##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780718_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7253 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43361367709913 34.0 34.0 34.0 31.0 34.0 2 33.142010202674754 34.0 34.0 34.0 32.0 34.0 3 33.337377636839925 34.0 34.0 34.0 33.0 34.0 4 33.31711016131256 34.0 34.0 34.0 33.0 34.0 5 33.31779953122846 34.0 34.0 34.0 33.0 34.0 6 37.022611333241414 38.0 37.0 38.0 36.0 38.0 7 36.637942920170964 38.0 37.0 38.0 34.0 38.0 8 36.55770026196057 38.0 37.0 38.0 34.0 38.0 9 36.41141596580725 38.0 37.0 38.0 34.0 38.0 10-11 35.99372673376534 38.0 36.5 38.0 32.5 38.0 12-13 35.90590100648008 38.0 36.5 38.0 31.5 38.0 14-15 36.01426995725906 38.0 36.5 38.0 33.0 38.0 16-17 36.154694609127255 38.0 37.0 38.0 33.0 38.0 18-19 36.19123121466979 38.0 37.0 38.0 33.5 38.0 20-21 35.90583206948848 38.0 36.0 38.0 31.5 38.0 22-23 35.89356128498552 38.0 36.5 38.0 32.5 38.0 24-25 35.084378877705774 37.5 35.0 38.0 28.5 38.0 26-27 35.34916586240176 37.0 36.0 38.0 31.0 38.0 28-29 35.15945126154695 37.5 35.5 38.0 29.0 38.0 30-31 34.75713497862954 37.0 34.5 38.0 26.5 38.0 32-33 35.26065076520061 37.0 35.5 38.0 29.0 38.0 34-35 34.858127671308424 37.0 35.0 38.0 27.0 38.0 36-37 35.190610781745484 37.5 36.0 38.0 27.0 38.0 38-39 34.426857851923344 37.0 34.5 38.0 25.5 38.0 40-41 34.32751964704261 37.0 34.0 38.0 25.5 38.0 42-43 35.02474837998069 37.0 35.0 38.0 29.0 38.0 44-45 35.30918240727975 37.5 36.0 38.0 29.0 38.0 46-47 35.46980559768372 38.0 36.0 38.0 31.0 38.0 48-49 35.16813732248725 37.5 35.5 38.0 29.0 38.0 50-51 35.36522818144216 37.5 36.0 38.0 31.0 38.0 52-53 35.141872328691576 37.0 35.5 38.0 29.0 38.0 54-55 35.42858127671308 38.0 36.0 38.0 31.0 38.0 56-57 35.46952984971736 38.0 36.0 38.0 31.0 38.0 58-59 35.488073900454985 38.0 36.0 38.0 31.0 38.0 60-61 35.11147111540052 37.5 35.5 38.0 29.0 38.0 62-63 35.392596167103264 37.5 36.0 38.0 31.0 38.0 64-65 35.468288983868746 38.0 36.0 38.0 31.0 38.0 66-67 34.395491520750035 37.0 34.5 38.0 24.5 38.0 68-69 32.711222942230805 37.0 31.5 38.0 21.0 38.0 70-71 31.776161588308284 37.0 28.5 38.0 15.0 38.0 72-73 30.64276850958224 36.0 24.5 38.0 11.0 38.0 74-75 31.175306769612575 37.0 25.5 38.0 11.0 38.0 76 30.843788777057767 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6582312912918269 1101 2 0.7619663042394293 1101 3 0.12765511165545718 1101 4 0.2920936120454414 1101 5 -0.28265031198220214 1101 6 -2.3938582965349653 1101 7 0.48939729139889465 1101 8 0.7055810622550496 1101 9 -0.3724746468807112 1101 10-11 0.689506078595528 1101 12-13 -0.07789887297361275 1101 14-15 1.2859478002463973 1101 16-17 -0.335529855026671 1101 18-19 -1.5375800955462466 1101 20-21 -1.2156807150185571 1101 22-23 1.4983254721433426 1101 24-25 -2.367091619947672 1101 26-27 -1.1320372947344737 1101 28-29 1.9463946027234513 1101 30-31 2.361064807008404 1101 32-33 0.4837530666790997 1101 34-35 1.1098333422908624 1101 36-37 1.9568507770414953 1101 38-39 0.047566312740507044 1101 40-41 1.5789472068666655 1101 42-43 1.6761559290701271 1101 44-45 1.1003973504612148 1101 46-47 0.009742188844704458 1101 48-49 -3.25653704817082 1101 50-51 -2.74119698606183 1101 52-53 -2.1088811627118673 1101 54-55 -1.2351342350710013 1101 56-57 -2.0482562957411616 1101 58-59 -2.716492242417594 1101 60-61 -2.8471762503454983 1101 62-63 -1.2094094317118262 1101 64-65 -0.44295215738251414 1101 66-67 -3.6651846547964197 1101 68-69 -2.346928446198561 1101 70-71 0.7708644060960168 1101 72-73 -0.9339865707847892 1101 74-75 -0.056908866728168306 1101 76 0.31551649945528837 1102 1 0.3973617260744362 1102 2 0.7184880433698666 1102 3 0.30156815513372237 1102 4 0.9442675250889181 1102 5 0.32604534019171183 1102 6 0.9539677904215509 1102 7 -0.4671244477315426 1102 8 0.9664506274724403 1102 9 1.3666557879018981 1102 10-11 0.9503756438129187 1102 12-13 1.0525359096350897 1102 14-15 -0.40970437366664214 1102 16-17 1.0340353623646337 1102 18-19 0.549376426192886 1102 20-21 1.610406241503192 1102 22-23 1.5418037330129053 1102 24-25 -5.801874228643321 1102 26-27 -1.045080772995341 1102 28-29 1.2507424288104048 1102 30-31 2.3393256765736155 1102 32-33 -0.10320345506004003 1102 34-35 2.3272246466386903 1102 36-37 -1.456192701219372 1102 38-39 2.2866967475231164 1102 40-41 1.665903728605798 1102 42-43 1.7631124508092526 1102 44-45 2.5134408287220893 1102 46-47 2.4662639279751346 1102 48-49 -2.8000153090403828 1102 50-51 0.8022812748077399 1102 52-53 0.8259014459837815 1102 54-55 1.0909527214507406 1102 56-57 1.7995697912153616 1102 58-59 0.9791599314954524 1102 60-61 -3.5863066851281076 1102 62-63 -1.970278996929217 1102 64-65 1.3613956687044393 1102 66-67 1.1609023017253222 1102 68-69 0.08785416249709499 1102 70-71 -0.8813095069474599 1102 72-73 -3.8470300490456566 1102 74-75 0.7909172202283514 1102 76 1.3155164994552884 1103 1 1.540678956348188 1103 2 0.8553640498110866 1103 3 -0.13482475952006467 1103 4 0.9442675250889181 1103 5 0.7608279488873606 1103 6 0.9668502380866073 1103 7 1.4523602543618566 1103 8 1.100106021997398 1103 9 0.7756735012674341 1103 10-11 1.1387814409143644 1103 12-13 -1.672101771524332 1103 14-15 -0.7438428599790399 1103 16-17 -0.3154010305500208 1103 18-19 1.1306968770785488 1103 20-21 -0.35819279231323975 1103 22-23 0.6110468892125809 1103 24-25 -3.985449107870373 1103 26-27 -0.5386395491628164 1103 28-29 -1.038307490674292 1103 30-31 0.9681501532241796 1103 32-33 1.3114503291589656 1103 34-35 2.0494468688609118 1103 36-37 0.04541760473875911 1103 38-39 0.4622200969595127 1103 40-41 -0.2455294436969382 1103 42-43 -0.3665171788203807 1103 44-45 1.309737125018387 1103 46-47 0.9670690809541966 1103 48-49 -1.4417222333560034 1103 50-51 0.11629093664348744 1103 52-53 0.8436148115232385 1103 54-55 0.5780702737856842 1103 56-57 0.8905520778498257 1103 58-59 -0.2905663164916703 1103 60-61 -5.736870292213453 1103 62-63 -1.8100535540950773 1103 64-65 -1.0613096453052222 1103 66-67 -1.3246049446514903 1103 68-69 -0.8356563044916356 1103 70-71 0.5566937136644583 1103 72-73 -5.566031659351616 1103 74-75 -1.3218041968408905 1103 76 -4.1289279449891545 1104 1 0.334753030422263 1104 2 0.8124010868481264 1104 3 0.4459159812206792 1104 4 0.9442675250889181 1104 5 0.7608279488873606 1104 6 0.6009243121606858 1104 7 0.8893972913988932 1104 8 1.4986245405159195 1104 9 1.0275253531192874 1104 10-11 0.6521147742476998 1104 12-13 1.3212315618089931 1104 14-15 1.579860843724667 1104 16-17 1.1053397101907194 1104 18-19 0.4232894696711469 1104 20-21 0.2173627632423134 1104 22-23 0.8591950373607347 1104 24-25 1.5589953365740712 1104 26-27 0.6583974878742183 1104 28-29 1.7646554722886734 1104 30-31 1.5288908939649204 1104 32-33 0.6107095884182314 1104 34-35 0.9072246466386886 1104 36-37 1.4246768639980232 1104 38-39 1.3014793562187705 1104 40-41 1.5537298155623205 1104 42-43 0.2431124508092566 1104 44-45 1.8534408287220927 1104 46-47 1.8166987105838288 1104 48-49 1.3034629518291823 1104 50-51 1.2318464921990468 1104 52-53 0.915466663375085 1104 54-55 0.5839961997116063 1104 56-57 0.4787002259979687 1104 58-59 0.2835077575824059 1104 60-61 1.6023889670458047 1104 62-63 1.126242742201221 1104 64-65 2.263134799139223 1104 66-67 2.2487283886818332 1104 68-69 3.259158510323182 1104 70-71 3.475212232182983 1104 72-73 1.8547090813891245 1104 74-75 0.7100476550109605 1104 76 3.1155164994552855 1105 1 0.47189588756511824 1105 2 0.60668680113384 1105 3 0.6059159812206758 1105 4 0.9442675250889181 1105 5 -0.2748863368269241 1105 6 0.3266385978749682 1105 7 1.20368300568461 1105 8 -0.8099468880555136 1105 9 0.9846682102621429 1105 10-11 1.3292576313905613 1105 12-13 0.42908870466614246 1105 14-15 0.3170037008675237 1105 16-17 -0.9139460040949956 1105 18-19 0.5804323268140053 1105 20-21 9.341918137479865E-4 1105 22-23 -0.24080496263926676 1105 24-25 0.816138193716931 1105 26-27 -0.16231679784006303 1105 28-29 1.0317983294315312 1105 30-31 2.0303194653934895 1105 32-33 0.5707095884182252 1105 34-35 -2.869203924789879 1105 36-37 -1.2846088502876967 1105 38-39 -0.5642349294955125 1105 40-41 1.7280155298480295 1105 42-43 0.12025530795211381 1105 44-45 1.9777265430077975 1105 46-47 1.47169871058383 1105 48-49 -0.4351084767422506 1105 50-51 0.6004179207704752 1105 52-53 0.3026095205179473 1105 54-55 0.36113905685446923 1105 56-57 0.30655736885510976 1105 58-59 -0.19863509956045533 1105 60-61 0.4152461099029452 1105 62-63 -0.8887572577987797 1105 64-65 -0.777579486575064 1105 66-67 -1.2769858970324464 1105 68-69 -1.895841489676819 1105 70-71 -0.42478776781702265 1105 72-73 -0.3967194900394446 1105 74-75 -0.5592380592747546 1105 76 -0.11305492911613868 1106 1 -1.6709612552920206 1106 2 0.1304963249433655 1106 3 0.2725826478873401 1106 4 0.8014103822317722 1106 5 -0.3344101463507343 1106 6 0.040924312160683485 1106 7 -1.3677455657439594 1106 8 0.666243588134968 1106 9 -1.8486651230711857 1106 10-11 0.0673528694858021 1106 12-13 1.0183744189518578 1106 14-15 -1.8020439181800967 1106 16-17 -2.0270412421902293 1106 18-19 0.43757518395685935 1106 20-21 0.8164103822899378 1106 22-23 0.8782426564083536 1106 24-25 -1.4457665681878353 1106 26-27 -0.09684060736387323 1106 28-29 0.37703642466961895 1106 30-31 0.9231766082506354 1106 32-33 -1.5959570782484391 1106 34-35 1.0177008371148801 1106 36-37 1.5665816259027778 1106 38-39 -0.18328254854313286 1106 40-41 2.1327774346099417 1106 42-43 0.3583505460473475 1106 44-45 0.3229646382458995 1106 46-47 0.33479394867907075 1106 48-49 1.6006058089720412 1106 50-51 1.3623226826752344 1106 52-53 -0.4950095271011037 1106 54-55 -0.09124189552648687 1106 56-57 0.25298594028368626 1106 58-59 1.1168410909157345 1106 60-61 1.7128651575219962 1106 62-63 1.1290998850583591 1106 64-65 1.5259919419963595 1106 66-67 0.36587124582469954 1106 68-69 3.788682319846991 1106 70-71 2.860926517897269 1106 72-73 4.508042414722453 1106 74-75 1.387190512153822 1106 76 3.2202784042171935 1107 1 0.5277965086831315 1107 2 0.2837054346742107 1107 3 0.2580898942641525 1107 4 0.6399196990019647 1107 5 0.19561055758300938 1107 6 0.5191851817259021 1107 7 0.5763538131380272 1107 8 -3.207462416005825 1107 9 -2.459431168619844 1107 10-11 0.25472346989987216 1107 12-13 -1.3387684381910034 1107 14-15 -1.12709567801447 1107 16-17 0.7296875362776731 1107 18-19 0.48415903488853473 1107 20-21 0.3712758067205826 1107 22-23 -0.4581962669870947 1107 24-25 -1.2801350982085395 1107 26-27 -0.1102981642996923 1107 28-29 1.0116119940277954 1107 30-31 -0.8345873669046426 1107 32-33 0.09244871885300654 1107 34-35 -0.694514483796091 1107 36-37 1.6742420813893233 1107 38-39 0.5475663127405141 1107 40-41 1.2528602503449306 1107 42-43 1.1326776682005573 1107 44-45 1.6656147417655731 1107 46-47 1.6184378410186113 1107 48-49 1.0043325170465707 1107 50-51 1.6718464921990446 1107 52-53 0.8259014459837815 1107 54-55 1.28660489536378 1107 56-57 0.8430480520849244 1107 58-59 0.7835077575824059 1107 60-61 2.0223889670458064 1107 62-63 0.9862427422012203 1107 64-65 0.7092217556609555 1107 66-67 -2.121706393926857 1107 68-69 -1.194754533155077 1107 70-71 -4.72913559390398 1107 72-73 -2.9992039620891333 1107 74-75 -4.209082779771649 1107 76 -3.119266109240364 1108 1 -0.06524696957773557 1108 2 0.4124010868481278 1108 3 0.20591598122067722 1108 4 0.7442675250889152 1108 5 0.6408279488873632 1108 6 -0.2790756878393168 1108 7 0.24939729139889266 1108 8 0.6586245405159161 1108 9 -0.05247464688071091 1108 10-11 0.9921147742476961 1108 12-13 1.9612315618089937 1108 14-15 1.0398608437246608 1108 16-17 -0.494660289809282 1108 18-19 -0.3967105303288605 1108 20-21 1.2573627632423197 1108 22-23 0.9791950373607321 1108 24-25 1.738995336574071 1108 26-27 -0.26160251212577634 1108 28-29 -0.7753445277113329 1108 30-31 0.3688908939649167 1108 32-33 1.2307095884182218 1108 34-35 0.9272246466386846 1108 36-37 0.9446768639980192 1108 38-39 0.7214793562187722 1108 40-41 0.13372981556232588 1108 42-43 0.7431124508092566 1108 44-45 1.0534408287220884 1108 46-47 1.1766987105838282 1108 48-49 1.503462951829185 1108 50-51 0.5918464921990463 1108 52-53 0.49546666337508327 1108 54-55 0.08399619971160632 1108 56-57 0.07870022599797011 1108 58-59 1.4835077575824087 1108 60-61 1.4823889670458072 1108 62-63 1.9862427422012203 1108 64-65 0.6431347991392187 1108 66-67 2.0287283886818344 1108 68-69 2.1591585103231807 1108 70-71 0.6552122321829756 1108 72-73 -1.3452909186108784 1108 74-75 0.350047655010961 1108 76 1.07551649945529 1109 1 -1.9069861000125172 1109 2 -0.23803369576057065 1109 3 0.12765511165545718 1109 4 -0.36008030099803534 1109 5 0.5869149054091025 1109 6 -0.002553948708879261 1109 7 -0.6845157520793634 1109 8 0.8794941057333077 1109 9 0.8449166574671167 1109 10-11 0.7982017307694349 1109 12-13 1.2264489531133407 1109 14-15 1.1989912785072718 1109 16-17 0.4036005797559383 1109 18-19 0.7450286001059254 1109 20-21 1.6973627632423174 1109 22-23 0.9548472112737798 1109 24-25 -1.410569880817235 1109 26-27 -1.3276894686475131 1109 28-29 -2.07534452771133 1109 30-31 1.2306300243997015 1109 32-33 1.6794052405921391 1109 34-35 -0.020601440317832953 1109 36-37 1.9568507770414953 1109 38-39 1.6127837040448583 1109 40-41 -0.6167049670463811 1109 42-43 0.9370254942875178 1109 44-45 0.38300604611339395 1109 46-47 0.379307406236002 1109 48-49 1.3304194735683055 1109 50-51 -0.8716317686705182 1109 52-53 -1.695837684451 1109 54-55 -3.061221191592736 1109 56-57 0.0821784868675337 1109 58-59 0.8269860184519686 1109 60-61 0.9354324453066738 1109 62-63 -0.3181050838857331 1109 64-65 1.904873929574002 1109 66-67 0.5739457799861825 1109 68-69 1.1748106842362276 1109 70-71 1.0969513626177587 1109 72-73 2.1529699509543434 1109 74-75 1.4430911332718281 1109 76 2.576386064672679 1110 1 -2.248883333214099 1110 2 -0.653053458606422 1110 3 0.1968250721297693 1110 4 0.807903888725285 1110 5 0.7153734034328139 1110 6 0.17728794852431662 1110 7 0.762124564126168 1110 8 0.9822609041522838 1110 9 1.2184344440283823 1110 10-11 -0.5733397712068466 1110 12-13 0.8885042890817232 1110 14-15 1.1871335709973891 1110 16-17 0.7662488010998132 1110 18-19 -0.9585287121470429 1110 20-21 0.12918094506049727 1110 22-23 -1.6498958717301733 1110 24-25 -3.7910046634259302 1110 26-27 1.2483974878742217 1110 28-29 0.5382918359250297 1110 30-31 0.5952545303285603 1110 32-33 0.9570732247818654 1110 34-35 1.7590428284568773 1110 36-37 2.2819495912707453 1110 38-39 1.8123884471278657 1110 40-41 -0.8044520026194988 1110 42-43 -0.12325118555438763 1110 44-45 0.28616810144936267 1110 46-47 1.2785168924020098 1110 48-49 1.9025538609200865 1110 50-51 0.37639194674449783 1110 52-53 1.5591030270114459 1110 54-55 0.503996199711608 1110 56-57 -0.393117955820216 1110 58-59 -0.398310424235774 1110 60-61 0.09057078522762652 1110 62-63 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0.1291585103231867 2116 70-71 2.3252122321829773 2116 72-73 1.5247090813891262 2116 74-75 0.7300476550109636 2116 76 0.9155164994552898 2117 1 0.9147530304222613 2117 2 0.8924010868481247 2117 3 0.6059159812206758 2117 4 0.24426752508891525 2117 5 0.7608279488873606 2117 6 0.9409243121606821 2117 7 1.4893972913988947 2117 8 1.118624540515917 2117 9 -0.8724746468807112 2117 10-11 0.6221147742477058 2117 12-13 0.06123156180899514 2117 14-15 0.6098608437246611 2117 16-17 -0.0746602898092803 2117 18-19 -0.07671053032885311 2117 20-21 -0.5526372367576826 2117 22-23 0.4091950373607389 2117 24-25 -2.0410046634259302 2117 26-27 -1.4516025121257812 2117 28-29 -4.675344527711328 2117 30-31 0.7088908939649201 2117 32-33 -3.929290411581775 2117 34-35 -1.3227753533613082 2117 36-37 -4.59532313600198 2117 38-39 1.8214793562187737 2117 40-41 1.4637298155623242 2117 42-43 1.863112450809254 2117 44-45 -0.13655917127790929 2117 46-47 1.396698710583827 2117 48-49 0.49346295182918 2117 50-51 -2.3281535078009554 2117 52-53 -0.604533336624911 2117 54-55 0.45399619971161087 2117 56-57 -0.3612997740020276 2117 58-59 -1.4664922424175941 2117 60-61 -3.827611032954195 2117 62-63 1.136242742201219 2117 64-65 -2.766865200860778 2117 66-67 -0.9912716113181617 2117 68-69 -5.170841489676818 2117 70-71 -2.5747877678170212 2117 72-73 -4.225290918610874 2117 74-75 -2.4699523449890393 2117 76 -3.08448350054471 2118 1 -0.9852469695777373 2118 2 -0.40759891315187247 2118 3 -1.5940840187793235 2118 4 0.7442675250889152 2118 5 0.7608279488873606 2118 6 0.34092431216068064 2118 7 1.2893972913988918 2118 8 0.41862454051591413 2118 9 -1.8724746468807112 2118 10-11 -1.3778852257522942 2118 12-13 -1.1887684381910049 2118 14-15 1.4598608437246625 2118 16-17 -4.224660289809279 2118 18-19 -1.826710530328853 2118 20-21 -4.902637236757684 2118 22-23 -2.790804962639264 2118 24-25 -0.24100466342593307 2118 26-27 -3.3016025121257755 2118 28-29 -0.82534452771133 2118 30-31 -6.1911091060350785 2118 32-33 -3.8792904115817777 2118 34-35 -0.9727753533613068 2118 36-37 -3.8453231360019835 2118 38-39 -4.628520643781229 2118 40-41 -1.8862701844376772 2118 42-43 -0.5368875491907446 2118 44-45 -5.286559171277908 2118 46-47 -2.5533012894161686 2118 48-49 -3.1565370481708257 2118 50-51 -3.978153507800954 2118 52-53 -3.9045333366249153 2118 54-55 -1.1960038002883948 2118 56-57 -1.5612997740020305 2118 58-59 -3.166492242417597 2118 60-61 -4.127611032954192 2118 62-63 -3.4637572577987825 2118 64-65 -0.9668652008607808 2118 66-67 -3.1412716113181602 2118 68-69 -6.220841489676818 2118 70-71 -7.224787767817023 2118 72-73 0.07470908138912336 2118 74-75 -1.3699523449890378 2118 76 1.3155164994552884 2119 1 0.8369752526444856 2119 2 -0.6631544687074324 2119 3 0.49480487010956153 2119 4 -0.611288030466639 2119 5 0.4274946155540249 2119 6 -0.9590756878393165 2119 7 0.044952846954451786 2119 8 0.9519578738492527 2119 9 0.7386364642304031 2119 10-11 -1.1389963368634142 2119 12-13 -0.8943239937465606 2119 14-15 0.6265275103913339 2119 16-17 0.9808952657462768 2119 18-19 0.1677339141155869 2119 20-21 -0.8581927923132397 2119 22-23 -2.796360518194824 2119 24-25 -2.0410046634259302 2119 26-27 0.9428419323186645 2119 28-29 0.25798880562199855 2119 30-31 0.9311131161871486 2119 32-33 2.2373762550848895 2119 34-35 -0.6172197978057525 2119 36-37 1.3046768639980186 2119 38-39 0.610368245107658 2119 40-41 -1.9862701844376787 2119 42-43 2.040890228587031 2119 44-45 -0.04211472683346784 2119 46-47 0.9855875994727157 2119 48-49 0.5212407296069586 2119 50-51 0.11629093664348744 2119 52-53 0.3621333300417575 2119 54-55 0.2817739774893866 2119 56-57 0.6498113371090852 2119 58-59 -0.3276033535287084 2119 60-61 1.5223889670458064 2119 62-63 0.2640205199789989 2119 64-65 2.049801465805892 2119 66-67 1.842061722015167 2119 68-69 -0.13195260078793325 2119 70-71 2.408545565516313 2119 72-73 3.1747090813891248 2119 74-75 2.696714321677625 2119 76 2.8710720550108455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 6.0 24 6.0 25 21.0 26 24.0 27 67.0 28 95.0 29 159.0 30 246.0 31 371.0 32 525.0 33 703.0 34 1014.0 35 1088.0 36 1369.0 37 1558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.492072245967186 26.88542671997794 10.905832069488488 34.71666896456639 2 12.505170274369226 16.999862126016822 51.50972011581415 18.985247483799807 3 13.677099131393906 28.650213704673927 36.35736936440094 21.31531779953123 4 10.699021094719427 25.561836481455952 32.359023852199094 31.38011857162553 5 10.464635323314491 39.55604577416242 37.35006204329243 12.629256859230665 6 21.5634909692541 40.217840893423414 15.09720115814146 23.12146697918103 7 19.991727561009238 35.94374741486281 18.089066593133875 25.97545843099407 8 17.316972287329378 39.32166000275748 24.96897835378464 18.3923893561285 9 17.04122432097063 15.014476768233834 18.874948297256307 49.06935061353923 10-11 19.171377361091963 26.389080380532192 30.807941541431134 23.63160071694471 12-13 22.983592996001654 18.633668826692404 27.602371432510687 30.780366744795256 14-15 21.942644422997382 23.831517992554804 20.21232593409624 34.01351165035158 16-17 24.189990348821176 27.423135254377502 19.784916586240175 28.601957810561146 18-19 23.735006204329242 26.20295050324004 21.039569833172482 29.022473459258237 20-21 22.05294360954088 23.155935474975873 19.66772370053771 35.12339721494554 22-23 21.811664138976976 30.911347028815662 20.922376947470013 26.35461188473735 24-25 23.05942368675031 32.91741348407555 20.922376947470013 23.100785881704123 26-27 22.128774300289535 33.000137873983185 25.320557010892042 19.55053081483524 28-29 17.075692816765475 23.149041775816904 31.531779953122847 28.243485454294774 30-31 20.122707845029645 27.037088101475256 28.098717771956434 24.741486281538673 32-33 18.158003584723563 30.64249276161588 32.483110437060525 18.71639321660003 34-35 22.728526127119814 33.11043706052668 27.023300703157314 17.137736109196194 36-37 17.454846270508757 33.75155108231077 26.630359851096095 22.16324279608438 38-39 20.7707155659727 22.976699296842686 27.816076106438715 28.4365090307459 40-41 16.90335033779126 26.216737901557973 32.77264580173721 24.107265958913555 42-43 26.223631600716946 21.287743002895354 33.62057079829036 18.868054598097338 44-45 17.46863366882669 25.851371846132636 29.31200882393492 27.36798566110575 46-47 25.623879773886664 20.646628981111263 27.85054460223356 25.878946642768508 48-49 21.694471253274507 19.7573417896043 28.22280435681787 30.325382600303325 50-51 16.517303184889013 20.322625120639735 33.75155108231077 29.408520612160487 52-53 18.9645663863229 20.963739142423822 39.673238659864886 20.39845581138839 54-55 16.17261822694058 20.97752654074176 34.72356266372535 28.12629256859231 56-57 24.679442989107955 21.556597270095132 28.17454846270509 25.589411278091823 58-59 15.931338756376674 30.608024265821037 33.179374052116366 20.281262925685922 60-61 19.784916586240175 37.49482972563077 23.031848890114436 19.688404798014613 62-63 14.587067420377775 44.285123397214946 21.542809871777198 19.58499931063008 64-65 13.677099131393906 44.33337929132773 20.570798290362607 21.418723286915757 66-67 14.414724941403556 44.05073762581001 20.50875499793189 21.02578243485454 68-69 14.614642217013648 41.27257686474562 21.1016131256032 23.01116779263753 70-71 15.207500344684957 37.83262098442024 21.15676271887495 25.803115952019855 72-73 16.2553426168482 35.06135392251482 21.591065765889976 27.092237694747002 74-75 15.243019648397105 35.47052740434333 22.30954843157532 26.976904515684247 76 15.593547497587204 36.343581966083 21.701364952433476 26.361505583896317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 4.0 19 7.0 20 13.5 21 20.0 22 18.5 23 18.0 24 26.0 25 33.0 26 41.5 27 52.5 28 53.5 29 52.0 30 68.5 31 89.5 32 107.0 33 120.0 34 123.0 35 155.5 36 234.5 37 284.0 38 381.0 39 563.0 40 677.5 41 649.0 42 591.0 43 543.5 44 443.5 45 370.5 46 350.0 47 336.5 48 312.5 49 272.0 50 242.0 51 239.0 52 225.5 53 218.5 54 222.0 55 203.0 56 175.0 57 145.0 58 124.0 59 121.5 60 104.5 61 74.5 62 59.0 63 60.5 64 47.0 65 30.0 66 24.0 67 20.0 68 14.5 69 5.5 70 2.0 71 2.0 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0068936991589687024 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.705087549979325 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28671943711521 48.46270508754998 2 15.721196130167106 19.71597959465049 3 4.441512752858399 8.355163380670067 4 1.2752858399296394 3.198676409761478 5 0.6156552330694811 1.930235764511237 6 0.1978891820580475 0.7445195091686199 7 0.04397537379067722 0.19302357645112367 8 0.02198768689533861 0.11029918654349924 9 0.04397537379067722 0.24817316972287332 >10 0.2858399296394019 3.3779125878946643 >50 0.02198768689533861 1.1443540603888045 >100 0.04397537379067722 12.518957672687165 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 465 6.411140217840893 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 443 6.107817454846271 No Hit CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 83 1.1443540603888045 RNA PCR Primer, Index 12 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 39 0.5377085343995588 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 36 0.49634633944574663 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT 28 0.38604715290224734 RNA PCR Primer, Index 12 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT 23 0.3171101613125603 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT 20 0.2757479663587481 No Hit ACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCT 18 0.24817316972287332 RNA PCR Primer, Index 12 (96% over 25bp) ATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTGCTT 14 0.19302357645112367 TruSeq Adapter, Index 12 (96% over 29bp) CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 13 0.17923617813318626 TruSeq Adapter, Index 12 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTCCTTGAAATCTCGTATGCCGT 11 0.15166138149731145 Illumina PCR Primer Index 12 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGTCTT 11 0.15166138149731145 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 11 0.15166138149731145 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGTCTT 11 0.15166138149731145 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 10 0.13787398317937405 No Hit CTTATACACATCTCCGAGCCCACGCGACTACTTGAAATCTCGTATGCCGT 9 0.12408658486143666 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT 9 0.12408658486143666 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTAGTATGCCGT 8 0.11029918654349924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013787398317937405 0.0 2 0.0 0.0 0.0 0.013787398317937405 0.0 3 0.0 0.0 0.0 0.013787398317937405 0.0 4 0.0 0.0 0.0 0.05514959327174962 0.0 5 0.0 0.0 0.0 0.05514959327174962 0.0 6 0.0 0.0 0.0 0.05514959327174962 0.0 7 0.0 0.0 0.0 0.05514959327174962 0.0 8 0.0 0.0 0.0 0.05514959327174962 0.0 9 0.0 0.0 0.0 0.06893699158968702 0.0 10 0.0 0.0 0.0 0.06893699158968702 0.0 11 0.0 0.0 0.0 0.06893699158968702 0.0 12 0.0 0.0 0.0 0.06893699158968702 0.0 13 0.0 0.0 0.0 0.06893699158968702 0.0 14 0.0 0.0 0.0 0.08272438990762443 0.0 15 0.0 0.0 0.0 0.08272438990762443 0.0 16 0.0 0.0 0.0 0.08272438990762443 0.0 17 0.0 0.0 0.0 0.08272438990762443 0.0 18 0.0 0.0 0.0 0.12408658486143664 0.0 19 0.0 0.0 0.0 0.19302357645112367 0.0 20 0.0 0.0 0.0 0.2619605680408107 0.0 21 0.0 0.0 0.0 0.2619605680408107 0.0 22 0.0 0.0 0.0 0.2619605680408107 0.0 23 0.0 0.0 0.0 0.2619605680408107 0.0 24 0.0 0.0 0.0 0.2757479663587481 0.0 25 0.0 0.0 0.0 0.2757479663587481 0.0 26 0.0 0.0 0.0 0.2757479663587481 0.0 27 0.0 0.0 0.0 0.2757479663587481 0.0 28 0.0 0.0 0.0 0.2895353646766855 0.0 29 0.0 0.0 0.0 0.3033227629946229 0.0 30 0.0 0.0 0.0 0.3171101613125603 0.0 31 0.0 0.0 0.0 0.3446849579484351 0.0 32 0.0 0.0 0.0 0.3446849579484351 0.0 33 0.0 0.0 0.0 0.41362194953812215 0.0 34 0.0 0.0 0.0 0.5239211360816214 0.0 35 0.0 0.0 0.0 0.7031573142148076 0.0 36 0.0 0.0 0.0 0.8134565007583069 0.0 37 0.0 0.0 0.0 1.2408658486143664 0.0 38 0.0 0.0 0.0 1.461464221701365 0.0 39 0.0 0.0 0.0 1.9026609678753619 0.0 40 0.0 0.0 0.0 2.3024955190955465 0.0 41 0.0 0.0 0.0 2.8126292568592306 0.0 42 0.0 0.0 0.0 3.433062181166414 0.0 43 0.0 0.0 0.0 3.8604715290224734 0.0 44 0.0 0.0 0.0 4.494691851647594 0.0 45 0.0 0.0 0.0 5.170274369226527 0.0 46 0.0 0.0 0.0 6.038880463256583 0.0 47 0.0 0.0 0.0 6.686888184199642 0.0 48 0.0 0.0 0.0 7.210809320281263 0.0 49 0.0 0.0 0.0 7.8174548462705085 0.0 50 0.0 0.0 0.0 8.45167516889563 0.0 51 0.0 0.0 0.0 8.865297118433752 0.0 52 0.0 0.0 0.0 9.582241830966497 0.0 53 0.0 0.0 0.0 10.216462153591618 0.0 54 0.0 0.0 0.0 10.57493450985799 0.0 55 0.0 0.0 0.0 11.250517027436922 0.0 56 0.0 0.0 0.0 11.760650765200607 0.0 57 0.0 0.0 0.0 12.270784502964291 0.0 58 0.0 0.0 0.0 13.029091410450848 0.0 59 0.0 0.0 0.0 13.49786295326072 0.0 60 0.0 0.0 0.0 13.856335309527092 0.0 61 0.0 0.0 0.0 14.297532055701089 0.0 62 0.0 0.0 0.0 14.835240590100648 0.0 63 0.0 0.0 0.0 15.19371294636702 0.0 64 0.0 0.0 0.0 15.662484489176892 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTATC 15 0.0021365518 70.0 68 ACTATCA 15 0.0021365518 70.0 69 CTTTACA 25 2.2355707E-4 55.999996 1 AAACACC 20 0.006678031 52.5 66 TGCCGTC 185 0.0 37.837837 45 CCGTCTT 185 0.0 37.837837 47 GCCGTCT 185 0.0 37.837837 46 TCTTCTG 185 0.0 37.837837 50 TTCTGCT 180 0.0 36.944443 52 GTCTTCT 190 0.0 36.842102 49 CGTCTTC 190 0.0 36.842102 48 TCTCGTA 155 0.0 36.129032 38 TCTGCTT 175 0.0 36.0 53 TGCTTGA 175 0.0 36.0 55 CTGCTTG 175 0.0 36.0 54 CTCGTAT 175 0.0 36.0 39 TGAAAAA 175 0.0 36.0 59 CTTCTGC 185 0.0 35.945946 51 GTATGCC 195 0.0 35.897434 42 TATGCCG 195 0.0 35.897434 43 >>END_MODULE