FastQCFastQC Report
Wed 25 May 2016
SRR1780725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492138
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT4940.10037834916222686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC207.895728E-443.95050442
AGCGTGA207.907592E-443.9371078
TATTGCG250.002349171635.2069211
ATTCCCT250.002362103235.16755327
CGTTCGG756.002665E-1132.2927324
TTCGGTA756.184564E-1132.2369226
CGACGTT551.3515273E-732.0519614
CATCGTA701.024091E-931.45395724
ATCGTAC701.033186E-931.42515425
TCGTACT701.0422809E-931.399626
TCGTTCG801.2914825E-1030.2651923
ACGTTAC602.8875547E-729.39294616
CGTTACT602.8875547E-729.39294617
CCGACGT602.9056537E-729.37198313
ACATCGT752.1482265E-929.3480623
CGACACG602.9290823E-729.34507236
GGCTTAT300.005764814629.30927329
TTAATAC300.00577915229.294382
CCGGAAT602.9764124E-729.2914053
CGGTAAG852.6557245E-1028.44434428
Produced by FastQC (version 0.11.5)