##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780718_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7253 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.00206810974769 33.0 21.0 34.0 12.0 34.0 2 28.1056114711154 34.0 23.0 34.0 11.0 34.0 3 28.505170274369227 34.0 23.0 34.0 11.0 34.0 4 28.65379842823659 34.0 23.0 34.0 11.0 34.0 5 28.749896594512617 34.0 23.0 34.0 11.0 34.0 6 30.754584309940714 37.0 26.0 38.0 11.0 38.0 7 30.581000965117884 37.0 26.0 38.0 11.0 38.0 8 30.303047015028262 36.0 25.0 38.0 11.0 38.0 9 30.794016269130015 37.0 26.0 38.0 12.0 38.0 10-11 30.67820212325934 37.0 26.0 38.0 11.0 38.0 12-13 30.77685095822418 37.0 26.0 38.0 11.0 38.0 14-15 30.534813180752792 37.0 26.0 38.0 11.0 38.0 16-17 30.22887081207776 37.0 23.0 38.0 11.0 38.0 18-19 30.400592858127673 37.0 24.5 38.0 11.0 38.0 20-21 30.441610368123534 37.0 24.5 38.0 11.0 38.0 22-23 30.182338342754722 36.5 23.0 38.0 11.0 38.0 24-25 30.39376809596029 37.0 23.5 38.0 11.0 38.0 26-27 30.402385219909004 37.0 23.5 38.0 11.0 38.0 28-29 30.485661105749344 37.0 24.0 38.0 11.0 38.0 30-31 30.375568730180618 37.0 23.5 38.0 11.0 38.0 32-33 30.082862263890803 37.0 23.0 38.0 11.0 38.0 34-35 30.358541293257964 37.0 24.0 38.0 11.0 38.0 36-37 30.2199090031711 37.0 23.5 38.0 11.0 38.0 38-39 30.368606094030056 37.0 23.5 38.0 11.0 38.0 40-41 30.23280022059837 37.0 24.0 38.0 11.0 38.0 42-43 30.466565559079 37.0 24.5 38.0 11.0 38.0 44-45 29.72632014338894 36.0 22.0 38.0 11.0 38.0 46-47 30.322969805597683 37.0 24.5 38.0 11.0 38.0 48-49 30.331724803529575 37.0 24.5 38.0 11.0 38.0 50-51 30.145388115262648 36.5 23.5 38.0 11.0 38.0 52-53 29.991658624017646 36.0 23.5 38.0 11.0 38.0 54-55 29.978836343581968 36.0 23.0 38.0 11.0 38.0 56-57 29.828898386874396 36.5 23.0 38.0 11.0 38.0 58-59 29.84116917137736 36.0 23.0 38.0 11.0 38.0 60-61 29.836205707982906 36.0 23.0 38.0 11.0 38.0 62-63 29.707155659727007 36.0 23.0 38.0 11.0 38.0 64-65 29.9605680408107 36.0 23.5 38.0 11.0 38.0 66-67 29.885495656969532 36.0 23.0 38.0 11.0 38.0 68-69 29.39852474837998 35.5 22.0 38.0 11.0 38.0 70-71 29.358196608300013 36.0 22.0 38.0 11.0 38.0 72-73 29.554046601406313 36.0 23.0 38.0 11.0 38.0 74-75 28.885978215910658 34.5 21.5 38.0 11.0 38.0 76 28.23548876327037 34.0 20.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.2824739413213457 1101 2 -0.38537476956233974 1101 3 -1.7955729719066156 1101 4 0.2205421416453177 1101 5 -1.7093381033810076 1101 6 -3.006982085992732 1101 7 1.2244525722186133 1101 8 1.2735451722293831 1101 9 0.21311330352071067 1101 10-11 -1.1096019305058924 1101 12-13 0.3146960616151162 1101 14-15 0.7151440783740313 1101 16-17 -0.4867294510023754 1101 18-19 -0.05688236495934618 1101 20-21 1.6353772298057123 1101 22-23 0.32159211833822 1101 24-25 -2.129093121649756 1101 26-27 2.247673512824072 1101 28-29 2.4459666159247213 1101 30-31 2.8586834843443043 1101 32-33 0.4412323480923881 1101 34-35 1.0584177483996449 1101 36-37 1.4304303993559202 1101 38-39 0.23539283026994795 1101 40-41 1.1422822036763947 1101 42-43 0.9131731895040289 1101 44-45 1.7782247634248556 1101 46-47 0.9139679465794934 1101 48-49 -0.7035322925482674 1101 50-51 0.7327494278240074 1101 52-53 1.8033716024349218 1101 54-55 0.5909421204673819 1101 56-57 0.2923860633116995 1101 58-59 -0.4493969521577945 1101 60-61 2.5443385965126097 1101 62-63 1.0740665448273 1101 64-65 1.23692078720158 1101 66-67 -0.9873508271150975 1101 68-69 2.9487189929573 1101 70-71 2.932041285301249 1101 72-73 3.10992612426287 1101 74-75 1.9400433194661737 1101 76 1.6574227643146884 1102 1 3.1088304065047403 1102 2 2.2667991434811405 1102 3 2.4652965933107787 1102 4 3.4814117068627084 1102 5 3.116748853140731 1102 6 3.1669309574855298 1102 7 1.267930833088176 1102 8 0.23006691135981683 1102 9 1.3870263469989723 1102 10-11 0.28170241732019363 1102 12-13 -2.6418256775153175 1102 14-15 -0.241377660756406 1102 16-17 -0.3128164075241173 1102 18-19 0.5952915480841305 1102 20-21 0.46146418632745423 1102 22-23 1.1911573357295246 1102 24-25 2.544819921828509 1102 26-27 -3.4697177915237525 1102 28-29 0.8590100941855923 1102 30-31 3.163031310431265 1102 32-33 -5.406593738864135 1102 34-35 0.9497220962257309 1102 36-37 2.0173869210950492 1102 38-39 2.4745232650525537 1102 40-41 2.0553256819372656 1102 42-43 2.1305644938518533 1102 44-45 -4.417427410488187 1102 46-47 1.5226635987534074 1102 48-49 2.100815533538686 1102 50-51 -4.506381006958602 1102 52-53 2.2381542111305777 1102 54-55 1.547463859597812 1102 56-57 -0.07717915407960518 1102 58-59 1.5071247869726427 1102 60-61 1.1530342486865237 1102 62-63 -2.838976933433571 1102 64-65 0.3673555698102753 1102 66-67 0.6648230859283792 1102 68-69 -3.138237528781829 1102 70-71 0.4103021548664678 1102 72-73 2.6316652546976513 1102 74-75 -3.3643045066207833 1102 76 -1.994751148728792 1103 1 0.7674455433807452 1103 2 -0.9522024668248186 1103 3 -0.03067764179389343 1103 4 0.13197531394805395 1103 5 2.5048325890505545 1103 6 1.8577522135241757 1103 7 1.395145003780609 1103 8 0.1898092624065164 1103 9 2.4772034806543672 1103 10-11 1.4837958150657649 1103 12-13 0.12065419366020436 1103 14-15 0.53076404616791 1103 16-17 -0.9480821080072062 1103 18-19 2.1065636897910593 1103 20-21 0.8125108852002398 1103 22-23 2.4721557254235655 1103 24-25 0.849972900894528 1103 26-27 -0.29338928910829054 1103 28-29 1.5361437495801162 1103 30-31 0.7483775262122592 1103 32-33 -4.2463682960299955 1103 34-35 -0.24270946577104624 1103 36-37 -1.1790704057970594 1103 38-39 0.9463429107852477 1103 40-41 0.4144239106007106 1103 42-43 1.7110797917584577 1103 44-45 -1.41018103367659 1103 46-47 0.7617940335360167 1103 48-49 1.0750506382085767 1103 50-51 1.9493355791927662 1103 52-53 -0.9277073025570246 1103 54-55 1.0981884972789757 1103 56-57 1.9832073193503454 1103 58-59 0.5626803425281999 1103 60-61 -0.458076862424587 1103 62-63 -0.43962105581682565 1103 64-65 1.974440916026058 1103 66-67 1.9329390279573673 1103 68-69 -2.478817238926755 1103 70-71 2.0640863738680757 1103 72-73 0.48351710654950253 1103 74-75 -0.05271030372222896 1103 76 0.031013746601320946 1104 1 1.484482580417783 1104 2 3.1485382739159213 1104 3 3.037470506354257 1104 4 3.1927160546887947 1104 5 2.226314070532034 1104 6 3.759974435746397 1104 7 4.716626485262093 1104 8 4.388327780925035 1104 9 3.435721999172884 1104 10-11 2.4008328521027984 1104 12-13 3.3199134529194616 1104 14-15 2.8055788609827275 1104 16-17 2.3941401142150163 1104 18-19 3.321378504605871 1104 20-21 2.0510294037187577 1104 22-23 3.972896466164311 1104 24-25 3.1796025305241606 1104 26-27 2.815499599780594 1104 28-29 3.0746622680986313 1104 30-31 2.9291182669529974 1104 32-33 3.029928000266306 1104 34-35 4.135809052747472 1104 36-37 2.7912999645733123 1104 38-39 2.5893058737482093 1104 40-41 3.369238725415528 1104 42-43 3.1940427547214156 1104 44-45 0.21648563299007506 1104 46-47 2.4417940335360164 1104 48-49 2.8869024900604288 1104 50-51 4.459705949563137 1104 52-53 4.308588993739271 1104 54-55 1.9048551639456406 1104 56-57 2.9054295415725697 1104 58-59 4.407124786972641 1104 60-61 2.3530342486865266 1104 62-63 3.0366752404794717 1104 64-65 1.9351816567667974 1104 66-67 3.239605694624032 1104 68-69 -0.3121505722600908 1104 70-71 3.123345633127336 1104 72-73 2.7916652546976515 1104 74-75 3.3539563629444373 1104 76 1.9391618947494713 1105 1 -4.231231705296501 1105 2 -4.211461726084078 1105 3 -2.3825294936457446 1105 4 -3.270141088168348 1105 5 -2.22797164375368 1105 6 -1.3128827071107452 1105 7 -2.1590878004521947 1105 8 -4.684529361932107 1105 9 -1.1828494293985408 1105 10-11 -3.9970242907543394 1105 12-13 -3.0486579756519667 1105 14-15 -5.422278281874419 1105 16-17 -4.083002742927842 1105 18-19 -2.8286214953941275 1105 20-21 -4.168970596281241 1105 22-23 -3.7513892481214093 1105 24-25 -3.181111755190127 1105 26-27 -2.3330718287908354 1105 28-29 -2.641766303329934 1105 30-31 -4.135881733047 1105 32-33 -3.4322148568765556 1105 34-35 -2.581333804395385 1105 36-37 -4.558700035426689 1105 38-39 0.05216301660535194 1105 40-41 -0.860046988870188 1105 42-43 -0.9152429595642957 1105 44-45 -0.4399429384384952 1105 46-47 -0.18463453789255269 1105 48-49 -0.3230975099395721 1105 50-51 -1.9660083361511482 1105 52-53 -2.6671252919750117 1105 54-55 -4.181573407482929 1105 56-57 -1.6517133155702908 1105 58-59 -1.5464466415987879 1105 60-61 -1.1505371798849033 1105 62-63 -4.6761666338213104E-4 1105 64-65 -1.9276754860903473 1105 66-67 -3.0432514482331143 1105 68-69 -3.937150572260091 1105 70-71 -1.5337972240155224 1105 72-73 -0.45762045958806397 1105 74-75 -4.368186494198419 1105 76 -3.106552390964815 1106 1 3.7092444851796884 1106 2 2.883776369154017 1106 3 1.2603276492113977 1106 4 2.1108112927840317 1106 5 3.605361689579656 1106 6 4.222831578603543 1106 7 3.1147217233573308 1106 8 4.851184923782181 1106 9 3.329055332506222 1106 10-11 6.258928090198044 1106 12-13 3.8084848814908945 1106 14-15 3.869388384792245 1106 16-17 5.655092495167395 1106 18-19 3.6118546950820622 1106 20-21 2.7119817846711385 1106 22-23 1.6652774185452586 1106 24-25 4.955793006714632 1106 26-27 4.238356742637734 1106 28-29 2.8165670300033945 1106 30-31 2.5843563621910945 1106 32-33 4.1689756193139225 1106 34-35 3.9662852432236626 1106 36-37 4.14368091695426 1106 38-39 5.52835349279583 1106 40-41 4.300667296844097 1106 42-43 5.197852278530945 1106 44-45 5.756485632990074 1106 46-47 5.285603557345535 1106 48-49 3.9983310614889973 1106 50-51 4.8673249971821875 1106 52-53 3.773350898501178 1106 54-55 3.9791408782313553 1106 56-57 4.062572398715428 1106 58-59 3.911886691734548 1106 60-61 5.3911294867817645 1106 62-63 5.332865716669954 1106 64-65 5.429467371052514 1106 66-67 5.290081885100221 1106 68-69 4.80689704678753 1106 70-71 4.751917061698766 1106 72-73 4.964998588030987 1106 74-75 1.3044325534206251 1106 76 3.0363047518923274 1107 1 1.0218738847656077 1107 2 -2.602766073910164 1107 3 -2.2738338414718307 1107 4 -1.3011969887894672 1107 5 -0.6223815816418785 1107 6 -2.267851651210126 1107 7 -0.4711996016944333 1107 8 2.273545172229383 1107 9 1.778330694825062 1107 10-11 0.3469198086245413 1107 12-13 1.1190438877020732 1107 14-15 2.2368832088088126 1107 16-17 0.839357505519363 1107 18-19 -0.8612301910462996 1107 20-21 -0.9950575528029795 1107 22-23 0.16941820529473972 1107 24-25 0.19699383487198574 1107 26-27 1.0520213389110324 1107 28-29 -1.2062072971187554 1107 30-31 -3.380446950438305 1107 32-33 -1.537028521472827 1107 34-35 -1.5067996429046993 1107 36-37 -0.3304391658614705 1107 38-39 0.9093058737482096 1107 40-41 0.7944561167198749 1107 42-43 0.7392601460257673 1107 44-45 1.299963893859637 1107 46-47 -1.499075531681374 1107 48-49 0.3399459683212953 1107 50-51 1.3197059495631365 1107 52-53 0.04250203721753465 1107 54-55 -0.49601440127175067 1107 56-57 -0.3597878497317808 1107 58-59 1.5940813087117753 1107 60-61 0.19651250955609 1107 62-63 0.5740665448272999 1107 64-65 -0.3500357345375491 1107 66-67 0.5996056946240316 1107 68-69 0.2530668190442569 1107 70-71 0.14943258964907358 1107 72-73 -1.3248564844327824 1107 74-75 -3.1034349414033926 1107 76 -3.3425772356853116 1108 1 -0.1955174195822167 1108 2 1.3885382739159233 1108 3 0.6774705063542541 1108 4 -1.4072839453112067 1108 5 -1.493685929467965 1108 6 -0.8000255642536018 1108 7 -0.36337351473790847 1108 8 -0.531672219074963 1108 9 1.3557219991728857 1108 10-11 0.5608328521028021 1108 12-13 1.5999134529194627 1108 14-15 -0.31442113901727353 1108 16-17 0.7141401142150166 1108 18-19 2.1813785046058705 1108 20-21 -1.8289705962812413 1108 22-23 2.172896466164307 1108 24-25 2.1596025305241575 1108 26-27 1.6154995997805948 1108 28-29 1.6346622680986336 1108 30-31 0.5291182669529988 1108 32-33 1.4699280002663038 1108 34-35 0.3558090527474711 1108 36-37 1.5112999645733112 1108 38-39 1.009305873748211 1108 40-41 0.42923872541552655 1108 42-43 0.9740427547214168 1108 44-45 0.01648563299007222 1108 46-47 0.9817940335360156 1108 48-49 0.16690249006042635 1108 50-51 2.1397059495631368 1108 52-53 -0.11141100626072742 1108 54-55 0.76485516394564 1108 56-57 0.40542954157256617 1108 58-59 0.16712478697264288 1108 60-61 -0.6669657513134766 1108 62-63 -0.4633247595205283 1108 64-65 1.075181656766798 1108 66-67 1.0396056946240329 1108 68-69 1.887849427739912 1108 70-71 1.1433456331273355 1108 72-73 1.3316652546976542 1108 74-75 1.0939563629444322 1108 76 -0.14083810525053053 1109 1 -0.238995680451783 1109 2 -2.0375486826058165 1109 3 0.37834007157164606 1109 4 -1.5185882931372916 1109 5 0.6819662444450785 1109 6 -0.31132991207968885 1109 7 -1.12337351473791 1109 8 -1.3786287408140936 1109 9 -0.13471278343580906 1109 10-11 2.020832852102803 1109 12-13 2.4233917137890266 1109 14-15 0.12818755663489867 1109 16-17 0.4915314185628432 1109 18-19 0.07355241764934917 1109 20-21 1.4179859254578915 1109 22-23 1.0172442922512595 1109 24-25 1.8056894870458962 1109 26-27 2.052021338911029 1109 28-29 1.4894448767942876 1109 30-31 1.945640006083437 1109 32-33 1.484710608961958 1109 34-35 2.6888525310083473 1109 36-37 1.8652130080515725 1109 38-39 2.0832189172264677 1109 40-41 0.6422822036763947 1109 42-43 2.2175210155909824 1109 44-45 1.4956160677726835 1109 46-47 2.8052722944055795 1109 48-49 1.492119881364772 1109 50-51 2.8197059495631365 1109 52-53 1.3903281241740544 1109 54-55 1.3083334248152028 1109 56-57 1.6184730198334343 1109 58-59 1.3984291347987288 1109 60-61 2.0443385965126097 1109 62-63 -2.2302812812596606 1109 64-65 -0.6109052997549433 1109 66-67 0.9256926511457735 1109 68-69 1.1878494277399092 1109 70-71 0.8668238939969015 1109 72-73 -0.10746518008495798 1109 74-75 1.8965650585966074 1109 76 1.7878575469233837 1110 1 -5.331881055945853 1110 2 -1.6660071806295313 1110 3 -1.8370749481911979 1110 4 -1.1727384907657523 1110 5 -1.331867747649781 1110 6 -0.14729829152632945 1110 7 -0.8506462420106367 1110 8 1.4810550536523088 1110 9 -0.36791436446347703 1110 10-11 -0.7518944206244704 1110 12-13 -0.41554109253508287 1110 14-15 -2.2226029571990935 1110 16-17 -0.7713144312395315 1110 18-19 -2.0786214953941275 1110 20-21 -1.3280615053721512 1110 22-23 -0.287103533835694 1110 24-25 -2.611306560384932 1110 26-27 -1.8363185820375847 1110 28-29 -2.1953377319013647 1110 30-31 -1.0108817330470004 1110 32-33 -0.6091629088246044 1110 34-35 0.43489996183838286 1110 36-37 -1.740518217244869 1110 38-39 -0.7270577626154271 1110 40-41 -2.475306729129926 1110 42-43 -2.0305026998240336 1110 44-45 -0.44805982155537905 1110 46-47 -0.5563877846458034 1110 48-49 0.38144794460588116 1110 50-51 -1.9757485958914103 1110 52-53 -3.3586837335334536 1110 54-55 -1.0305993815089067 1110 56-57 -1.1582068220637964 1110 58-59 -2.970147940300084 1110 60-61 -1.2333293876771094 1110 62-63 -1.393324759520528 1110 64-65 0.39699983858497845 1110 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-2.8825294936457446 2117 4 -3.1272839453112056 2117 5 -1.113685929467966 2117 6 -5.2200255642536035 2117 7 -4.7233735147379114 2117 8 -4.7916722190749645 2117 9 -6.304278000827114 2117 10-11 -4.1791671478972 2117 12-13 -5.4700865470805375 2117 14-15 -4.904421139017273 2117 16-17 -3.6258598857849833 2117 18-19 -4.728621495394126 2117 20-21 -2.668970596281241 2117 22-23 -0.6871035338356926 2117 24-25 -0.07039746947584291 2117 26-27 -3.654500400219405 2117 28-29 -4.845337731901363 2117 30-31 -3.2108817330469996 2117 32-33 -2.5500719997336994 2117 34-35 0.2258090527474721 2117 36-37 0.5412999645733123 2117 38-39 -2.3906941262517876 2117 40-41 -0.020761274584472744 2117 42-43 -3.425957245278582 2117 44-45 -2.993514367009926 2117 46-47 -5.188205966463983 2117 48-49 -0.9230975099395735 2117 50-51 -3.080294050436862 2117 52-53 -4.031411006260726 2117 54-55 -3.1351448360543586 2117 56-57 -0.4945704584274324 2117 58-59 -3.0928752130273587 2117 60-61 -2.646965751313477 2117 62-63 -3.643324759520528 2117 64-65 -3.7848183432332014 2117 66-67 -2.9003943053759684 2117 68-69 -3.412150572260092 2117 70-71 -2.1266543668726676 2117 72-73 -3.4183347453023494 2117 74-75 -1.2860436370555632 2117 76 -5.0208381052505295 2118 1 -2.6955174195822167 2118 2 -1.4114617260840774 2118 3 -1.1825294936457453 2118 4 -3.527283945311204 2118 5 -7.713685929467964 2118 6 -3.2200255642536035 2118 7 -3.0233735147379086 2118 8 -6.191672219074963 2118 9 -3.0042780008271137 2118 10-11 -1.6791671478972 2118 12-13 -0.3700865470805361 2118 14-15 -2.3544211390172727 2118 16-17 -3.0258598857849854 2118 18-19 0.02137850460587387 2118 20-21 -0.1189705962812404 2118 22-23 -0.9371035338356926 2118 24-25 -4.420397469475841 2118 26-27 -1.5545004002194034 2118 28-29 -2.5953377319013633 2118 30-31 -3.410881733046999 2118 32-33 -2.650071999733701 2118 34-35 -3.724190947252527 2118 36-37 -4.9087000354266905 2118 38-39 -3.940694126251792 2118 40-41 -3.370761274584474 2118 42-43 -2.8259572452785804 2118 44-45 -2.443514367009925 2118 46-47 -2.7382059664639833 2118 48-49 -0.7230975099395707 2118 50-51 -2.9302940504368635 2118 52-53 -4.631411006260727 2118 54-55 -2.0851448360543614 2118 56-57 -1.0445704584274331 2118 58-59 -1.4928752130273573 2118 60-61 -3.9469657513134777 2118 62-63 0.05667524047947481 2118 64-65 -2.5348183432332014 2118 66-67 -3.6003943053759713 2118 68-69 -1.2621505722600936 2118 70-71 -3.026654366872666 2118 72-73 -2.1183347453023487 2118 74-75 -1.236043637055566 2118 76 -2.8208381052505302 2119 1 2.582260358195562 2119 2 2.788538273915922 2119 3 1.0063593952431447 2119 4 0.7616049435776837 2119 5 0.8752029594209247 2119 6 2.079974435746397 2119 7 3.5432931519287543 2119 8 2.2638833364805926 2119 9 4.440166443617329 2119 10-11 2.2986106298805815 2119 12-13 1.5465801195861317 2119 14-15 4.234467749871612 2119 16-17 1.7185845586594581 2119 18-19 2.754711837939201 2119 20-21 2.6088071814965375 2119 22-23 3.3240075772754203 2119 24-25 2.979602530524158 2119 26-27 4.151055155336152 2119 28-29 4.6657733792097495 2119 30-31 3.266896044730778 2119 32-33 5.7721502224885235 2119 34-35 4.720253497191916 2119 36-37 3.4690777423510895 2119 38-39 3.2981947626370953 2119 40-41 4.062572058748856 2119 42-43 2.6740427547214196 2119 44-45 4.3675967441011885 2119 46-47 3.872905144647131 2119 48-49 2.315791378949317 2119 50-51 2.8752615051186936 2119 52-53 4.42414454929483 2119 54-55 3.820410719501197 2119 56-57 3.0387628749059026 2119 58-59 4.3960136758615285 2119 60-61 1.1530342486865237 2119 62-63 2.9677863515905862 2119 64-65 3.492959434544577 2119 66-67 3.0996056946240316 2119 68-69 4.465627205517688 2119 70-71 2.9900122997939995 2119 72-73 1.90944303247543 2119 74-75 5.558400807388878 2119 76 2.1791618947494698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 10.0 13 42.0 14 130.0 15 181.0 16 249.0 17 314.0 18 315.0 19 279.0 20 226.0 21 175.0 22 146.0 23 123.0 24 76.0 25 77.0 26 89.0 27 83.0 28 75.0 29 107.0 30 122.0 31 177.0 32 200.0 33 309.0 34 407.0 35 643.0 36 1171.0 37 1525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.454242928452576 24.58402662229617 16.38935108153078 31.572379367720465 2 12.629256859230665 22.114986901971598 41.72066731007859 23.53508892871915 3 12.656831655866538 25.410175099958636 29.96001654487798 31.97297669929684 4 10.25782434854543 27.47828484764925 32.68992141182959 29.573969391975734 5 11.112643044257549 40.07996691024404 29.367158417206674 19.44023162829174 6 23.893561284985523 38.61850268854267 15.800358472356265 21.68757755411554 7 19.536743416517304 32.04191369088653 23.038742589273404 25.38260030332276 8 17.179098304150006 41.48628153867365 18.199365779677375 23.135254377498963 9 18.90252309389218 22.15634909692541 19.357507238384116 39.58362057079829 10-11 19.888322073624707 27.822969805597687 25.892734041086445 26.395974079691165 12-13 18.59920033089756 25.499793189025233 26.168482007445192 29.732524472632015 14-15 18.254515372949122 27.457603750172343 23.583344822831933 30.7045360540466 16-17 19.426444229973804 28.429615331586927 24.11415965807252 28.029780780366742 18-19 19.123121466979182 27.361091961946784 24.127947056390457 29.387839514683577 20-21 18.874948297256307 26.988832207362474 24.403695022749208 29.732524472632015 22-23 19.860747276988832 28.539914518130427 23.238659864883495 28.360678339997243 24-25 19.626361505583898 30.518406176754446 22.48724665655591 27.36798566110575 26-27 18.80601130566662 29.498138701227077 24.734592582379705 26.961257410726596 28-29 19.82627878119399 28.870812077760927 25.91341513856335 25.389494002481733 30-31 19.150696263615057 28.209016958499934 25.97545843099407 26.66482834689094 32-33 18.130428788087688 29.884185854129324 25.7272852612712 26.25810009651179 34-35 18.495794843513032 28.643320005514962 25.279194815938233 27.58169033503378 36-37 18.90941679305115 28.567489314766302 26.19605680408107 26.327037088101473 38-39 18.185578381359438 26.919895215772783 27.25768647456225 27.63683992830553 40-41 17.22046049910382 27.52654074176203 26.84406452502413 28.408934234110024 42-43 17.123948710878256 25.796222252860883 28.388253136633118 28.691575899627743 44-45 16.503515786571075 27.62305252998759 28.643320005514962 27.230111677926377 46-47 17.01364952433476 28.146973666069215 27.016407003998346 27.822969805597687 48-49 16.551771680683856 29.780780366744796 26.382186681373227 27.285261271198124 50-51 16.73790155797601 27.967737487936027 26.278781193988692 29.015579760099268 52-53 16.234661519371294 28.477871225699708 26.67172204604991 28.615745208879083 54-55 15.421205018612987 28.69846959878671 25.741072659589136 30.139252723011168 56-57 16.889562939473322 28.160761064387152 25.065490142010205 29.884185854129324 58-59 16.613814973114575 27.333517165310905 26.389080380532192 29.663587481042324 60-61 15.62801599338205 27.457603750172343 25.878946642768508 31.0354336136771 62-63 16.889562939473322 27.27836757203916 25.13442713359989 30.697642354887634 64-65 17.04122432097063 27.05776919895216 23.886667585826554 32.014338894250656 66-67 16.407003998345512 29.822142561698605 22.921549703570935 30.849303736384943 68-69 16.648283468909415 29.539500896180893 22.287329380945817 31.524886253963878 70-71 17.75127533434441 28.608851509720118 22.48724665655591 31.152626499379565 72-73 17.765062732662347 29.194815938232455 21.84613263477182 31.19398869433338 74-75 18.785330208189716 27.595477733351714 22.928443402729908 30.69074865572866 76 17.716806838549566 26.07197021921963 21.935750723838414 34.27547221839239 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 2.5 16 4.5 17 4.0 18 3.5 19 8.0 20 11.5 21 10.0 22 12.5 23 18.0 24 25.0 25 29.0 26 38.5 27 47.0 28 66.0 29 86.0 30 87.0 31 96.5 32 122.0 33 139.0 34 159.5 35 200.0 36 243.0 37 266.0 38 255.5 39 267.5 40 308.5 41 337.5 42 348.0 43 374.0 44 408.0 45 403.5 46 391.0 47 381.0 48 400.5 49 405.0 50 380.0 51 360.5 52 330.0 53 303.5 54 288.0 55 282.5 56 242.5 57 207.5 58 207.0 59 194.5 60 160.0 61 124.5 62 111.0 63 98.5 64 75.5 65 51.0 66 36.5 67 36.0 68 29.0 69 17.5 70 10.0 71 7.0 72 9.0 73 7.5 74 4.0 75 4.0 76 3.0 77 2.5 78 2.5 79 2.0 80 3.5 81 3.5 82 1.5 83 1.0 84 1.0 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5652833310354336 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7253.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.68950778988005 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83196046128501 71.83234523645388 2 10.362438220757825 17.344547083965256 3 2.701812191103789 6.783399972425204 4 0.8072487644151565 2.7023300703157314 5 0.19769357495881384 0.8272438990762443 6 0.08237232289950577 0.41362194953812215 7 0.016474464579901153 0.09651178822556183 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013787398317937405 0.0 2 0.0 0.0 0.0 0.013787398317937405 0.0 3 0.0 0.0 0.0 0.013787398317937405 0.0 4 0.0 0.0 0.0 0.041362194953812215 0.0 5 0.0 0.0 0.0 0.041362194953812215 0.0 6 0.0 0.0 0.0 0.041362194953812215 0.0 7 0.0 0.0 0.0 0.041362194953812215 0.0 8 0.0 0.0 0.0 0.041362194953812215 0.0 9 0.0 0.0 0.0 0.041362194953812215 0.0 10 0.0 0.0 0.0 0.05514959327174962 0.0 11 0.0 0.0 0.0 0.05514959327174962 0.0 12 0.0 0.0 0.0 0.05514959327174962 0.0 13 0.0 0.0 0.0 0.05514959327174962 0.0 14 0.0 0.0 0.0 0.06893699158968702 0.0 15 0.0 0.0 0.0 0.06893699158968702 0.0 16 0.0 0.0 0.0 0.06893699158968702 0.0 17 0.0 0.0 0.0 0.08272438990762443 0.0 18 0.0 0.0 0.0 0.12408658486143664 0.0 19 0.0 0.0 0.0 0.16544877981524886 0.0 20 0.0 0.0 0.0 0.2481731697228733 0.0 21 0.0 0.0 0.0 0.2481731697228733 0.0 22 0.0 0.0 0.0 0.3171101613125603 0.0 23 0.0 0.0 0.0 0.3171101613125603 0.0 24 0.0 0.0 0.0 0.3171101613125603 0.0 25 0.0 0.0 0.0 0.3171101613125603 0.0 26 0.0 0.0 0.0 0.3171101613125603 0.0 27 0.0 0.0 0.0 0.3171101613125603 0.0 28 0.0 0.0 0.0 0.3308975596304977 0.0 29 0.0 0.0 0.0 0.3308975596304977 0.0 30 0.0 0.0 0.0 0.3584723562663725 0.0 31 0.0 0.0 0.0 0.39983455122018474 0.0 32 0.0 0.0 0.0 0.41362194953812215 0.0 33 0.0 0.0 0.0 0.48255894112780917 0.0 34 0.0 0.0 0.0 0.6342203226251206 0.0 35 0.0 0.0 0.0 0.8272438990762443 0.0 36 0.0 0.0 0.0 0.9513304839376809 0.0 37 0.0 0.0 0.0 1.3373776368399284 0.0 38 0.0 0.0 0.0 1.6407003998345513 0.0 39 0.0 0.0 0.0 2.1232593409623606 0.0 40 0.0 0.0 0.0 2.5644560871363575 0.0 41 0.0 0.0 0.0 3.0883772232179787 0.0 42 0.0 0.0 0.0 3.7639597407969116 0.0 43 0.0 0.0 0.0 4.122432097063284 0.0 44 0.0 0.0 0.0 4.742865021370467 0.0 45 0.0 0.0 0.0 5.459809733903213 0.0 46 0.0 0.0 0.0 6.355990624569144 0.0 47 0.0 0.0 0.0 6.976423548876327 0.0 48 0.0 0.0 0.0 7.500344684957948 0.0 49 0.0 0.0 0.0 8.051840617675445 0.0 50 0.0 0.0 0.0 8.630911347028816 0.0 51 0.0 0.0 0.0 9.072108093202813 0.0 52 0.0 0.0 0.0 9.733903212463808 0.0 53 0.0 0.0 0.0 10.340548738453053 0.0 54 0.0 0.0 0.0 10.699021094719427 0.0 55 0.0 0.0 0.0 11.37460361229836 0.0 56 0.0 0.0 0.0 11.89852474837998 0.0 57 0.0 0.0 0.0 12.38108368950779 0.0 58 0.0 0.0 0.0 13.084241003722598 0.0 59 0.0 0.0 0.0 13.566799944850407 0.0 60 0.0 0.0 0.0 13.92527230111678 0.0 61 0.0 0.0 0.0 14.380256445608714 0.0 62 0.0 0.0 0.0 14.959327174962084 0.0 63 0.0 0.0 0.0 15.345374327864333 0.0 64 0.0 0.0 0.0 15.841720667310078 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGGG 15 0.0021382223 69.986206 39 GTTGTGG 15 0.0021382223 69.986206 38 TGTTGTG 20 0.006683237 52.489655 37 TCCACAA 20 0.006683237 52.489655 2 >>END_MODULE