FastQCFastQC Report
Wed 25 May 2016
SRR1780723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132411
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT1833813.84930255039234No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT79626.013095588735075TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC23531.777042692827635No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG18691.411514149126583No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC7860.5936062713822869No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC6570.49618234134626277No Hit
TATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCT6040.4561554553624699No Hit
ATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTTCTGCTT3360.25375535265196997TruSeq Adapter, Index 2 (96% over 27bp)
CATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTTCTGCT3300.24922400706889913TruSeq Adapter, Index 2 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTTCT3210.24242698869429277RNA PCR Primer, Index 2 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC3200.2416717644304476No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG3170.23940609163891216No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTA3040.22958817620892524No Hit
CTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGTG2600.19635830859973868No Hit
CGATTTCTAGGATAGTCAGTAGGATTAGAATTGTGAAGATGATAAGTGTA1780.13442991896443648No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC1770.13367469470059135No Hit
ACCATGAGCCCTACAAACAACTAACCTGCCACTAATAGTTATGTCATCCC1710.12914334911752046No Hit
GGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGC1640.12385677927060441No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC1610.12159110647906896No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC1380.10422094841063052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAATT207.819318E-444.01360314
CCGTGCG207.819318E-444.0136039
CGTAGCG207.819318E-444.01360315
TTGGTCG207.819318E-444.01360310
TCTTTAT502.7284841E-1144.01361
CGGAATC207.833887E-443.9969820
CGGCGAG207.833887E-443.9969833
CCCGTGC207.833887E-443.996988
TAGATGC207.833887E-443.996982
GCGGAAT207.833887E-443.9969819
CTTTATA502.7284841E-1143.996982
AATCGGG207.833887E-443.9969823
TGGCGTG900.041.552723
ATGATTC850.041.4089240
TGATTCA850.041.4089241
TTGAGGC950.039.3805911
GAGGCGC950.039.3805913
GGCGTGA950.039.3657224
GCCATTG950.039.3657218
ATTGGCG950.039.3657221
Produced by FastQC (version 0.11.5)