FastQCFastQC Report
Wed 25 May 2016
SRR1780719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96426
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5550657.563312799452426No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT89299.259950635720656No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG84028.713417542986331No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC54785.68104038329911No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC14111.4632982805467405No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT10921.1324746437682782No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC9290.9634330989567129No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC6350.658536079480638No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3520.3650467716176135No Hit
ACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCT3450.35778731877294506Illumina PCR Primer Index 8 (95% over 22bp)
ATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTT3130.3246012486258893RNA PCR Primer, Index 39 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC2940.3048970194760749No Hit
CATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCT2630.27274801402111465Illumina PCR Primer Index 8 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATATCGTATGCCGT2550.2644514964843507No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC2460.25511791425549124No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGC1780.18459751519299775No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1600.16593035073527887No Hit
TCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTTG1570.16281915665899238Illumina PCR Primer Index 8 (96% over 27bp)
CTTATAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1550.16074502727480142No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1190.12341069835936366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA254.3750828E-544.0633771
TATAACA254.415538E-543.9948123
TTATAAC254.415538E-543.9948122
TAACACA254.415538E-543.9948125
TGCAGAA207.825142E-443.9948122
AACACAT302.5056033E-643.9948126
CTTTACA1850.042.8724751
TTTACAC1900.041.67932
TCTTTAT700.040.9159931
CTATACA1000.039.657041
CCGTCTT9150.039.18663844
GCGTCAG357.1499544E-637.768611
TTACACA2100.037.709843
ATGCCGT70750.035.2269444
TATCGTA702.7284841E-1134.56735238
AATATCG702.7284841E-1134.56735236
ATATCGT702.7284841E-1134.56735237
CTTATAC74600.033.697261
GCCGTCT10650.033.66739343
CTTTATA850.033.6430932
Produced by FastQC (version 0.11.5)