FastQCFastQC Report
Wed 25 May 2016
SRR1780721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492436
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT5710.11595415444849687No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT4930.10011453264992812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTT207.8484183E-444.0041722
TAGTGCC207.9075707E-443.9371386
GTAGCGA250.002350347135.20333522
TGCCGGT250.002362099535.16756425
CCGGTTC250.002364455535.16042327
GTACAGT451.0471977E-634.173333
CGTTACT401.7928189E-533.06702817
CTCGCTT353.1732718E-431.49882916
ATTGTCG353.1770684E-431.49240717
ATTCGAT502.3803605E-630.76224332
TTGCGAT300.00567941429.39890716
ACGTTAC454.0032282E-529.39890516
TGACCCG300.00573622929.33909610
CGCGAAG300.00574193529.333131
AAAGTAG300.00578200429.2914285
TACCGTT300.00578200429.2914287
CTTTAGT1350.029.2914268
CGGACGT554.926087E-628.01975436
CGGTAAG554.996742E-627.9685228
ATCAGTC406.9972523E-427.519438
Produced by FastQC (version 0.11.5)