FastQCFastQC Report
Wed 25 May 2016
SRR1780719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96426
Sequences flagged as poor quality0
Sequence length50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1330.13792960404870055No Hit
TCTCTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCG1230.12755895712774562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA800.035.698141
CCGACAA250.002317055135.27708421
CCTTCTG250.002364853635.1307079
GATCTCT451.040029E-634.15485441
CTTATAA551.3688987E-731.9535771
CTGTGTA353.1649385E-431.48099934
CTTCTAC454.0713814E-529.2907811
TCTTTAT300.00575986629.2907811
TTTACAC1100.027.9448782
CTATACA655.91097E-727.0376431
TATACCC801.3086355E-724.7012773
ATCTCTG450.00140494924.39632242
CGGTGGT1350.024.39632243
TTACACA1550.024.0815323
TGACGCG551.5665583E-424.02752713
CGAGAAC752.038927E-623.45696825
GAGAACG803.5875892E-621.97949226
AAGTATG1004.5582055E-821.9794927
TTATACC803.6201E-621.9566922
ATACCCA803.6201E-621.9566924
Produced by FastQC (version 0.11.5)