FastQCFastQC Report
Wed 25 May 2016
SRR1780723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132411
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT3430.259041922498886No Hit
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT2960.22354638209816402No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATC2710.20466577550203535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA207.775974E-444.06323614
GCCTTAC207.8196026E-444.01323725
TCTCAAG254.4234574E-543.99659312
AGAGTAC207.8634254E-443.96335629
AAACTCG254.4432563E-543.96335227
CCTAACT207.878076E-443.94675441
TTGATAT301.286837E-436.70546322
GAAACTC301.3012456E-436.6361326
TGCTCCC301.3041432E-436.6222931
GCTCCCA301.3041432E-436.6222932
ACAAGCC250.002315812735.29067220
ACTTCGA250.002341787335.21058734
ACCATTC250.002346138735.19727712
CTCGCTA250.002346138735.19727713
CAGAGTA250.002354860835.17068528
TACTTCG250.002359231535.15740233
TGCTTAC250.002359231535.1574027
TAGCAAG250.002359231535.1574025
TGTTAAG250.002359231535.1574022
CCTTTAG803.6379788E-1232.9600687
Produced by FastQC (version 0.11.5)