FastQCFastQC Report
Wed 25 May 2016
SRR1780721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492436
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT158843.2255968288264874No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT59771.2137617883339156No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC20960.4256390678179499No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC14830.30115588624714684No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG12470.25323087670275934No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC6250.12692004646289062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCA207.8501285E-444.00223512
CGGCACA207.8501285E-444.00223514
GCACGAT207.8501285E-444.00223524
ACGATCT207.8501285E-444.00223526
GTGCCCG207.8501285E-444.0022359
GTCCTTC207.854056E-443.9977740
ATCTGCG207.854056E-443.9977729
CCGTCTT7100.035.94183744
TGAAGGT1850.035.67386228
GAACCGG250.00235085735.20178610
AGTCCCC250.002352028635.19821516
GGCACTA250.002352028635.19821531
TCCTTCG250.002352028635.19821541
ATTGGCG1950.034.97613521
CGCCATT2000.034.1017317
GCCGTCT7700.033.14117443
CACGTTT401.8172615E-533.00167512
ATGATTC2000.032.99832540
GAGGCGC2100.032.4778413
TGGCGTG2050.032.1967623
Produced by FastQC (version 0.11.5)