FastQCFastQC Report
Wed 25 May 2016
SRR1780722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13628
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA140.1027296742001761No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTTT150.002178709769.99264544
TATACAA252.3089319E-455.994122
GTTTATA252.3089319E-455.994122
AACGTTG200.00680880252.49448824
CCAACGT200.00680880252.49448822
GGCTTTG200.00680880252.49448845
ATACAAC200.00680880252.4944883
Produced by FastQC (version 0.11.5)