FastQCFastQC Report
Wed 25 May 2016
SRR1780720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6154
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGCAGTCCGGTGTAGATCTCGGTG70.11374715632109196No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG70.11374715632109196No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA651.7860675E-532.3076932
CTTATAC651.7860675E-532.3076931
TATACAC1008.85484E-931.5000023
ATACACA1102.242632E-828.6363624
ACACATC1451.1306838E-824.137936
TACACAT1351.6377817E-723.3333325
CCGACGA800.002380163221.87521
CACATCT1153.3302218E-521.3043487
Produced by FastQC (version 0.11.5)