FastQCFastQC Report
Wed 25 May 2016
SRR1780725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492138
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT140722.8593605858519355No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT50021.0163815840272443TruSeq Adapter, Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC22970.4667390041004759No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11420.23204873429810338No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG10630.2159963262336987No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT5810.118056317536951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC404.118665E-738.4993844
GCGAACT750.038.1288428
GCTTCTA357.28761E-637.70984323
CCGTCTT5250.036.87559544
AAAGCGA800.035.7457925
CAGGTAC250.002346941735.2137411
TTGTATC250.002352800735.19585433
CCATTGA250.002352800735.19585429
CGAACTA850.033.64309729
TAGGGCG551.3662248E-732.0124939
GCCGTCT6050.031.6358543
AGCGAAC1000.030.79637327
GGCCACG300.00572795429.347771
GAATACG454.053885E-529.34478811
AGAGTCT300.005730801329.34478615
TATCGTC300.005730801329.34478613
ACGTCTG300.005742229.33285742
GTAAACA300.005742229.33285736
ATCGGAA901.6370905E-1129.3298827
TATCGGA901.6370905E-1129.3298826