##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780726_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38676 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.589228462095356 34.0 34.0 34.0 31.0 34.0 2 33.21253490536767 34.0 34.0 34.0 32.0 34.0 3 33.40368704105906 34.0 34.0 34.0 33.0 34.0 4 33.36273140965974 34.0 34.0 34.0 33.0 34.0 5 33.36826455683111 34.0 34.0 34.0 34.0 34.0 6 37.06882821387941 38.0 37.0 38.0 36.0 38.0 7 36.73738235598304 38.0 37.0 38.0 34.0 38.0 8 36.68931637191023 38.0 37.0 38.0 34.0 38.0 9 36.51473782190506 38.0 37.0 38.0 34.0 38.0 10-11 36.172316165063606 38.0 36.5 38.0 33.0 38.0 12-13 36.06063191643396 38.0 37.0 38.0 32.5 38.0 14-15 36.30809804529941 38.0 37.0 38.0 33.5 38.0 16-17 36.474881063191646 38.0 37.0 38.0 34.0 38.0 18-19 36.46840417830179 38.0 37.0 38.0 34.0 38.0 20-21 36.19596131968146 38.0 37.0 38.0 33.0 38.0 22-23 36.21545661392078 38.0 37.0 38.0 33.0 38.0 24-25 35.50919174681974 37.5 36.0 38.0 29.0 38.0 26-27 35.75845485572448 38.0 36.0 38.0 31.5 38.0 28-29 35.59226652187403 38.0 36.0 38.0 31.0 38.0 30-31 35.25049126073017 38.0 36.0 38.0 29.0 38.0 32-33 35.82185334574413 38.0 36.5 38.0 31.5 38.0 34-35 35.82098717550936 38.0 36.5 38.0 32.5 38.0 36-37 35.97561795428689 38.0 37.0 38.0 33.5 38.0 38-39 35.56286844554762 38.0 36.5 38.0 30.0 38.0 40-41 35.058589306029575 37.0 35.5 38.0 29.0 38.0 42-43 35.49449270865653 37.5 36.0 38.0 30.0 38.0 44-45 35.71029837625401 38.0 36.0 38.0 31.5 38.0 46-47 36.038344192781054 38.0 37.0 38.0 33.5 38.0 48-49 35.84015927189989 38.0 36.5 38.0 31.5 38.0 50-51 35.96914106939704 38.0 37.0 38.0 32.5 38.0 52-53 35.86227893267143 38.0 37.0 38.0 32.0 38.0 54-55 36.08554400661909 38.0 37.0 38.0 34.0 38.0 56-57 36.13883286792843 38.0 37.0 38.0 34.0 38.0 58-59 36.21841710621574 38.0 37.0 38.0 34.0 38.0 60-61 35.838142517323405 38.0 36.5 38.0 32.5 38.0 62-63 36.164094011790255 38.0 37.0 38.0 34.0 38.0 64-65 36.16073275416279 38.0 37.0 38.0 34.0 38.0 66-67 35.47402782087082 38.0 36.0 38.0 31.0 38.0 68-69 34.750297342020886 37.5 35.0 38.0 26.0 38.0 70-71 34.69261298996794 37.0 35.0 38.0 26.0 38.0 72-73 34.34759282242217 37.0 34.5 38.0 24.5 38.0 74-75 35.03984383079946 37.5 36.0 38.0 27.0 38.0 76 34.49581135587962 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43055671267166673 1101 2 0.6656385394287625 1101 3 0.2590558371402025 1101 4 0.48584533794521434 1101 5 0.36983776948042646 1101 6 -1.3946155499594326 1101 7 0.314912543269692 1101 8 0.7414115892461624 1101 9 0.24034255303268992 1101 10-11 0.5149784723039517 1101 12-13 0.5074690256051184 1101 14-15 0.4962194767745274 1101 16-17 -0.12251255107535286 1101 18-19 -0.22302940253245396 1101 20-21 0.2133832767303474 1101 22-23 0.9761831006036843 1101 24-25 -1.1732440036925453 1101 26-27 -0.27011867218470087 1101 28-29 0.6391617884747163 1101 30-31 0.8971437498725763 1101 32-33 -0.025815331133408392 1101 34-35 0.4714701699429895 1101 36-37 0.45001319239921145 1101 38-39 0.414401654220363 1101 40-41 0.9377218239265019 1101 42-43 0.7328701968333817 1101 44-45 0.4782024018038413 1101 46-47 0.2378038604540933 1101 48-49 -2.6845632375189226 1101 50-51 -1.5108248865936105 1101 52-53 -1.0644416130950631 1101 54-55 -0.8578154580633139 1101 56-57 -0.7401340550510724 1101 58-59 -1.2702239561718685 1101 60-61 -1.5196418024427345 1101 62-63 -0.8651943615686051 1101 64-65 -1.3205502927833521 1101 66-67 -1.3971756462206244 1101 68-69 -0.06798078505943295 1101 70-71 -0.6790852606944782 1101 72-73 -1.3633790361013283 1101 74-75 -0.972617822128818 1101 76 -1.784158993560304 1102 1 -1.3619725402536247 1102 2 0.223244300004815 1102 3 0.430252956852172 1102 4 0.630129766388059 1102 5 0.3369844841518983 1102 6 0.39395330692625663 1102 7 0.3213931913345007 1102 8 0.5411415622434603 1102 9 0.6034388626636584 1102 10-11 0.6440513795946856 1102 12-13 0.42119539824238217 1102 14-15 -0.0034204872218737137 1102 16-17 0.147019402119966 1102 18-19 0.06792469287707803 1102 20-21 0.7879557339760694 1102 22-23 0.37100758305192727 1102 24-25 -4.712397919084083 1102 26-27 -1.7248441447319536 1102 28-29 -0.04288141584572003 1102 30-31 1.2887279082884149 1102 32-33 0.4882710775074486 1102 34-35 0.4224152644524466 1102 36-37 -3.3931011190319254 1102 38-39 -0.7121510010451644 1102 40-41 0.37935098684278756 1102 42-43 0.868198729686668 1102 44-45 0.7530448860522654 1102 46-47 1.2299730773757815 1102 48-49 -2.9351933005252278 1102 50-51 -0.7151453186368215 1102 52-53 -0.7829834672804807 1102 54-55 0.008611184600958666 1102 56-57 0.5798029386482995 1102 58-59 -0.2687838121574657 1102 60-61 -6.058390677330223 1102 62-63 -2.8031331554479877 1102 64-65 -0.4436376015142258 1102 66-67 0.8992689982438264 1102 68-69 -1.3585298399649233 1102 70-71 -1.9361509672651351 1102 72-73 -6.338131511348852 1102 74-75 -3.052950855432151 1102 76 -0.6533759152524681 1103 1 0.8466521683710866 1103 2 0.557743134503653 1103 3 0.5480462746454933 1103 4 0.6339370701953584 1103 5 0.6349642821316905 1103 6 0.619516632489578 1103 7 1.0693341392754476 1103 8 1.0030685241704234 1103 9 0.4388700980948883 1103 10-11 0.7292883648316746 1103 12-13 -0.34185821481123213 1103 14-15 0.056175472374086155 1103 16-17 0.6033908084913762 1103 18-19 1.1279480029003892 1103 20-21 0.017986814007151963 1103 22-23 1.0325848946292382 1103 24-25 -2.34837305505922 1103 26-27 0.40716828728047716 1103 28-29 0.2731636501993506 1103 30-31 1.1746253441858556 1103 32-33 1.3429330821694592 1103 34-35 0.3249016669388496 1103 36-37 -0.7692471951780107 1103 38-39 0.47021108131691136 1103 40-41 0.10464236213416456 1103 42-43 0.7330394445273853 1103 44-45 1.610892593899969 1103 46-47 1.1591883065910125 1103 48-49 -2.0683323836643055 1103 50-51 0.13295102945952664 1103 52-53 0.6448331605361446 1103 54-55 0.5822708582606282 1103 56-57 0.6873009961463552 1103 58-59 -0.12014356351721744 1103 60-61 -4.356487025426567 1103 62-63 -0.7391471414619772 1103 64-65 0.7220578141811913 1103 66-67 0.5123614613362903 1103 68-69 0.9663341848990967 1103 70-71 1.1234449923308247 1103 72-73 -4.862490485707827 1103 74-75 -1.58694463942593 1103 76 -3.3053572672338234 1104 1 0.5802462578973788 1104 2 0.39889915362577 1104 3 0.41361197071966416 1104 4 0.007325452058317694 1104 5 0.1698042068021266 1104 6 -0.04657057597051306 1104 7 0.7500526674516053 1104 8 0.7268118241849137 1104 9 1.1857192940966286 1104 10-11 -0.06989315045153432 1104 12-13 -0.14868133519367888 1104 14-15 0.7275502369691935 1104 16-17 0.23862186745125058 1104 18-19 0.618386942491874 1104 20-21 0.25806359221952135 1104 22-23 -0.2570355602454555 1104 24-25 0.41741718107000025 1104 26-27 1.3938769646671219 1104 28-29 1.5846224362344046 1104 30-31 1.1200838404918088 1104 32-33 1.227331169957381 1104 34-35 0.05223036460652963 1104 36-37 1.246036015020458 1104 38-39 1.7372326661351067 1104 40-41 1.8410613104175226 1104 42-43 0.8591078205957601 1104 44-45 0.7832451942186722 1104 46-47 0.7211903346947395 1104 48-49 0.4932350507844774 1104 50-51 1.1496466690365281 1104 52-53 0.924566609761122 1104 54-55 0.6659301368351649 1104 56-57 0.4522220449657155 1104 58-59 -0.2389687120033841 1104 60-61 1.0293597078777879 1104 62-63 0.45218271512211317 1104 64-65 1.1769325063440164 1104 66-67 0.376695808721486 1104 68-69 1.6633499751352332 1104 70-71 1.794819756925932 1104 72-73 0.9169532344138602 1104 74-75 0.19666393655553094 1104 76 -0.08588747149283904 1105 1 0.9461878033397895 1105 2 0.35746047752807897 1105 3 0.5273180964369999 1105 4 0.3945535315992217 1105 5 -0.5295151579382988 1105 6 0.07634414443520399 1105 7 0.9925456609121639 1105 8 -0.5716000268760766 1105 9 1.0237681396858491 1105 10-11 0.2920112936963335 1105 12-13 -0.6572226404118808 1105 14-15 -0.12317710855329977 1105 16-17 -0.061391478338158834 1105 18-19 0.6336011424117984 1105 20-21 0.13051120999611499 1105 22-23 0.1644578335887914 1105 24-25 0.8377094538579328 1105 26-27 0.30335914803826824 1105 28-29 0.7260878039109002 1105 30-31 1.171798774430151 1105 32-33 1.1467893309075947 1105 34-35 -1.0353513783535675 1105 36-37 0.5026755452486711 1105 38-39 -0.48187183139591383 1105 40-41 0.32491290523935135 1105 42-43 0.17603695445402678 1105 44-45 0.8658222661524846 1105 46-47 0.8061362842476498 1105 48-49 -0.10652472500625976 1105 50-51 0.6739026412772802 1105 52-53 0.2872384367996901 1105 54-55 0.7296562812366005 1105 56-57 0.10940207966476123 1105 58-59 -0.11395202976662944 1105 60-61 0.47264010291316083 1105 62-63 -0.6464760330428376 1105 64-65 0.46026361537911953 1105 66-67 0.054995555151485576 1105 68-69 0.11577223591195462 1105 70-71 0.5897617027420239 1105 72-73 0.5764354177850066 1105 74-75 -0.5975106263698677 1105 76 -0.22681900759319973 1106 1 -1.052881631162716 1106 2 0.07778975455027393 1106 3 0.5393438659430814 1106 4 0.41194794820624026 1106 5 0.1188026659700796 1106 6 0.657589670562615 1106 7 -0.5967886268473208 1106 8 0.9047779258798272 1106 9 -0.04201568279088974 1106 10-11 0.5167786523219604 1106 12-13 1.0030135800605606 1106 14-15 -0.6170568508582335 1106 16-17 0.3879284930290581 1106 18-19 0.6542883292407211 1106 20-21 0.3925011885215284 1106 22-23 0.9437348557791978 1106 24-25 -0.10330700999317344 1106 26-27 1.1978831279953184 1106 28-29 1.0389367659724655 1106 30-31 1.1296369991975084 1106 32-33 0.22463471387109024 1106 34-35 0.7315061735433517 1106 36-37 1.2887170627862474 1106 38-39 0.32421263531846733 1106 40-41 1.3520782595700567 1106 42-43 1.195471456959396 1106 44-45 0.9575903405977257 1106 46-47 1.1163367137394218 1106 48-49 1.5420794267474989 1106 50-51 0.8212183177268173 1106 52-53 0.8624710781740674 1106 54-55 0.7222475482373198 1106 56-57 0.3707120295573887 1106 58-59 1.026670733297081 1106 60-61 1.1097911408515984 1106 62-63 1.1468668445520152 1106 64-65 -0.907273965150587 1106 66-67 0.6356326346074539 1106 68-69 1.6187428873078034 1106 70-71 0.25021266909850226 1106 72-73 0.9891412159238726 1106 74-75 0.13795823547694397 1106 76 0.14662408474752908 1107 1 1.0471183688372818 1107 2 -0.16105322065634198 1107 3 0.14760832875300167 1107 4 0.2888074523384745 1107 5 0.07665390563949614 1107 6 -0.16059214761919804 1107 7 0.5594097202601205 1107 8 0.4130423886897461 1107 9 -0.6064784927082414 1107 10-11 0.8969439415781579 1107 12-13 0.33731110072172044 1107 14-15 0.08418281856324938 1107 16-17 0.41189543517781857 1107 18-19 0.6732965937035331 1107 20-21 0.5288648248851686 1107 22-23 0.055718326853572364 1107 24-25 0.5479326594283123 1107 26-27 0.09912279741680408 1107 28-29 1.1038127990303153 1107 30-31 0.19327336283387098 1107 32-33 1.0944694246148856 1107 34-35 -0.3131219256301989 1107 36-37 1.1126840049350122 1107 38-39 0.04487379234326028 1107 40-41 0.34918569758659146 1107 42-43 0.7372069941494814 1107 44-45 0.3782514976225144 1107 46-47 0.8597251434914881 1107 48-49 0.928029839970641 1107 50-51 0.7683257557433478 1107 52-53 0.10379339222365047 1107 54-55 0.35902440774145816 1107 56-57 0.4355880626152455 1107 58-59 0.08162941098302667 1107 60-61 0.8883035375458093 1107 62-63 0.9700073404197767 1107 64-65 0.7898334728659435 1107 66-67 0.09472354369837177 1107 68-69 0.36708999474581816 1107 70-71 -0.09648154577753587 1107 72-73 0.4920337779073449 1107 74-75 -1.3360087066718123 1107 76 -0.34180566731858875 1108 1 0.012449647727883928 1108 2 0.4158483061681437 1108 3 0.3797136656349167 1108 4 0.5412237571430651 1108 5 0.5342109864631439 1108 6 0.029700610470165145 1108 7 -0.12282868848060247 1108 8 -0.04437461649305163 1108 9 -0.40750104488642336 1108 10-11 0.6038356631077804 1108 12-13 1.5462339190436083 1108 14-15 0.2783976945963076 1108 16-17 0.3530286471122608 1108 18-19 0.5717767730003516 1108 20-21 1.1097585074737637 1108 22-23 0.3573712194155618 1108 24-25 1.0445358251377925 1108 26-27 -0.7713772726210166 1108 28-29 -0.16961485190118708 1108 30-31 0.8065245184579126 1108 32-33 0.9773311699573739 1108 34-35 0.442060873081104 1108 36-37 1.2121377099357176 1108 38-39 0.9067241915588369 1108 40-41 0.769027412112429 1108 42-43 -0.2002142133025444 1108 44-45 1.0713807874390113 1108 46-47 0.7974615211354106 1108 48-49 0.8322181016319377 1108 50-51 1.0013415842907634 1108 52-53 0.3101598301001047 1108 54-55 -0.2789851174021223 1108 56-57 0.6090017059826565 1108 58-59 0.08306518630169535 1108 60-61 1.0335969960133866 1108 62-63 0.7699793252916081 1108 64-65 0.6345596249880856 1108 66-67 1.1182212324502956 1108 68-69 1.5277567547962434 1108 70-71 -0.26873956510797115 1108 72-73 -0.934741680840375 1108 74-75 -1.002488605817355 1108 76 0.6344515115580052 1109 1 -1.2302709085519936 1109 2 0.2415038182643343 1109 3 0.44548217208139107 1109 4 -0.041276605018310875 1109 5 0.35291300008041304 1109 6 -0.004414991442047267 1109 7 -0.3580162880749782 1109 8 0.4560599771618783 1109 9 0.8747675339923262 1109 10-11 0.498519134062434 1109 12-13 0.08976571681269974 1109 14-15 0.7527566689552856 1109 16-17 0.9068096119101767 1109 18-19 0.40145227640466885 1109 20-21 1.073542369562709 1109 22-23 0.8146361766805157 1109 24-25 -0.19880040548657263 1109 26-27 -0.4090710289588415 1109 28-29 -1.8208534438177466 1109 30-31 0.4053945749550891 1109 32-33 1.0095997488361235 1109 34-35 0.004388846426024884 1109 36-37 1.1153681894373761 1109 38-39 1.3590999702058042 1109 40-41 0.4593432168350162 1109 42-43 0.9381676496555897 1109 44-45 0.9014908844982656 1109 46-47 0.7361113835140927 1109 48-49 0.38465906932714944 1109 50-51 0.39363479014328817 1109 52-53 -0.8466198309168433 1109 54-55 -0.507472731482963 1109 56-57 0.0035403123856738716 1109 58-59 -0.17142561479926854 1109 60-61 0.7845386155990752 1109 62-63 0.6198272175123876 1109 64-65 0.8417159338393105 1109 66-67 0.5550965040713294 1109 68-69 0.2312914498563643 1109 70-71 0.6319920008778297 1109 72-73 0.039218916001573234 1109 74-75 -0.3989104513917425 1109 76 0.1561811943046365 1110 1 -1.5278417587544197 1110 2 -0.7911097669808314 1110 3 -0.20116650088306898 1110 4 0.1674503405507366 1110 5 0.4127420599094691 1110 6 0.25806813946213936 1110 7 0.45552397283370283 1110 8 1.0626726627219298 1110 9 1.233741892966684 1110 10-11 -0.7934286842011744 1110 12-13 0.6328381414640702 1110 14-15 0.7915555893331501 1110 16-17 0.23170839733527515 1110 18-19 -0.01780098495385829 1110 20-21 0.7242396255231185 1110 22-23 -0.822453341987945 1110 24-25 -0.9938971216359178 1110 26-27 1.3519501136412515 1110 28-29 0.26652847251153844 1110 30-31 -0.6779387583782537 1110 32-33 0.899275862196447 1110 34-35 0.5663546583918446 1110 36-37 1.3411891521004478 1110 38-39 1.0708633530218137 1110 40-41 -1.6163427930615129 1110 42-43 0.2143709784904999 1110 44-45 0.8476222385243588 1110 46-47 0.7561293772162969 1110 48-49 1.6527652321701467 1110 50-51 -0.48906876361289164 1110 52-53 0.7761871866270198 1110 54-55 -0.28277637520765353 1110 56-57 0.8997391427950276 1110 58-59 -0.5888795059373706 1110 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2116 50-51 -1.2051030353387233 2116 52-53 -1.0384802960331214 2116 54-55 -1.1389575257584497 2116 56-57 -0.32527105712336635 2116 58-59 -2.961278526745204 2116 60-61 -1.9370376117911121 2116 62-63 -0.2145496459342482 2116 64-65 -2.9864494408376885 2116 66-67 -0.5252553146525827 2116 68-69 -1.390453730028348 2116 70-71 1.1945044238553777 2116 72-73 -0.0044105811162964415 2116 74-75 -0.11531872012559319 2116 76 -0.7451306721235085 2117 1 1.2931076736501197 2117 2 0.5788592732668505 2117 3 0.5671513525741005 2117 4 0.3761190712008897 2117 5 0.06372245206633664 2117 6 0.45652015184604267 2117 7 0.9588263464147104 2117 8 0.6165426317621794 2117 9 -0.46821889134704264 2117 10-11 -0.9682480856459534 2117 12-13 -1.03522171405708 2117 14-15 0.17839769459631327 2117 16-17 -1.209932469537783 2117 18-19 -1.706139478245909 2117 20-21 -0.7647180628153691 2117 22-23 -0.23086407470209025 2117 24-25 -2.426301662399588 2117 26-27 -2.4141489575661765 2117 28-29 -1.9276407741344883 2117 30-31 -0.8906838564174606 2117 32-33 -1.8098572647385396 2117 34-35 0.22027622701929062 2117 36-37 -1.3364166270533246 2117 38-39 0.0669933839815755 2117 40-41 -0.09792174042993196 2117 42-43 0.020872526478108 2117 44-45 -1.1044417449637791 2117 46-47 -0.9128076712873181 2117 48-49 -0.9033751187070393 2117 50-51 -1.2499046769255884 2117 52-53 0.7945566396713915 2117 54-55 -0.21652250523861483 2117 56-57 -0.14025053728753534 2117 58-59 -1.3826875554729767 2117 60-61 -0.4853960249238014 2117 62-63 0.26130534722580734 2117 64-65 -0.27919909884042937 2117 66-67 -0.2726561354460202 2117 68-69 0.6318444915858805 2117 70-71 -0.9072739619175394 2117 72-73 1.1353979004158532 2117 74-75 1.231808502856623 2117 76 0.7803139243197208 2118 1 -0.19900136508732658 2118 2 -0.14926124323243073 2118 3 -1.461543052017003 2118 4 0.3888880812439339 2118 5 0.05095344202329244 2118 6 -0.1982861609229687 2118 7 0.7965594884520186 2118 8 -0.022051342412858332 2118 9 -1.7865833102853443 2118 10-11 -1.6716914141968857 2118 12-13 -1.973815688232122 2118 14-15 0.39425135313289417 2118 16-17 -2.8295881366826947 2118 18-19 -0.7624522472559576 2118 20-21 -1.0127094544939936 2118 22-23 0.674444390147265 2118 24-25 0.4538991984325449 2118 26-27 -4.22218339085169 2118 28-29 -1.3344556952248752 2118 30-31 -2.169808677077434 2118 32-33 -0.0785803637342326 2118 34-35 -0.17348273998214125 2118 36-37 -4.264608879564079 2118 38-39 -0.9053882515994971 2118 40-41 -0.7052388136006655 2118 42-43 0.23715660108356218 2118 44-45 -3.130768861176115 2118 46-47 -0.17091384058430492 2118 48-49 0.2088200032441776 2118 50-51 -2.177451305333051 2118 52-53 -2.175888123599769 2118 54-55 -2.1556903675054713 2118 56-57 0.16139939097646305 2118 58-59 -1.92199888976279 2118 60-61 -2.137548105268138 2118 62-63 -3.1073460157584094 2118 64-65 -0.43809436426366943 2118 66-67 0.18674128205757512 2118 68-69 -2.334583055042529 2118 70-71 -0.867747419593293 2118 72-73 -0.11374126744641444 2118 74-75 -1.0174741370286 2118 76 -0.7859701502857277 2119 1 0.8261393478582661 2119 2 -1.632000455239936 2119 3 -0.038278511679294525 2119 4 -1.0908492545909638 2119 5 -0.14623229906488433 2119 6 -2.178773965801021 2119 7 -2.4520333820920754 2119 8 -0.9029143818124794 2119 9 -1.571386312161522 2119 10-11 -1.0818227462794425 2119 12-13 -0.7316018045548205 2119 14-15 -0.8985253823267669 2119 16-17 -0.570113465012902 2119 18-19 -0.49256481761242554 2119 20-21 -1.7221841261637891 2119 22-23 -0.0887826267382863 2119 24-25 0.2917978851117269 2119 26-27 0.18494606505825573 2119 28-29 -0.3362380592023584 2119 30-31 1.523343292903796 2119 32-33 0.07113821037458479 2119 34-35 0.572764914802093 2119 36-37 -0.5812130071438162 2119 38-39 -3.0212419101360766 2119 40-41 -2.3056140481222442 2119 42-43 -1.098584487096545 2119 44-45 -0.48226979926241853 2119 46-47 -0.009187761785049986 2119 48-49 -1.4264520417839677 2119 50-51 0.387651884160384 2119 52-53 -0.16200444630145938 2119 54-55 -0.084395808406029 2119 56-57 -1.713553704708339 2119 58-59 0.535411992038334 2119 60-61 0.30419673525719304 2119 62-63 -0.992993295308132 2119 64-65 -1.7488823567589833 2119 66-67 -1.0115701625953406 2119 68-69 0.006505125070042084 2119 70-71 0.43797490686073814 2119 72-73 1.3657146424973021 2119 74-75 0.14809809561680964 2119 76 -1.4079213697979256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 5.0 23 12.0 24 14.0 25 37.0 26 81.0 27 153.0 28 250.0 29 459.0 30 652.0 31 1100.0 32 1705.0 33 2614.0 34 3921.0 35 5709.0 36 9504.0 37 12457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.86141276243666 23.916640810838764 11.671320715689317 29.550625711035266 2 17.03640500568828 19.655600372323924 39.68093908366946 23.627055538318338 3 16.167649188127005 26.429827283069603 31.52342538008067 25.879098148722722 4 12.211707518874753 20.733788395904437 39.15089461164546 27.90360947357534 5 11.469645258041163 37.92791395180474 37.27376150584342 13.328679284310684 6 26.40397145516599 40.68931637191023 18.086151618574828 14.82056055434895 7 25.041369324645775 31.03475023270245 23.83131657875685 20.09256386389492 8 21.416382252559725 37.60988726859034 21.292274278622404 19.681456200227533 9 22.791912297031754 14.911055952011584 22.773813217499224 39.523218533457445 10-11 23.66454648877857 26.80473678767194 27.939807632640395 21.59090909090909 12-13 23.394353087185852 23.294808149756953 27.64117282035371 25.669665942703485 14-15 20.788085634502014 25.63605336642879 23.619298789947255 29.956562209121934 16-17 22.23730478849933 28.35091529630779 25.434377908780636 23.977402006412245 18-19 21.77965663460544 27.469231564794704 26.66382252559727 24.087289275002586 20-21 20.874702657979107 26.8473989037129 26.206174371703383 26.071724066604613 22-23 21.663305409039197 28.59913124418244 26.82800703278519 22.909556313993175 24-25 21.961940221325886 28.87578860275106 26.709070224428586 22.453200951494466 26-27 21.168166304685077 28.831833695314923 27.691591684765747 22.308408315234253 28-29 21.243148205605543 27.120177888095974 28.816320198572758 22.820353707725722 30-31 21.461629951391043 27.36968662736581 29.214499948288342 21.954183472954806 32-33 20.91348639983452 28.34703692212225 27.776915916847656 22.962560761195576 34-35 21.03759437377185 30.041886441203847 27.78337987382356 21.137139311200745 36-37 22.728565518667907 27.86224014892957 26.84610611231772 22.563088220084808 38-39 21.420260626745268 27.34512359085738 27.59463233012721 23.63998345227014 40-41 20.299410487123797 26.883597062777948 29.198986451546176 23.618005998552075 42-43 21.994260006205398 26.38328679284311 29.35929258454856 22.26316061640294 44-45 19.92967214810218 27.307632640397145 29.249405315958217 23.513289895542457 46-47 21.72535939600786 25.527458889233635 29.314044885717237 23.433136829041263 48-49 20.729910021718894 25.593391250387835 29.268797186885926 24.407901541007345 50-51 19.38282138794084 26.092408728927502 29.921656841452066 24.603113041679595 52-53 19.1604612679698 25.98769262591788 31.966852828627573 22.884993277484746 54-55 18.50372323921812 26.447926362602132 30.437480608129075 24.61086979005068 56-57 20.298117695728617 26.22556624263109 28.95982004343779 24.516496018202503 58-59 18.706691488261455 27.2908263522598 30.075499017478542 23.926983142000207 60-61 19.73316785603475 28.5086358465198 28.288861309339126 23.46933498810632 62-63 17.752611438618267 30.09876926259179 27.553263005481437 24.595356293308512 64-65 18.579997931533768 30.91969179853139 25.89073327127935 24.6095769986555 66-67 18.546385355259076 30.45428689626642 26.164805047057605 24.8345227014169 68-69 18.457182748991624 30.52409763160616 25.421449994828833 25.597269624573375 70-71 18.758403144068673 29.545454545454547 25.851949529423933 25.84419278105285 72-73 18.934222773813218 28.518978177681248 25.801530665011896 26.74526838349364 74-75 19.59871755093598 28.219050573999375 25.90366118523115 26.278570689833487 76 19.412555590029992 28.46726652187403 26.272106732857587 25.84807115523839 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 2.5 17 5.0 18 2.5 19 1.0 20 4.5 21 7.0 22 14.0 23 37.0 24 50.5 25 48.0 26 72.5 27 113.0 28 162.5 29 196.0 30 251.5 31 365.0 32 521.0 33 619.0 34 731.0 35 956.5 36 1130.0 37 1190.0 38 1408.0 39 1895.5 40 2496.0 41 2916.0 42 3005.0 43 3028.5 44 3052.0 45 3093.0 46 3134.0 47 3094.5 48 2925.0 49 2584.0 50 2373.0 51 2130.0 52 1656.5 53 1289.5 54 1153.0 55 1018.5 56 717.0 57 465.0 58 380.0 59 274.5 60 125.0 61 58.5 62 36.0 63 23.5 64 6.5 65 1.0 66 0.0 67 0.0 68 0.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 38676.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.0 #Duplication Level Percentage of deduplicated Percentage of total 1 64.56200227531286 32.28100113765643 2 15.828937842589719 15.828937842589719 3 7.75157720550212 11.627365808253181 4 4.1472747957389595 8.294549591477919 5 2.4356189885200124 6.089047471300031 6 1.4324128658599649 4.297238597579894 7 1.1893680835660356 4.1627882924811255 8 0.7187920157203433 2.8751680628813734 9 0.5584858827179646 2.513186472230841 >10 1.3548453821491364 9.297755714137965 >50 0.01034233116144379 0.3774950873926983 >100 0.005171165580721895 0.8739269831420001 >500 0.005171165580721895 1.4815389388768228 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 573 1.4815389388768228 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 338 0.8739269831420001 Illumina PCR Primer Index 9 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 86 0.2223601199710415 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 60 0.15513496742165683 No Hit TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT 47 0.12152239114696453 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT 40 0.1034233116144379 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.007756748371082842 0.0 3 0.0 0.0 0.0 0.007756748371082842 0.0 4 0.0 0.0 0.0 0.015513496742165685 0.0 5 0.0 0.0 0.0 0.015513496742165685 0.0 6 0.0 0.0 0.0 0.015513496742165685 0.0 7 0.0 0.0 0.0 0.023270245113248527 0.0 8 0.0 0.0 0.0 0.025855827903609473 0.0 9 0.0 0.0 0.0 0.03102699348433137 0.0 10 0.0 0.0 0.0 0.033612576274692316 0.0 11 0.0 0.0 0.0 0.03619815906505326 0.0 12 0.0 0.0 0.0 0.05688282138794084 0.0 13 0.0 0.0 0.0 0.05946840417830179 0.0 14 0.0 0.0 0.0 0.05946840417830179 0.0 15 0.0 0.0 0.0 0.05946840417830179 0.0 16 0.0 0.0 0.0 0.06981073533974558 0.0 17 0.0 0.0 0.0 0.07756748371082842 0.0 18 0.0 0.0 0.0 0.09566656324335505 0.0 19 0.0 0.0 0.0 0.098252146033716 0.0 20 0.0 0.0 0.0 0.10083772882407695 0.0 21 0.0 0.0 0.0 0.10600889440479884 0.0 22 0.0 0.0 0.0 0.10859447719515979 0.0 23 0.0 0.0 0.0 0.11893680835660358 0.0 24 0.0 0.0 0.0 0.11893680835660358 0.0 25 0.0 0.0 0.0 0.12669355672768642 0.0 26 0.0 0.0 0.0 0.1370358878891302 0.002585582790360947 27 0.0 0.0 0.0 0.14996380184093494 0.002585582790360947 28 0.0 0.0 0.0 0.17581962974454443 0.002585582790360947 29 0.0 0.0 0.0 0.1913331264867101 0.002585582790360947 30 0.0 0.0 0.0 0.21977453718068052 0.002585582790360947 31 0.0 0.0 0.0 0.24304478229392906 0.002585582790360947 32 0.0 0.0 0.0 0.2740717757782604 0.002585582790360947 33 0.0 0.0 0.0 0.294756438101148 0.002585582790360947 34 0.0 0.0 0.0 0.35163925948908886 0.002585582790360947 35 0.0 0.0 0.0 0.4369634915710001 0.002585582790360947 36 0.0 0.0 0.0 0.5739993794601304 0.002585582790360947 37 0.0 0.0 0.0 0.8506567380287516 0.002585582790360947 38 0.0 0.0 0.0 1.1997104147274795 0.002585582790360947 39 0.0 0.0 0.0 1.5797910849105388 0.002585582790360947 40 0.0 0.0 0.0 2.117592305305616 0.002585582790360947 41 0.0 0.0 0.0 2.763988002895853 0.002585582790360947 42 0.0 0.0 0.0 3.4776088530354743 0.002585582790360947 43 0.0 0.0 0.0 4.157617126900403 0.002585582790360947 44 0.0 0.0 0.0 4.871237977040025 0.002585582790360947 45 0.0 0.0 0.0 5.626228151825422 0.002585582790360947 46 0.0 0.0 0.0 6.422587651256594 0.002585582790360947 47 0.0 0.0 0.0 7.0948391767504395 0.002585582790360947 48 0.0 0.0 0.0 7.808460026890061 0.002585582790360947 49 0.0 0.0 0.0 8.56862136725618 0.002585582790360947 50 0.0 0.0 0.0 9.264143137863275 0.002585582790360947 51 0.0 0.0 0.0 9.926052332195678 0.002585582790360947 52 0.0 0.0 0.0 10.650015513496742 0.002585582790360947 53 0.0 0.0 0.0 11.428275933395387 0.002585582790360947 54 0.0 0.0 0.0 12.082428379356706 0.002585582790360947 55 0.0 0.0 0.0 12.808977143448134 0.002585582790360947 56 0.0 0.0 0.0 13.439859344296204 0.002585582790360947 57 0.0 0.0 0.0 14.047471300031027 0.002585582790360947 58 0.0 0.0 0.0 14.595614851587548 0.002585582790360947 59 0.0 0.0 0.0 15.247181714758506 0.002585582790360947 60 0.0 0.0 0.0 15.875478332816217 0.002585582790360947 61 0.0 0.0 0.0 16.413279553211293 0.002585582790360947 62 0.0 0.0 0.0 16.961423104767814 0.002585582790360947 63 0.0 0.0 0.0 17.54576481538939 0.002585582790360947 64 0.0 0.0 0.0 18.137863274382045 0.002585582790360947 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTGTC 20 0.0069015697 52.5 16 TGTCAAA 20 0.0069015697 52.5 12 AATTATT 40 0.0023907197 35.0 42 TTAGTAT 45 0.0042576385 31.111109 3 CGTCTTC 165 0.0 29.69697 48 TCTCGTA 155 1.8189894E-12 29.35484 38 GATCTCG 155 1.8189894E-12 29.35484 36 TATGCCG 180 0.0 29.166664 43 CGTATGC 180 0.0 29.166664 41 TCGTATG 180 0.0 29.166664 40 CCGTCTT 170 0.0 28.82353 47 GTCTTCT 170 0.0 28.82353 49 ATCTCGT 160 1.8189894E-12 28.4375 37 ATGCCGT 185 0.0 28.378378 44 TAAGATC 150 2.7284841E-11 28.0 33 GCCGTCT 175 0.0 28.0 46 TCTTCTG 175 0.0 28.0 50 GTGGGGA 50 0.00712583 27.999998 4 TGCCGTC 190 0.0 27.631578 45 AGATCTC 165 3.6379788E-12 27.575758 35 >>END_MODULE