FastQCFastQC Report
Wed 25 May 2016
SRR1780722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13628
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3412.502201350161432Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT800.587026709715292No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC500.36689169357205753Illumina PCR Primer Index 10 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC400.293513354857646No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT180.13208100968594072Illumina PCR Primer Index 10 (95% over 22bp)
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG170.12474317581449956No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC170.12474317581449956Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCT160.1174053419430584Illumina PCR Primer Index 10 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGTCTT150.11006750807161726No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCATT150.002177802670.041
TATGTCT200.00680597552.549
CTCCATA200.00680597552.52
GTATAGG200.00680597552.51
TGCCGTC400.00233662635.042
CCGTCTT400.00233662635.044
CTGCTTG400.00233662635.051
GCCGTCT400.00233662635.043
CGTCTTC400.00233662635.045
GTATGCC450.00416187731.11110939
TATGCCG450.00416187731.11110940
ATGCCGT450.00416187731.11110941
TCTTCTG450.00416187731.11110947
CTTCTGC450.00416187731.11110948
TTCTGCT500.006966518727.99999849
TCTGCTT500.006966518727.99999850
TCTCGTA500.006966518727.99999835
GTCTTCT500.006966518727.99999846
CGTATGC500.006966518727.99999838
TCGTATG500.006966518727.99999837