Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780722_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13628 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT | 341 | 2.502201350161432 | Illumina PCR Primer Index 10 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT | 80 | 0.587026709715292 | No Hit |
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC | 50 | 0.36689169357205753 | Illumina PCR Primer Index 10 (95% over 21bp) |
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC | 40 | 0.293513354857646 | No Hit |
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT | 18 | 0.13208100968594072 | Illumina PCR Primer Index 10 (95% over 22bp) |
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG | 17 | 0.12474317581449956 | No Hit |
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC | 17 | 0.12474317581449956 | Illumina PCR Primer Index 10 (95% over 21bp) |
ACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCT | 16 | 0.1174053419430584 | Illumina PCR Primer Index 10 (96% over 25bp) |
ATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGTCTT | 15 | 0.11006750807161726 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCCCATT | 15 | 0.0021778026 | 70.0 | 41 |
TATGTCT | 20 | 0.006805975 | 52.5 | 49 |
CTCCATA | 20 | 0.006805975 | 52.5 | 2 |
GTATAGG | 20 | 0.006805975 | 52.5 | 1 |
TGCCGTC | 40 | 0.002336626 | 35.0 | 42 |
CCGTCTT | 40 | 0.002336626 | 35.0 | 44 |
CTGCTTG | 40 | 0.002336626 | 35.0 | 51 |
GCCGTCT | 40 | 0.002336626 | 35.0 | 43 |
CGTCTTC | 40 | 0.002336626 | 35.0 | 45 |
GTATGCC | 45 | 0.004161877 | 31.111109 | 39 |
TATGCCG | 45 | 0.004161877 | 31.111109 | 40 |
ATGCCGT | 45 | 0.004161877 | 31.111109 | 41 |
TCTTCTG | 45 | 0.004161877 | 31.111109 | 47 |
CTTCTGC | 45 | 0.004161877 | 31.111109 | 48 |
TTCTGCT | 50 | 0.0069665187 | 27.999998 | 49 |
TCTGCTT | 50 | 0.0069665187 | 27.999998 | 50 |
TCTCGTA | 50 | 0.0069665187 | 27.999998 | 35 |
GTCTTCT | 50 | 0.0069665187 | 27.999998 | 46 |
CGTATGC | 50 | 0.0069665187 | 27.999998 | 38 |
TCGTATG | 50 | 0.0069665187 | 27.999998 | 37 |