162048 1101 42-43 0.23516120934077378 1101 44-45 1.4487582495254259 1101 46-47 0.8454642719064509 1101 48-49 -1.063415418039007 1101 50-51 -0.4192388087361536 1101 52-53 0.01646475987503493 1101 54-55 -0.8780194157297103 1101 56-57 -0.35444837430248555 1101 58-59 -0.014682081118529311 1101 60-61 -0.38989900041681125 1101 62-63 -1.637188970954604 1101 64-65 -0.8403898152775469 1101 66-67 -0.20638088691436707 1101 68-69 0.5548535216687327 1101 70-71 1.2159906874138997 1101 72-73 -0.7729343306366303 1101 74-75 -0.7828472587071502 1101 76 -1.299066166091965 1102 1 -0.17012129666424158 1102 2 -0.704861875725797 1102 3 -0.7760892312435494 1102 4 -0.40514108298464535 1102 5 -0.6504708534382964 1102 6 -0.20486875848791897 1102 7 0.54007588686801 1102 8 0.5366809364085867 1102 9 -0.41961662290422197 1102 10-11 -1.5335875436061954 1102 12-13 -3.976662030011603 1102 14-15 -1.7132528042349477 1102 16-17 -3.234805636526385 1102 18-19 -1.9420018727999917 1102 20-21 -0.3076717105907463 1102 22-23 -0.915329882689214 1102 24-25 -0.4580516099688978 1102 26-27 -6.4550284661832755 1102 28-29 -0.9974655425178582 1102 30-31 -0.4282005704760792 1102 32-33 -7.147913075869326 1102 34-35 -1.8159291523274206 1102 36-37 0.11741598791319774 1102 38-39 0.06032195587203404 1102 40-41 0.2744155032657716 1102 42-43 -0.22843014979514464 1102 44-45 -5.931889815281053 1102 46-47 -0.42123239776051946 1102 48-49 -0.43132720921812506 1102 50-51 -6.510372922147496 1102 52-53 -2.0370905956605156 1102 54-55 -0.10196180996912574 1102 56-57 -1.789596889153973 1102 58-59 0.10921530862044193 1102 60-61 -0.8890889194087137 1102 62-63 -5.4516804200995175 1102 64-65 -1.1066814444404685 1102 66-67 -0.3724924980754807 1102 68-69 -7.686235587242159 1102 70-71 -1.2786537770325452 1102 72-73 -1.214248462049774 1102 74-75 -6.423621336114888 1102 76 -1.7460508645618091 1103 1 0.43648320994026335 1103 2 -1.0199356907996133 1103 3 -0.6281482833026004 1103 4 -0.17250705035061387 1103 5 0.16312666015921806 1103 6 -1.6580156116347737 1103 7 -0.12868868189655558 1103 8 0.4467819465095957 1103 9 -0.4204713237589175 1103 10-11 0.17826171824306414 1103 12-13 -1.2205625739121473 1103 14-15 -1.1371844281665702 1103 16-17 -1.3764528781736232 1103 18-19 -1.132794413592535 1103 20-21 -1.4252707781898195 1103 22-23 -1.1086865260458652 1103 24-25 -0.22545642737371452 1103 26-27 -1.0030082641630713 1103 28-29 -0.3535649986173155 1103 30-31 -0.42870562098112686 1103 32-33 -5.428177256133509 1103 34-35 -1.6472422836405514 1103 36-37 -0.5081473376501293 1103 38-39 -0.12266172711164813 1103 40-41 -0.14784556899529377 1103 42-43 -0.09762206898706438 1103 44-45 -5.237795021186258 1103 46-47 -0.8201445966727121 1103 48-49 -0.7139612418521608 1103 50-51 -0.4732711350457137 1103 52-53 -0.6466477052176316 1103 54-55 -0.3544093624166891 1103 56-57 0.4937364441793548 1103 58-59 0.13209798150311514 1103 60-61 0.4405148101950189 1103 62-63 -0.104904973324075 1103 64-65 0.11006292230389647 1103 66-67 -0.5101381857211749 1103 68-69 -3.9174321684387365 1103 70-71 -0.30402285240161575 1103 72-73 0.1708331230318123 1103 74-75 -1.5119663244598769 1103 76 -1.189562908073853 1104 1 -1.2611844707782645 1104 2 -0.5207324458336551 1104 3 -0.09997212800780275 1104 4 0.16234736077498368 1104 5 -0.30963880413166933 1104 6 -0.7825266937729722 1104 7 1.1866090147570745 1104 8 1.3912263909540385 1104 9 0.47529863133306804 1104 10-11 -0.9503055713411754 1104 12-13 -0.26757112092069235 1104 14-15 0.1604760093243769 1104 16-17 -0.9564543268191414 1104 18-19 0.6965189284326812 1104 20-21 1.1534993217667235 1104 22-23 0.2342078676959929 1104 24-25 0.11968336691862191 1104 26-27 0.03842299760717083 1104 28-29 -0.3277428922867358 1104 30-31 -0.46024987710165277 1104 32-33 -0.8946773285657841 1104 34-35 -0.44196921396070366 1104 36-37 0.5435330911489373 1104 38-39 0.3977641746701863 1104 40-41 0.8177128838512857 1104 42-43 0.7757300967379877 1104 44-45 -1.9117049154351378 1104 46-47 0.05288162381113182 1104 48-49 0.25626909278495447 1104 50-51 1.0685947203794655 1104 52-53 0.4640650283764671 1104 54-55 0.013908760138725995 1104 56-57 -0.11078332983193917 1104 58-59 0.586719160700568 1104 60-61 0.08610368459745388 1104 62-63 0.44793437188815943 1104 64-65 -0.05175078188268856 1104 66-67 0.6682625096286685 1104 68-69 -3.348947451648936 1104 70-71 -0.24868459367352358 1104 72-73 0.09075924211047237 1104 74-75 -1.0340219524477092 1104 76 0.24532047596207107 1105 1 -0.8331850046098381 1105 2 -0.3265512100135588 1105 3 0.1848270178616751 1105 4 0.198581179005334 1105 5 0.9201089604486015 1105 6 0.8622751212543847 1105 7 1.131557633467871 1105 8 1.2455570996154535 1105 9 0.5244778652847586 1105 10-11 1.2238569803737605 1105 12-13 -0.7282010421805367 1105 14-15 -0.09592996958927813 1105 16-17 1.0573919296869292 1105 18-19 0.7261799453818298 1105 20-21 0.5743254261307911 1105 22-23 0.526080281949298 1105 24-25 0.7411967794369758 1105 26-27 0.02761290819037754 1105 28-29 -0.4522257429473555 1105 30-31 0.6404107394738219 1105 32-33 0.6704834881965311 1105 34-35 0.9726177338572626 1105 36-37 0.6767574338688078 1105 38-39 0.24428759653059018 1105 40-41 1.1266345440674925 1105 42-43 0.7925791558598334 1105 44-45 1.9258768275249594 1105 46-47 1.19301241254729 1105 48-49 0.5330607650123653 1105 50-51 -0.025226179126740078 1105 52-53 -0.03530104662686995 1105 54-55 0.13862516211390385 1105 56-57 0.2115842132082335 1105 58-59 -0.1477997235914401 1105 60-61 0.7381122259026753 1105 62-63 0.2010039034509461 1105 64-65 0.4688017337986139 1105 66-67 1.36791551910418 1105 68-69 1.91573291669485 1105 70-71 1.6865215987727495 1105 72-73 0.615815961715434 1105 74-75 -0.14996349789011987 1105 76 -0.3142684737243009 1106 1 -0.5337576603006049 1106 2 0.5405926697287491 1106 3 -1.539725594879915 1106 4 0.24940437156081074 1106 5 0.09498369201624968 1106 6 -0.22305057666974193 1106 7 0.6400758868680114 1106 8 1.2821354818631292 1106 9 1.5349288316412384 1106 10-11 0.27095791093925925 1106 12-13 0.24151978817021558 1106 14-15 -0.5223437133258528 1106 16-17 0.8697398180190703 1106 18-19 -0.4783655091636234 1106 20-21 0.0923282894092452 1106 22-23 0.6255792082198752 1106 24-25 0.6601302082129195 1106 26-27 -0.3095739207287309 1106 28-29 -0.8156473606996784 1106 30-31 -0.5782005704760778 1106 32-33 1.042996015039762 1106 34-35 0.7886163022180384 1106 36-37 0.7719614424586538 1106 38-39 0.43304922859930883 1106 40-41 0.4971427759930407 1106 42-43 1.1079334865684913 1106 44-45 1.0408374574462158 1106 46-47 0.9105857840576661 1106 48-49 0.045945518054601564 1106 50-51 0.9032634414888676 1106 52-53 0.49927304070311607 1106 54-55 1.2253109173036023 1106 56-57 1.4058576563005758 1106 58-59 0.05921530862044477 1106 60-61 0.8290928987731121 1106 62-63 0.8392286708095682 1106 64-65 0.8751367373777157 1106 66-67 0.8184165928336 1106 68-69 1.2955825945760182 1106 70-71 0.43498258660381595 1106 72-73 0.8130242652229569 1106 74-75 -1.4736213361148884 1106 76 0.3812218627109196 1107 1 -0.46681551154027545 1107 2 -0.8825478261390209 1107 3 -1.228981793227021 1107 4 -2.24646339703423 1107 5 -1.3422063906283803 1107 6 -0.8709844609672572 1107 7 -0.4028993197435611 1107 8 -0.8029884850790197 1107 9 -1.6617653832348012 1107 10-11 -1.0216040725318152 1107 12-13 -0.6353397159620187 1107 14-15 -0.7859800769622183 1107 16-17 -1.3124915869396077 1107 18-19 -0.19117542651899555 1107 20-21 -1.2998204709213255 1107 22-23 -1.5562389735983047 1107 24-25 -1.5320185521176555 1107 26-27 -1.5190780529601398 1107 28-29 -2.033829178881497 1107 30-31 -2.3658038762612 1107 32-33 -0.7561775386792391 1107 34-35 -0.7762597308398185 1107 36-37 -1.8561377310950675 1107 38-39 -1.2371987052849889 1107 40-41 -0.1127745793788506 1107 42-43 -1.137107835745553 1107 44-45 -1.7508980797438625 1107 46-47 -1.398091901892741 1107 48-49 -0.892071010871021 1107 50-51 -1.1281415171888156 1107 52-53 -0.8470079510324169 1107 54-55 -0.8391518926137564 1107 56-57 -1.0751340792366193 1107 58-59 -1.6069003938588935 1107 60-61 -1.7477666053591214 1107 62-63 -1.8781267010912543 1107 64-65 -1.2236235932007986 1107 66-67 -1.3472858865052402 1107 68-69 0.035665239204121235 1107 70-71 -1.2534471654622976 1107 72-73 -0.7944137513059744 1107 74-75 -0.9781667906603424 1107 76 -1.4931583025783404 1108 1 -2.1679641317952125 1108 2 -0.33429176786755477 1108 3 -0.879633144956955 1108 4 -1.4478221307504384 1108 5 -1.1909947363350604 1108 6 -0.2486283886882319 1108 7 0.22898189611300523 1108 8 0.3403789333269174 1108 9 -0.524701368666932 1108 10-11 -0.6536954018496459 1108 12-13 -0.7506219683783186 1108 14-15 -0.44545619406545 1108 16-17 -0.8420475471581241 1108 18-19 -0.6890742919063086 1108 20-21 -0.8380261019620932 1108 22-23 -2.134436200100616 1108 24-25 -1.736248836471205 1108 26-27 -1.3090346295114728 1108 28-29 -0.4929971295748743 1108 30-31 -0.8585549618474175 1108 32-33 -0.7929824133115488 1108 34-35 -0.8741726037912159 1108 36-37 -0.49460250207139467 1108 38-39 -0.46240531685523933 1108 40-41 -1.0085583025893925 1108 42-43 -0.16494786936371497 1108 44-45 -1.0769591527232691 1108 46-47 -0.264914986358356 1108 48-49 -0.5318665004353846 1108 50-51 -0.6220832457222301 1108 52-53 -0.7986468360303149 1108 54-55 -1.2445658161324573 1108 56-57 -0.24637655017092897 1108 58-59 -1.4726028731977365 1108 60-61 -0.6469471628601724 1108 62-63 -2.2851164755694704 1108 64-65 -1.1831067140860796 1108 66-67 -0.9037713886764109 1108 68-69 0.24427288733411956 1108 70-71 0.5225018470044418 1108 72-73 -0.324494995177659 1108 74-75 0.016825505179411948 1108 76 -0.8478998630209773 1109 1 -1.0250552515981965 1109 2 -1.2677989386628603 1109 3 0.004330349176029813 1109 4 -0.6340455118890738 1109 5 0.11184460887716696 1109 6 0.18813823451907297 1109 7 -1.0432185964264704 1109 8 0.4724229721506177 1109 9 1.1265372232496276 1109 10-11 0.6654412054225531 1109 12-13 0.02730067395110325 1109 14-15 -0.8294921204742636 1109 16-17 -1.1285896303103797 1109 18-19 -0.15416193496005093 1109 20-21 0.6217377688187256 1109 22-23 0.6306297132703733 1109 24-25 0.5565948546775701 1109 26-27 0.5995558384010309 1109 28-29 0.6464350013826845 1109 30-31 0.6268499345744303 1109 32-33 1.1786603507040994 1109 34-35 -0.01903715543542006 1109 36-37 0.5559552264524328 1109 38-39 1.1465690421191255 1109 40-41 0.7581373369876019 1109 42-43 0.14169417032917409 1109 44-45 0.15109386770262745 1109 46-47 -0.6577514342795467 1109 48-49 -0.4831920110829273 1109 50-51 0.9968143350397582 1109 52-53 0.3405317819618574 1109 54-55 -0.40141790942523414 1109 56-57 0.49800994845286795 1109 58-59 -0.2824319330267997 1109 60-61 0.09863446831467826 1109 62-63 -0.617725486144586 1109 64-65 -0.018142205901227726 1109 66-67 -0.6041552797382721 1109 68-69 0.7107729597663877 1109 70-71 0.12760107922231612 1109 72-73 -0.4744660222673289 1109 74-75 1.6247858122922594 1109 76 -0.924605643116589 1110 1 -0.21286451835483078 1110 2 -0.5964089251675837 1110 3 -0.961256695358383 1110 4 -0.7561777656002739 1110 5 -0.6582858454638156 1110 6 -0.8327794442933438 1110 7 0.18170268112638865 1110 8 1.531577268147018 1110 9 -2.3161078509743938 1110 10-11 -1.9181170491883393 1110 12-13 -1.000027261271569 1110 14-15 -0.2939545586209107 1110 16-17 -0.6104834674673754 1110 18-19 -0.401013037074307 1110 20-21 -1.5909252991074965 1110 22-23 -0.6559199943319598 1110 24-25 -0.3893115780709735 1110 26-27 -1.6228913050987472 1110 28-29 -0.44252136388946894 1110 30-31 -0.7956646853086085 1110 32-33 0.4458668284368912 1110 34-35 0.0793659034939509 1110 36-37 -0.29143568672795794 1110 38-39 0.37092801647808926 1110 40-41 -0.6900183085364588 1110 42-43 0.19477559183165027 1110 44-45 0.6004865802532393 1110 46-47 -0.403927772561147 1110 48-49 0.39602526287119133 1110 50-51 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0.8713680250724707 2116 48-49 1.139497315094772 2116 50-51 0.5948067818694156 2116 52-53 1.7430362542337718 2116 54-55 -0.2417081101805536 2116 56-57 -0.30471316822374206 2116 58-59 -0.571440082500061 2116 60-61 -1.7404631265968682 2116 62-63 0.5545563663698303 2116 64-65 1.10906908410076 2116 66-67 0.09346944695622739 2116 68-69 0.40213650578110105 2116 70-71 -1.152015299231273 2116 72-73 0.9779291278022342 2116 74-75 -1.0389490316751413 2116 76 -1.8500677778810513 2117 1 1.7822851204480585 2117 2 1.4507530975362322 2117 3 1.3789909826601914 2117 4 1.0461958154110746 2117 5 1.0944489326579614 2117 6 -0.2914997745306991 2117 7 -0.9588545944154134 2117 8 -0.6852441972812571 2117 9 1.1627363182722519 2117 10-11 0.9720274296558387 2117 12-13 0.9383112320204816 2117 14-15 0.5338060192944667 2117 16-17 0.7892585345966125 2117 18-19 1.0320622983230052 2117 20-21 1.3637186637407979 2117 22-23 0.9999107590220149 2117 24-25 0.6951569461808376 2117 26-27 1.4270570953140478 2117 28-29 0.5993259013324064 2117 30-31 0.6255427450319502 2117 32-33 1.8601083145049984 2117 34-35 1.1338034679934381 2117 36-37 0.3195550253463537 2117 38-39 1.440535859615352 2117 40-41 -0.07077166250962819 2117 42-43 0.3049923100979015 2117 44-45 1.4820139280344478 2117 46-47 -0.3501094031081067 2117 48-49 0.3496888335626238 2117 50-51 1.3786645110075781 2117 52-53 1.4703960353555203 2117 54-55 0.30712909912178077 2117 56-57 0.21334428731660893 2117 58-59 0.053867715037561936 2117 60-61 2.0317666955645493 2117 62-63 0.5124907028951355 2117 64-65 0.9807517106397512 2117 66-67 -0.5000326050273571 2117 68-69 0.5755291186401976 2117 70-71 1.7023622657482065 2117 72-73 1.5868210566667997 2117 74-75 1.3501754553289658 2117 76 0.1737352316948737 2118 1 0.8799895680807701 2118 2 0.39824233713451207 2118 3 0.733223407337384 2118 4 0.4622646819820986 2118 5 -1.0012469066533747 2118 6 0.5887010863014339 2118 7 -0.02112145237810381 2118 8 -4.413651657826449 2118 9 -0.7074215009529965 2118 10-11 -1.199995525867834 2118 12-13 0.5129166839573571 2118 14-15 -0.6778869505764078 2118 16-17 -1.6458921110274929 2118 18-19 -1.2982102985206083 2118 20-21 -3.069423373561925 2118 22-23 -1.061782210848861 2118 24-25 -0.5294485057560365 2118 26-27 -0.0348510825912598 2118 28-29 -0.2943613296575478 2118 30-31 -0.7423912578374967 2118 32-33 0.3235946846628224 2118 34-35 0.1298579873621577 2118 36-37 -1.0379055198473353 2118 38-39 -1.1758199510015785 2118 40-41 -2.3460944745612764 2118 42-43 -1.3225543183095567 2118 44-45 -0.027455225480608192 2118 46-47 -0.8030505795786951 2118 48-49 -1.6414159010141347 2118 50-51 -1.2770026338991585 2118 52-53 -1.4788866045296984 2118 54-55 0.12542178204860477 2118 56-57 1.0628421352362736 2118 58-59 0.2956898097290903 2118 60-61 -2.2031687420251167 2118 62-63 0.014284103182077956 2118 64-65 -0.6181005131766071 2118 66-67 -2.294221988097661 2118 68-69 0.4198619737334468 2118 70-71 -1.030205883462699 2118 72-73 -0.9973970208080871 2118 74-75 0.12327445102479828 2118 76 -1.9342992015906368 2119 1 0.8655430390000944 2119 2 0.6860472151832937 2119 3 0.9581765030221874 2119 4 -0.2904557682993314 2119 5 0.48449418152674184 2119 6 1.6804459268267635 2119 7 -2.1235604767683505 2119 8 0.4758417755694211 2119 9 -1.8734627767503724 2119 10-11 0.2765523165336603 2119 12-13 0.6170442636946945 2119 14-15 0.6021318111496683 2119 16-17 0.5449146431938914 2119 18-19 0.3877184069202926 2119 20-21 0.04652409360505061 2119 22-23 1.1451596278002896 2119 24-25 0.17796237604508747 2119 26-27 1.0935729324181267 2119 28-29 0.3549820099296923 2119 30-31 0.3875336952581847 2119 32-33 -0.02133964929589638 2119 34-35 0.7519030155047446 2119 36-37 0.1747586452558565 2119 38-39 -0.40471300916293274 2119 40-41 -0.3273327484824762 2119 42-43 -0.24975882112381953 2119 44-45 -0.5591625425537785 2119 46-47 -0.10305057957869224 2119 48-49 0.7347567068657952 2119 50-51 1.1207459589713835 2119 52-53 -0.9748528334227586 2119 54-55 -1.0697939778012966 2119 56-57 0.9065569569998715 2119 58-59 1.6927817421868738 2119 60-61 1.9892327589129692 2119 62-63 0.9147531463340428 2119 64-65 6.61212902187458E-4 2119 66-67 0.5642207886377975 2119 68-69 -0.8823894333960034 2119 70-71 -0.9971852455640118 2119 72-73 -2.331730979532292 2119 74-75 0.33931572682217137 2119 76 0.7882148697039248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 9.0 13 55.0 14 148.0 15 332.0 16 462.0 17 576.0 18 535.0 19 529.0 20 445.0 21 373.0 22 311.0 23 311.0 24 286.0 25 350.0 26 398.0 27 451.0 28 601.0 29 715.0 30 922.0 31 1165.0 32 1542.0 33 2160.0 34 2983.0 35 4808.0 36 8148.0 37 10061.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.55715178013741 22.938788257339162 11.531855090568396 31.97220487195503 2 16.064225876512566 20.325266315027406 39.215534181404486 24.39497362705554 3 15.782397352363223 25.418864412038474 32.12845175302513 26.670286482573175 4 11.937635743096493 21.48619298789947 37.790878063915606 28.785293205088426 5 13.062364256903505 36.852311511014584 35.87496121625814 14.210363015823765 6 25.310269934843316 37.682283586720445 17.698314200020686 19.309132278415557 7 24.28637914986038 32.34046954183473 23.425380080670184 19.947771227634707 8 20.98717550935981 38.29765229082635 19.386699762126383 21.328472437687456 9 22.944461681663046 16.987278932671423 22.40407487847761 37.66418450718792 10-11 23.351690971144894 26.586255041886442 27.31280380597787 22.749250180990796 12-13 22.256696659427035 24.486761816113354 26.652187403040646 26.604354121418964 14-15 21.01820250284414 26.089823146137135 23.986451546178507 28.90552280484021 16-17 21.843003412969285 27.77950149963802 25.935980970110663 24.441514117282033 18-19 20.909608025648982 27.440790154100736 26.73880442651774 24.910797393732548 20-21 20.199865549694902 27.87775364567173 26.844813320922533 25.077567483710826 22-23 22.21403454338608 27.028389699038165 26.765953045816527 23.99162271175923 24-25 21.50817044161754 28.27981176957286 26.056210569862447 24.15580721894715 26-27 20.00723963181301 28.684455476264347 27.38261454131761 23.925690350605024 28-29 21.66847657461992 27.15379046437067 28.366428793049952 22.81130416795946 30-31 21.557296514634398 27.161547212741755 28.129847967731926 23.151308304891923 32-33 19.831420002068466 29.14727479573896 27.166718378322475 23.8545868238701 34-35 21.424139000930808 28.075550729134346 27.26626331575137 23.234046954183473 36-37 21.793877339952424 27.536456717344088 26.809907953252665 23.859757989450824 38-39 20.90443686006826 27.630830489192267 28.180266832143964 23.284465818595514 40-41 21.122918605853762 26.568155962353913 27.937222049850035 24.37170338194229 42-43 20.888923363326093 26.723290929775573 28.317302720033094 24.070482986865237 44-45 19.658185955114284 27.024511324852625 28.586203330230635 24.73109938980246 46-47 20.79842796566346 26.345795842382874 28.705140138587236 24.15063605336643 48-49 19.78100113765643 27.024511324852625 29.09685593132692 24.09763160616403 50-51 19.9154514427552 26.728462095356292 28.343158547936703 25.012927913951806 52-53 19.197952218430032 26.897817768124938 28.621108697900503 25.28312131554452 54-55 18.464939497362707 27.492501809907953 28.612059158134244 25.430499534595096 56-57 19.036353294032473 26.981849208811663 28.671527562312544 25.310269934843316 58-59 19.291033198883028 26.50480918399007 28.423311614437896 25.780846002689007 60-61 19.011790257524048 26.73880442651774 28.00056882821388 26.248836487744338 62-63 18.982056055434896 26.52678663770814 28.173802875168064 26.317354431688905 64-65 19.746095769986553 26.097579894508222 27.672199813838038 26.484124521667184 66-67 19.828834419278106 26.934015927189986 26.4763677732961 26.760781880235808 68-69 19.270348536560142 27.26238494156583 26.650894611645466 26.816371910228565 70-71 20.60321646499121 26.88488985417313 26.190660874961214 26.321232805874445 72-73 20.695004654049022 26.653480194435826 25.343882511118004 27.307632640397145 74-75 20.895387320301996 26.681921605129794 25.673544316889025 26.74914675767918 76 20.969076429827282 25.925638638949216 25.573999379460133 27.531285551763368 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.5 14 1.5 15 4.0 16 5.0 17 4.0 18 5.5 19 6.0 20 5.5 21 6.0 22 10.0 23 25.5 24 41.5 25 46.0 26 71.5 27 118.0 28 163.0 29 187.0 30 237.0 31 356.5 32 483.5 33 541.0 34 663.5 35 886.0 36 1088.0 37 1190.0 38 1383.5 39 1765.5 40 2148.5 41 2411.5 42 2480.0 43 2561.5 44 2833.5 45 2974.5 46 2925.0 47 2958.0 48 2842.0 49 2547.0 50 2401.0 51 2240.0 52 1882.0 53 1553.0 54 1421.0 55 1305.5 56 983.0 57 669.5 58 563.0 59 450.5 60 301.5 61 211.0 62 157.0 63 130.0 64 88.0 65 57.5 66 37.0 67 32.0 68 30.0 69 28.0 70 23.0 71 18.0 72 15.5 73 14.5 74 12.0 75 8.0 76 6.5 77 6.0 78 5.0 79 3.0 80 4.0 81 3.5 82 2.0 83 2.0 84 1.0 85 2.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 1.0 97 2.0 98 2.5 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6515668631709587 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 38676.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.94797807425794 #Duplication Level Percentage of deduplicated Percentage of total 1 75.01982553528946 46.473265073947665 2 11.916190158186902 14.763677732961009 3 5.317417254476397 9.882097424759541 4 2.8632246754872908 7.0948391767504395 5 1.6361283859927376 5.067742269107456 6 1.1185775700154432 4.157617126900403 7 0.621895738553362 2.696762850346468 8 0.4591176593346968 2.2753128555176336 9 0.3088609708251597 1.721998138380391 >10 0.7387620518385575 5.86668735132899 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.007756748371082842 0.0 3 0.0 0.0 0.0 0.007756748371082842 0.0 4 0.0 0.0 0.0 0.012927913951804737 0.0 5 0.0 0.0 0.0 0.012927913951804737 0.0 6 0.0 0.0 0.0 0.012927913951804737 0.0 7 0.0 0.0 0.0 0.020684662322887577 0.0 8 0.0 0.0 0.0 0.020684662322887577 0.0 9 0.0 0.0 0.0 0.025855827903609473 0.0 10 0.0 0.0 0.0 0.02844141069397042 0.0 11 0.0 0.0 0.0 0.03102699348433137 0.0 12 0.0 0.0 0.0 0.049126073016858 0.0 13 0.0 0.0 0.0 0.05171165580721895 0.0 14 0.0 0.0 0.0 0.05171165580721895 0.0 15 0.0 0.0 0.0 0.05429723859757989 0.0 16 0.0 0.0 0.0 0.06463956975902369 0.0 17 0.0 0.0 0.0 0.07756748371082842 0.0 18 0.0 0.0 0.0 0.09308098045299411 0.0 19 0.0 0.0 0.0 0.09566656324335505 0.0 20 0.0 0.0 0.0 0.098252146033716 0.0 21 0.0 0.0 0.0 0.10600889440479884 0.0 22 0.0 0.0 0.0 0.11376564277588168 0.0 23 0.0 0.0 0.0 0.12410797393732548 0.0 24 0.0 0.0 0.0 0.12410797393732548 0.0 25 0.0 0.0 0.0 0.13445030509876926 0.0 26 0.0 0.0 0.0 0.15772055021201778 0.0 27 0.0 0.0 0.0 0.17064846416382254 0.0 28 0.0 0.0 0.0 0.19391870927707106 0.0 29 0.0 0.0 0.0 0.20943220601923673 0.0 30 0.0 0.0 0.0 0.2352880339228462 0.0 31 0.0 0.0 0.0 0.25855827903609474 0.0 32 0.0 0.0 0.0 0.2895852725204261 0.0 33 0.0 0.0 0.0 0.31026993484331367 0.0 34 0.0 0.0 0.0 0.35681042506981075 0.0 35 0.0 0.0 0.0 0.4498914055228048 0.0 36 0.0 0.0 0.0 0.5739993794601304 0.0 37 0.0 0.0 0.0 0.8584134863998345 0.0 38 0.0 0.0 0.0 1.220395077050367 0.0 39 0.0 0.0 0.0 1.6004757472334263 0.0 40 0.0 0.0 0.0 2.1434481332092252 0.0 41 0.0 0.0 0.0 2.7795014996380183 0.0 42 0.0 0.0 0.0 3.487951184196918 0.0 43 0.0 0.0 0.0 4.1860585375943735 0.0 44 0.0 0.0 0.0 4.904850553314717 0.0 45 0.0 0.0 0.0 5.670183059261557 0.0 46 0.0 0.0 0.0 6.461371393112008 0.0 47 0.0 0.0 0.0 7.105181507911883 0.0 48 0.0 0.0 0.0 7.803288861309339 0.0 49 0.0 0.0 0.0 8.519495294239322 0.0 50 0.0 0.0 0.0 9.176233322991003 0.0 51 0.0 0.0 0.0 9.82780018616196 0.0 52 0.0 0.0 0.0 10.518150791188335 0.0 53 0.0 0.0 0.0 11.293825628296618 0.0 54 0.0 0.0 0.0 11.929878994725412 0.0 55 0.0 0.0 0.0 12.648671010445755 0.0 56 0.0 0.0 0.0 13.295066708035991 0.0 57 0.0 0.0 0.0 13.913020994932257 0.0 58 0.0 0.0 0.0 14.455993380908057 0.0 59 0.0 0.0 0.0 15.086875581756129 0.0 60 0.0 0.0 0.0 15.69190195470059 0.0 61 0.0 0.0 0.0 16.219360843934222 0.0 62 0.0 0.0 0.0 16.73647740200641 0.0 63 0.0 0.0 0.0 17.31047678146654 0.0 64 0.0 0.0 0.0 17.912917571620643 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGATTC 15 0.002209438 69.99353 56 TTAATGC 15 0.002209438 69.99353 22 ATGCCAT 15 0.002209438 69.99353 43 TAATGCA 15 0.002209438 69.99353 23 GTCCTAC 20 0.0067288317 52.836914 1 CTATGGA 20 0.006904092 52.495148 4 GAGCTTT 20 0.006904092 52.495148 9 GGGTCTT 20 0.006904092 52.495148 28 AGCTTTA 20 0.006904092 52.495148 10 AGAAGTA 20 0.006904092 52.495148 48 CTAACTA 20 0.006904092 52.495148 9 AATGCAA 20 0.006904092 52.495148 24 TGATTCA 20 0.006904092 52.495148 57 GAGCCTG 20 0.006904092 52.495148 9 CCTATGG 20 0.006904092 52.495148 3 ATGCAAA 20 0.006904092 52.495148 25 AGTACCT 20 0.006904092 52.495148 33 GCTTTAA 20 0.006904092 52.495148 11 TGCAAAC 20 0.006904092 52.495148 26 TTATTCC 45 0.004259564 31.108236 26 >>END_MODULE