##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780722_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13628 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.975931904901675 34.0 33.0 34.0 27.0 34.0 2 32.793806868212506 34.0 34.0 34.0 31.0 34.0 3 33.09957440563546 34.0 34.0 34.0 32.0 34.0 4 32.987158790724976 34.0 34.0 34.0 31.0 34.0 5 33.02076606985618 34.0 34.0 34.0 31.0 34.0 6 36.635676548282945 38.0 37.0 38.0 34.0 38.0 7 36.34120927502202 38.0 37.0 38.0 34.0 38.0 8 36.13875843850895 38.0 37.0 38.0 32.0 38.0 9 36.07036982682712 38.0 37.0 38.0 32.0 38.0 10-11 35.52582917522747 37.5 35.5 38.0 29.5 38.0 12-13 35.448304960375694 37.0 36.0 38.0 31.0 38.0 14-15 35.756640739653655 37.5 36.0 38.0 31.5 38.0 16-17 35.92742882301145 38.0 36.5 38.0 32.0 38.0 18-19 35.90361755209862 38.0 36.0 38.0 32.0 38.0 20-21 35.619166422072205 38.0 36.0 38.0 31.0 38.0 22-23 35.55587760493103 37.5 35.5 38.0 29.0 38.0 24-25 34.72031846199002 37.0 34.5 38.0 27.5 38.0 26-27 35.00642060463751 37.0 35.0 38.0 29.0 38.0 28-29 34.83049603756971 37.0 35.0 38.0 27.0 38.0 30-31 34.314426181391255 37.0 34.0 38.0 25.5 38.0 32-33 34.89092309950102 37.0 35.0 38.0 27.0 38.0 34-35 34.81930584091576 37.0 34.5 38.0 27.0 38.0 36-37 35.09608893454652 37.0 36.0 38.0 27.0 38.0 38-39 34.87830202524215 37.0 35.0 38.0 28.5 38.0 40-41 34.08530231875551 37.0 34.0 38.0 25.0 38.0 42-43 34.63296154975052 37.0 34.5 38.0 26.5 38.0 44-45 34.95124009392427 37.0 35.0 38.0 29.0 38.0 46-47 35.09803346052246 37.0 35.5 38.0 27.0 38.0 48-49 34.743909597886706 37.0 34.5 38.0 27.0 38.0 50-51 35.07708394481949 37.0 35.5 38.0 27.5 38.0 52-53 34.964705019078366 37.0 35.0 38.0 27.0 38.0 54-55 35.048246257704726 37.0 35.0 38.0 27.0 38.0 56-57 35.25245817434693 37.0 36.0 38.0 29.0 38.0 58-59 35.35214264749046 38.0 36.0 38.0 30.5 38.0 60-61 34.83277076606986 37.0 35.0 38.0 28.0 38.0 62-63 35.20630319929557 37.0 35.5 38.0 30.0 38.0 64-65 35.205789550924564 37.0 36.0 38.0 29.0 38.0 66-67 34.365387437628414 37.0 34.0 38.0 25.5 38.0 68-69 33.43960962723804 37.0 34.0 38.0 22.5 38.0 70-71 33.21877751687702 37.0 34.0 38.0 22.0 38.0 72-73 32.75055033754036 37.0 31.5 38.0 20.0 38.0 74-75 33.609590548869974 37.0 34.0 38.0 23.5 38.0 76 33.12951276783094 37.0 33.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6767366215840696 1101 2 0.36455791138974547 1101 3 0.47134568036405255 1101 4 0.9429421968892342 1101 5 0.7699718489799778 1101 6 -0.3786506435722714 1101 7 0.01855810302449612 1101 8 0.5474780505057808 1101 9 1.196009813062588 1101 10-11 0.17862735969116272 1101 12-13 0.07576004189120766 1101 14-15 0.4465011146794282 1101 16-17 0.11620668866765982 1101 18-19 -0.7284051439918429 1101 20-21 -0.7322328417442492 1101 22-23 0.6630521295428409 1101 24-25 -1.983810907200514 1101 26-27 -0.0449019229460248 1101 28-29 1.090379609428581 1101 30-31 1.2095356516456235 1101 32-33 0.3836603873817239 1101 34-35 -0.4253587311584468 1101 36-37 0.994419955546185 1101 38-39 0.13454273665139738 1101 40-41 1.4674426103758762 1101 42-43 0.9395205406458942 1101 44-45 0.8988703485062359 1101 46-47 0.8228124357457887 1101 48-49 -0.6812680154168618 1101 50-51 -1.6414384201475087 1101 52-53 -1.8278885911756717 1101 54-55 -0.841397808698396 1101 56-57 -2.4295519299273707 1101 58-59 -1.7534861392967755 1101 60-61 -1.7452349314595708 1101 62-63 -0.30594656917509866 1101 64-65 -1.1702693352996292 1101 66-67 -2.9263468784326676 1101 68-69 -2.4417109244971087 1101 70-71 -1.0443553900637 1101 72-73 -3.0742439983204832 1101 74-75 -2.4737168584170135 1101 76 -3.832665267793047 1102 1 -2.036125267357555 1102 2 0.5539266821538646 1102 3 0.399679804711468 1102 4 0.8693778874445215 1102 5 0.6062319344095002 1102 6 0.572464686280604 1102 7 0.6165647475427676 1102 8 1.5109332000074431 1102 9 0.7475048296738862 1102 10-11 1.0492965576028865 1102 12-13 1.0686409151707537 1102 14-15 0.9953857848265599 1102 16-17 0.26926791315745646 1102 18-19 -1.5321545507312848 1102 20-21 0.9172688193853205 1102 22-23 0.7527531262205898 1102 24-25 -4.673891590636682 1102 26-27 -1.0339859286413287 1102 28-29 0.4525058552757599 1102 30-31 2.4219229321392177 1102 32-33 0.971937558715382 1102 34-35 0.5950494321068547 1102 36-37 -3.7276967981320297 1102 38-39 -1.2418217626366896 1102 40-41 -0.22240076883627324 1102 42-43 0.9919647741532458 1102 44-45 0.7569624225451577 1102 46-47 1.9229548182801963 1102 48-49 -3.1879125336892855 1102 50-51 -0.28382095455662437 1102 52-53 -0.12285774162654661 1102 54-55 0.27132169770881376 1102 56-57 1.5059013211404988 1102 58-59 -0.5494045066437181 1102 60-61 -6.629430470140161 1102 62-63 -2.732382259730393 1102 64-65 0.6273101616154193 1102 66-67 0.6583707295407493 1102 68-69 1.068255852911534 1102 70-71 -0.8291204588819241 1102 72-73 -6.546716708334721 1102 74-75 -2.492226587890201 1102 76 1.0016963838443544 1103 1 1.7075952977915776 1103 2 0.9495310777582588 1103 3 0.37966410612434487 1103 4 1.002423413347195 1103 5 0.682762546654395 1103 6 0.6195604476620815 1103 7 1.748432879410899 1103 8 1.851592540666779 1103 9 0.7062960384650907 1103 10-11 1.154477091354849 1103 12-13 0.10415896854594564 1103 14-15 -0.2247868996318374 1103 16-17 0.56812976559074 1103 18-19 1.2172567522514512 1103 20-21 0.5271589292754228 1103 22-23 0.46291796138542196 1103 24-25 -3.0938287962881716 1103 26-27 -0.04870335407304083 1103 28-29 0.8137695915394971 1103 30-31 1.8414676731125255 1103 32-33 1.5949752353244833 1103 34-35 0.5265643457646334 1103 36-37 -0.3483796866720681 1103 38-39 0.6698736847730444 1103 40-41 0.0067986032202398405 1103 42-43 0.36460998608417583 1103 44-45 2.025212030080482 1103 46-47 1.8393598418280774 1103 48-49 -2.5464290172057673 1103 50-51 0.32077088217807415 1103 52-53 1.3369146288601073 1103 54-55 0.8996576474733331 1103 56-57 1.4022906461012496 1103 58-59 -0.43617844698908215 1103 60-61 -4.09116516401771 1103 62-63 -1.5288893240945995 1103 64-65 -0.13426754639086624 1103 66-67 -0.06140949023946973 1103 68-69 1.0531459628016435 1103 70-71 1.4762170607413125 1103 72-73 -5.900719848052145 1103 74-75 -2.1729565721916124 1103 76 -3.134096394805571 1104 1 -0.15125811294862146 1104 2 0.4488053883533212 1104 3 0.4489674442956044 1104 4 -0.20185815414192376 1104 5 -0.8030865099493809 1104 6 0.8793572546286939 1104 7 1.1852979011815776 1104 8 1.3842485638888462 1104 9 1.3162379833126963 1104 10-11 -1.111850920256174 1104 12-13 0.7157337145546521 1104 14-15 1.0092479334595978 1104 16-17 0.5879048018752115 1104 18-19 0.5802828770700259 1104 20-21 -0.5679063827710138 1104 22-23 0.6125914011532032 1104 24-25 1.3176370963097952 1104 26-27 2.0033648607310255 1104 28-29 1.9942848310169978 1104 30-31 0.38900032913575444 1104 32-33 -0.0840886253431492 1104 34-35 0.31049032544532906 1104 36-37 1.567258920004285 1104 38-39 2.152094294352146 1104 40-41 1.2157971518414357 1104 42-43 0.800205051396226 1104 44-45 1.6298927267423053 1104 46-47 1.8418997547453486 1104 48-49 0.578933827496698 1104 50-51 0.7995444670837344 1104 52-53 0.676718692720776 1104 54-55 1.3731699841932183 1104 56-57 0.4269640858690309 1104 58-59 -0.41386349778733944 1104 60-61 0.9206272888124758 1104 62-63 0.5558349139315197 1104 64-65 1.40147265680217 1104 66-67 1.2041928319666297 1104 68-69 1.5736466884910456 1104 70-71 1.8659122711912346 1104 72-73 0.6348389327896555 1104 74-75 0.5354977093758748 1104 76 -1.2262589492322746 1105 1 1.0455073857036652 1105 2 0.8217838250110034 1105 3 0.7134553149155494 1105 4 0.9076431935669689 1105 5 -0.567237453345605 1105 6 0.5316483597499939 1105 7 1.0808504618284829 1105 8 -0.6676382285639875 1105 9 0.9975048296738862 1105 10-11 0.7355210473988052 1105 12-13 -0.11630806442108366 1105 14-15 0.6165592542143159 1105 16-17 -0.24986473990377078 1105 18-19 0.5558556533503562 1105 20-21 0.11369739081388985 1105 22-23 0.14561026907772856 1105 24-25 0.9549349399755656 1105 26-27 0.7019834591137766 1105 28-29 1.6935772838471905 1105 30-31 2.0749841566290073 1105 32-33 1.3724477627970089 1105 34-35 -0.8539301597298774 1105 36-37 0.6090378957455229 1105 38-39 0.31685170675106633 1105 40-41 0.8515788230004588 1105 42-43 0.7725770190512051 1105 44-45 1.7965032388716935 1105 46-47 1.5900466550148948 1105 48-49 -4.1253368928551026E-4 1105 50-51 1.1779137393209353 1105 52-53 1.6322442991897788 1105 54-55 1.3172400650557492 1105 56-57 0.808197239507841 1105 58-59 1.487585289274655 1105 60-61 1.3909776931251514 1105 62-63 -0.529576137281417 1105 64-65 1.2421060799827615 1105 66-67 0.06908501525504107 1105 68-69 0.3361129957686728 1105 70-71 0.9514917860160352 1105 72-73 1.5285383937061 1105 74-75 0.08047749374244972 1105 76 1.3920025062933306 1106 1 -0.9671910269947439 1106 2 0.8777362059633873 1106 3 -0.03614785968762391 1106 4 -0.0022774413536623683 1106 5 0.6660958799877292 1106 6 0.5221245502261809 1106 7 -0.7199431889651677 1106 8 1.3934728825471225 1106 9 -0.47789199572294194 1106 10-11 1.3279813648591272 1106 12-13 1.93984272922971 1106 14-15 -0.8098296346745784 1106 16-17 0.5606511331121027 1106 18-19 0.5131969231916216 1106 20-21 0.03314183525833414 1106 22-23 0.6956102690777257 1106 24-25 -0.3528031552625279 1106 26-27 1.7998009194312345 1106 28-29 1.4477439505138534 1106 30-31 1.6495873312321905 1106 32-33 0.7712572866065415 1106 34-35 1.1190857132859975 1106 36-37 1.8804664671740952 1106 38-39 0.5831215480209053 1106 40-41 1.717055013476653 1106 42-43 2.0844817809559757 1106 44-45 1.4066222864907303 1106 46-47 1.11841967088791 1106 48-49 1.9089128631361163 1106 50-51 -0.5153402289330344 1106 52-53 1.035418902364384 1106 54-55 0.018033715849405496 1106 56-57 1.1988718426824505 1106 58-59 1.6687360829254487 1106 60-61 1.3893903915378445 1106 62-63 1.5503841801789022 1106 64-65 -0.9733701104934269 1106 66-67 1.1266246977947247 1106 68-69 2.3952399798956634 1106 70-71 0.496729881254133 1106 72-73 1.5727844254521273 1106 74-75 1.064009239774201 1106 76 0.1368437761346044 1107 1 1.1691337593300446 1107 2 0.6226080008351786 1107 3 0.11043333689357127 1107 4 0.6370387979625747 1107 5 0.37507023896208835 1107 6 0.5426373707390013 1107 7 1.075355956333972 1107 8 0.9669771560513993 1107 9 0.5332191153881709 1107 10-11 0.866015552893316 1107 12-13 0.40223589162287254 1107 14-15 0.3521361772912357 1107 16-17 0.9335143809753532 1107 18-19 0.9480259830206776 1107 20-21 0.7963896985061965 1107 22-23 1.1167641152315753 1107 24-25 0.47347889601952176 1107 26-27 0.14360433823465257 1107 28-29 1.0445388223087235 1107 30-31 0.8991599808048392 1107 32-33 1.1526675430167899 1107 34-35 1.0457951149954 1107 36-37 1.7093126210202456 1107 38-39 0.5352583001576576 1107 40-41 -0.16627831985667996 1107 42-43 1.2107638322380225 1107 44-45 0.3617504916189418 1107 46-47 1.4355136879819241 1107 48-49 1.357417136640386 1107 50-51 1.9169247283319208 1107 52-53 1.5676838596293408 1107 54-55 1.1208114936271798 1107 56-57 1.4119060307166365 1107 58-59 0.16959078378014425 1107 60-61 1.9280656052130567 1107 62-63 1.1730337528284807 1107 64-65 1.0772709151476008 1107 66-67 0.03474435591438407 1107 68-69 2.158915193570877 1107 70-71 1.0531401376643856 1107 72-73 1.214664767332465 1107 74-75 0.048197273962230724 1107 76 1.4812882205790459 1108 1 -0.3916354714391872 1108 2 0.7910695392967213 1108 3 0.09274102920126381 1108 4 0.8555003364241145 1108 5 -0.3472374533456062 1108 6 0.26879121689285057 1108 7 -0.03772096674294545 1108 8 0.1423617714360148 1108 9 0.39321911538817034 1108 10-11 0.8190924759702369 1108 12-13 1.057620507007485 1108 14-15 0.7690592542143193 1108 16-17 -0.7137933113323385 1108 18-19 0.9176413676360582 1108 20-21 1.3886973908138884 1108 22-23 0.15561026907773368 1108 24-25 0.8238635114041344 1108 26-27 -0.7090879694576557 1108 28-29 -0.7289227161528089 1108 30-31 1.0706984423433 1108 32-33 1.364590619939868 1108 34-35 1.4646412688415538 1108 36-37 1.4304664671740923 1108 38-39 1.0275659924653482 1108 40-41 2.043721680143321 1108 42-43 0.4311484476226397 1108 44-45 0.643288953157402 1108 46-47 0.8339752264434637 1108 48-49 1.1878017520250026 1108 50-51 1.1157708821780759 1108 52-53 -0.5534699865245045 1108 54-55 -0.5341885063728213 1108 56-57 0.45998295379355625 1108 58-59 -0.26348613929677356 1108 60-61 1.4038348359822876 1108 62-63 1.4614952912900137 1108 64-65 1.2399632228399113 1108 66-67 1.5355135866836065 1108 68-69 1.5896844243401063 1108 70-71 0.180063214587463 1108 72-73 0.8738955365632393 1108 74-75 -0.8148796491146868 1108 76 2.4012882205790476 1109 1 -0.3116354714391889 1109 2 0.6610695392967187 1109 3 0.8027410292012647 1109 4 1.061863972787748 1109 5 -0.1808738169819719 1109 6 0.04788212598375452 1109 7 -0.6886300576520412 1109 8 0.7187254077996457 1109 9 1.1241282062972644 1109 10-11 0.8345470214247825 1109 12-13 1.1312568706438455 1109 14-15 0.7926956178506757 1109 16-17 1.1389339613949332 1109 18-19 1.5144595494542514 1109 20-21 0.55687920899571 1109 22-23 -1.0589351854677247 1109 24-25 0.1343180568586817 1109 26-27 0.710912030542346 1109 28-29 -0.2893772616073562 1109 30-31 1.417516624161479 1109 32-33 1.411408801758057 1109 34-35 1.4723685415688195 1109 36-37 2.1350119217195456 1109 38-39 1.659384174283531 1109 40-41 0.6519034983251402 1109 42-43 0.7693302658044558 1109 44-45 1.459652589521042 1109 46-47 1.212611590079824 1109 48-49 0.5978017520249992 1109 50-51 0.5594072458144339 1109 52-53 0.7783481952936739 1109 54-55 -0.061006688191007186 1109 56-57 0.5963465901571965 1109 58-59 0.837422951612318 1109 60-61 1.329289381436837 1109 62-63 1.0183134731082006 1109 64-65 0.16905413193081387 1109 66-67 0.6632408594108838 1109 68-69 0.1965026061582833 1109 70-71 0.9027904873147321 1109 72-73 0.6657137183814257 1109 74-75 -0.1930614672965092 1109 76 -0.04143905214822752 1110 1 -0.4013790611827801 1110 2 -0.050468922241741154 1110 3 -0.46007948361924633 1110 4 -0.18347402255024292 1110 5 -0.16980155590970725 1110 6 -0.9189010907994586 1110 7 0.6458687768467968 1110 8 0.18492587400011473 1110 9 1.1614242435933022 1110 10-11 -0.858343421465662 1110 12-13 0.44069743008440554 1110 14-15 1.0380336131886736 1110 16-17 0.5649246373856087 1110 18-19 -0.15774324774854875 1110 20-21 1.02715892927543 1110 22-23 -0.18131280784535164 1110 24-25 -1.1579313603907408 1110 26-27 1.0955274151577328 1110 28-29 -0.33687143410152487 1110 30-31 -1.5495579679131097 1110 32-33 0.18151369686295027 1110 34-35 0.1515643457646334 1110 36-37 2.1548254415330703 1110 38-39 1.3173095822089422 1110 40-41 -3.467560371138731 1110 42-43 0.07935357582776703 1110 44-45 0.9450838249522775 1110 46-47 1.5765393290075664 1110 48-49 2.2516479058711525 1110 50-51 -0.24012655371935665 1110 52-53 0.24396591091139186 1110 54-55 -0.2093167115010246 1110 56-57 0.004854748665351849 1110 58-59 -0.16374254955318435 1110 60-61 -0.3636010614536076 1110 62-63 -0.6538893240945995 1110 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2116 64-65 -1.906703443826764 2116 66-67 -1.3455975244275038 2116 68-69 -1.6658711312154502 2116 70-71 1.0189521034763516 2116 72-73 0.4561177587854601 2116 74-75 -0.9859907602258033 2116 76 -3.4076006683098434 2117 1 1.625864528560811 2117 2 -0.5889304607032813 2117 3 -0.38475897079873533 2117 4 0.6130003364241148 2117 5 -0.504737453345605 2117 6 0.888791216892848 2117 7 1.5897790332570523 2117 8 0.6448617714360125 2117 9 -1.154280884611829 2117 10-11 -0.8671575240297642 2117 12-13 -0.6073794929925143 2117 14-15 0.4603092542143159 2117 16-17 -1.0400433113323402 2117 18-19 0.17639136763606444 2117 20-21 0.5511973908138899 2117 22-23 0.7393602690777286 2117 24-25 -1.004886488595865 2117 26-27 -0.007837969457654026 2117 28-29 -1.2751727161528095 2117 30-31 -0.7330515576567009 2117 32-33 -1.05165938006013 2117 34-35 1.4496412688415532 2117 36-37 -2.4445335328259077 2117 38-39 1.633815992465351 2117 40-41 0.95872168014332 2117 42-43 -0.04885155237736427 2117 44-45 -0.08296104684259831 2117 46-47 -0.7760247735565358 2117 48-49 0.2540517520249992 2117 50-51 -1.5229791178219259 2117 52-53 1.252780013475494 2117 54-55 -0.2854385063728202 2117 56-57 0.8974829537935562 2117 58-59 -2.3472361392967755 2117 60-61 -0.9661651640177098 2117 62-63 -2.2260047087099863 2117 64-65 -0.24003677716009264 2117 66-67 -0.5157364133163895 2117 68-69 0.3896844243401034 2117 70-71 -0.9011867854125342 2117 72-73 0.7651455365632387 2117 74-75 -0.9373796491146891 2117 76 -0.018711779420954144 2118 1 0.1883645285608111 2118 2 1.1610695392967187 2118 3 0.42774102920126467 2118 4 1.1755003364241148 2118 5 0.932762546654395 2118 6 0.451291216892848 2118 7 1.7772790332570523 2118 8 2.0823617714360125 2118 9 0.2207191153881709 2118 10-11 0.8828424759702358 2118 12-13 0.01762050700748574 2118 14-15 1.835309254214316 2118 16-17 -0.6337933113323402 2118 18-19 1.3013913676360644 2118 20-21 1.6761973908138899 2118 22-23 1.8018602690777286 2118 24-25 1.088863511404135 2118 26-27 -1.851587969457654 2118 28-29 -1.6814227161528095 2118 30-31 0.32944844234329906 2118 32-33 -1.20790938006013 2118 34-35 0.2621412688415532 2118 36-37 -2.5382835328259077 2118 38-39 0.10256599246535103 2118 40-41 0.05247168014332004 2118 42-43 1.9511484476226357 2118 44-45 1.4795389531574017 2118 46-47 1.0989752264434642 2118 48-49 1.5353017520249992 2118 50-51 -1.2729791178219259 2118 52-53 -2.403469986524506 2118 54-55 -1.5041885063728202 2118 56-57 -0.008767046206443752 2118 58-59 0.49651386070322445 2118 60-61 0.5650848359822902 2118 62-63 -0.5385047087099863 2118 64-65 0.9787132228399074 2118 66-67 1.8280135866836105 2118 68-69 -0.3290655756598966 2118 70-71 2.755063214587466 2118 72-73 0.7338955365632387 2118 74-75 0.3126203508853109 2118 76 0.29378822057904586 2119 1 1.188364528560811 2119 2 -3.204315076087898 2119 3 0.20658718304741797 2119 4 -0.9783458174220385 2119 5 -0.29800668411483855 2119 6 -1.0727472446456119 2119 7 -1.0207978898198675 2119 8 0.08236177143601253 2119 9 -0.9667808846118291 2119 10-11 -1.4993690624912972 2119 12-13 -0.40545641606943406 2119 14-15 0.4867515619066225 2119 16-17 -1.1914856190246468 2119 18-19 -2.0038970939024026 2119 20-21 -1.7805333784168766 2119 22-23 -0.25823588476843184 2119 24-25 1.5984788960195218 2119 26-27 -0.4044725848422672 2119 28-29 2.150308053077964 2119 30-31 0.6299292115740727 2119 32-33 0.29689831224756347 2119 34-35 0.9207951149953999 2119 36-37 -1.9349181482105209 2119 38-39 -2.772434007534649 2119 40-41 -2.64704755062591 2119 42-43 -0.24115924468505767 2119 44-45 -0.4267110468425983 2119 46-47 1.320129072597311 2119 48-49 -0.2098905556673074 2119 50-51 1.8880785744857675 2119 52-53 -1.153469986524506 2119 54-55 -1.3118808140651268 2119 56-57 -3.943863200052597 2119 58-59 0.8234369376263047 2119 60-61 1.95691175905921 2119 62-63 -1.346197016402293 2119 64-65 0.18304014591682716 2119 66-67 1.784744355914384 2119 68-69 1.2550690397247166 2119 70-71 0.24544782997207903 2119 72-73 1.214664767332465 2119 74-75 -1.086418110653149 2119 76 1.2889805282713525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 2.0 23 7.0 24 18.0 25 18.0 26 36.0 27 78.0 28 165.0 29 252.0 30 467.0 31 712.0 32 1012.0 33 1377.0 34 1944.0 35 2424.0 36 3023.0 37 2092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.46257704725565 27.326093337246842 11.850601702377459 28.360727913120048 2 17.03111241561491 19.437921925447608 40.695626651012624 22.83533900792486 3 15.754329321984152 29.00645729380687 30.53272673906663 24.706486645142352 4 11.92398004109187 20.457880833577928 37.92192544760787 29.696213677722337 5 11.916642207220429 39.74170824772527 35.551805107132374 12.789844437921927 6 25.85118872908717 38.60434399765189 18.036395656002348 17.508071617258587 7 25.220135016143235 33.40915761667156 22.585852656295863 18.784854710889345 8 21.015556207807457 36.33695333137658 22.71059583211036 19.936894628705605 9 22.13090695626651 15.4241267977693 22.417082477252713 40.027883768711476 10-11 23.026122688582333 25.693425300851192 29.083504549457 22.19694746110948 12-13 24.614763721749338 22.725271499853246 26.262107425887876 26.39785735250954 14-15 22.02083944819489 26.430877604931023 23.033460522453773 28.51482242442031 16-17 21.826386850601704 27.696653947754623 24.944966245964192 25.531992955679485 18-19 20.967860287643088 28.32036982682712 25.554006457293806 25.157763428235985 20-21 21.87408277076607 27.7993836219548 24.86791899031406 25.458614616965068 22-23 22.923393014382153 28.441444085705896 26.416201937188145 22.218960962723806 24-25 21.73466392720869 29.898004109186964 26.14470208394482 22.222629879659525 26-27 21.67596125623716 29.54578808335779 26.115350748459054 22.662899911945996 28-29 20.523921338420898 29.26695039624303 28.88538303492809 21.323745230407983 30-31 22.58218373936014 29.369680070443206 26.46389785735251 21.584238332844144 32-33 20.832110361021428 28.50748459054887 29.637511006750806 21.022894041678896 34-35 20.611975344878193 29.454065159964777 29.666862342236573 20.267097152920456 36-37 21.000880540064575 26.86380980334605 28.86703845024949 23.26827120633989 38-39 21.576900498972705 27.59392427355445 26.91150572351042 23.91766950396243 40-41 20.703698268271207 27.443498679189904 28.980774875256827 22.87202817728207 42-43 22.97475785148224 26.46022894041679 29.200909891400055 21.364103316700913 44-45 20.13868506017024 27.949809216319345 27.245377164660994 24.66612855884943 46-47 22.673906662753154 25.961256237158793 28.610214264749047 22.75462283533901 48-49 20.890813031992955 26.207073671852072 28.595538597006165 24.30657469914881 50-51 20.560610507778105 25.924567067801586 29.050484297035517 24.464338127384796 52-53 21.393454652186676 26.324479013795127 30.011740534194303 22.270325799823894 54-55 19.041678896389787 26.70971529204579 30.162166128558848 24.086439683005576 56-57 21.81171118285882 25.961256237158793 28.96976812444966 23.257264455532727 58-59 19.38655708834752 29.931024361608454 28.019518638098035 22.662899911945996 60-61 19.70208394481949 31.127091282653364 27.069269151746404 22.101555620780747 62-63 17.85661872615204 32.49926621661285 26.632668036395657 23.011447020839448 64-65 19.008658643968303 32.51761080129146 25.509979454065164 22.963751100675083 66-67 19.27282066334018 31.824185500440272 25.50631053712944 23.39668329909011 68-69 19.26181391253302 31.45362488993249 25.81449955972997 23.47006163780452 70-71 18.979307308482536 30.37129439389492 25.88420898150866 24.765189316113883 72-73 19.27282066334018 29.68153800997945 25.788817141179926 25.25682418550044 74-75 19.621367772233636 30.004402700322863 25.92823598473731 24.445993542706194 76 19.7754622835339 30.158497211623132 25.38156736131494 24.68447314352803 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 2.0 20 1.5 21 0.0 22 3.0 23 9.5 24 15.5 25 18.0 26 27.5 27 44.5 28 65.5 29 79.0 30 88.0 31 129.5 32 193.5 33 225.0 34 244.0 35 346.5 36 442.5 37 455.0 38 560.0 39 778.0 40 966.0 41 1071.5 42 1102.0 43 1041.0 44 1050.0 45 1091.0 46 1062.0 47 1067.5 48 998.5 49 824.5 50 725.0 51 653.5 52 515.0 53 414.5 54 381.0 55 343.0 56 245.5 57 161.0 58 136.0 59 105.0 60 61.5 61 37.0 62 25.0 63 18.5 64 6.5 65 2.0 66 1.5 67 0.0 68 0.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.06780158497212 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73960612691467 51.46756677428823 2 13.216630196936544 17.72820663340182 3 5.229759299781182 10.52245377164661 4 2.3632385120350112 6.339888464925155 5 1.1378555798687089 3.8156736131493987 6 0.6564551422319475 2.6416201937188144 7 0.18599562363238512 0.8732022307014969 8 0.16411378555798686 0.8805400645729381 9 0.10940919037199125 0.6604050484297036 >10 0.175054704595186 1.9812151452891107 >50 0.010940919037199124 0.587026709715292 >100 0.010940919037199124 2.502201350161432 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 341 2.502201350161432 Illumina PCR Primer Index 10 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 80 0.587026709715292 No Hit CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 50 0.36689169357205753 Illumina PCR Primer Index 10 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 40 0.293513354857646 No Hit TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT 18 0.13208100968594072 Illumina PCR Primer Index 10 (95% over 22bp) CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG 17 0.12474317581449956 No Hit CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 17 0.12474317581449956 Illumina PCR Primer Index 10 (95% over 21bp) ACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCT 16 0.1174053419430584 Illumina PCR Primer Index 10 (96% over 25bp) ATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGTCTT 15 0.11006750807161726 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.00733783387144115 0.0 13 0.0 0.0 0.0 0.00733783387144115 0.0 14 0.0 0.0 0.0 0.00733783387144115 0.0 15 0.0 0.0 0.0 0.0146756677428823 0.0 16 0.0 0.0 0.0 0.02201350161432345 0.0 17 0.0 0.0 0.0 0.02201350161432345 0.0 18 0.0 0.0 0.0 0.02201350161432345 0.0 19 0.0 0.0 0.0 0.0440270032286469 0.0 20 0.0 0.0 0.0 0.0440270032286469 0.0 21 0.0 0.0 0.0 0.06604050484297036 0.0 22 0.0 0.0 0.0 0.08071617258585266 0.0 23 0.0 0.0 0.0 0.08071617258585266 0.0 24 0.0 0.0 0.0 0.08071617258585266 0.0 25 0.0 0.0 0.0 0.08071617258585266 0.0 26 0.0 0.0 0.0 0.08071617258585266 0.0 27 0.0 0.0 0.0 0.0880540064572938 0.0 28 0.0 0.0 0.0 0.0880540064572938 0.0 29 0.0 0.0 0.0 0.0880540064572938 0.0 30 0.0 0.0 0.0 0.0880540064572938 0.0 31 0.0 0.0 0.0 0.0880540064572938 0.0 32 0.0 0.0 0.0 0.1027296742001761 0.0 33 0.0 0.0 0.0 0.12474317581449956 0.0 34 0.0 0.0 0.0 0.15409451130026416 0.0 35 0.0 0.0 0.0 0.16877017904314645 0.0 36 0.0 0.0 0.0 0.22013501614323452 0.0 37 0.0 0.0 0.0 0.3302025242148518 0.0 38 0.0 0.0 0.0 0.3962430290578221 0.0 39 0.0 0.0 0.0 0.5429997064866452 0.0 40 0.0 0.0 0.0 0.7044320516583504 0.0 41 0.0 0.0 0.0 0.9319049016730261 0.0 42 0.0 0.0 0.0 1.1300264162019371 0.0 43 0.0 0.0 0.0 1.4969181097739948 0.0 44 0.0 0.0 0.0 1.7317287936601116 0.0 45 0.0 0.0 0.0 2.0252421485177576 0.0 46 0.0 0.0 0.0 2.201350161432345 0.0 47 0.0 0.0 0.0 2.634282359847373 0.0 48 0.0 0.0 0.0 2.920457880833578 0.0 49 0.0 0.0 0.0 3.221309069562665 0.0 50 0.0 0.0 0.0 3.4928089228059878 0.0 51 0.0 0.0 0.0 3.749633108306428 0.0 52 0.0 0.0 0.0 4.101849134135604 0.0 53 0.0 0.0 0.0 4.600821837393601 0.0 54 0.0 0.0 0.0 4.960375697094218 0.0 55 0.0 0.0 0.0 5.239213384208981 0.0 56 0.0 0.0 0.0 5.584091576166715 0.0 57 0.0 0.0 0.0 5.8702670971529205 0.0 58 0.0 0.0 0.0 6.200469621367772 0.0 59 0.0 0.0 0.0 6.626063985911359 0.0 60 0.0 0.0 0.0 6.853536835926034 0.0 61 0.0 0.0 0.0 7.13971235691224 0.0 62 0.0 0.0 0.0 7.374523040798357 0.0 63 0.0 0.0 0.0 7.763428235984738 0.0 64 0.0 0.0 0.0 8.15967126504256 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCATT 15 0.0021778026 70.0 41 TATGTCT 20 0.006805975 52.5 49 CTCCATA 20 0.006805975 52.5 2 GTATAGG 20 0.006805975 52.5 1 TGCCGTC 40 0.002336626 35.0 42 CCGTCTT 40 0.002336626 35.0 44 CTGCTTG 40 0.002336626 35.0 51 GCCGTCT 40 0.002336626 35.0 43 CGTCTTC 40 0.002336626 35.0 45 GTATGCC 45 0.004161877 31.111109 39 TATGCCG 45 0.004161877 31.111109 40 ATGCCGT 45 0.004161877 31.111109 41 TCTTCTG 45 0.004161877 31.111109 47 CTTCTGC 45 0.004161877 31.111109 48 TTCTGCT 50 0.0069665187 27.999998 49 TCTGCTT 50 0.0069665187 27.999998 50 TCTCGTA 50 0.0069665187 27.999998 35 GTCTTCT 50 0.0069665187 27.999998 46 CGTATGC 50 0.0069665187 27.999998 38 TCGTATG 50 0.0069665187 27.999998 37 >>END_MODULE