##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780720_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6154 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.555736106597337 21.0 12.0 23.0 12.0 33.0 2 20.2708807279818 21.0 11.0 31.0 11.0 34.0 3 19.953526161845954 21.0 11.0 31.0 11.0 34.0 4 21.086935326616835 21.0 11.0 32.0 11.0 34.0 5 20.289080272993175 21.0 11.0 31.0 11.0 34.0 6 22.65290867728307 23.0 11.0 34.0 11.0 37.0 7 23.119109522261944 25.0 11.0 34.0 11.0 37.0 8 23.303867403314918 26.0 11.0 34.0 11.0 37.0 9 23.280630484237893 24.0 12.0 34.0 12.0 37.0 10-11 21.818817029574262 21.0 11.0 33.5 11.0 37.0 12-13 21.520393240168993 21.0 11.0 32.0 11.0 37.0 14-15 21.23635034124147 21.0 11.0 31.5 11.0 36.5 16-17 20.680045498862526 21.0 11.0 31.0 11.0 36.5 18-19 20.928989275268115 21.0 11.0 31.0 11.0 36.0 20-21 21.15924601884953 21.5 11.0 31.0 11.0 36.5 22-23 20.525430614234644 21.0 11.0 29.5 10.5 36.0 24-25 20.640315242118945 16.0 11.0 31.0 10.5 36.0 26-27 20.420051998700032 21.0 11.0 31.0 10.5 36.0 28-29 19.869515762105948 16.0 11.0 27.0 11.0 36.0 30-31 19.797530061748454 11.0 11.0 27.0 11.0 36.0 32-33 20.181589210269742 16.0 11.0 27.0 11.0 36.0 34-35 20.66135846603835 21.0 11.0 29.0 11.0 36.0 36-37 20.28574910627234 16.0 11.0 29.0 11.0 36.0 38-39 19.523886902827428 11.0 11.0 27.0 11.0 35.5 40-41 19.85505362365941 16.0 11.0 27.0 11.0 34.5 42-43 20.309554761130972 21.0 11.0 27.0 11.0 36.0 44-45 19.91030224244394 20.0 11.0 27.0 11.0 35.0 46-47 20.043955151121224 16.0 11.0 27.0 11.0 36.0 48-49 20.264787130321743 21.0 11.0 27.0 11.0 35.5 50-51 20.613990900227492 21.0 11.0 29.0 11.0 36.0 52-53 20.759831004224893 21.0 11.0 29.5 11.0 36.0 54-55 20.676145596360094 21.0 11.0 31.0 11.0 35.5 56-57 19.75763730906727 16.0 11.0 27.0 11.0 34.0 58-59 20.09904127396815 16.0 11.0 29.0 11.0 34.0 60-61 20.102453688657782 20.0 11.0 27.0 11.0 34.5 62-63 19.728306792330194 15.5 11.0 26.5 11.0 34.0 64-65 20.05110497237569 20.0 11.0 28.5 11.0 35.0 66-67 20.370165745856355 19.5 11.0 29.0 11.0 35.0 68-69 19.850341241468964 19.0 11.0 27.5 11.0 34.5 70-71 19.720344491387714 19.0 11.0 26.5 11.0 34.0 72-73 19.898115047123824 19.0 11.0 27.0 11.0 34.0 74-75 19.370409489762757 19.0 11.0 26.0 11.0 34.0 76 19.334904127396815 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2340065365333821 1101 2 -0.15735128004582677 1101 3 -3.8613195861057115 1101 4 1.1895943850333808 1101 5 -0.6704635798097023 1101 6 3.621927844264448 1101 7 0.7495251243811865 1101 8 -2.2580417586776527 1101 9 -1.5085011643249153 1101 10-11 -2.217409835137545 1101 12-13 -1.63645482025386 1101 14-15 -1.3107776904586004 1101 16-17 -1.1346098690140174 1101 18-19 -2.5203415649556575 1101 20-21 -0.8443819457488289 1101 22-23 -0.3544719606904394 1101 24-25 -2.8173446488894527 1101 26-27 0.014968037014121194 1101 28-29 -0.4124562059488035 1101 30-31 0.6457607438446225 1101 32-33 -1.1774955034431116 1101 34-35 -2.3090945212755187 1101 36-37 -2.824700149165082 1101 38-39 -2.4334302591846075 1101 40-41 -2.134398999893648 1101 42-43 -2.0929373857433866 1101 44-45 1.9525689790135168 1101 46-47 -1.3035094255061779 1101 48-49 -1.0614236578019458 1101 50-51 -3.265734211857019 1101 52-53 -0.6683268503559532 1101 54-55 -1.6564773716918424 1101 56-57 -4.449277367077027 1101 58-59 -1.1910543367033526 1101 60-61 -2.051953971983785 1101 62-63 0.9813460821202575 1101 64-65 -1.0296928637889948 1101 66-67 1.3871371593008917 1101 68-69 -0.9553394767597325 1101 70-71 -2.5788011517011924 1101 72-73 -1.180277271059765 1101 74-75 -0.4475798400305564 1101 76 -2.721368837694474 1102 1 0.7221017746286194 1102 2 0.3902677675732207 1102 3 -0.037510062296185254 1102 4 -3.955643710204715 1102 5 -1.8490350083811293 1102 6 -0.13045310811650523 1102 7 -1.4290463041902406 1102 8 2.3443391937032985 1102 9 0.41768931186555847 1102 10-11 -1.9186003113280208 1102 12-13 -2.1709786297776716 1102 14-15 -1.9929205476014573 1102 16-17 1.3153901309859855 1102 18-19 0.9915631969491052 1102 20-21 0.32109424472736237 1102 22-23 -0.5699481511666278 1102 24-25 0.395750589205786 1102 26-27 -1.074317677271594 1102 28-29 1.2446866511940513 1102 30-31 -0.32209639901252274 1102 32-33 -2.0739240748716803 1102 34-35 -1.509094521275518 1102 36-37 -0.3473191967841309 1102 38-39 0.08561735986301144 1102 40-41 -1.914160904655553 1102 42-43 -2.439365957171958 1102 44-45 -1.8498119733674372 1102 46-47 0.4048239078271578 1102 48-49 0.3433382469599593 1102 50-51 -1.341924688047495 1102 52-53 -0.25285065987976196 1102 54-55 -3.1981440383585067 1102 56-57 -2.4171345099341686 1102 58-59 -3.186292431941446 1102 60-61 -3.8221920672218808 1102 62-63 -3.3638920131178374 1102 64-65 -1.7725500066461386 1102 66-67 -3.135481888318157 1102 68-69 -1.3458156672359216 1102 70-71 0.396198848298809 1102 72-73 -1.8219439377264344 1102 74-75 -1.8642465066972207 1102 76 -4.040416456742092 1103 1 0.8021883547151987 1103 2 3.2971941744996265 1103 3 -0.33404685883298413 1103 4 -0.4058601604211667 1103 5 1.852263692917571 1103 6 3.0946551169917207 1103 7 1.7722523971084598 1103 8 -1.8807690314049275 1103 9 -2.0130466188703693 1103 10-11 -0.19240983513754628 1103 12-13 0.6158179070188652 1103 14-15 0.6596768549959471 1103 16-17 -0.29824623265037786 1103 18-19 1.7523857077716158 1103 20-21 -1.096654673021554 1103 22-23 -0.8112901425086179 1103 24-25 -0.3196173761621779 1103 26-27 1.1626953097413946 1103 28-29 -0.7260925695851697 1103 30-31 0.2525789256627995 1103 32-33 -1.4570409579885641 1103 34-35 -0.37273088491188133 1103 36-37 -1.45879105825599 1103 38-39 2.648387922633571 1103 40-41 -0.07758081807546802 1103 42-43 1.0638807960747982 1103 44-45 -2.8747037482592113 1103 46-47 1.503308756312002 1103 48-49 0.5749399785616909 1103 50-51 -1.4339160300388407 1103 52-53 -1.2842359412650453 1103 54-55 -0.03147737169184239 1103 56-57 -1.1833682761679327 1103 58-59 -1.386508882157898 1103 60-61 -1.636044881074696 1103 62-63 0.6177097184838942 1103 64-65 1.074852590756457 1103 66-67 1.9803189774827104 1103 68-69 -0.7689758403960951 1103 70-71 0.7143806664806256 1103 72-73 0.3219954562129601 1103 74-75 -0.3339434763941931 1103 76 -1.1486415649671997 1104 1 -2.715993463466617 1104 2 -2.6573512800458268 1104 3 0.2786804138942891 1104 4 -0.020405614966620078 1104 5 0.4595364201902967 1104 6 -3.1380721557355535 1104 7 2.799525124381187 1104 8 3.431958241322345 1104 9 0.26149883567508425 1104 10-11 -1.242409835137547 1104 12-13 1.5985451797461394 1104 14-15 -1.9357776904586004 1104 16-17 -0.9646098690140157 1104 18-19 -1.170341564955656 1104 20-21 -1.7493819457488264 1104 22-23 -0.09947196069043684 1104 24-25 0.1776553511105483 1104 26-27 -1.5400319629858785 1104 28-29 -0.5524562059488005 1104 30-31 1.14076074384462 1104 32-33 0.6775044965568888 1104 34-35 2.5109054787244816 1104 36-37 -0.03970014916508546 1104 38-39 0.7665697408153918 1104 40-41 0.3906010001063507 1104 42-43 -1.6079373857433872 1104 44-45 -0.8574310209864855 1104 46-47 -0.968509425506177 1104 48-49 -1.841423657801947 1104 50-51 -1.1257342118570186 1104 52-53 -1.5033268503559505 1104 54-55 1.4685226283081576 1104 56-57 -0.5542773670770273 1104 58-59 -0.23105433670335174 1104 60-61 -0.34695397198378686 1104 62-63 1.5313460821202582 1104 64-65 0.37030713621100375 1104 66-67 -1.0478628406991106 1104 68-69 -2.6153394767597327 1104 70-71 -0.0838011517011914 1104 72-73 -0.6952772710597657 1104 74-75 -0.9975798400305571 1104 76 1.0186311623055282 1105 1 -0.3621473096204646 1105 2 -1.1188897415842867 1105 3 -1.3305503553364808 1105 4 -1.5142517688127732 1105 5 -1.3820020413481622 1105 6 -3.1396106172740126 1105 7 -1.0389364140803536 1105 8 1.2381120874761926 1105 9 0.9030372972135439 1105 10-11 0.5960517033239938 1105 12-13 2.580852872053832 1105 14-15 -1.0011623058432164 1105 16-17 -0.5692252536294014 1105 18-19 2.537350742736649 1105 20-21 1.1498488234819426 1105 22-23 -1.0910104222288979 1105 24-25 -1.7461908027356046 1105 26-27 -0.725416578370492 1105 28-29 -0.1701485136411094 1105 30-31 -0.1792392561553804 1105 32-33 -2.252495503443111 1105 34-35 1.240905478724482 1105 36-37 -0.3766232260881637 1105 38-39 2.005031279276931 1105 40-41 0.4906010001063521 1105 42-43 2.2666779988720016 1105 44-45 -0.3782002517557146 1105 46-47 -0.005432502429258079 1105 48-49 -1.6383467347250225 1105 50-51 0.5381119419891327 1105 52-53 -0.48178838881749186 1105 54-55 1.660830320615851 1105 56-57 -0.3319696747693328 1105 58-59 0.6012533556043422 1105 60-61 3.273046028016214 1105 62-63 -0.32634622557204906 1105 64-65 0.9087686746725439 1105 66-67 1.4698294669931968 1105 68-69 1.1138912924710382 1105 70-71 0.06927577137573238 1105 72-73 -1.0206618864443797 1105 74-75 3.07165092920021 1105 76 3.078631162305527 1106 1 -2.215993463466617 1106 2 -1.107351280045826 1106 3 1.6886804138942892 1106 4 3.4395943850333808 1106 5 0.4795364201902963 1106 6 -2.128072155735552 1106 7 -1.4004748756188121 1106 8 1.5919582413223452 1106 9 -4.058501164324916 1106 10-11 -1.1674098351375477 1106 12-13 -1.0364548202538586 1106 14-15 -1.9357776904585968 1106 16-17 -1.284609869014016 1106 18-19 -1.045341564955656 1106 20-21 -3.169381945748828 1106 22-23 -1.6794719606904387 1106 24-25 -0.5173446488894484 1106 26-27 -1.310031962985878 1106 28-29 0.9125437940511958 1106 30-31 0.5957607438446182 1106 32-33 0.4975044965568891 1106 34-35 0.26590547872448056 1106 36-37 1.2002998508349165 1106 38-39 -0.15843025918460896 1106 40-41 -1.309398999893645 1106 42-43 0.5070626142566148 1106 44-45 -1.6724310209864832 1106 46-47 -1.4035094255061793 1106 48-49 -2.8364236578019444 1106 50-51 -0.465734211857022 1106 52-53 0.7816731496440497 1106 54-55 -1.181477371691841 1106 56-57 -1.4992773670770276 1106 58-59 0.5089456632966503 1106 60-61 -0.6019539719837859 1106 62-63 -0.5186539178797425 1106 64-65 -1.4046928637889948 1106 66-67 -1.8128628406991112 1106 68-69 -0.08033947675972897 1106 70-71 -1.5288011517011917 1106 72-73 0.7447227289402321 1106 74-75 -0.347579840030555 1106 76 0.8286311623055269 1107 1 -1.2359934634666168 1107 2 -0.6173512800458276 1107 3 -1.2413195861057105 1107 4 -1.02040561496662 1107 5 -1.8604635798097036 1107 6 -3.0980721557355544 1107 7 -1.6804748756188133 1107 8 -4.008041758677653 1107 9 -0.2585011643249153 1107 10-11 -0.46240983513754585 1107 12-13 -0.6414548202538626 1107 14-15 0.8642223095414003 1107 16-17 -1.4246098690140165 1107 18-19 -0.6903415649556592 1107 20-21 0.5706180542511703 1107 22-23 0.5405280393095637 1107 24-25 -0.282344648889449 1107 26-27 -0.520031962985879 1107 28-29 -0.2124562059488042 1107 30-31 -0.8992392561553793 1107 32-33 -0.08249550344310919 1107 34-35 1.490905478724482 1107 36-37 -0.9597001491650836 1107 38-39 -1.273430259184611 1107 40-41 -1.8893989998936505 1107 42-43 3.792062614256615 1107 44-45 2.3025689790135146 1107 46-47 0.1314905744938244 1107 48-49 1.158576342198053 1107 50-51 1.1142657881429798 1107 52-53 0.6766731496440492 1107 54-55 0.6685226283081604 1107 56-57 -1.194277367077028 1107 58-59 -0.431054336703351 1107 60-61 0.113046028016214 1107 62-63 -0.2086539178797402 1107 64-65 0.8703071362110038 1107 66-67 -1.5878628406991098 1107 68-69 0.30466052324026904 1107 70-71 -0.0838011517011914 1107 72-73 -0.33527727105976624 1107 74-75 -1.0575798400305558 1107 76 0.4986311623055286 1108 1 2.90067320320005 1108 2 1.5926487199541732 1108 3 0.6470137472276214 1108 4 0.9312610517000479 1108 5 3.4962030868569656 1108 6 4.113594510931115 1108 7 4.582858457714519 1108 8 3.600291574655678 1108 9 3.983165502341752 1108 10-11 4.849256831529118 1108 12-13 6.242711846412806 1108 14-15 3.197555642874736 1108 16-17 4.5653901309859855 1108 18-19 3.8338251017110103 1108 20-21 3.5056180542511726 1108 22-23 3.933028039309562 1108 24-25 6.261822017777217 1108 26-27 3.056634703680789 1108 28-29 3.679210460717865 1108 30-31 2.737427410511284 1108 32-33 3.997504496556889 1108 34-35 4.990905478724482 1108 36-37 4.991966517501581 1108 38-39 4.758236407482055 1108 40-41 7.511434333439688 1108 42-43 4.444562614256615 1108 44-45 3.6025689790135154 1108 46-47 2.6131572411604864 1108 48-49 2.7094096755313863 1108 50-51 3.6134324548096473 1108 52-53 3.7025064829773804 1108 54-55 2.635189294974822 1108 56-57 3.788222632922974 1108 58-59 3.533945663296649 1108 60-61 3.335546028016214 1108 62-63 2.3563460821202575 1108 64-65 6.141140469544336 1108 66-67 5.7038038259675545 1108 68-69 4.844660523240268 1108 70-71 4.104532181632141 1108 72-73 4.7197227289402335 1108 74-75 3.9690868266361115 1108 76 4.870297828972195 1109 1 1.8476429001697454 1109 2 1.9335578108632632 1109 3 0.484134959348836 1109 4 0.7759580213970168 1109 5 3.443172783826661 1109 6 -0.8144357920991894 1109 7 -1.4095657847097236 1109 8 4.391958241322346 1109 9 -1.2857738915976427 1109 10-11 2.580317437589727 1109 12-13 2.0249088161097752 1109 14-15 -1.1357776904585997 1109 16-17 -0.3437007781049246 1109 18-19 -0.5885233831374777 1109 20-21 -0.3921092184761008 1109 22-23 0.3932553120368354 1109 24-25 2.3394735329287286 1109 26-27 0.5263316733777579 1109 28-29 -1.817001660494256 1109 30-31 -0.08833016524629045 1109 32-33 -1.6388591398067476 1109 34-35 -1.190912703093698 1109 36-37 -0.2542456037105367 1109 38-39 -1.9652484410027888 1109 40-41 -0.7821262726209213 1109 42-43 -0.9133919311979319 1109 44-45 -1.579249202804668 1109 46-47 -0.13305488005163113 1109 48-49 -2.7432418396201257 1109 50-51 0.8388112426884327 1109 52-53 -2.1251450321741316 1109 54-55 -0.7132955535100223 1109 56-57 -0.1606410034406629 1109 58-59 -1.636508882157898 1109 60-61 -1.1133176083474225 1109 62-63 -0.29138119060701584 1109 64-65 -2.6524201365162696 1109 66-67 -0.20149920433547308 1109 68-69 -1.0189758403960951 1109 70-71 0.6007443028442623 1109 72-73 -0.5189136346961298 1109 74-75 1.8024201599694436 1109 76 -3.1940961104217465 1110 1 1.9576907470596971 1110 2 0.4479118778489095 1110 3 0.9123646244206043 1110 4 3.3501207008228526 1110 5 0.8426943149271402 1110 6 -0.5991247873145014 1110 7 1.5258409138548714 1110 8 6.970905609743397 1110 9 2.257288309359293 1110 10-11 3.004958585915084 1110 12-13 0.3490714955356111 1110 14-15 2.127380204278243 1110 16-17 1.7627585520386155 1110 18-19 0.41386896136013007 1110 20-21 0.3148285805669637 1110 22-23 1.673159618256932 1110 24-25 -0.6055025436262937 1110 26-27 -0.4652951208806151 1110 28-29 -1.1914035743698577 1110 30-31 -2.363449782471168 1110 32-33 -0.8972323455483746 1110 34-35 -2.6669892581176207 1110 36-37 -4.694436991270347 1110 38-39 -2.507114469710924 1110 40-41 -0.956767420946278 1110 42-43 1.289957351098721 1110 44-45 0.5236216105924605 1110 46-47 0.150437942914877 1110 48-49 0.04383950009279047 1110 50-51 -1.3920500013307056 1110 52-53 -1.3906952714085818 1110 54-55 0.9948384177818426 1110 56-57 -0.7479615776033413 1110 58-59 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2116 74-75 -4.03091317336389 2116 76 -0.865813282138916 2117 1 2.359006536533382 2117 2 2.9676487199541732 2117 3 1.3136804138942892 2117 4 4.13959438503338 2117 5 2.2045364201902977 2117 6 2.3219278442644473 2117 7 0.8745251243811865 2117 8 1.891958241322346 2117 9 -1.433501164324916 2117 10-11 1.9950901648624537 2117 12-13 -0.6114548202538614 2117 14-15 1.9892223095414003 2117 16-17 2.3153901309859855 2117 18-19 3.2921584350443425 2117 20-21 5.318118054251173 2117 22-23 4.120528039309562 2117 24-25 4.220155351110549 2117 26-27 5.952468037014121 2117 28-29 7.200043794051197 2117 30-31 3.0082607438446196 2117 32-33 0.9350044965568891 2117 34-35 0.865905478724482 2117 36-37 4.0127998508349165 2117 38-39 1.654069740815391 2117 40-41 0.6781010001063521 2117 42-43 2.132062614256615 2117 44-45 5.352568979013515 2117 46-47 5.946490574493822 2117 48-49 2.188576342198054 2117 50-51 1.9467657881429794 2117 52-53 2.5566731496440482 2117 54-55 4.156022628308158 2117 56-57 2.788222632922974 2117 58-59 1.2839456632966488 2117 60-61 0.7730460280162141 2117 62-63 1.1063460821202575 2117 64-65 0.30780713621100375 2117 66-67 2.2246371593008902 2117 68-69 4.782160523240268 2117 70-71 3.021198848298809 2117 72-73 6.1572227289402335 2117 74-75 0.6149201599694436 2117 76 5.703631162305527 2118 1 0.26178431431116067 2118 2 -3.7684623911569375 2118 3 -4.894652919439045 2118 4 -0.3048500594110628 2118 5 4.468425309079187 2118 6 -7.289183266846663 2118 7 -2.6671415422854814 2118 8 1.6141804635445673 2118 9 -2.614056719880473 2118 10-11 -2.3312987240264356 2118 12-13 -2.3058992646983043 2118 14-15 -1.9135554682363782 2118 16-17 -4.6846098690140145 2118 18-19 1.1741028794887853 2118 20-21 -0.5360486124154917 2118 22-23 -3.879471960690438 2118 24-25 -3.8423446488894513 2118 26-27 -1.3044764074303217 2118 28-29 -2.66801176150436 2118 30-31 -1.2347948117109375 2118 32-33 -8.335828836776445 2118 34-35 -2.3979834101644073 2118 36-37 -2.9663668158317478 2118 38-39 -5.3112080369623875 2118 40-41 -2.009398999893648 2118 42-43 -3.201270719076721 2118 44-45 0.6581245345690725 2118 46-47 -2.2062872032839564 2118 48-49 -0.06142365780194581 2118 50-51 -3.6990675451903527 2118 52-53 -2.165549072578173 2118 54-55 -4.587032927247398 2118 56-57 -1.3298329226325833 2118 58-59 -0.535498781147794 2118 60-61 -0.7269539719837859 2118 62-63 -1.2130983623241853 2118 64-65 -3.740803974900107 2118 66-67 -0.6989739518102205 2118 68-69 -1.8220061434263997 2118 70-71 -3.603801151701191 2118 72-73 -2.4886106043930987 2118 74-75 -2.0864687289194457 2118 76 -2.6435910599166945 2119 1 5.484006536533382 2119 2 10.542648719954173 2119 3 9.43868041389429 2119 4 3.7395943850333815 2119 5 11.379536420190298 2119 6 4.821927844264447 2119 7 3.599525124381188 2119 8 5.391958241322346 2119 9 2.5414988356750854 2119 10-11 4.257590164862457 2119 12-13 5.23854517974614 2119 14-15 4.5642223095414 2119 16-17 4.715390130985984 2119 18-19 2.629658435044341 2119 20-21 5.230618054251174 2119 22-23 4.420528039309563 2119 24-25 6.057655351110547 2119 26-27 4.939968037014122 2119 28-29 5.387543794051197 2119 30-31 3.920760743844621 2119 32-33 4.997504496556889 2119 34-35 4.290905478724483 2119 36-37 4.400299850834916 2119 38-39 3.8665697408153896 2119 40-41 3.290601000106353 2119 42-43 4.532062614256613 2119 44-45 2.502568979013514 2119 46-47 2.6714905744938235 2119 48-49 4.038576342198056 2119 50-51 4.034265788142978 2119 52-53 6.15667314964405 2119 54-55 4.1685226283081604 2119 56-57 2.425722632922973 2119 58-59 6.908945663296649 2119 60-61 3.6730460280162127 2119 62-63 1.9313460821202568 2119 64-65 8.770307136211002 2119 66-67 7.012137159300892 2119 68-69 6.44466052324027 2119 70-71 6.396198848298809 2119 72-73 4.144722728940234 2119 74-75 4.002420159969443 2119 76 -0.6213688376944724 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 12.0 13 90.0 14 260.0 15 453.0 16 592.0 17 674.0 18 768.0 19 758.0 20 642.0 21 476.0 22 336.0 23 205.0 24 146.0 25 97.0 26 67.0 27 40.0 28 46.0 29 34.0 30 27.0 31 19.0 32 36.0 33 41.0 34 46.0 35 65.0 36 106.0 37 118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.98775510204082 28.783673469387754 8.538775510204081 35.689795918367345 2 5.004874878128047 24.098147546311342 25.836854078648035 45.06012349691258 3 5.88235294117647 27.250568735781606 35.26161845953851 31.60545986350341 4 3.3799155021124476 45.6776080597985 20.13324666883328 30.80922976925577 5 4.41988950276243 32.59668508287293 35.24536886577835 27.738056548586286 6 6.12609684757881 30.906727331816704 12.333441663958402 50.633734156646085 7 5.151121221969451 48.71628209294768 10.399740006499837 35.73285667858303 8 4.289892752681182 37.03282417939551 12.950926226844327 45.726356841078974 9 9.0835229119272 31.004224894377643 11.992200194995126 47.92005199870003 10-11 6.832954176145596 32.16607084822879 22.84692882677933 38.15404614884628 12-13 6.719207019824504 28.623659408514783 22.749431264218394 41.907702307442314 14-15 12.195320116997076 31.897952551186222 17.647058823529413 38.25966850828729 16-17 15.583360415989599 31.296717582060445 17.93955151121222 35.18037049073774 18-19 12.390315242118948 28.306792330191744 17.59018524536887 41.71270718232044 20-21 10.131621709457264 29.42801429964251 18.678908027299318 41.76145596360091 22-23 17.671433214169646 29.25739356516087 15.347741306467338 37.723431914202145 24-25 13.503412414689633 34.4166395840104 14.632759181020475 37.44718882027949 26-27 10.805979850503737 35.72473188170296 19.987000324991875 33.48228794280143 28-29 13.982775430614236 30.370490737731558 23.46441338966526 32.18232044198895 30-31 13.617159571010726 29.65550861228469 24.601884952876176 32.12544686382841 32-33 10.334741631459215 35.52973675658109 22.213194670133245 31.922326941826455 34-35 9.725381865453365 37.15469613259669 19.775755606109847 33.344166395840105 36-37 11.780955476113098 31.38609034774131 21.6769580760481 35.1559961000975 38-39 13.007799805004874 24.601884952876176 27.35619109522262 35.03412414689633 40-41 9.798505037374065 27.827429314267143 25.211244718882025 37.162820929476766 42-43 5.866103347416315 25.39811504712382 27.42118947026324 41.31459213519662 44-45 6.954826129346767 25.4062398440039 31.889827754306143 35.74910627234319 46-47 7.621059473513162 31.231719207019825 27.088072798180047 34.05914852128697 48-49 7.442313942151446 34.2297692557686 21.530711732206694 36.79720506987325 50-51 7.458563535911603 32.80792980175495 19.15014624634384 40.5833604159896 52-53 6.142346441338967 33.96165095872603 19.670133246668833 40.22586935326617 54-55 6.646083847903802 30.939226519337016 19.80825479363016 42.60643483912902 56-57 10.992850178745531 31.946701332466688 18.573285667858304 38.48716282092948 58-59 9.327266818329543 31.426714332141696 17.33019174520637 41.91582710432239 60-61 7.417939551511213 28.241793955151124 19.507637309067274 44.832629184270395 62-63 10.350991225219369 29.14364640883978 18.102047448813778 42.40331491712707 64-65 9.57913552161196 30.386740331491712 14.510887227819305 45.52323691907702 66-67 8.059798505037374 36.65908352291193 12.325316867078323 42.95580110497237 68-69 10.090997725056873 37.01657458563536 12.398440038999025 40.493987650308746 70-71 11.68345791355216 35.440363990900224 12.309067273318167 40.567110822229445 72-73 10.619109522261944 37.024699382515436 11.797205069873252 40.55898602534936 74-75 10.927851803704907 33.31979200519987 14.429639259018526 41.322716932076695 76 8.953526161845954 28.144296392590185 12.625934351641208 50.27624309392266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 2.0 15 3.0 16 3.0 17 3.0 18 4.0 19 5.0 20 6.5 21 8.0 22 9.0 23 11.0 24 13.0 25 14.0 26 13.5 27 13.5 28 17.5 29 21.0 30 42.0 31 55.0 32 56.0 33 65.0 34 68.5 35 94.5 36 130.5 37 144.0 38 153.5 39 177.5 40 207.5 41 256.0 42 289.0 43 293.0 44 332.0 45 369.0 46 371.0 47 378.5 48 389.5 49 388.0 50 383.0 51 370.0 52 334.5 53 317.5 54 323.0 55 286.5 56 249.0 57 230.5 58 213.0 59 201.0 60 160.5 61 120.0 62 108.0 63 93.5 64 69.5 65 56.5 66 39.0 67 25.0 68 25.0 69 24.0 70 22.0 71 21.0 72 16.5 73 10.5 74 7.0 75 5.0 76 3.5 77 2.5 78 2.0 79 1.0 80 2.0 81 3.5 82 3.0 83 2.0 84 2.0 85 2.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4712382190445239 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6154.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.74130646733832 #Duplication Level Percentage of deduplicated Percentage of total 1 98.48711554447215 96.26259343516412 2 0.9808811305070657 1.9174520636984078 3 0.399002493765586 1.1699707507312318 4 0.0831255195344971 0.3249918752031199 5 0.0 0.0 6 0.01662510390689942 0.09749756256093597 7 0.03325020781379884 0.22749431264218392 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGCAGTCCGGTGTAGATCTCGGTG 7 0.11374715632109196 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG 7 0.11374715632109196 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016249593760155997 0.0 2 0.0 0.0 0.0 0.016249593760155997 0.0 3 0.0 0.0 0.0 0.016249593760155997 0.0 4 0.0 0.0 0.0 0.016249593760155997 0.0 5 0.0 0.0 0.0 0.016249593760155997 0.0 6 0.0 0.0 0.0 0.016249593760155997 0.0 7 0.0 0.0 0.0 0.016249593760155997 0.0 8 0.0 0.0 0.0 0.016249593760155997 0.0 9 0.0 0.0 0.0 0.016249593760155997 0.0 10 0.0 0.0 0.0 0.016249593760155997 0.0 11 0.0 0.0 0.0 0.016249593760155997 0.0 12 0.0 0.0 0.0 0.032499187520311994 0.0 13 0.0 0.0 0.0 0.04874878128046799 0.0 14 0.0 0.0 0.0 0.06499837504062399 0.0 15 0.0 0.0 0.0 0.06499837504062399 0.0 16 0.0 0.0 0.0 0.06499837504062399 0.0 17 0.0 0.0 0.0 0.06499837504062399 0.0 18 0.0 0.0 0.0 0.11374715632109197 0.0 19 0.0 0.0 0.0 0.3412414689632759 0.0 20 0.0 0.0 0.0 0.5199870003249919 0.0 21 0.0 0.0 0.0 0.5199870003249919 0.0 22 0.0 0.0 0.0 0.5849853753656159 0.0 23 0.0 0.0 0.0 0.5849853753656159 0.0 24 0.0 0.0 0.0 0.6662333441663958 0.0 25 0.0 0.0 0.0 0.7149821254468638 0.0 26 0.0 0.0 0.0 0.7149821254468638 0.0 27 0.0 0.0 0.0 0.7312317192070198 0.0 28 0.0 0.0 0.0 0.7474813129671758 0.0 29 0.0 0.0 0.0 0.7637309067273318 0.0 30 0.0 0.0 0.0 0.8124796880077998 0.0 31 0.0 0.0 0.0 0.8287292817679558 0.0 32 0.0 0.0 0.0 0.8612284692882678 0.0 33 0.0 0.0 0.0 0.8774780630484238 0.0 34 0.0 0.0 0.0 0.9587260318492038 0.0 35 0.0 0.0 0.0 1.0724731881702958 0.0 36 0.0 0.0 0.0 1.1374715632109198 0.0 37 0.0 0.0 0.0 1.1862203444913877 0.0 38 0.0 0.0 0.0 1.5112122196945077 0.0 39 0.0 0.0 0.0 1.8199545011374716 0.0 40 0.0 0.0 0.0 1.9987000324991875 0.0 41 0.0 0.0 0.0 2.2261943451413715 0.0 42 0.0 0.0 0.0 2.5349366265843356 0.0 43 0.0 0.0 0.0 2.6974325641858954 0.0 44 0.0 0.0 0.0 2.8761780955476115 0.0 45 0.0 0.0 0.0 3.006174845628859 0.0 46 0.0 0.0 0.0 3.0874228144296394 0.0 47 0.0 0.0 0.0 3.201169970750731 0.0 48 0.0 0.0 0.0 3.2824179395515114 0.0 49 0.0 0.0 0.0 3.3474163145921354 0.0 50 0.0 0.0 0.0 3.509912252193695 0.0 51 0.0 0.0 0.0 3.656158596035099 0.0 52 0.0 0.0 0.0 3.7536561585960353 0.0 53 0.0 0.0 0.0 4.046148846278843 0.0 54 0.0 0.0 0.0 4.127396815079623 0.0 55 0.0 0.0 0.0 4.257393565160871 0.0 56 0.0 0.0 0.0 4.501137471563211 0.0 57 0.0 0.0 0.0 4.549886252843679 0.0 58 0.0 0.0 0.0 4.598635034124147 0.0 59 0.0 0.0 0.0 4.614884627884303 0.0 60 0.0 0.0 0.0 4.744881377965551 0.0 61 0.0 0.0 0.0 4.826129346766331 0.0 62 0.0 0.0 0.0 4.939876503087423 0.0 63 0.0 0.0 0.0 4.972375690607735 0.0 64 0.0 0.0 0.0 5.0373740656483585 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACA 65 1.7860675E-5 32.307693 2 CTTATAC 65 1.7860675E-5 32.307693 1 TATACAC 100 8.85484E-9 31.500002 3 ATACACA 110 2.242632E-8 28.636362 4 ACACATC 145 1.1306838E-8 24.13793 6 TACACAT 135 1.6377817E-7 23.333332 5 CCGACGA 80 0.0023801632 21.875 21 CACATCT 115 3.3302218E-5 21.304348 7 >>END_MODULE