FastQCFastQC Report
Wed 25 May 2016
SRR1780717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109362
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAC207.836044E-443.9876481
TTTAGTG254.435668E-543.9675379
TTCGTCC254.435668E-543.9675372
CTTTCGC207.8537286E-443.96753731
GTATGGT301.2851051E-436.7067520
TGGTTAT301.2955428E-436.65637623
GTTCGTC301.2955428E-436.6563761
ATGGTTA301.2955428E-436.65637622
CCTTTAG301.2990375E-436.6396147
CTTTAGT301.2990375E-436.6396148
TCGGAGA250.002330930735.2384813
AACAATA250.002336178235.22234321
GTATTCT250.002351977435.1740331
GTGGACT250.002351977435.174034
TCGGTGG756.002665E-1132.2428642
GGTTATT353.204168E-431.41974824
AGTCTGT353.2127826E-431.4053842
TATACAG353.2127826E-431.405385
CGGTGGT502.3491084E-630.77727543
TTGTGTA453.9811428E-529.39232816
Produced by FastQC (version 0.11.5)