##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780716_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7149 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76108546649881 34.0 34.0 34.0 31.0 34.0 2 33.03636872289831 34.0 34.0 34.0 32.0 34.0 3 33.21695342005875 34.0 34.0 34.0 32.0 34.0 4 33.467198209539795 34.0 34.0 34.0 34.0 34.0 5 33.37865435725276 34.0 34.0 34.0 34.0 34.0 6 37.28493495593789 38.0 37.0 38.0 36.0 38.0 7 36.71828227724157 38.0 37.0 38.0 34.0 38.0 8 36.787662610155266 38.0 37.0 38.0 34.0 38.0 9 36.5925304238355 38.0 37.0 38.0 34.0 38.0 10-11 36.24101272905301 38.0 37.0 38.0 34.0 38.0 12-13 36.08595607777312 38.0 37.0 38.0 33.0 38.0 14-15 36.10938592810183 38.0 36.5 38.0 33.0 38.0 16-17 35.886417680794516 38.0 36.0 38.0 31.0 38.0 18-19 35.73947405231501 38.0 35.5 38.0 31.0 38.0 20-21 35.57791299482445 38.0 36.0 38.0 31.0 38.0 22-23 35.91558259896489 38.0 36.5 38.0 32.5 38.0 24-25 34.27563295565813 37.0 33.5 38.0 26.5 38.0 26-27 34.41747097496153 37.0 34.0 38.0 25.0 38.0 28-29 34.95153168275283 37.0 35.0 38.0 29.0 38.0 30-31 34.393971184781094 37.0 34.0 38.0 26.5 38.0 32-33 34.628339627919985 37.0 34.5 38.0 27.0 38.0 34-35 33.78416561756889 37.0 34.0 38.0 23.5 38.0 36-37 33.92971044901385 37.0 34.0 38.0 24.0 38.0 38-39 29.01496712826969 34.0 24.0 37.0 11.0 38.0 40-41 32.02797594069101 35.0 30.5 37.0 17.5 38.0 42-43 34.620926003636875 37.0 34.5 38.0 28.5 38.0 44-45 35.10889634913974 37.0 34.5 38.0 29.0 38.0 46-47 35.31333053573927 37.5 36.0 38.0 31.0 38.0 48-49 34.70065743460624 37.5 35.0 38.0 28.0 38.0 50-51 34.47489159322982 37.0 34.0 38.0 26.0 38.0 52-53 34.093439641907956 37.0 34.0 38.0 24.5 38.0 54-55 34.691775073436844 37.0 34.5 38.0 27.0 38.0 56-57 34.76010630857462 37.0 34.5 38.0 27.0 38.0 58-59 34.05777031752693 37.0 34.0 38.0 24.0 38.0 60-61 33.954818855784026 37.0 34.0 38.0 24.5 38.0 62-63 34.79577563295565 37.0 35.0 38.0 27.0 38.0 64-65 34.94390823891453 37.0 35.5 38.0 29.0 38.0 66-67 32.88459924464961 37.0 31.0 38.0 21.5 38.0 68-69 29.912994824450973 34.0 24.0 37.5 11.0 38.0 70-71 26.14890194432788 28.5 16.5 35.5 10.5 37.5 72-73 21.052734648202545 22.0 11.0 28.0 10.5 35.0 74-75 20.226954818855784 21.0 11.0 26.0 11.0 34.0 76 20.457686389704854 21.0 11.0 27.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2534446754248734 1101 2 0.8256141315368097 1101 3 0.8379511090994711 1101 4 0.5465765641081362 1101 5 0.6933220826120419 1101 6 -1.100024899375967 1101 7 0.7408839750087495 1101 8 1.0888917965826366 1101 9 1.5208395753939215 1101 10-11 1.7185715303222793 1101 12-13 1.6127024851630765 1101 14-15 1.647312182051472 1101 16-17 1.3225357894644603 1101 18-19 0.4896589579581132 1101 20-21 0.5427312143548733 1101 22-23 1.6997049224854948 1101 24-25 -2.1212328073497915 1101 26-27 -0.3676715856405721 1101 28-29 1.693248081106148 1101 30-31 1.3600462656312402 1101 32-33 0.0053317332460309785 1101 34-35 -0.8623160370421985 1101 36-37 -0.23234867204925536 1101 38-39 0.7906233348853 1101 40-41 2.9757192642600785 1101 42-43 0.4958051314802461 1101 44-45 2.2631970689518184 1101 46-47 2.2277731967849306 1101 48-49 -2.202593798665987 1101 50-51 -0.15588967610884197 1101 52-53 -0.6808212107040816 1101 54-55 0.9298232403606548 1101 56-57 -0.014878464193273544 1101 58-59 -0.7902540411266159 1101 60-61 -2.8976179587119475 1101 62-63 -0.14885537539471017 1101 64-65 -1.1211710679919946 1101 66-67 0.3051860800674291 1101 68-69 1.5924026012687023 1101 70-71 -1.7693503254571326 1101 72-73 0.9403332439766103 1101 74-75 -1.3129160831112152 1101 76 -3.133091690663175 1102 1 0.4727429210389076 1102 2 0.8256141315368097 1102 3 0.8379511090994711 1102 4 0.7132432307748005 1102 5 0.6933220826120419 1102 6 0.683600831618179 1102 7 -1.022273919728093 1102 8 0.9426929661732757 1102 9 0.37171676837637335 1102 10-11 1.3676943373398274 1102 12-13 1.7442814325315013 1102 14-15 0.45871569082339647 1102 16-17 0.5564539181194235 1102 18-19 -0.04689074964421991 1102 20-21 1.1655382318987293 1102 22-23 0.5315762675147369 1102 24-25 -6.822987193314706 1102 26-27 -0.8413557961668872 1102 28-29 0.7985112390008879 1102 30-31 2.5267129322979116 1102 32-33 2.527261557807435 1102 34-35 2.158151799215112 1102 36-37 -2.4779627071369816 1102 38-39 0.10056485535313087 1102 40-41 3.152619849055398 1102 42-43 1.3086706285562641 1102 44-45 2.7134894666126357 1102 46-47 1.887129921931134 1102 48-49 -3.690897892233238 1102 50-51 -0.5608604363427574 1102 52-53 -0.08725395924209067 1102 54-55 0.5789460473781958 1102 56-57 0.9485718282043933 1102 58-59 0.8588687658909251 1102 60-61 -7.656389888536509 1102 62-63 -4.189791047909331 1102 64-65 0.7545599261600486 1102 66-67 3.485010641470943 1102 68-69 -0.8008722525324643 1102 70-71 -2.3833854131764305 1102 72-73 -1.4134679256140323 1102 74-75 1.1914698818010692 1102 76 -4.2208109889087915 1103 1 0.8784446754248734 1103 2 0.6589474648701454 1103 3 0.6712844424328068 1103 4 0.7132432307748005 1103 5 0.19332208261204187 1103 6 0.4555306561795831 1103 7 0.9908839750087495 1103 8 1.186114018804858 1103 9 1.3125062420605857 1103 10-11 1.551904863655615 1103 12-13 0.9252024851630765 1103 14-15 0.6889788487181363 1103 16-17 0.3503135672422317 1103 18-19 1.316047846846999 1103 20-21 -0.6239354523117981 1103 22-23 0.3594271447077233 1103 24-25 -4.662899474016463 1103 26-27 -0.22183825230724352 1103 28-29 0.3182480811061481 1103 30-31 2.130879598964583 1103 32-33 3.005331733246024 1103 34-35 2.3807395185133515 1103 36-37 -0.08651533871592676 1103 38-39 -2.0496544428924786 1103 40-41 -0.43400295796214294 1103 42-43 0.5027495759246889 1103 44-45 2.3187526245073755 1103 46-47 2.0472176412293805 1103 48-49 -0.7303715764437655 1103 50-51 0.42049921278004376 1103 52-53 1.5552899004070255 1103 54-55 0.5548232403606548 1103 56-57 1.707343758028955 1103 58-59 2.022245958873384 1103 60-61 -5.66845129204528 1103 62-63 -1.093299819839153 1103 64-65 0.8927178208968911 1103 66-67 0.3885194134007719 1103 68-69 1.3910137123798165 1103 70-71 3.043149674542864 1103 72-73 -4.136055644912279 1103 74-75 0.7079172502221205 1103 76 -2.4664250239965106 1104 1 0.34011134209153937 1104 2 -0.33438586846318685 1104 3 0.6379511090994683 1104 4 0.15324323077479818 1104 5 0.1333220826120396 1104 6 0.2688639895129157 1104 7 0.5608839750087498 1104 8 1.0311140188048569 1104 9 1.0875062420605843 1104 10-11 -1.428095136344389 1104 12-13 0.6127024851630765 1104 14-15 0.1439788487181346 1104 16-17 -0.6930197660910977 1104 18-19 0.6152145135136706 1104 20-21 -1.343935452311797 1104 22-23 0.4852604780410559 1104 24-25 1.34543385931687 1104 26-27 2.392328414359433 1104 28-29 2.3332480811061487 1104 30-31 1.5267129322979116 1104 32-33 1.4619983999126944 1104 34-35 0.0865728518466895 1104 36-37 2.3809846612840744 1104 38-39 -0.14048777622581454 1104 40-41 1.2368303753711984 1104 42-43 -0.7197504240753076 1104 44-45 1.3987526245073738 1104 46-47 0.6555509745627077 1104 48-49 0.9396284235562362 1104 50-51 0.8096658794467118 1104 52-53 -0.7430434329263065 1104 54-55 0.12315657369398281 1104 56-57 1.0106770913622825 1104 58-59 -0.4769207077932833 1104 60-61 0.4257153746213831 1104 62-63 -0.3266331531724873 1104 64-65 1.4577178208968888 1104 66-67 0.2218527467341005 1104 68-69 3.0401803790464825 1104 70-71 3.9039830078761995 1104 72-73 -2.081888978245612 1104 74-75 1.870417250222122 1104 76 1.160241642670158 1105 1 1.1508805728607712 1105 2 0.7871525930752696 1105 3 0.8379511090994711 1105 4 0.5978586153901873 1105 5 0.46255285184280837 1105 6 -0.057289856640927894 1105 7 0.9331916673164429 1105 8 -0.9581167504259085 1105 9 1.0721216266759725 1105 10-11 0.013443325194074873 1105 12-13 -0.17575905329846364 1105 14-15 0.660132694871983 1105 16-17 0.9208263877550564 1105 18-19 0.29521451351367034 1105 20-21 0.5106799323035887 1105 22-23 0.9283374011179717 1105 24-25 1.372356936239953 1105 26-27 1.247713029744041 1105 28-29 0.8663250041830679 1105 30-31 1.3920975476825248 1105 32-33 0.21045993837423538 1105 34-35 -2.5888117635379295 1105 36-37 0.13944619974560624 1105 38-39 2.371819916081879 1105 40-41 0.7791380676788862 1105 42-43 1.3825572682323823 1105 44-45 1.7225987783535288 1105 46-47 1.3324740514857893 1105 48-49 -1.4803715764437655 1105 50-51 1.5535120332928685 1105 52-53 0.23157195168908373 1105 54-55 0.6862334967709032 1105 56-57 0.9445232452084369 1105 58-59 -1.514613015485594 1105 60-61 0.3780230669290816 1105 62-63 0.015674539135204668 1105 64-65 1.4984870516661175 1105 66-67 1.8949296698110203 1105 68-69 -0.9759734671073659 1105 70-71 1.8139830078761996 1105 72-73 -2.281888978245611 1105 74-75 0.19349417329904384 1105 76 -2.2612968188683027 1106 1 -0.9132219912417945 1106 2 0.6351379410606199 1106 3 0.6950939662423252 1106 4 0.7132432307748005 1106 5 0.6933220826120419 1106 6 0.3126735133224443 1106 7 -1.235306501181725 1106 8 0.025399733090573307 1106 9 -0.31249375793941425 1106 10-11 0.6471429588937099 1106 12-13 1.6603215327821275 1106 14-15 0.4449312296705159 1106 16-17 0.45745642438509293 1106 18-19 0.628547846847006 1106 20-21 1.685588357212012 1106 22-23 1.4338319066124825 1106 24-25 -0.6688518549688425 1106 26-27 1.7037569857879973 1106 28-29 1.8122957001537685 1106 30-31 0.07433197991696261 1106 32-33 0.4696174475317463 1106 34-35 2.812287137560972 1106 36-37 2.791460851760263 1106 38-39 -0.46334491908296016 1106 40-41 2.503497042037864 1106 42-43 2.3688210044961195 1106 44-45 2.5568478626026163 1106 46-47 2.0650747840865193 1106 48-49 2.5672474711752855 1106 50-51 1.1258563556371897 1106 52-53 0.5255279956451275 1106 54-55 0.5250613355987426 1106 56-57 1.457343758028955 1106 58-59 2.0430792922067127 1106 60-61 0.8523820412880596 1106 62-63 0.31146208492275207 1106 64-65 -2.982282179103109 1106 66-67 -0.9210043961230419 1106 68-69 2.429704188570291 1106 70-71 1.778268722161915 1106 72-73 2.122872926516294 1106 74-75 1.818036297841168 1106 76 3.3430987855272996 1107 1 1.3431882651684575 1107 2 -0.6743858684631903 1107 3 0.299489570637931 1107 4 -0.47906446153289295 1107 5 -0.7297548404648779 1107 6 0.3273255279744589 1107 7 1.010114744239516 1107 8 0.1957294034202448 1107 9 0.41827547282981925 1107 10-11 1.3980587098094617 1107 12-13 -0.6949898225292301 1107 14-15 0.160132694871983 1107 16-17 -0.34840438147571007 1107 18-19 0.7375222058213637 1107 20-21 0.26067993230358866 1107 22-23 -0.5139702911897217 1107 24-25 -0.839181525298514 1107 26-27 -1.5984408164097985 1107 28-29 0.9432480811061481 1107 30-31 -0.030979375394394992 1107 32-33 1.806613784528082 1107 34-35 -0.39650407123023257 1107 36-37 1.3125231228225331 1107 38-39 1.4295122237741857 1107 40-41 -0.1824003938595773 1107 42-43 0.8633264990016087 1107 44-45 1.2802910860458354 1107 46-47 1.140166359178096 1107 48-49 0.8657822697100812 1107 50-51 1.7073581871390218 1107 52-53 1.4431104132275436 1107 54-55 0.57084888138629 1107 56-57 1.2137540144392034 1107 58-59 0.7930792922067127 1107 60-61 1.3010999900060014 1107 62-63 0.669520692981358 1107 64-65 -0.2515129483338825 1107 66-67 -0.9512241763428229 1107 68-69 -0.8605888517227491 1107 70-71 -3.6475554536622603 1107 72-73 0.7181110217543889 1107 74-75 -2.306505826700956 1107 76 -2.299758357329843 1108 1 -0.7703648483846521 1108 2 0.30180460772728424 1108 3 0.21890349005185072 1108 4 0.6180051355367056 1108 5 -0.020963631673673433 1108 6 0.4079116085605392 1108 7 0.2885030226278005 1108 8 -0.4507907430999012 1108 9 0.5922681468224908 1108 10-11 1.1233334350841844 1108 12-13 1.8031786756392663 1108 14-15 1.1830264677657567 1108 16-17 0.9098373767660419 1108 18-19 0.7952145135136703 1108 20-21 1.3046359762596325 1108 22-23 0.410022382802957 1108 24-25 2.5216243355073473 1108 26-27 -0.4867192046881925 1108 28-29 0.3361052239632869 1108 30-31 1.3600462656312473 1108 32-33 0.8981888761031698 1108 34-35 1.4313347566085923 1108 36-37 1.8628894231888324 1108 38-39 3.8223693666313245 1108 40-41 3.408258946799762 1108 42-43 1.7021543378294481 1108 44-45 1.5806573864121347 1108 46-47 1.541265260277001 1108 48-49 2.3767712806990957 1108 50-51 2.387761117541956 1108 52-53 1.3112422813594122 1108 54-55 -0.07017675963934522 1108 56-57 -0.06646576578057761 1108 58-59 1.5906983398257637 1108 60-61 0.7809534698594831 1108 62-63 1.9543192277798909 1108 64-65 0.8986702018492707 1108 66-67 2.0313765562579107 1108 68-69 5.57256133142743 1108 70-71 -0.05506461117142081 1108 72-73 0.0990634027067685 1108 74-75 1.698988678793551 1108 76 -0.5616631192346055 1109 1 -1.3715553245751302 1109 2 -0.29938586846319026 1109 3 -0.20371555756719317 1109 4 0.08824323077480045 1109 5 -0.9316779173879581 1109 6 -1.8361360104870812 1109 7 0.28255064167541377 1109 8 -0.022219314528477696 1109 9 0.72917290872725 1109 10-11 0.26023819698894357 1109 12-13 0.7377024851630765 1109 14-15 1.272312182051472 1109 16-17 1.2461469005755745 1109 18-19 1.316047846846999 1109 20-21 1.5010645476882019 1109 22-23 1.255260478041052 1109 24-25 -0.49623280734979147 1109 26-27 0.5281617476927636 1109 28-29 -0.9942519188938519 1109 30-31 0.7975462656312402 1109 32-33 0.5678317332460239 1109 34-35 0.8599061851800229 1109 36-37 1.934317994617409 1109 38-39 0.40867889044085004 1109 40-41 -1.8715029579621394 1109 42-43 1.127749575924689 1109 44-45 0.6729192911740398 1109 46-47 1.0263843078960448 1109 48-49 2.061295090222899 1109 50-51 -1.2878341205532848 1109 52-53 -0.6947100995929674 1109 54-55 -0.23684342630601662 1109 56-57 0.1656770913622836 1109 58-59 -1.3319207077932873 1109 60-61 0.4982153746213882 1109 62-63 0.17753351349418267 1109 64-65 1.1010511542302268 1109 66-67 0.5135194134007648 1109 68-69 -0.9631529542868549 1109 70-71 0.29314967454286744 1109 72-73 0.6764443550877246 1109 74-75 -0.5837494164445474 1109 76 -4.383091690663175 1110 1 -0.03443411245391559 1110 2 0.5074323133549896 1110 3 -0.20750343635507562 1110 4 -0.9685749510433794 1110 5 0.5569584462484087 1110 6 0.42522762587655194 1110 7 0.8772476113723826 1110 8 0.9474776551684911 1110 9 1.3693244238787656 1110 10-11 0.16554122729198184 1110 12-13 0.521793394253983 1110 14-15 1.0185243032635896 1110 16-17 0.948798415727083 1110 18-19 -0.02296730466814978 1110 20-21 1.080610002233655 1110 22-23 -0.1538304310498546 1110 24-25 -1.068202504319487 1110 26-27 1.9050556870867013 1110 28-29 1.6705208083788747 1110 30-31 1.5721674777524584 1110 32-33 1.5129074908217888 1110 34-35 0.7993001245739606 1110 36-37 1.1009846612840732 1110 38-39 2.815875860137819 1110 40-41 -1.299533260992444 1110 42-43 0.3038859395610487 1110 44-45 0.409661715416469 1110 46-47 0.48282370183543577 1110 48-49 2.8150829690107813 1110 50-51 1.9905749703558087 1110 52-53 1.6196838398009703 1110 54-55 -0.39593433539692313 1110 56-57 1.44976800045319 1110 58-59 -0.6160116168841938 1110 60-61 1.231169920075935 1110 62-63 1.2324577559184178 1110 64-65 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-0.2461746925313264 2116 74-75 2.0204172502221205 2116 76 -3.3711869287584157 2117 1 1.3431882651684575 2117 2 0.8256141315368097 2117 3 -1.6235873524389888 2117 4 -1.2098336923021193 2117 5 -0.38360099431103833 2117 6 0.3273255279744589 2117 7 0.7793455134702896 2117 8 -0.3042705965797552 2117 9 -3.158647604093261 2117 10-11 -2.871172059421305 2117 12-13 -3.233451360990763 2117 14-15 -2.3206365358972505 2117 16-17 -3.0022505353218705 2117 18-19 -2.858631640332483 2117 20-21 0.18375685538050845 2117 22-23 0.8898758626564387 2117 24-25 -0.2622584483754338 2117 26-27 -2.713825431794419 2117 28-29 -0.5375211496630783 2117 30-31 1.9113283169132984 2117 32-33 -5.385693907779611 2117 34-35 -0.7041963789225392 2117 36-37 -1.8990153387159268 2117 38-39 -1.5127954685335077 2117 40-41 0.02913806767888616 2117 42-43 1.0171726528477691 2117 44-45 -1.7966319908772377 2117 46-47 -1.7444490254372909 2117 48-49 -2.134217730289919 2117 50-51 -0.042641812860978234 2117 52-53 -1.2491972790801498 2117 54-55 -2.006074195536783 2117 56-57 -1.2477844470992494 2117 58-59 -1.8799976308702142 2117 60-61 -2.2373615484555387 2117 62-63 -0.6958639224032552 2117 64-65 -1.2130514098723424 2117 66-67 -0.4319934071120528 2117 68-69 1.6201803790464808 2117 70-71 -2.128324684431494 2117 72-73 -0.1280428243994578 2117 74-75 -1.6911212113163394 2117 76 1.9694724119009273 2118 1 -2.1132219912417973 2118 2 -0.5743858684631888 2118 3 -1.9620488909005296 2118 4 0.3132432307748019 2118 5 0.6933220826120419 2118 6 0.7221973228462488 2118 7 1.1075506416754166 2118 8 0.7111140188048566 2118 9 -4.979160424606082 2118 10-11 -1.948095136344385 2118 12-13 -2.920630848170255 2118 14-15 0.26397884871813915 2118 16-17 -5.533019766091094 2118 18-19 -1.6381188198196668 2118 20-21 -0.9239354523117953 2118 22-23 0.805260478041049 2118 24-25 -1.7878994740164558 2118 26-27 -5.367671585640572 2118 28-29 -1.673418585560519 2118 30-31 -2.6399537343687527 2118 32-33 -2.5780016000873047 2118 34-35 -0.00676048148664421 2118 36-37 -5.632348672049261 2118 38-39 -2.253821109559148 2118 40-41 0.07016370870452704 2118 42-43 0.4069162425913575 2118 44-45 -6.114580708825958 2118 46-47 -2.3444490254372923 2118 48-49 -0.8470382431104326 2118 50-51 -3.9836674538866177 2118 52-53 -2.603043432926306 2118 54-55 -5.770176759639348 2118 56-57 -0.7426562419710478 2118 58-59 -5.356920707793286 2118 60-61 -4.847617958711943 2118 62-63 -0.6266331531724916 2118 64-65 0.45105115423022824 2118 66-67 -2.011480586599234 2118 68-69 -3.146486287620185 2118 70-71 -3.8026836587904675 2118 72-73 0.18477768842105746 2118 74-75 1.6370839168887876 2118 76 -1.133091690663175 2119 1 1.3292022511824442 2119 2 -0.44711314119046364 2119 3 -0.5256852545368957 2119 4 0.4405159580475271 2119 5 -0.30667791738795813 2119 6 -0.7565905559416279 2119 7 -0.0318432977185239 2119 8 -0.05252234483150886 2119 9 0.2329607875151325 2119 10-11 -1.3571860454352915 2119 12-13 -2.023661151200564 2119 14-15 -0.5269302421909572 2119 16-17 -1.7330197660910969 2119 18-19 -1.1138763955772362 2119 20-21 -2.5330263614027047 2119 22-23 -1.6311031583225883 2119 24-25 0.7727065865895995 2119 26-27 0.7232375052685214 2119 28-29 -1.8522064643483986 2119 30-31 1.299440205025185 2119 32-33 1.1038165817308823 2119 34-35 -0.15524532997149976 2119 36-37 -0.48992442962502025 2119 38-39 -2.7750332307712675 2119 40-41 -3.6177150791742605 2119 42-43 -3.3324776968025844 2119 44-45 -0.6812473754926245 2119 46-47 0.16464188365361565 2119 48-49 -3.707644303716492 2119 50-51 -1.1230613932805582 2119 52-53 -1.9939525238353966 2119 54-55 -3.9641161535787433 2119 56-57 -6.618413817728623 2119 58-59 -3.0478297987023772 2119 60-61 -0.35973917083315854 2119 62-63 -4.517542244081582 2119 64-65 0.017717820896891112 2119 66-67 1.2673072921886472 2119 68-69 -1.6070923482262458 2119 70-71 -0.4360169921238004 2119 72-73 -0.3727980691547046 2119 74-75 -0.04776456795969963 2119 76 2.1093325517610673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 3.0 24 8.0 25 22.0 26 57.0 27 116.0 28 198.0 29 303.0 30 444.0 31 644.0 32 875.0 33 1207.0 34 1452.0 35 1318.0 36 361.0 37 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 2.308015107007973 15.498671142817177 12.952860539935656 69.2404532102392 2 1.2169534200587495 3.678836200867254 83.27038746677857 11.833822912295426 3 1.566652678696321 16.491817037347882 73.75856763183663 8.182962652119178 4 0.8252902503846691 60.66582738844593 22.464680374877606 16.04420198629179 5 0.9232060428031893 29.416701636592528 65.78542453489999 3.874667785704294 6 2.126171492516436 68.07945167156245 11.106448454329277 18.68792838159183 7 2.028255700097916 29.06700237795496 8.504685970065744 60.400055951881384 8 2.014267729752413 67.22618548048678 15.344803469016647 15.41474332074416 9 1.790460204224367 15.358791439362149 4.126451251923346 78.72429710449013 10-11 2.112183522170933 38.264092880123094 39.70485382570989 19.918869771996086 12-13 8.322842355574206 5.860959574765701 40.467198209539795 45.3489998601203 14-15 11.281298083648062 9.910476989788782 9.910476989788782 68.89774793677438 16-17 37.85144775493076 32.962652119177505 1.95132186319765 27.234578262694082 18-19 37.87942369562177 16.97440201426773 1.7554902783606097 43.3906840117499 20-21 11.428171772275842 6.294586655476291 2.028255700097916 80.24898587214994 22-23 11.17638830605679 43.29276821933137 2.2660511959714644 43.26479227864037 24-25 37.788501888376 42.684291509302 2.2031053294167013 17.324101272905303 26-27 37.676598125611974 47.85984053713806 2.3499790180444817 12.113582319205483 28-29 5.028675339208281 54.140439222268846 2.5597985732270248 38.27108686529584 30-31 8.511679955238495 50.503566932438105 2.384948943908239 38.59980416841516 32-33 11.092460483983773 72.89131347041544 10.029374737725556 5.986851307875227 34-35 37.55070639250245 21.037907399636314 38.73968387187019 2.6717023359910477 36-37 37.85144775493076 33.053573926423276 19.499230661631 9.595747657014968 38-39 11.889774793677438 14.1418380193034 35.24968527066722 38.71870191635194 40-41 5.161561057490558 10.169254441180584 45.12519233459225 39.54399216673661 42-43 40.61407189816757 4.895789620926004 43.635473492796194 10.854664988110226 44-45 7.90320324520912 34.326479227864034 41.04769897887817 16.72261854804868 46-47 41.76807945167156 5.133585116799552 17.40103510980557 35.69730032172332 48-49 34.71114841236537 2.370960973562736 12.63113722198909 50.28675339208281 50-51 9.295006294586655 2.6367324101272906 45.90152468876766 42.1667366065184 52-53 7.735347601063086 3.273185060847671 75.62596167296125 13.365505665127989 54-55 4.406210658833403 3.1472933277381454 49.92306616309973 42.52342985032872 56-57 40.34830046160302 3.3361309274024338 20.43642467477969 35.87914393621486 58-59 7.455588194153028 16.87648622184921 64.8622184920968 10.805707091900965 60-61 34.07469576164498 49.46845712687089 13.113722198908938 3.3431249125751856 62-63 4.49013848090642 89.94264932158343 2.014267729752413 3.5529444677577287 64-65 1.2798992866135124 93.27178626381311 1.741502308015107 3.70681214155826 66-67 1.1889774793677437 91.27850048957896 2.0212617149251644 5.51126031612813 68-69 1.6226045600783328 85.57140858861379 2.9234858022100996 9.882501049097776 70-71 2.24506924045321 77.7801091061687 4.986711428171772 14.988110225206322 72-73 3.622884319485243 61.742901105049654 9.232060428031891 25.402154147433208 74-75 4.455168555042664 59.60973562736047 9.176108546649882 26.75898727094699 76 3.4270527346482025 62.652119177507345 8.812421317666805 25.10840677017765 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 1.0 23 0.5 24 3.0 25 5.0 26 3.5 27 7.0 28 7.5 29 3.0 30 2.0 31 3.5 32 8.5 33 11.0 34 9.5 35 21.5 36 35.5 37 36.0 38 136.5 39 638.0 40 1367.0 41 1671.5 42 1648.0 43 1366.5 44 826.5 45 435.0 46 302.0 47 232.0 48 123.5 49 65.5 50 46.0 51 38.0 52 28.0 53 27.5 54 29.0 55 27.0 56 17.5 57 13.5 58 17.0 59 16.0 60 10.0 61 2.5 62 0.0 63 0.0 64 1.5 65 1.5 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.289411106448455 #Duplication Level Percentage of deduplicated Percentage of total 1 74.90935460478607 14.449573366904461 2 11.022480058013052 4.252342985032872 3 4.713560551124003 2.7276542173730594 4 2.5380710659898478 1.9583158483704015 5 1.305293691080493 1.258917331095258 6 0.43509789702683105 0.5035669324381032 7 0.7251631617113851 0.9791579241852008 8 0.5076142131979695 0.7833263393481605 9 0.290065264684554 0.5035669324381032 >10 2.755620014503263 10.071338648762064 >50 0.36258158085569253 5.063645265072038 >100 0.290065264684554 12.407329696461042 >500 0.0725163161711385 9.357952161141418 >1k 0.0725163161711385 35.683312351377815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 2551 35.683312351377815 TruSeq Adapter, Index 5 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 669 9.357952161141418 TruSeq Adapter, Index 5 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 357 4.993705413344523 TruSeq Adapter, Index 5 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 217 3.035389564974122 RNA PCR Primer, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT 188 2.6297384249545392 No Hit CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 125 1.7484962931878585 RNA PCR Primer, Index 5 (95% over 24bp) TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT 87 1.2169534200587495 RNA PCR Primer, Index 5 (96% over 25bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT 87 1.2169534200587495 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 83 1.161001538676738 No Hit CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 53 0.741362428311652 TruSeq Adapter, Index 5 (95% over 24bp) CTTATACACATCTCCGAGCCCACGCGACACAAAGTGATCTCGTATGCCGT 52 0.7273744579661491 Illumina PCR Primer Index 5 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCG 40 0.5595188138201147 No Hit TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 37 0.5175549027836062 RNA PCR Primer, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGT 36 0.5035669324381032 No Hit ATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGTCTT 34 0.4755909917470975 Illumina PCR Primer Index 5 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCG 34 0.4755909917470975 No Hit CTTATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGT 30 0.41963911036508605 No Hit CTTATACACATCTCCGAGCCCACGAGACCCAAAGTGATCTCGTATGCCGT 28 0.39166316967408027 Illumina PCR Primer Index 5 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCC 26 0.36368722898307454 No Hit TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 26 0.36368722898307454 TruSeq Adapter, Index 5 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG 24 0.3357112882920688 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT 24 0.3357112882920688 No Hit CTTATACACATCTCCGAGCCCACGAGCCACAAAGTGATCTCGTATGCCGT 24 0.3357112882920688 TruSeq Adapter, Index 5 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACACACAGTGATCTCGTATGCCGT 21 0.2937473772555602 Illumina PCR Primer Index 5 (96% over 26bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGTCTT 20 0.27975940691005735 Illumina PCR Primer Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAAAAAGTGATCTCGTATGCCGT 20 0.27975940691005735 No Hit CTTATACACATCTCCGAGCACACGAGACACAAAGTGATCTCGTATGCCGT 19 0.2657714365645545 Illumina PCR Primer Index 5 (95% over 23bp) CTTATACACATCTCCGAGCCCAAGAGACACAAAGTGATCTCGTATGCCGT 19 0.2657714365645545 Illumina PCR Primer Index 5 (95% over 23bp) ACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCT 18 0.2517834662190516 RNA PCR Primer, Index 5 (96% over 28bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCC 16 0.22380752552804586 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAAATCGTATGCCGT 15 0.20981955518254303 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT 15 0.20981955518254303 No Hit CTTATACACATCTCCGAGCCCACGTGACACAAAGTGATCTCGTATGCCGT 15 0.20981955518254303 Illumina PCR Primer Index 5 (95% over 23bp) CTTATACACATATCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 13 0.18184361449153727 RNA PCR Primer, Index 5 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT 13 0.18184361449153727 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 12 0.1678556441460344 No Hit CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCC 12 0.1678556441460344 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATTTCGTATGCCGT 12 0.1678556441460344 No Hit ATACACATCTCCGAGCCCACGAGACACAACGTGATCTCGTATGCCGTCTT 12 0.1678556441460344 Illumina PCR Primer Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT 11 0.15386767380053154 No Hit CTTAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 11 0.15386767380053154 TruSeq Adapter, Index 5 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTCTGCCGT 11 0.15386767380053154 No Hit CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT 11 0.15386767380053154 TruSeq Adapter, Index 5 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGCTCTCGTATGCCGT 11 0.15386767380053154 No Hit ATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTGCTT 10 0.13987970345502868 TruSeq Adapter, Index 5 (96% over 32bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGTCTT 10 0.13987970345502868 Illumina PCR Primer Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCCCGTATGCCGT 10 0.13987970345502868 No Hit CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 10 0.13987970345502868 TruSeq Adapter, Index 5 (95% over 22bp) CTTATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 10 0.13987970345502868 No Hit CTTATACACATCTACGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 9 0.1258917331095258 TruSeq Adapter, Index 5 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACACCAAGTGATCTCGTATGCCGTCTT 9 0.1258917331095258 Illumina PCR Primer Index 5 (95% over 22bp) CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA 9 0.1258917331095258 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACATCGTATGCCGT 9 0.1258917331095258 No Hit CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGTC 8 0.11190376276402293 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGCGATCTCGTATGCCGT 8 0.11190376276402293 No Hit CTTATCACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 8 0.11190376276402293 TruSeq Adapter, Index 5 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCCT 8 0.11190376276402293 TruSeq Adapter, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACACGTATGCCGT 8 0.11190376276402293 No Hit CTTATACAAATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 8 0.11190376276402293 RNA PCR Primer, Index 5 (95% over 23bp) CATTAGAGTCTCTTGGAACAAATTGACAAACTGGTGTGTTTTCCTTCATT 8 0.11190376276402293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06993985172751434 0.0 2 0.0 0.0 0.0 0.06993985172751434 0.0 3 0.0 0.0 0.0 0.0839278220730172 0.0 4 0.0 0.0 0.0 0.09791579241852007 0.0 5 0.0 0.0 0.0 0.09791579241852007 0.0 6 0.0 0.0 0.0 0.09791579241852007 0.0 7 0.0 0.0 0.0 0.09791579241852007 0.0 8 0.0 0.0 0.0 0.09791579241852007 0.0 9 0.0 0.0 0.0 0.09791579241852007 0.0 10 0.0 0.0 0.0 0.09791579241852007 0.0 11 0.0 0.0 0.0 0.11190376276402295 0.0 12 0.0 0.0 0.0 0.18184361449153727 0.0 13 0.0 0.0 0.0 0.18184361449153727 0.0 14 0.0 0.0 0.0 0.19583158483704013 0.0 15 0.0 0.0 0.0 0.2517834662190516 0.0 16 0.0 0.0 0.0 0.2937473772555602 0.0 17 0.0 0.0 0.0 0.3357112882920688 0.0 18 0.0 0.0 0.0 0.4755909917470975 0.0 19 0.0 0.0 0.0 0.6434466358931319 0.0 20 0.0 0.0 0.0 0.7133864876206463 0.0 21 0.0 0.0 0.0 0.7553503986571548 0.0 22 0.0 0.0 0.0 0.7833263393481605 0.0 23 0.0 0.0 0.0 0.8113022800391663 0.0 24 0.0 0.0 0.0 0.8113022800391663 0.0 25 0.0 0.0 0.0 0.8113022800391663 0.0 26 0.0 0.0 0.0 0.8113022800391663 0.0 27 0.0 0.0 0.0 0.839278220730172 0.0 28 0.0 0.0 0.0 0.839278220730172 0.0 29 0.0 0.0 0.0 0.8532661910756749 0.0 30 0.0 0.0 0.0 0.8532661910756749 0.0 31 0.0 0.0 0.0 0.8812421317666806 0.0 32 0.0 0.0 0.0 0.9791579241852008 0.0 33 0.0 0.0 0.0 1.0071338648762065 0.0 34 0.0 0.0 0.0 1.063085746258218 0.0 35 0.0 0.0 0.0 1.0770737166037208 0.0 36 0.0 0.0 0.0 1.2029654497132467 0.0 37 0.0 0.0 0.0 1.3428451531682752 0.0 38 0.0 0.0 0.0 1.580640649041824 0.0 39 0.0 0.0 0.0 1.8604000559518814 0.0 40 0.0 0.0 0.0 2.014267729752413 0.0 41 0.0 0.0 0.0 2.1401594628619387 0.0 42 0.0 0.0 0.0 2.39194292908099 0.0 43 0.0 0.0 0.0 2.5178346621905163 0.0 44 0.0 0.0 0.0 2.6856903063365505 0.0 45 0.0 0.0 0.0 2.7836060987550706 0.0 46 0.0 0.0 0.0 2.923485802210099 0.0 47 0.0 0.0 0.0 3.063365505665128 0.0 48 0.0 0.0 0.0 3.1472933277381454 0.0 49 0.0 0.0 0.0 3.3291369422296824 0.0 50 0.0 0.0 0.0 3.4130647643026997 0.0 51 0.0 0.0 0.0 3.4690166456847114 0.0 52 0.0 0.0 0.0 3.5809204084487343 0.0 53 0.0 0.0 0.0 3.692824171212757 0.0 54 0.0 0.0 0.0 3.692824171212757 0.0 55 0.0 0.0 0.0 3.9026437263953 0.0 56 0.0 0.0 0.0 4.154427192614351 0.0 57 0.0 0.0 0.0 4.1963911036508605 0.0 58 0.0 0.0 0.0 4.3502587774513914 0.0 59 0.0 0.0 0.0 4.378234718142398 0.0 60 0.0 0.0 0.0 4.406210658833404 0.0 61 0.0 0.0 0.0 4.518114421597426 0.0 62 0.0 0.0 0.0 4.588054273324941 0.0 63 0.0 0.0 0.0 4.671982095397958 0.0 64 0.0 0.0 0.0 4.685970065743461 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGAG 15 0.0021346959 70.00001 20 CACACGA 15 0.0021346959 70.00001 19 CTTTACA 15 0.0021346959 70.00001 1 AGCCACA 15 0.0021346959 70.00001 25 AAATAAA 15 0.0021346959 70.00001 70 TTACACA 15 0.0021346959 70.00001 3 GAGCCAC 20 7.399345E-5 70.00001 24 CACGAGC 15 0.0021346959 70.00001 21 GAGCACA 15 0.0021346959 70.00001 16 ACGAGCC 15 0.0021346959 70.00001 22 CGAGCCA 20 7.399345E-5 70.00001 23 CGAGCAC 15 0.0021346959 70.00001 15 CCGAGCA 15 0.0021346959 70.00001 14 GCCACAA 15 0.0021346959 70.00001 26 AGCACAC 15 0.0021346959 70.00001 17 TTTACAC 15 0.0021346959 70.00001 2 GCACACG 15 0.0021346959 70.00001 18 ACCAAGT 20 0.0066722734 52.500004 29 CACCAAG 20 0.0066722734 52.500004 28 AAAATAA 20 0.0066722734 52.500004 69 >>END_MODULE