FastQCFastQC Report
Wed 25 May 2016
SRR1780716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7149
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT255135.683312351377815TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT6699.357952161141418TruSeq Adapter, Index 5 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG3574.993705413344523TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2173.035389564974122RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT1882.6297384249545392No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC1251.7484962931878585RNA PCR Primer, Index 5 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT871.2169534200587495RNA PCR Primer, Index 5 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT871.2169534200587495No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT831.161001538676738No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC530.741362428311652TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGCGACACAAAGTGATCTCGTATGCCGT520.7273744579661491Illumina PCR Primer Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCG400.5595188138201147No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC370.5175549027836062RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGT360.5035669324381032No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGTCTT340.4755909917470975Illumina PCR Primer Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCG340.4755909917470975No Hit
CTTATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGT300.41963911036508605No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAGTGATCTCGTATGCCGT280.39166316967408027Illumina PCR Primer Index 5 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCC260.36368722898307454No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC260.36368722898307454TruSeq Adapter, Index 5 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG240.3357112882920688No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT240.3357112882920688No Hit
CTTATACACATCTCCGAGCCCACGAGCCACAAAGTGATCTCGTATGCCGT240.3357112882920688TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGT210.2937473772555602Illumina PCR Primer Index 5 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGTCTT200.27975940691005735Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAAAAGTGATCTCGTATGCCGT200.27975940691005735No Hit
CTTATACACATCTCCGAGCACACGAGACACAAAGTGATCTCGTATGCCGT190.2657714365645545Illumina PCR Primer Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCAAGAGACACAAAGTGATCTCGTATGCCGT190.2657714365645545Illumina PCR Primer Index 5 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCT180.2517834662190516RNA PCR Primer, Index 5 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCC160.22380752552804586No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAAATCGTATGCCGT150.20981955518254303No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT150.20981955518254303No Hit
CTTATACACATCTCCGAGCCCACGTGACACAAAGTGATCTCGTATGCCGT150.20981955518254303Illumina PCR Primer Index 5 (95% over 23bp)
CTTATACACATATCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT130.18184361449153727RNA PCR Primer, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT130.18184361449153727No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC120.1678556441460344No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCC120.1678556441460344No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATTTCGTATGCCGT120.1678556441460344No Hit
ATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGTCTT120.1678556441460344Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT110.15386767380053154No Hit
CTTAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC110.15386767380053154TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTCTGCCGT110.15386767380053154No Hit
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT110.15386767380053154TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGCTCTCGTATGCCGT110.15386767380053154No Hit
ATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTGCTT100.13987970345502868TruSeq Adapter, Index 5 (96% over 32bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGTCTT100.13987970345502868Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCCCGTATGCCGT100.13987970345502868No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG100.13987970345502868TruSeq Adapter, Index 5 (95% over 22bp)
CTTATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT100.13987970345502868No Hit
CTTATACACATCTACGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT90.1258917331095258TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGTCTT90.1258917331095258Illumina PCR Primer Index 5 (95% over 22bp)
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA90.1258917331095258No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACATCGTATGCCGT90.1258917331095258No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGTC80.11190376276402293No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGCGATCTCGTATGCCGT80.11190376276402293No Hit
CTTATCACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC80.11190376276402293TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCCT80.11190376276402293TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACACGTATGCCGT80.11190376276402293No Hit
CTTATACAAATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT80.11190376276402293RNA PCR Primer, Index 5 (95% over 23bp)
CATTAGAGTCTCTTGGAACAAATTGACAAACTGGTGTGTTTTCCTTCATT80.11190376276402293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG150.002134695970.0000120
CACACGA150.002134695970.0000119
CTTTACA150.002134695970.000011
AGCCACA150.002134695970.0000125
AAATAAA150.002134695970.0000170
TTACACA150.002134695970.000013
GAGCCAC207.399345E-570.0000124
CACGAGC150.002134695970.0000121
GAGCACA150.002134695970.0000116
ACGAGCC150.002134695970.0000122
CGAGCCA207.399345E-570.0000123
CGAGCAC150.002134695970.0000115
CCGAGCA150.002134695970.0000114
GCCACAA150.002134695970.0000126
AGCACAC150.002134695970.0000117
TTTACAC150.002134695970.000012
GCACACG150.002134695970.0000118
ACCAAGT200.006672273452.50000429
CACCAAG200.006672273452.50000428
AAAATAA200.006672273452.50000469
Produced by FastQC (version 0.11.5)