##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780716_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7149 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.603161281298085 23.0 12.0 31.0 12.0 33.0 2 20.85340607077913 21.0 11.0 31.0 11.0 34.0 3 21.054972723457826 23.0 11.0 31.0 11.0 34.0 4 20.331375017484962 21.0 11.0 31.0 11.0 34.0 5 20.05427332494055 21.0 11.0 31.0 11.0 34.0 6 20.590991747097497 21.0 11.0 31.0 11.0 36.0 7 20.736186879283817 21.0 11.0 31.0 11.0 36.0 8 20.971184781088265 21.0 11.0 31.0 11.0 36.0 9 22.01286893271786 21.0 12.0 33.0 12.0 37.0 10-11 21.205553224227163 21.0 11.0 31.0 11.0 36.5 12-13 21.25101412785005 21.0 11.0 31.0 11.0 36.0 14-15 20.870471394600642 21.0 11.0 31.0 11.0 36.0 16-17 20.50867254161421 21.0 11.0 31.0 11.0 36.0 18-19 20.8317247167436 21.0 11.0 31.0 11.0 36.0 20-21 20.966778570429433 21.0 11.0 29.5 11.0 35.5 22-23 20.22345782626941 16.0 11.0 29.0 11.0 35.5 24-25 20.425164358651557 16.0 11.0 29.0 10.5 36.0 26-27 20.17862638131207 21.0 11.0 28.0 10.5 36.0 28-29 19.69527206602322 11.0 11.0 27.0 11.0 35.0 30-31 19.674010351098055 11.0 11.0 27.0 11.0 34.5 32-33 19.923835501468737 16.0 11.0 27.0 11.0 35.0 34-35 20.458735487480766 21.0 11.0 27.0 11.0 35.0 36-37 20.122884319485244 16.0 11.0 27.0 11.0 34.5 38-39 19.309343964190795 11.0 11.0 26.5 11.0 34.0 40-41 19.77094698559239 16.0 11.0 27.0 11.0 34.0 42-43 19.85837180025178 16.0 11.0 27.0 11.0 34.0 44-45 19.751993285774233 11.0 11.0 27.0 11.0 34.0 46-47 19.891872989229263 16.0 11.0 27.0 11.0 34.0 48-49 20.120016785564417 21.0 11.0 27.0 11.0 34.0 50-51 20.32480067142258 21.0 11.0 27.0 11.0 34.5 52-53 20.417960553923628 21.0 11.0 27.0 11.0 34.0 54-55 20.40495174150231 21.0 11.0 27.0 11.0 34.0 56-57 19.545530843474612 16.0 11.0 26.5 11.0 34.0 58-59 19.78129808364806 16.0 11.0 26.5 11.0 34.0 60-61 20.00846272205903 20.0 11.0 27.0 11.0 34.0 62-63 19.533431249125755 15.0 11.0 26.0 11.0 34.0 64-65 19.718352217093297 15.5 11.0 26.5 11.0 34.0 66-67 20.115540635053854 19.0 11.0 27.0 11.0 34.0 68-69 19.632396139320186 19.0 11.0 26.5 10.5 34.0 70-71 19.46600923206043 18.5 11.0 26.0 11.0 34.0 72-73 19.644146034410404 19.0 11.0 26.5 11.0 34.0 74-75 19.192824171212756 19.0 11.0 25.0 11.0 33.5 76 19.101133025597985 19.0 11.0 25.0 11.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.573328866130513 1101 2 0.8104862452603605 1101 3 0.9404331777749171 1101 4 -3.89086708004848 1101 5 -2.9764025801611353 1101 6 -6.209151602227008 1101 7 -3.412702553543358 1101 8 -1.9204927511429055 1101 9 1.1274591957930724 1101 10-11 -1.039278998989257 1101 12-13 -0.7664972770290959 1101 14-15 -1.8695957015844513 1101 16-17 -0.9376040015829368 1101 18-19 -3.350857912410582 1101 20-21 -1.7206058399442838 1101 22-23 -3.1461182968738584 1101 24-25 -0.43197220022613436 1101 26-27 1.3487267755509542 1101 28-29 -1.4887143048481022 1101 30-31 -1.729241011441804 1101 32-33 -1.252063411915337 1101 34-35 -2.3943977657506217 1101 36-37 -1.9381278348934607 1101 38-39 -0.9790661777773764 1101 40-41 -0.6703443217406644 1101 42-43 -1.7750015861535182 1101 44-45 -2.4899663813749378 1101 46-47 -2.1662420708828733 1101 48-49 -0.6282376517282628 1101 50-51 -2.0229262172296245 1101 52-53 -1.610186246366382 1101 54-55 -1.5115376126123508 1101 56-57 1.3139531432936238 1101 58-59 0.060798608720226355 1101 60-61 -0.6180689957684997 1101 62-63 -0.3905169503585846 1101 64-65 -1.9667624664026597 1101 66-67 -0.8813721311978817 1101 68-69 -1.236907614037417 1101 70-71 -0.9686478586682234 1101 72-73 -0.6267986447043796 1101 74-75 -0.2873302925845884 1101 76 1.6631472341727758 1102 1 -3.2921682098928784 1102 2 -0.25384124011975473 1102 3 0.18019925964626538 1102 4 3.1179048497760817 1102 5 -0.3126598901026547 1102 6 0.23529284221743652 1102 7 -0.6320007991573959 1102 8 -1.5608436283358884 1102 9 -0.6941782311075109 1102 10-11 -0.42524391126996264 1102 12-13 0.7598185124445891 1102 14-15 0.2839130703453705 1102 16-17 -0.48146365070574504 1102 18-19 1.1155163565952684 1102 20-21 0.3071719378334947 1102 22-23 -0.4209720980434497 1102 24-25 1.1747529459726955 1102 26-27 -0.6600451542736074 1102 28-29 0.8475430050934136 1102 30-31 -2.264328730740047 1102 32-33 0.031562319078815904 1102 34-35 0.10121626933709393 1102 36-37 -0.7816950863554517 1102 38-39 0.2855537052635597 1102 40-41 1.1147433975575787 1102 42-43 -2.959212112469306 1102 44-45 -0.7706681357609035 1102 46-47 -1.3899262814091884 1102 48-49 1.307434862891622 1102 50-51 2.9653778763376266 1102 52-53 5.271392701002039 1102 54-55 1.0586378259841425 1102 56-57 0.6736022661006409 1102 58-59 0.8926699537494684 1102 60-61 0.07637544867594315 1102 62-63 -2.429990634569112 1102 64-65 -2.4828443377476894 1102 66-67 -4.80242476277683 1102 68-69 -0.5512350994175321 1102 70-71 -0.17186423293722797 1102 72-73 -0.6765062470435623 1102 74-75 -0.5680320469705542 1102 76 -1.3894843447745941 1103 1 0.29555108835273813 1103 2 -0.12006931029519308 1103 3 -0.5040112666695258 1103 4 -2.51586708004848 1103 5 -1.8236248023833568 1103 6 -3.0563738244492313 1103 7 -0.8710358868766939 1103 8 -2.1704927511429055 1103 9 -1.7336519153180383 1103 10-11 -0.6851123323225927 1103 12-13 -2.6623306103624316 1103 14-15 -0.577929034917787 1103 16-17 -0.4167706682496046 1103 18-19 -0.15641346796613576 1103 20-21 0.286338604500159 1103 22-23 -1.1183405190960798 1103 24-25 -0.5500277557816879 1103 26-27 -1.25543989111571 1103 28-29 -2.620658749292552 1103 30-31 -1.3959076781084683 1103 32-33 -1.8006745230264478 1103 34-35 1.3764355675827105 1103 36-37 2.7840943873287607 1103 38-39 -0.6387883999995978 1103 40-41 -0.19117765507400009 1103 42-43 -1.0250015861535182 1103 44-45 -2.3649663813749378 1103 46-47 -2.312075404216209 1103 48-49 -1.8296265406171486 1103 50-51 -0.682648439451846 1103 52-53 0.8898137536336179 1103 54-55 -0.053204279279015054 1103 56-57 -2.248546856706376 1103 58-59 -0.7586458357242165 1103 60-61 -0.444457884657389 1103 62-63 -0.4530169503585846 1103 64-65 -0.08481802195821686 1103 66-67 0.32696120213545043 1103 68-69 -3.042463169592974 1103 70-71 -2.621425636446002 1103 72-73 -1.4462430891488225 1103 74-75 -0.5581636259179241 1103 76 -3.3368527658272242 1104 1 -2.35111557831393 1104 2 -2.251735976961861 1104 3 0.3359887333304741 1104 4 -2.524200413381813 1104 5 -2.405291469050024 1104 6 -0.6047071577825633 1104 7 -2.739369220210026 1104 8 -4.687159417809575 1104 9 -3.9236519153180396 1104 10-11 -0.5526123323225924 1104 12-13 -2.3264972770290946 1104 14-15 0.5870709650822121 1104 16-17 -4.050937334916274 1104 18-19 0.2902531987005297 1104 20-21 -1.4128280621665041 1104 22-23 -2.428340519096082 1104 24-25 -3.3641944224483566 1104 26-27 -2.037939891115709 1104 28-29 1.5601745840407837 1104 30-31 0.26409232189153187 1104 32-33 -1.7831745230264495 1104 34-35 0.0022689009160394846 1104 36-37 -1.405905612671237 1104 38-39 -0.4512883999995978 1104 40-41 0.30632234492599864 1104 42-43 0.7049984138464822 1104 44-45 -0.5832997147082715 1104 46-47 -0.5762420708828735 1104 48-49 0.2895401260495163 1104 50-51 -1.8051484394518447 1104 52-53 -0.4601862463663835 1104 54-55 1.101795720720986 1104 56-57 0.5472864766269545 1104 58-59 -0.6536458357242161 1104 60-61 0.6963754486759406 1104 62-63 -0.120516950358585 1104 64-65 -0.8523180219582152 1104 66-67 0.9586278688021181 1104 68-69 -1.4291298362596372 1104 70-71 0.5502410302206648 1104 72-73 -1.2712430891488218 1104 74-75 -0.4406636259179244 1104 76 -0.1768527658272241 1105 1 0.35965365245530023 1105 2 -0.14250520773109088 1105 3 -0.23478049743875573 1105 4 1.3911842020028047 1105 5 0.32701622325766877 1105 6 -1.2262456193210234 1105 7 -0.8870615279023326 1105 8 0.41284058219042663 1105 9 -0.4451903768564982 1105 10-11 1.3389261292158672 1105 12-13 2.2719642614324442 1105 14-15 4.35476327277452 1105 16-17 0.6906011266221874 1105 18-19 2.7217916602389884 1105 20-21 -0.6735972929357352 1105 22-23 2.0162748655193035 1105 24-25 0.8265748083208742 1105 26-27 2.70129087811506 1105 28-29 -0.3690561851899865 1105 30-31 -1.472830755031545 1105 32-33 1.502210092358169 1105 34-35 0.9838073624545025 1105 36-37 3.7840943873287607 1105 38-39 0.8756346769234788 1105 40-41 2.9193992680029233 1105 42-43 2.0519214907695584 1105 44-45 1.4074695160609565 1105 46-47 2.5837579291171267 1105 48-49 0.9956939722033624 1105 50-51 -0.43745613175953935 1105 52-53 -0.2640324002125354 1105 54-55 1.1487187976440616 1105 56-57 2.628055707396186 1105 58-59 3.789431087352707 1105 60-61 0.5571446794451766 1105 62-63 -0.46744002728166123 1105 64-65 0.6507589011187065 1105 66-67 1.195550945725195 1105 68-69 0.6370240098942084 1105 70-71 0.6702410302206658 1105 72-73 1.4672184493127176 1105 74-75 2.7062594510051525 1105 76 4.0093010803266225 1106 1 0.6526939454955958 1106 2 -1.840307405533288 1106 3 -1.5516303142885732 1106 4 0.13294244376104558 1106 5 -0.4129105166690721 1106 6 1.9495785565031518 1106 7 1.9444403035994995 1106 8 -0.37287370352385807 1106 9 -0.6741281057942281 1106 10-11 4.770244810534553 1106 12-13 4.495407484875663 1106 14-15 0.11849953651078238 1106 16-17 0.8004912365122969 1106 18-19 -0.7754610870137562 1106 20-21 2.2833624140239692 1106 22-23 1.0126118618562998 1106 24-25 0.4767579585040238 1106 26-27 2.2534886803128593 1106 28-29 1.471603155469353 1106 30-31 0.10409232189153172 1106 32-33 2.898730238878315 1106 34-35 4.307983186630327 1106 36-37 1.5340943873287607 1106 38-39 0.3344258857146869 1106 40-41 1.8415604401640948 1106 42-43 1.7488079376560073 1106 44-45 3.3433669519583944 1106 46-47 -0.44005159469239885 1106 48-49 1.411444887954275 1106 50-51 -0.12610082040422554 1106 52-53 2.877908991728855 1106 54-55 0.012271911197174745 1106 56-57 -1.8050944757539966 1106 58-59 0.6044494023710207 1106 60-61 1.0049468772473737 1106 62-63 -0.06908837893001518 1106 64-65 2.084824835184641 1106 66-67 3.0233897735640234 1106 68-69 3.338489211359409 1106 70-71 -0.3773780173983816 1106 72-73 3.1668521489464148 1106 74-75 3.1650506597963606 1106 76 4.13933771036325 1107 1 0.8596536524553002 1107 2 0.5113409461150624 1107 3 -0.6578574205156791 1107 4 2.737338048156648 1107 5 0.6731700694115155 1107 6 0.31221591914051317 1107 7 -0.6947538355946428 1107 8 -0.3563901870403434 1107 9 -0.022113453779578407 1107 10-11 -0.8149200246302861 1107 12-13 -0.6511126616444827 1107 14-15 2.5278401958514465 1107 16-17 1.3252165112375742 1107 18-19 0.9333301217774519 1107 20-21 1.9033257839873414 1107 22-23 -1.1183405190960798 1107 24-25 -0.38496365321758574 1107 26-27 -0.7602475834234035 1107 28-29 -0.8498254159592165 1107 30-31 -0.4728307550315449 1107 32-33 -1.978559138411061 1107 34-35 -0.7085003298531909 1107 36-37 0.7264020796364541 1107 38-39 -0.8551345538457511 1107 40-41 -1.4459853473816935 1107 42-43 1.2634599523080219 1107 44-45 1.5036233622148032 1107 46-47 -2.51239591703672 1107 48-49 -0.696613720104331 1107 50-51 -0.745148439451846 1107 52-53 -2.1678785540586887 1107 54-55 -1.3128196638944019 1107 56-57 -1.9104058310653507 1107 58-59 -1.8067227588011363 1107 60-61 -2.8851630128625168 1107 62-63 1.3787138188721855 1107 64-65 0.5546050549648633 1107 66-67 -1.6890644388901883 1107 68-69 -2.6514375285673317 1107 70-71 -2.291297431317794 1107 72-73 -1.9750892429949758 1107 74-75 -0.5629713182256175 1107 76 -1.683006611981071 1108 1 -0.013972721171072067 1108 2 0.7311211658952814 1108 3 1.5436077809495217 1108 4 -1.1051527943341917 1108 5 -4.936720040478596 1108 6 -1.0504214434968482 1108 7 -2.769845410686216 1108 8 -1.2776356082857632 1108 9 -3.578890010556133 1108 10-11 0.5083400486297869 1108 12-13 -2.1236401341719535 1108 14-15 -4.048167130155882 1108 16-17 -1.2709373349162725 1108 18-19 -2.0849848965375664 1108 20-21 0.3547909854525422 1108 22-23 -1.4397690905246492 1108 24-25 -0.45181347006740324 1108 26-27 -0.7227017958776152 1108 28-29 -0.6712539873877859 1108 30-31 0.03266375046296233 1108 32-33 -1.4584126182645427 1108 34-35 -2.6443977657506252 1108 36-37 -0.2754294221950495 1108 38-39 -0.6655741142853131 1108 40-41 0.4129890115926642 1108 42-43 1.3202365090845767 1108 44-45 -0.1804425718511311 1108 46-47 2.4647103100695062 1108 48-49 1.7209686974780851 1108 50-51 3.0167563224529133 1108 52-53 2.1160042298240924 1108 54-55 -0.20201380308854056 1108 56-57 0.7425245718650508 1108 58-59 1.1044494023710207 1108 60-61 1.1001849724854686 1108 62-63 0.288054478212846 1108 64-65 0.13244388280368824 1108 66-67 1.6900564402306877 1108 68-69 3.005155878026077 1108 70-71 -1.0440446840650495 1108 72-73 -1.4283859462916801 1108 74-75 0.4745744693201708 1108 76 2.377432948458491 1109 1 -1.6211155783139297 1109 2 -0.9534026436285288 1109 3 -2.129011266669526 1109 4 -1.349200413381812 1109 5 0.6763751976166432 1109 6 2.0269595088841044 1109 7 -2.537702553543358 1109 8 1.2045072488570945 1109 9 0.9330147513486295 1109 10-11 2.3773876676774073 1109 12-13 2.900169389637572 1109 14-15 1.2137376317488773 1109 16-17 0.9373959984170597 1109 18-19 1.1144198653671928 1109 20-21 -2.7969947288331696 1109 22-23 -2.18084051909608 1109 24-25 -0.2375277557816915 1109 26-27 -2.213773224449046 1109 28-29 -0.32899208262588076 1109 30-31 -2.9792410114418004 1109 32-33 0.5951588103068879 1109 34-35 1.4181022342493783 1109 36-37 -0.924238946004575 1109 38-39 -2.15962173333293 1109 40-41 -0.6286776550740001 1109 42-43 -0.8375015861535182 1109 44-45 -0.15663304804160916 1109 46-47 1.3129245957837945 1109 48-49 0.50370679271618 1109 50-51 -1.6618151061185102 1109 52-53 -2.735186246366382 1109 54-55 0.40512905405432065 1109 56-57 -1.227713523373044 1109 58-59 1.0330208309424513 1109 60-61 0.5972087820092788 1109 62-63 -2.5363502836919167 1109 64-65 -0.6264846886248847 1109 66-67 0.014461202135453988 1109 68-69 -0.4591298362596383 1109 70-71 -0.37142563644599846 1109 72-73 -1.1129097558154903 1109 74-75 -1.1206636259179241 1109 76 0.24648056750610792 1110 1 -1.325661032859383 1110 2 -0.2299177951436775 1110 3 0.9505341878759275 1110 4 -0.5878367770181754 1110 5 0.4983448945863387 1110 6 -0.21925261232801674 1110 7 0.420630779789974 1110 8 -1.5417048723550266 1110 9 -0.8927428244089484 1110 10-11 0.8092058494955872 1110 12-13 -0.6074063679381858 1110 14-15 -1.634747216735967 1110 16-17 -1.6345736985526358 1110 18-19 -0.9670195285721981 1110 20-21 1.4889901196516746 1110 22-23 0.8589322081766468 1110 24-25 2.073078304824371 1110 26-27 -1.3406671638429835 1110 28-29 2.263810947677147 1110 30-31 -0.6459076781084683 1110 32-33 -0.2495381593900845 1110 34-35 -1.3413674627203207 1110 36-37 1.4659125691469406 1110 38-39 0.844166145454949 1110 40-41 1.15541325401691 1110 42-43 -0.7295470406989715 1110 44-45 1.5857911943826366 1110 46-47 3.17466702002622 1110 48-49 -0.4413689648595742 1110 50-51 1.254851560548154 1110 52-53 -1.7465498827300188 1110 54-55 0.2536139025391648 1110 56-57 0.8518319311724092 1110 58-59 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-0.9998145177202531 2116 74-75 1.6650506597963606 2116 76 1.877432948458491 2117 1 1.0519613447629936 2117 2 3.049802484576599 2117 3 2.572911810253551 2117 4 1.1988765096951113 2117 5 0.17317006941151547 2117 6 -1.3800917731671802 2117 7 -1.348599989440796 2117 8 0.5282251975750434 2117 9 1.439425007758885 2117 10-11 -1.449535409245673 2117 12-13 -0.30495881549063597 2117 14-15 -0.4913905733793271 2117 16-17 -0.6940142579931958 2117 18-19 -2.835900647453318 2117 20-21 1.4994796301411881 2117 22-23 -0.2144943652499265 2117 24-25 2.0188825006285676 2117 26-27 -3.04870912188494 2117 28-29 1.130943814810017 2117 30-31 1.9887077065069185 2117 32-33 -0.7093283691802945 2117 34-35 1.9838073624545025 2117 36-37 -1.1197517665173926 2117 38-39 -0.1051345538457511 2117 40-41 1.28478388338754 2117 42-43 -0.15961697076890502 2117 44-45 0.9843925929840331 2117 46-47 -1.5700882247290266 2117 48-49 -1.8119983354889442 2117 50-51 -2.9374561317595393 2117 52-53 -3.5140324002125354 2117 54-55 -0.44743504850978155 2117 56-57 1.4549787843192625 2117 58-59 -1.7105689126472932 2117 60-61 0.42252929482978985 2117 62-63 0.7056368957952621 2117 64-65 -2.849241098881297 2117 66-67 -1.4582952081209584 2117 68-69 3.560100932971128 2117 70-71 3.8625487225283592 2117 72-73 -1.9558584737642093 2117 74-75 3.917797912543616 2117 76 0.5862241572496991 2118 1 3.6622177550194053 2118 2 -1.278402643628528 2118 3 0.5626553999971406 2118 4 0.8091329199515229 2118 5 1.1013751976166404 2118 6 -0.5647071577825642 2118 7 3.6872974464566397 2118 8 2.7128405821904273 2118 9 -0.9503185819847033 2118 10-11 -2.572612332322592 2118 12-13 0.8001693896375706 2118 14-15 -1.7862623682511227 2118 16-17 0.4623959984170618 2118 18-19 -0.7897468012994722 2118 20-21 3.0738386045001604 2118 22-23 1.4316594809039174 2118 24-25 -0.07086108911502009 2118 26-27 2.4487267755509556 2118 28-29 -1.9664920826258836 2118 30-31 3.3707589885581974 2118 32-33 0.6368254769735522 2118 34-35 -0.7110644324172917 2118 36-37 1.8674277206620964 2118 38-39 1.2487116000004015 2118 40-41 -0.3203443217406665 2118 42-43 0.5249984138464825 2118 44-45 -1.3232997147082735 2118 46-47 0.3837579291171238 2118 48-49 0.9162067927161814 2118 50-51 -1.3451484394518474 2118 52-53 3.4064804203002836 2118 54-55 2.2217957207209835 2118 56-57 1.3806198099602902 2118 58-59 4.199687497609116 2118 60-61 2.4430421153426103 2118 62-63 2.692816382974751 2118 64-65 0.32768197804178456 2118 66-67 -1.9813721311978831 2118 68-69 -0.7091298362596383 2118 70-71 1.1369076968873344 2118 72-73 2.1954235775178432 2118 74-75 -0.6206636259179241 2118 76 -3.6035194324938917 2119 1 -0.05293376013211315 2119 2 -1.0480996133254976 2119 3 -4.594920357578616 2119 4 3.275799586618188 2119 5 -0.36529146905002463 2119 6 -0.9465253396007469 2119 7 3.6933580525172474 2119 8 1.4582951276449734 2119 9 -1.3927428244089484 2119 10-11 0.03647857676831734 2119 12-13 0.9607754502436308 2119 14-15 -0.18020176219051365 2119 16-17 -0.08911915309808904 2119 18-19 -1.5806558922085614 2119 20-21 3.716262846924405 2119 22-23 2.4952958445402835 2119 24-25 1.5958055775516442 2119 26-27 1.68206010888429 2119 28-29 2.62744731131351 2119 30-31 2.1040923218915317 2119 32-33 1.9095527497008256 2119 34-35 0.34045071909786273 2119 36-37 -1.193178339943966 2119 38-39 0.8214388727276756 2119 40-41 0.6099587085623668 2119 42-43 0.8159075047555717 2119 44-45 1.085791194382633 2119 46-47 0.1746670200262166 2119 48-49 1.1495401260495157 2119 50-51 0.754851560548154 2119 52-53 -0.04200442818456196 2119 54-55 0.41270481163007844 2119 56-57 0.556377385717866 2119 58-59 2.9118087097303302 2119 60-61 -1.8327154604149634 2119 62-63 -1.3223351321767645 2119 64-65 0.22768197804178314 2119 66-67 3.4368096869839384 2119 68-69 0.24541561828581493 2119 70-71 -0.7843044243247874 2119 72-73 3.6105750926693574 2119 74-75 -2.166118171372471 2119 76 1.8449654159909592 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 2.0 12 14.0 13 114.0 14 338.0 15 559.0 16 678.0 17 844.0 18 919.0 19 758.0 20 724.0 21 574.0 22 363.0 23 292.0 24 175.0 25 150.0 26 82.0 27 55.0 28 52.0 29 38.0 30 36.0 31 33.0 32 26.0 33 39.0 34 36.0 35 70.0 36 97.0 37 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.611142374788971 29.009566685424875 7.597073719752391 55.78221722003377 2 2.9234858022100996 44.09008252902504 25.02447894810463 27.961952720660232 3 3.860679815358791 29.402713666247028 19.932857742341586 46.80374877605259 4 3.678836200867254 28.899146733808923 31.2211498111624 36.20086725416142 5 3.594908378794237 32.78780248985872 33.68303259197091 29.934256539376136 6 5.371380612673101 30.18604000559519 30.01818436144915 34.42439502028255 7 14.561477129668484 36.312771016925446 15.009092180724576 34.11665967268149 8 5.637152049237656 36.312771016925446 25.430130088124216 32.61994684571269 9 11.246328157784305 28.42355574206183 15.946286193873268 44.383829906280596 10-11 7.679395719681074 29.605539236256817 27.0877045740663 35.62736046999581 12-13 6.616309973422857 27.17862638131207 25.02447894810463 41.18058469716044 14-15 11.533081549867115 31.305077633235417 19.100573506784166 38.0612673101133 16-17 16.32396139320185 30.18604000559519 19.149531402993425 34.34046719820954 18-19 12.365365785424535 28.213736186879284 17.792698279479644 41.62819974821653 20-21 10.351098055672121 28.815218911735908 18.967687788501888 41.86599524409008 22-23 17.51993285774234 29.17890614071898 16.309973422856345 36.991187578682336 24-25 13.470415442719261 34.94894390823892 14.708350818296264 36.87228983074556 26-27 10.407049937054133 35.80920408448734 19.925863757168834 33.85788222128969 28-29 13.813120716184082 30.458805427332493 24.597845852566795 31.13022800391663 30-31 13.491397398237515 29.500629458665546 25.577003776751994 31.43096936634494 32-33 10.253182263253603 35.29164918170373 23.038187159043222 31.41698139599944 34-35 9.71464540495174 38.05427332494055 19.492236676458248 32.73884459364946 36-37 11.337249965030075 31.64778290670024 22.135963071758287 34.8790040565114 38-39 12.512239474052315 25.101412785004896 27.738145195132187 34.648202545810605 40-41 9.476849909078194 27.836060987550702 25.47908798433347 37.208001119037625 42-43 5.700097915792419 24.69576164498531 28.33263393481606 41.271506504406204 44-45 7.16883480207022 25.26227444397818 31.74569869911876 35.823192054832845 46-47 6.952021261714925 30.703594908378797 28.451531682752833 33.89285214715345 48-49 7.252762624143237 33.86487620646244 21.597426213456426 37.28493495593789 50-51 6.952021261714925 33.85088823611694 18.862778010910617 40.33431249125752 52-53 5.623164078892152 33.27738145195132 20.842075814799273 40.257378654357254 54-55 6.420478388585817 30.84347461183382 20.324520912015668 42.411526087564695 56-57 10.525947684990907 31.605818995663732 19.20548328437544 38.66275003496993 58-59 9.833543152888517 31.242131766680654 17.89760805707092 41.02671702335991 60-61 7.147852846551965 28.633375297244367 19.834941949923067 44.383829906280596 62-63 10.022380752552804 29.087984333473216 18.226325360190238 42.66330955378375 64-65 10.386067981535879 30.24898587214995 15.3657854245349 43.99916072177927 66-67 7.959155126591131 36.270807105888935 12.847950762344384 42.92208700517555 68-69 10.071338648762064 36.599524409008254 12.512239474052315 40.816897468177366 70-71 11.274304098475312 35.69030633655056 12.253462022660512 40.78192754231361 72-73 10.00139879703455 37.41082668904742 11.798852986431669 40.78892152748636 74-75 10.379073996363127 33.52916491817037 14.806266610714786 41.285494474751715 76 7.679395719681074 28.927122674499927 12.379353755770039 51.014127850048965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 1.0 11 1.0 12 0.0 13 1.0 14 1.5 15 2.0 16 2.5 17 2.0 18 4.0 19 6.5 20 8.5 21 10.0 22 7.5 23 7.0 24 16.5 25 24.0 26 27.0 27 34.0 28 37.5 29 37.0 30 50.0 31 58.0 32 70.5 33 88.0 34 103.5 35 141.0 36 152.5 37 142.0 38 174.5 39 241.5 40 286.5 41 319.5 42 342.0 43 359.0 44 375.5 45 400.0 46 425.0 47 436.5 48 455.5 49 437.5 50 412.0 51 426.0 52 414.0 53 371.5 54 355.0 55 338.5 56 289.5 57 235.5 58 214.0 59 199.0 60 161.0 61 120.5 62 103.0 63 85.0 64 58.5 65 52.5 66 43.5 67 32.0 68 29.5 69 21.0 70 14.0 71 13.0 72 13.0 73 9.0 74 5.5 75 6.0 76 6.0 77 6.0 78 5.5 79 5.0 80 4.5 81 3.0 82 1.5 83 1.0 84 2.0 85 2.5 86 2.5 87 3.0 88 2.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5735067841656176 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7149.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.25150370681214 #Duplication Level Percentage of deduplicated Percentage of total 1 98.90375854214123 97.17442999020842 2 0.683371298405467 1.3428451531682752 3 0.24202733485193623 0.7133864876206463 4 0.12813211845102507 0.5035669324381032 5 0.014236902050113895 0.06993985172751434 6 0.0 0.0 7 0.02847380410022779 0.19583158483704013 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05595188138201147 0.0 2 0.0 0.0 0.0 0.05595188138201147 0.0 3 0.0 0.0 0.0 0.06993985172751434 0.0 4 0.0 0.0 0.0 0.0839278220730172 0.0 5 0.0 0.0 0.0 0.0839278220730172 0.0 6 0.0 0.0 0.0 0.0839278220730172 0.0 7 0.0 0.0 0.0 0.0839278220730172 0.0 8 0.0 0.0 0.0 0.0839278220730172 0.0 9 0.0 0.0 0.0 0.0839278220730172 0.0 10 0.0 0.0 0.0 0.0839278220730172 0.0 11 0.0 0.0 0.0 0.09791579241852007 0.0 12 0.0 0.0 0.0 0.15386767380053154 0.0 13 0.0 0.0 0.0 0.15386767380053154 0.0 14 0.0 0.0 0.0 0.18184361449153727 0.0 15 0.0 0.0 0.0 0.2238075255280459 0.0 16 0.0 0.0 0.0 0.2517834662190516 0.0 17 0.0 0.0 0.0 0.2937473772555602 0.0 18 0.0 0.0 0.0 0.4336270807105889 0.0 19 0.0 0.0 0.0 0.5595188138201147 0.0 20 0.0 0.0 0.0 0.6154706952021262 0.0 21 0.0 0.0 0.0 0.6714225765841376 0.0 22 0.0 0.0 0.0 0.7553503986571548 0.0 23 0.0 0.0 0.0 0.7693383690026577 0.0 24 0.0 0.0 0.0 0.7693383690026577 0.0 25 0.0 0.0 0.0 0.7693383690026577 0.0 26 0.0 0.0 0.0 0.7693383690026577 0.0 27 0.0 0.0 0.0 0.8113022800391663 0.0 28 0.0 0.0 0.0 0.8252902503846692 0.0 29 0.0 0.0 0.0 0.839278220730172 0.0 30 0.0 0.0 0.0 0.839278220730172 0.0 31 0.0 0.0 0.0 0.8672541614211778 0.0 32 0.0 0.0 0.0 0.9651699538396978 0.0 33 0.0 0.0 0.0 0.9931458945307036 0.0 34 0.0 0.0 0.0 1.063085746258218 0.0 35 0.0 0.0 0.0 1.0770737166037208 0.0 36 0.0 0.0 0.0 1.2029654497132467 0.0 37 0.0 0.0 0.0 1.3008812421317666 0.0 38 0.0 0.0 0.0 1.5107007973143096 0.0 39 0.0 0.0 0.0 1.7904602042243671 0.0 40 0.0 0.0 0.0 1.9583158483704015 0.0 41 0.0 0.0 0.0 2.126171492516436 0.0 42 0.0 0.0 0.0 2.3779549587354873 0.0 43 0.0 0.0 0.0 2.4758707511540075 0.0 44 0.0 0.0 0.0 2.6157504546090364 0.0 45 0.0 0.0 0.0 2.741642187718562 0.0 46 0.0 0.0 0.0 2.9094978318645963 0.0 47 0.0 0.0 0.0 3.0493775353196253 0.0 48 0.0 0.0 0.0 3.133305357392642 0.0 49 0.0 0.0 0.0 3.3291369422296824 0.0 50 0.0 0.0 0.0 3.4130647643026997 0.0 51 0.0 0.0 0.0 3.4690166456847114 0.0 52 0.0 0.0 0.0 3.594908378794237 0.0 53 0.0 0.0 0.0 3.692824171212757 0.0 54 0.0 0.0 0.0 3.720800111903763 0.0 55 0.0 0.0 0.0 3.874667785704294 0.0 56 0.0 0.0 0.0 4.084487340886837 0.0 57 0.0 0.0 0.0 4.154427192614351 0.0 58 0.0 0.0 0.0 4.29430689606938 0.0 59 0.0 0.0 0.0 4.378234718142398 0.0 60 0.0 0.0 0.0 4.420198629178906 0.0 61 0.0 0.0 0.0 4.546090362288432 0.0 62 0.0 0.0 0.0 4.616030214015947 0.0 63 0.0 0.0 0.0 4.671982095397958 0.0 64 0.0 0.0 0.0 4.685970065743461 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTCAA 20 0.006674931 52.494717 58 CCCACCC 95 0.006548923 18.419199 62 >>END_MODULE