FastQCFastQC Report
Wed 25 May 2016
SRR1780726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38676
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT5731.4815389388768228No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT3380.8739269831420001Illumina PCR Primer Index 9 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC860.2223601199710415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC600.15513496742165683No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT470.12152239114696453No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT400.1034233116144379No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTC200.006901569752.516
TGTCAAA200.006901569752.512
AATTATT400.002390719735.042
TTAGTAT450.004257638531.1111093
CGTCTTC1650.029.6969748
TCTCGTA1551.8189894E-1229.3548438
GATCTCG1551.8189894E-1229.3548436
TATGCCG1800.029.16666443
CGTATGC1800.029.16666441
TCGTATG1800.029.16666440
CCGTCTT1700.028.8235347
GTCTTCT1700.028.8235349
ATCTCGT1601.8189894E-1228.437537
ATGCCGT1850.028.37837844
TAAGATC1502.7284841E-1128.033
GCCGTCT1750.028.046
TCTTCTG1750.028.050
GTGGGGA500.0071258327.9999984
TGCCGTC1900.027.63157845
AGATCTC1653.6379788E-1227.57575835