FastQCFastQC Report
Wed 25 May 2016
SRR1780718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4656.411140217840893TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4436.107817454846271No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC831.1443540603888045RNA PCR Primer, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG390.5377085343995588No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC360.49634633944574663No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT280.38604715290224734RNA PCR Primer, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT230.3171101613125603No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT200.2757479663587481No Hit
ACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCT180.24817316972287332RNA PCR Primer, Index 12 (96% over 25bp)
ATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTGCTT140.19302357645112367TruSeq Adapter, Index 12 (96% over 29bp)
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC130.17923617813318626TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTTGAAATCTCGTATGCCGT110.15166138149731145Illumina PCR Primer Index 12 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGTCTT110.15166138149731145No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC110.15166138149731145No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGTCTT110.15166138149731145No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT100.13787398317937405No Hit
CTTATACACATCTCCGAGCCCACGCGACTACTTGAAATCTCGTATGCCGT90.12408658486143666No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT90.12408658486143666TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTAGTATGCCGT80.11029918654349924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATC150.002136551870.068
ACTATCA150.002136551870.069
CTTTACA252.2355707E-455.9999961
AAACACC200.00667803152.566
TGCCGTC1850.037.83783745
CCGTCTT1850.037.83783747
GCCGTCT1850.037.83783746
TCTTCTG1850.037.83783750
TTCTGCT1800.036.94444352
GTCTTCT1900.036.84210249
CGTCTTC1900.036.84210248
TCTCGTA1550.036.12903238
TCTGCTT1750.036.053
TGCTTGA1750.036.055
CTGCTTG1750.036.054
CTCGTAT1750.036.039
TGAAAAA1750.036.059
CTTCTGC1850.035.94594651
GTATGCC1950.035.89743442
TATGCCG1950.035.89743443
Produced by FastQC (version 0.11.5)