FastQCFastQC Report
Wed 25 May 2016
SRR1780720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6154
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT217335.31036724081898No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT62910.220994475138122RNA PCR Primer, Index 24 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG3014.8911277218069555No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1462.3724406889827754No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1111.8037049073773157RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT1081.7549561260968474No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1011.6412089697757555No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT751.2187195320116997RNA PCR Primer, Index 24 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT731.1862203444913877No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC480.7799805004874878No Hit
CTTATACACATCTCCGAGCCCACGCGACGTGATAGCATCTCGTATGCCGT360.5849853753656159No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC330.5362365940851478No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC290.4712382190445239No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT230.3737406564835879No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG230.3737406564835879No Hit
ACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCT210.3412414689632759RNA PCR Primer, Index 24 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTAGTATGCCGT210.3412414689632759No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGTCTT200.3249918752031199No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTCTT200.3249918752031199No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG190.30874228144296395No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGT190.30874228144296395No Hit
CTTATACACATCTCCGAGCACACGAGACGTGATAGCATCTCGTATGCCGT190.30874228144296395No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCC170.2762430939226519No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTCTT150.24374390640233995No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAAATCGTATGCCGT150.24374390640233995No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC140.22749431264218392No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATTTCGTATGCCGT140.22749431264218392No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT130.21124471888202798No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT130.21124471888202798No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT130.21124471888202798No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC130.21124471888202798RNA PCR Primer, Index 24 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC130.21124471888202798No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCTAGCATCTCGTATGCCGT130.21124471888202798No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG130.21124471888202798No Hit
CTTATACACATCTCCGAGCCCACGTGACGTGATAGCATCTCGTATGCCGT130.21124471888202798No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCACGTATGCCGT120.19499512512187195No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCC120.19499512512187195No Hit
CTTATACACATCTCCGAGCCCAAGAGACGTGATAGCATCTCGTATGCCGT120.19499512512187195No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTGATAGCATCTCGTATGCCGT120.19499512512187195No Hit
CTTATACACATCTCCGCGCCCACGAGACGTGATAGCATCTCGTATGCCGT110.17874553136171598No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG110.17874553136171598No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG110.17874553136171598No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGATAGCATCTCGTATGCCGT100.16249593760155995No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGT100.16249593760155995No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCCCGTATGCCGT100.16249593760155995No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG90.14624634384140398No Hit
ATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTT90.14624634384140398RNA PCR Primer, Index 34 (96% over 28bp)
CTTATACACATCTCCGAGCCAACGAGACGTGATAGCATCTCGTATGCCGT90.14624634384140398No Hit
CTTATACACATCTACGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT90.14624634384140398No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAGCATCTCGTATGCCGT90.14624634384140398No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT90.14624634384140398No Hit
CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT80.12999675008124797No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT80.12999675008124797No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT80.12999675008124797No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG80.12999675008124797No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTC70.11374715632109196No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGA70.11374715632109196No Hit
ATACACATCTCCGAGCCCACGAGAAGTGATAGCATCTCGTATGCCGTCTT70.11374715632109196RNA PCR Primer, Index 34 (95% over 22bp)
CTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTTGA70.11374715632109196RNA PCR Primer, Index 24 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACATCGTATGCCGT70.11374715632109196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAA150.00212087370.0000160
ACACAAA150.00212087370.0000170
ATCGTAT207.319314E-570.039
TTATACA4200.054.1666642
CTTATAC4250.053.529411
GTATGAC200.006629393452.542
ACGTCTT200.006629393452.547
ATGACGT200.006629393452.544
TATGACG200.006629393452.543
GACGTCT200.006629393452.546
CGTATGA200.006629393452.541
TGACGTC200.006629393452.545
TATACAC4300.052.0930253
GAAAAAC305.432729E-446.66666860
AACACAA305.432729E-446.66666869
AAAACAC551.0510303E-744.54545267
TTCTGCT5050.042.2772352
GTCTTCT5250.042.049
TCTTCTG5250.042.050
CGTCTTC5250.042.048
Produced by FastQC (version 0.11.5)