FastQCFastQC Report
Wed 25 May 2016
SRR1780715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93994
Sequences flagged as poor quality0
Sequence length50
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA1460.15532906355724835No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1160.1234121326893206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAC301.3019948E-436.6187442
CTTTCCA250.002350710635.172721
CTTTACA1250.035.172721
TTTCCAC250.002356867335.1539962
TCTTTAT401.8119215E-532.9744261
AAATCTG353.2301695E-431.370798
GTGTGAC300.0056805129.37319811
GGAATGA300.00577008729.2794065
ATTACAC300.00577008729.2794064
TCTAACG300.00577008729.2794068
TATTACA300.00577008729.2794063
TTGCAGA300.00577008729.2794069
TGTACAC300.00577008729.2794063
CGGTGGT852.6011548E-1028.43337643
TTTACAC1550.028.3499972
CTTTATA554.947482E-627.9634062
CGAAACT959.495125E-1025.4403925
CGGAGTG701.1265456E-625.10999532
CTATACA801.2928285E-724.730821
GTAGATA450.001373387624.4907438
Produced by FastQC (version 0.11.5)