FastQCFastQC Report
Wed 25 May 2016
SRR1780717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109362
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1332612.1852197289735No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT57805.2851996122967755TruSeq Adapter, Index 7 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC18831.7218046487811123No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG14871.3597044677310217No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC6360.5815548362319636No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT3730.3410691099284944No Hit
ATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTTCTGCTT2580.23591375432051354TruSeq Adapter, Index 7 (96% over 27bp)
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2310.2112251056125528No Hit
CATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTTCTGCT2090.19110842888754778TruSeq Adapter, Index 7 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTTCT1940.1773925129386807RNA PCR Primer, Index 7 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1240.11338490517730107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGG207.810012E-444.0173845
GCACAGT207.810012E-444.0173846
ATTTCGT207.810012E-444.0173846
TTTCGTG207.8276376E-443.9972537
CGTGACC207.8276376E-443.99725331
TTCGTGC207.8276376E-443.9972538
CCGTCTT6750.038.4568644
GCCGTCT7350.035.31752843
GTGTTGT250.002338935435.2139054
GTACACT250.002338935435.2139056
AGTGTAG250.002338935435.2139051
GGTAATC250.002344193635.19780310
CTAAGCC250.002344193635.19780342
TACATCT250.002344193635.19780337
CCTTTGC250.002344193635.19780338
CACAGTC250.002344193635.1978037
AGACAAC250.002344193635.19780335
CGCAGCC250.002344193635.19780313
AAGGGAC250.002344193635.19780310
GCCAGTG250.002344193635.19780326
Produced by FastQC (version 0.11.5)