FastQCFastQC Report
Wed 25 May 2016
SRR1780724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2127.176709546377793RNA PCR Primer, Index 30 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC290.9817197020988491No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC250.8463100880162491No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT240.8124576844955992No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGTCTT190.6431956668923494No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT150.5077860528097494RNA PCR Primer, Index 30 (95% over 21bp)
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA130.4400812457684496No Hit
ACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCT130.4400812457684496RNA PCR Primer, Index 30 (95% over 24bp)
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG120.4062288422477996No Hit
CCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCGCGTCCCTTTCTC100.3385240352064997No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGTCTT100.3385240352064997No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC90.3046716316858497No Hit
CCTCACAGGTTTCTACTCCAAAGACCACATCATCGAAACCGCAAACATAT80.2708192281651997No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA70.23696682464454977No Hit
CCAAAGACCACATCATCGAAACCGCAAACATATCATACACATCTCCGAGC70.23696682464454977No Hit
CGATTTCTAGGATAGTCAGTAGGATTAGAATTGTGAAGATGATAAGTGTA60.2031144211238998No Hit
ACCATGAGCCCTACAAACAACTAACCTGCCACTAATAGTTATGTCATCCC60.2031144211238998No Hit
CATCGAAACCGCAAACATATCATACACATCTCCGAGCCCACGAGACATTG60.2031144211238998No Hit
TCCTAGACCTAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAG60.2031144211238998No Hit
GACCACATCATCGAAACCGCAAACATATCATACACATCTCCGAGCCCACG60.2031144211238998No Hit
CTTCAAAGCCATACTATTTATGTGCTCCGGATCCATCATCCACAACCTTA60.2031144211238998No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGTCTT60.2031144211238998No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT60.2031144211238998No Hit
CCCTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGC50.16926201760324985No Hit
CTATATCCCCCGCCCGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCT50.16926201760324985No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC50.16926201760324985No Hit
CATCATCGAAACCGCAAACATATCATACACATCTCCGAGCCCACGAGACA50.16926201760324985No Hit
CCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCAT50.16926201760324985No Hit
TCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGATCC50.16926201760324985No Hit
CTATTTATGTGCTCCGGATCCATCATCCACAACCTTAACAATGAACAAGA50.16926201760324985No Hit
TATATCCCCCGCCCGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCTA50.16926201760324985No Hit
GGTTTCTACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACAC50.16926201760324985No Hit
CTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAGGAAT40.13540961408259986No Hit
CGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAG40.13540961408259986No Hit
ATACCTTTCCTCACAGGTTTCTACTCCAAAGACCACATCATCGAAACCGC40.13540961408259986No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA40.13540961408259986No Hit
GACTAGTATGGCAATAGGCACAATATTGGCTAAGAGGGAGTGGGTGTTGA40.13540961408259986No Hit
CTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTGCTTGA40.13540961408259986RNA PCR Primer, Index 30 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGTCTT40.13540961408259986Illumina PCR Primer Index 1 (95% over 21bp)
ACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAA40.13540961408259986No Hit
CTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACC40.13540961408259986No Hit
CCTATATCCCCCGCCCGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGC40.13540961408259986No Hit
GTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAG40.13540961408259986No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT40.13540961408259986No Hit
TTTCTACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACAT40.13540961408259986No Hit
CTACTAGGGTGTAGAATAGGAAGTATGTGCCTGCGTTCAGGCGTTCTGGC40.13540961408259986No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGCTCTCGTATGCCGTCTT40.13540961408259986No Hit
ATACACATCTCCGAGCCCACGAGCCATTGCCGGATCTCGTATGCCGTCTT30.1015572105619499RNA PCR Primer, Index 30 (95% over 22bp)
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT30.1015572105619499No Hit
GCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAG30.1015572105619499No Hit
CCTTTCCTCACAGGTTTCTACTCCAAAGACCACATCATCGAAACCGCAAA30.1015572105619499No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC30.1015572105619499No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGTCTT30.1015572105619499No Hit
GATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTC30.1015572105619499No Hit
ATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTACA30.1015572105619499No Hit
CGGCTTACTTCTCTTCATTCTCTCCTTAATGACATTAACACTATTCTCAC30.1015572105619499No Hit
CAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTATCACGAAAA30.1015572105619499No Hit
GTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATAT30.1015572105619499No Hit
TCCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGG30.1015572105619499No Hit
ACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATT30.1015572105619499No Hit
ACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCA30.1015572105619499No Hit
CTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT30.1015572105619499RNA PCR Primer, Index 30 (95% over 22bp)
ATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACTACT30.1015572105619499No Hit
TCATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTTT30.1015572105619499No Hit
TGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATCCTATA30.1015572105619499No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC30.1015572105619499No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC30.1015572105619499No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT30.1015572105619499No Hit
TCGCTAACCCCACTAAAACACTCACCAAGACCTCAACCCCTGACCCCCAT30.1015572105619499No Hit
TTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGA30.1015572105619499No Hit
ACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACATCTCCG30.1015572105619499No Hit
ATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTC30.1015572105619499No Hit
GTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT30.1015572105619499No Hit
GTTCTGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGC30.1015572105619499No Hit
CCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACATCTCC30.1015572105619499No Hit
TTGTATGACTATGGGCGTTGATTAGTAGTAGTTACTGGTTGAACATTGTT30.1015572105619499No Hit
GCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC30.1015572105619499No Hit
CCATCATTCCCCCTAAATAAATTAAAAAAACTATTAAACCCATATAACCT30.1015572105619499No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG30.1015572105619499No Hit
CCTATATCCCCTTTTATACTAATAATCTTATAGAAATTTAGGTTAAATAC30.1015572105619499No Hit
ATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTGCTT30.1015572105619499RNA PCR Primer, Index 18 (96% over 27bp)
CTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAGT30.1015572105619499No Hit
CCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACTACTCAAAACCA30.1015572105619499No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC30.1015572105619499No Hit
ACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAA30.1015572105619499No Hit
CTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCT30.1015572105619499No Hit
GACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCT30.1015572105619499No Hit
CATCAAGCCCGAATGATATTTCCTATTCGCATACACATCTCCGAGCCCAC30.1015572105619499No Hit
CTTGGGTAACCTCTGGGACTCAGAAGTGAAAGGGGGCTATTCCTAGTTTT30.1015572105619499No Hit
ATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGC30.1015572105619499No Hit
CCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACATCT30.1015572105619499No Hit
CCACTACTCCCACCCCCATACCCTTGGACCAAAAGCCAATACAACCATAT30.1015572105619499No Hit
AGTATATCCAAAGACAACCATCATTCCCCCTAAATAAATTAAAAAAACTA30.1015572105619499No Hit
GTGTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGTTGACTTG30.1015572105619499No Hit
CCCTAGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTATACA30.1015572105619499No Hit
AACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATT30.1015572105619499No Hit
CACGGGAGGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAG30.1015572105619499No Hit
TCTTCATTCTCTCCTTAATGACATTAACACTATTCTCACCAGACCTCCTA30.1015572105619499No Hit
CAGGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGA30.1015572105619499No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC30.1015572105619499No Hit
ATTTATGTGCTCCGGATCCATCATCCACAACCTTAACAATGAACAAGATA30.1015572105619499No Hit
AGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCCAAAGA30.1015572105619499No Hit
ACCTGACTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAG30.1015572105619499No Hit
ATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAA30.1015572105619499No Hit
GTGTAGGAAGCTGAATAATTTATGAAGGAGAGGGGTCAGGGTTGATTCGG30.1015572105619499No Hit
ATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG30.1015572105619499No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC30.1015572105619499No Hit
CTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAA30.1015572105619499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC403.5711862E-543.7500043
TTATACA403.5711862E-543.7500042
CTTATAC403.5711862E-543.7500041
ATCTCGT501.332663E-435.034
CGGATCT501.332663E-435.031
TCTCGTA501.332663E-435.035
GATCTCG501.332663E-435.033
GTCTTCT501.332663E-435.046
CGTCTTC501.332663E-435.045
TGCCGTC552.3350718E-431.81818442
TATGCCG552.3350718E-431.81818440
GGATCTC552.3350718E-431.81818432
TTCTGCT552.3350718E-431.81818449
CCGTCTT552.3350718E-431.81818444
TCTGCTT552.3350718E-431.81818450
ATGCCGT552.3350718E-431.81818441
CTGCTTG552.3350718E-431.81818451
GCCGTCT552.3350718E-431.81818443
TCTTCTG552.3350718E-431.81818447
CTCGTAT552.3350718E-431.81818436