##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780715_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93994 Sequences flagged as poor quality 0 Sequence length 50 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.188246058259036 16.0 10.0 28.0 7.0 31.0 2 19.739462093325105 16.0 10.0 28.0 7.0 31.0 3 19.911909270804518 20.0 10.0 29.0 7.0 31.0 4 21.88509904887546 22.0 10.0 32.0 7.0 35.0 5 21.67642615486095 20.0 10.0 32.0 7.0 35.0 6 21.62277379407196 20.0 10.0 32.0 8.0 35.0 7 21.64604123667468 20.0 10.0 32.0 8.0 35.0 8 21.603868332021193 19.0 10.0 32.0 8.0 35.0 9 21.905366299976595 19.0 10.0 33.0 8.0 38.0 10 21.84002170351299 19.0 10.0 32.0 8.0 38.0 11 21.86966189331234 19.0 10.0 32.0 8.0 38.0 12 21.66567014915846 18.0 10.0 32.0 8.0 38.0 13 21.559312296529566 18.0 10.0 32.0 8.0 38.0 14 21.860661318807583 18.0 10.0 32.0 8.0 39.0 15 21.72707832414835 18.0 10.0 32.0 8.0 39.0 16 21.643019767219183 17.0 10.0 32.0 8.0 39.0 17 21.638445007128116 17.0 10.0 32.0 8.0 39.0 18 21.480200863884928 17.0 10.0 32.0 8.0 38.0 19 21.50167031938209 18.0 10.0 32.0 7.0 39.0 20 21.544502840606846 18.0 10.0 32.0 7.0 39.0 21 21.337606655743983 18.0 9.0 32.0 7.0 39.0 22 21.4032385045854 18.0 9.0 32.0 7.0 39.0 23 21.24441985658659 18.0 9.0 32.0 7.0 39.0 24 20.93980466838309 18.0 8.0 32.0 7.0 38.0 25 20.874651573504693 18.0 8.0 31.0 7.0 38.0 26 20.628284784135158 17.0 8.0 31.0 7.0 38.0 27 20.38892908057961 17.0 8.0 31.0 7.0 38.0 28 20.256601485201184 17.0 8.0 30.0 7.0 38.0 29 20.04475817605379 16.0 8.0 30.0 7.0 38.0 30 19.878268825669725 16.0 8.0 30.0 7.0 38.0 31 19.764559439964252 16.0 8.0 30.0 7.0 38.0 32 19.546524246228483 16.0 8.0 30.0 7.0 38.0 33 19.48668000085112 16.0 8.0 30.0 7.0 38.0 34 19.137040662169927 15.0 8.0 30.0 7.0 37.0 35 18.976721918420324 15.0 8.0 29.0 7.0 37.0 36 18.869938506713194 15.0 8.0 29.0 7.0 37.0 37 18.644030470030003 15.0 8.0 28.0 7.0 37.0 38 18.661074111113475 15.0 8.0 28.0 7.0 37.0 39 18.382652084175586 15.0 8.0 28.0 7.0 37.0 40 17.830648764814775 13.0 8.0 27.0 7.0 37.0 41 18.030757282379728 14.0 8.0 27.0 7.0 37.0 42 18.04489648275422 14.0 8.0 28.0 7.0 37.0 43 18.059184628806094 13.0 8.0 28.0 7.0 37.0 44 18.039087601336256 13.0 8.0 28.0 7.0 37.0 45 18.079068876736812 13.0 8.0 28.0 7.0 36.0 46 17.873055726961294 13.0 8.0 27.0 7.0 36.0 47 17.80904100261719 13.0 8.0 27.0 7.0 36.0 48 17.708970785369278 13.0 8.0 26.0 7.0 36.0 49 17.445688022639743 13.0 8.0 26.0 7.0 35.0 50 17.442985722492924 13.0 8.0 26.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.6428700721331957 1101 2 -0.6395595617020007 1101 3 -1.175999511348845 1101 4 -1.203126742780352 1101 5 -0.7120931572938822 1101 6 -1.8094451427546439 1101 7 -1.9701549134336105 1101 8 -1.384625233378049 1101 9 -2.6888647584466234 1101 10 -1.8499214820542917 1101 11 -1.7095216693739559 1101 12 -2.169287016702885 1101 13 -1.711782245169033 1101 14 -1.3279841180572056 1101 15 -1.691139717103038 1101 16 -1.7583621650815573 1101 17 -2.3528367885623744 1101 18 -2.4102730359062967 1101 19 -2.4751284790155523 1101 20 -2.310715453619707 1101 21 -1.4396350709408239 1101 22 -1.893869220123353 1101 23 -0.9967195716760564 1101 24 -1.4019047183446247 1101 25 -2.606501571941074 1101 26 -1.9366364399754232 1101 27 -1.8911432586032184 1101 28 -0.5119849664546727 1101 29 -1.0238191813315716 1101 30 -2.2929559667067316 1101 31 -2.8525742799037523 1101 32 -2.012290418505639 1101 33 -1.6467803555515488 1101 34 -2.0091837412614275 1101 35 -2.320294951255363 1101 36 -2.251306117741418 1101 37 -1.75243145330219 1101 38 -1.6602030131046952 1101 39 -1.8312774209581981 1101 40 -2.0916268418570265 1101 41 -1.6784437650648272 1101 42 -1.7496801563489974 1101 43 -2.722564535375234 1101 44 -3.1911735580866356 1101 45 -2.5243912503516075 1101 46 -1.9517138852671554 1101 47 -2.5466750390219524 1101 48 -2.7137498411630894 1101 49 -2.145485473852048 1101 50 -1.3589132264148027 1102 1 0.5674531812557504 1102 2 0.38186900972657156 1102 3 0.8445427201422895 1102 4 1.8955176784918066 1102 5 0.8087097624141464 1102 6 2.196185722730238 1102 7 -0.5840756642156748 1102 8 0.21162085629869765 1102 9 -0.614672891918989 1102 10 1.0361056295202644 1102 11 0.4308328666010155 1102 12 1.0629340469050383 1102 13 1.1648079529540105 1102 14 1.9185226598364338 1102 15 0.694105329820843 1102 16 0.8795419016546262 1102 17 0.23438948880988875 1102 18 0.1047947430300944 1102 19 0.07674847614607572 1102 20 0.7058643169746617 1102 21 -0.20157459127450394 1102 22 -0.041470888527939564 1102 23 -0.6069906874216251 1102 24 0.4447584724791511 1102 25 -0.5340302476449317 1102 26 -0.029754271049458936 1102 27 0.9117243117825993 1102 28 0.6004759303127933 1102 29 0.640622945884278 1102 30 0.20167385602528043 1102 31 0.8690107044549542 1102 32 -0.1962320243450577 1102 33 -0.5438085098789713 1102 34 0.03815723892626721 1102 35 -0.13468494082783522 1102 36 0.1687668749593101 1102 37 -0.26437097363587014 1102 38 -0.43846161581585363 1102 39 -0.520172102918572 1102 40 0.03652748556737251 1102 41 0.8783341285743802 1102 42 0.40772338901075145 1102 43 0.47660126233071054 1102 44 0.5566887985348465 1102 45 0.38655765475787973 1102 46 1.1967741230748672 1102 47 -0.5715446949135057 1102 48 0.19652127458248003 1102 49 0.29919648652334274 1102 50 0.36965820215662504 1103 1 -0.25331682887595974 1103 2 -0.20693544822682242 1103 3 -0.21026984318091024 1103 4 -0.3302168706469999 1103 5 0.03885160638577645 1103 6 -0.3622742570063373 1103 7 -0.6836197371640012 1103 8 -1.538531119385194 1103 9 -0.16984006517126815 1103 10 -0.19702506956787857 1103 11 -0.5709908416056706 1103 12 -0.4315948284748998 1103 13 0.8269660076652556 1103 14 -0.5051855468504982 1103 15 1.4075298485645078 1103 16 0.00340208402544917 1103 17 0.6042476448382565 1103 18 0.15813820807568746 1103 19 0.08764006682489978 1103 20 0.09421284787638484 1103 21 0.18758447660796662 1103 22 -0.3422307669473952 1103 23 0.11261417580026034 1103 24 0.6480512789634467 1103 25 -1.1606766508870798 1103 26 0.398715840399376 1103 27 -0.8495218888253042 1103 28 0.8425731947504822 1103 29 -0.2860234573578708 1103 30 -0.6286706221915388 1103 31 1.0979874015167574 1103 32 1.162633223881894 1103 33 0.37843059853035044 1103 34 -0.3318528927859923 1103 35 1.001080003447747 1103 36 1.8343697118387432 1103 37 1.5911001509841896 1103 38 1.4820551015093777 1103 39 -0.26338385570479517 1103 40 0.2435183670263399 1103 41 0.07757425015492458 1103 42 0.6880172086662739 1103 43 0.6640379391290878 1103 44 0.6877931551711178 1103 45 -0.8219529835399904 1103 46 0.07701728416909148 1103 47 0.5125991754005774 1103 48 -0.241523304951464 1103 49 0.3368357975669092 1103 50 0.9886045040816498 1104 1 0.06583210729425204 1104 2 0.38171703404267987 1104 3 0.6195173908616418 1104 4 0.14496043431753947 1104 5 -0.6987370460964932 1104 6 -0.5963168101978269 1104 7 -0.428300588227831 1104 8 -0.16264459456249725 1104 9 -0.36132942687339664 1104 10 -0.04099670077355455 1104 11 -0.13836672813049233 1104 12 0.8095399232981499 1104 13 0.8907957948992973 1104 14 0.09055913400056781 1104 15 0.49972843012479373 1104 16 0.7197141407630383 1104 17 -0.23972398636741588 1104 18 0.8460814704870359 1104 19 0.4280655987397921 1104 20 0.9949220677345387 1104 21 1.2159532709342074 1104 22 0.2676983110667912 1104 23 -0.05050639157562742 1104 24 0.7828030520130937 1104 25 0.6691105831554722 1104 26 -0.21121323761480681 1104 27 -1.2608694065557984 1104 28 -0.058136025107671685 1104 29 -0.15836388288978753 1104 30 -0.45845785623409085 1104 31 -1.5828636623130308 1104 32 -0.6955228044868988 1104 33 0.14438804533886085 1104 34 -0.5020656587434367 1104 35 -0.6088490745664359 1104 36 -1.3571196498633853 1104 37 -0.41599204759737063 1104 38 -0.6101434800509082 1104 39 -0.029341302513305578 1104 40 -0.0756305691438719 1104 41 -4.399342422374275E-4 1104 42 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28 -0.034080054243190716 1105 29 -0.5705739653126329 1105 30 -0.22824892389749962 1105 31 0.07364390908815821 1105 32 0.46457878646575423 1105 33 0.3973111812407204 1105 34 0.38699893776796657 1105 35 -0.390476446619342 1105 36 -1.0555574493650113 1105 37 -0.5348342940991913 1105 38 0.4489901216358838 1105 39 -0.11708329982976906 1105 40 0.4384579874978769 1105 41 0.09281289305314289 1105 42 0.010233041520404385 1105 43 -1.182665146935701 1105 44 -0.3630023211562765 1105 45 -1.2701991019988768 1105 46 -0.7970482707475277 1105 47 -0.8896914130602234 1105 48 -0.7839806559194535 1105 49 -0.1706876833608284 1105 50 -0.4332375507391255 1106 1 0.03482769863027002 1106 2 -0.1463163184587586 1106 3 0.129484805084374 1106 4 0.16829760127172833 1106 5 -1.1129118592074754 1106 6 -1.2291038525593372 1106 7 -1.207627787767798 1106 8 -0.9314293367514956 1106 9 -0.9135145907606876 1106 10 -1.254241860827225 1106 11 -1.8533370175688688 1106 12 -1.1859076519366596 1106 13 -1.0790144408683844 1106 14 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1106 50 1.0127083952068183 1107 1 -0.9869246989285791 1107 2 -1.452462787508452 1107 3 -0.8775770955259112 1107 4 -1.2233763307247827 1107 5 -1.509032173069727 1107 6 -1.9918327104494864 1107 7 -1.6584996273493147 1107 8 -1.282388360291165 1107 9 -1.5061475462968694 1107 10 -1.6675810064244345 1107 11 -2.0843745066708728 1107 12 -2.11079867198897 1107 13 -1.838648268244146 1107 14 -1.0372377088271634 1107 15 -0.764650430547821 1107 16 -0.46907560986610974 1107 17 -1.170449220867532 1107 18 -1.9350670081219796 1107 19 -1.9036662703977036 1107 20 -0.8466702452373198 1107 21 -1.5430492456523872 1107 22 -1.0163556811546677 1107 23 -1.3943178763617148 1107 24 -0.5361171035577144 1107 25 -0.678269878981336 1107 26 -1.0670814599895522 1107 27 -1.0691512642542662 1107 28 -0.4315517194567917 1107 29 -0.9261973306134195 1107 30 -1.5156072499655053 1107 31 -0.09043630015334259 1107 32 -0.783328798538605 1107 33 -0.34035228868081546 1107 34 -1.4494003647603684 1107 35 -0.9853761850674658 1107 36 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2213 16 0.25657929379269007 2213 17 1.2501613008904258 2213 18 1.1698474755324924 2213 19 1.5386372585046573 2213 20 1.2079736170705395 2213 21 0.7101224365299039 2213 22 0.054741959027190035 2213 23 1.481456100689595 2213 24 0.7197824417984222 2213 25 1.0551356200444246 2213 26 0.38453144323625565 2213 27 0.8713120394911336 2213 28 0.6824989408592934 2213 29 1.990191498137392 2213 30 1.217062158997475 2213 31 2.87955720493434 2213 32 2.3299424722992406 2213 33 1.035529936909139 2213 34 0.9539769896453478 2213 35 0.9209238798816557 2213 36 0.8711158682479017 2213 37 1.135581087725587 2213 38 0.8376170035513582 2213 39 1.6332794790564975 2213 40 0.6182149256440752 2213 41 0.45441274794158915 2213 42 1.607004309432611 2213 43 1.4823538989558287 2213 44 1.8679064399538277 2213 45 2.355685171536404 2213 46 1.3848853407162842 2213 47 1.8699222274258993 2213 48 1.7208261643236114 2213 49 2.0291581356125086 2213 50 1.8424290554643932 2214 1 -0.542070628268057 2214 2 -0.027314663742817658 2214 3 -0.2105593206740366 2214 4 -0.12352994055581235 2214 5 0.6940839120740101 2214 6 1.1676142941588097 2214 7 0.7475569888455524 2214 8 0.5558385433735253 2214 9 0.46049717610822327 2214 10 1.4163777383638028 2214 11 0.05804375095475933 2214 12 0.6404850673132039 2214 13 -0.15117844160381466 2214 14 -0.620252850367649 2214 15 -0.658615758614534 2214 16 -0.5541315677331262 2214 17 -0.2686717356799093 2214 18 -0.45540933860255706 2214 19 0.5172246666222655 2214 20 -0.428149288467516 2214 21 -0.643071189913961 2214 22 -0.006436874922499669 2214 23 0.18382563910898853 2214 24 -0.033812956092276636 2214 25 -0.19729555376738261 2214 26 -0.8535637948589816 2214 27 0.03859506008192071 2214 28 -0.2570070628844867 2214 29 0.8066093404421011 2214 30 0.7761636519436479 2214 31 0.6105073030944084 2214 32 0.02961831579099794 2214 33 0.15857244250198121 2214 34 0.8667286674976964 2214 35 -0.4816237163380386 2214 36 0.14121585455114882 2214 37 0.4931120195614085 2214 38 -0.4619310035709532 2214 39 -0.48989999407503504 2214 40 -0.01585346681357791 2214 41 -0.287312129929024 2214 42 0.6366349536366016 2214 43 0.544628473215063 2214 44 0.5253962815280424 2214 45 0.6668297636014202 2214 46 0.9233047353197641 2214 47 2.0614485023654048 2214 48 2.1261131113171707 2214 49 0.5383121327818472 2214 50 0.32135888242873634 2215 1 0.30598701584221644 2215 2 0.1235983330348418 2215 3 0.6883397126234918 2215 4 -0.16331690797435883 2215 5 -0.38602707969111805 2215 6 0.3809601588204643 2215 7 0.5578416290174104 2215 8 -0.3295445572351383 2215 9 -1.3406127415430475 2215 10 0.38836126861800935 2215 11 -0.08288893791026197 2215 12 0.47252051307045306 2215 13 0.16725156197656688 2215 14 0.524349727505605 2215 15 -0.14724805363780646 2215 16 0.7714591948877114 2215 17 0.8186000151256785 2215 18 -0.2224608960676484 2215 19 0.8032522355445693 2215 20 0.31169138464383295 2215 21 0.6566960854172237 2215 22 0.4000704648555171 2215 23 0.48887397423251144 2215 24 0.4972020815017082 2215 25 0.5430374215280018 2215 26 1.6345314432362557 2215 27 1.5760100260683139 2215 28 0.2888217949744494 2215 29 0.187427457162471 2215 30 0.9520497958749026 2215 31 1.0363039375376601 2215 32 0.8774521861812588 2215 33 0.5708531442563647 2215 34 0.4785707727608539 2215 35 0.15290904413457085 2215 36 0.5253458223277327 2215 37 0.10705759779269997 2215 38 1.0715007901991846 2215 39 0.3382865436979685 2215 40 -0.02775822871834066 2215 41 -0.3182824127790056 2215 42 -0.5050585658764675 2215 43 0.0038569014284526304 2215 44 0.02924518951228805 2215 45 -0.026600239978961326 2215 46 0.17948088999215983 2215 47 0.608530493056417 2215 48 0.5374987181915003 2215 49 0.5273919752451484 2215 50 -0.03654480536217264 2216 1 1.0696509834535526 2216 2 0.7514660793236416 2216 3 1.1223815479811172 2216 4 1.3489975319716585 2216 5 1.5151200188244012 2216 6 1.9533285798730944 2216 7 1.4763638962238872 2216 8 1.9914743361521765 2216 9 2.2029095256634292 2216 10 1.6903180837327199 2216 11 1.6480489838171337 2216 12 1.0138497978207894 2216 13 1.28550392364998 2216 14 1.3038706451844178 2216 15 0.9023470880626014 2216 16 1.119468641581367 2216 17 1.5702869247073217 2216 18 1.9980181862535389 2216 19 2.349642249039846 2216 20 2.8283917895021347 2216 21 1.5288283391284239 2216 22 2.21805292099587 2216 23 2.6585770781461413 2216 24 1.6539159816366613 2216 25 0.9549807413660574 2216 26 1.3653006740054856 2216 27 -0.07783612777783944 2216 28 0.42849790833477286 2216 29 1.6629672682285985 2216 30 1.9628460171974424 2216 31 2.7701096055538557 2216 32 2.576844898731867 2216 33 2.229085263014799 2216 34 2.3282671290361563 2216 35 1.476120919978026 2216 36 1.8713309775234137 2216 37 0.8057755465106489 2216 38 2.258241680887444 2216 39 1.372868190121718 2216 40 1.6132674123073016 2216 41 1.8301656304058795 2216 42 2.3073570886444514 2216 43 1.5718393575688054 2216 44 2.087274879120926 2216 45 1.1916858598860856 2216 46 2.4328547091554533 2216 47 3.119664096295285 2216 48 2.7912099192711217 2216 49 2.157621394948251 2216 50 2.0257021582005805 >>END_MODULE >>Per sequence quality scores fail #Quality Count 3 1.0 4 1.0 5 3.0 6 20.0 7 404.0 8 2689.0 9 6867.0 10 6219.0 11 5086.0 12 4647.0 13 4634.0 14 4626.0 15 4555.0 16 4223.0 17 3985.0 18 3694.0 19 3284.0 20 2928.0 21 2833.0 22 2530.0 23 2403.0 24 2265.0 25 2096.0 26 2053.0 27 1943.0 28 1833.0 29 1789.0 30 1670.0 31 1518.0 32 1454.0 33 1337.0 34 1253.0 35 1200.0 36 1270.0 37 1217.0 38 1337.0 39 4127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.66882874822933 19.45234367511263 17.225293159088732 29.65353441756931 2 22.431239027504386 17.68686492525403 39.578656168537535 20.303239878704048 3 22.30887077898589 20.880056173798327 35.36608719705513 21.44498585016065 4 19.387407706874907 20.91942038853544 29.09334638381173 30.599825520777923 5 20.023831561924823 30.665049524964626 30.38417754513634 18.926941367974212 6 27.297487073643 27.587931144541137 21.870544928399685 23.244036853416176 7 25.925059046322108 29.993403834287296 18.90014256229121 25.18139455709939 8 23.504691788837587 33.212758261165604 20.38853543843224 22.894014511564567 9 22.795314544700137 23.358122413371206 17.885374442777653 35.961188599151 10 21.279470593241978 29.106732487871305 26.922504042897266 22.691292875989447 11 31.960890704626944 24.082644452246445 22.08675114104242 21.869713702084198 12 23.913482918940772 25.961507771855352 23.217686423457064 26.907322885746808 13 28.120478338582007 20.82198484977445 24.39462933015576 26.662907481487785 14 32.1963571367776 22.27955570686867 19.87722359349732 25.646863562856414 15 26.06392033364542 26.920376202229978 18.875010639203335 28.14069282492127 16 28.441735572414967 22.730372517261955 20.715412155826442 28.112479754496633 17 33.88905438760745 22.45616648225381 18.621797599795727 25.032981530343008 18 27.46403835908364 20.818327117741077 19.896643580181138 31.820990942994136 19 28.211605696256502 21.502728745629742 23.275347488701286 27.010318069412463 20 33.92876186413565 21.174149514770182 20.57374426788952 24.32334435320465 21 27.47410970446134 23.336995125905226 19.109224517656596 30.079670651976837 22 29.233323753871698 20.643739821822013 18.534129493033603 31.588806931272686 23 35.050328786363345 22.641569662275753 18.63122725628312 23.67687429507778 24 27.582129617793683 27.71528399164856 18.510588435808938 26.191997954748818 25 31.529930177111716 22.3443886239782 18.224199591280655 27.90148160762943 26 32.313439189476824 24.96488016687242 18.332552892597164 24.389127751053593 27 29.112967594317034 26.42153993508221 19.62858511147768 24.836907359123078 28 32.11765832685943 25.009843881362603 19.38765736907638 23.484840422701588 29 30.01170710940826 24.56257982120051 20.510855683269476 24.914857386121753 30 31.235160716757342 23.649159417820982 20.27724838431481 24.838431481106866 31 31.108107245111917 22.302640682043148 21.222313284301723 25.36693878854321 32 30.102594665928777 22.23452033800898 21.692811987824864 25.97007300823737 33 34.6388812022393 20.83271248855872 20.51980672215245 24.008599587049535 34 30.674911924047134 21.491596862260916 23.038114802081893 24.79537641161006 35 32.81396338867603 22.561728395061728 20.863133248190717 23.76117496807152 36 33.99150584904255 20.127305821367365 23.403621190671338 22.47756713891875 37 30.59002575509248 20.723271109597498 24.324727017304866 24.361976118005153 38 32.94272912076801 19.827154975148208 23.877944165948254 23.35217173813553 39 31.187934392728277 20.43149234191564 25.416963801049462 22.96360946430662 40 25.96358830050311 32.474204826468835 22.375714163895285 19.18649270913277 41 31.772607288828336 17.260941757493185 25.245870231607633 25.720580722070846 42 25.810503012112314 20.77293143453179 29.609170445111438 23.80739510824446 43 26.523488218641578 17.059023860709647 28.88614546305954 27.531342457589236 44 27.15325515568136 17.255836720795116 29.644369719284057 25.946538404239472 45 26.228304476913088 17.188281491098316 32.986772514924816 23.596641517063773 46 26.060032133470944 19.462030367194068 28.276390411031784 26.201547088303208 47 30.9841769794737 19.115315449524882 28.200517147812764 21.699990423188652 48 30.67313626593458 21.358190214731106 25.896486411713383 22.072187107620934 49 28.892151855635 21.19190500510725 26.600272386789243 23.315670752468506 50 29.895930875968336 22.763258704350047 23.432578530688687 23.908231888992933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 4.0 8 7.0 9 8.0 10 9.0 11 10.5 12 12.0 13 15.5 14 19.0 15 33.0 16 47.0 17 55.0 18 63.0 19 80.5 20 98.0 21 163.5 22 229.0 23 258.5 24 288.0 25 379.0 26 470.0 27 571.5 28 673.0 29 793.5 30 914.0 31 1053.0 32 1192.0 33 1431.0 34 1670.0 35 1943.0 36 2216.0 37 2469.5 38 2723.0 39 2915.5 40 3108.0 41 3379.0 42 3650.0 43 3856.0 44 4062.0 45 4559.0 46 5056.0 47 5484.0 48 5912.0 49 6118.5 50 6325.0 51 6547.0 52 6769.0 53 6677.5 54 6586.0 55 6348.5 56 6111.0 57 5913.5 58 5716.0 59 5436.5 60 5157.0 61 4769.0 62 4381.0 63 4000.5 64 3620.0 65 3372.5 66 3125.0 67 2916.5 68 2708.0 69 2521.0 70 2334.0 71 2168.0 72 2002.0 73 1815.5 74 1629.0 75 1529.0 76 1429.0 77 1284.0 78 1139.0 79 1029.5 80 920.0 81 777.5 82 635.0 83 501.5 84 368.0 85 316.5 86 265.0 87 218.0 88 171.0 89 126.0 90 81.0 91 60.0 92 39.0 93 32.0 94 25.0 95 18.5 96 12.0 97 12.0 98 12.0 99 14.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10958146264655191 2 0.009575079260378322 3 0.0 4 0.0 5 0.0010638976955975913 6 0.0 7 0.0 8 0.0 9 0.0010638976955975913 10 0.0021277953911951826 11 0.0010638976955975913 12 0.0010638976955975913 13 0.0021277953911951826 14 0.0021277953911951826 15 0.0021277953911951826 16 0.15532906355724835 17 0.0021277953911951826 18 0.15320126816605315 19 0.18937378981637126 20 0.2383130838138605 21 0.24788816307423878 22 0.04574760091069643 23 0.012766772347171097 24 0.12553992808051578 25 0.04468370321509884 26 0.03191693086792774 27 0.03085303317233015 28 0.028725237781134963 29 0.036172521650318104 30 0.07553673638742898 31 0.043619805519501245 32 0.03404472625912292 33 0.038300317041513286 34 0.043619805519501245 35 0.036172521650318104 36 0.05000319169308679 37 0.03404472625912292 38 0.03936421473711088 39 0.043619805519501245 40 0.18937378981637126 41 0.04468370321509884 42 0.04255590782390366 43 0.03404472625912292 44 0.02127795391195183 45 0.024469646998744602 46 0.011702874651573504 47 0.018086260825159052 48 0.01702236312956146 49 0.010638976955975914 50 0.019150158520756643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 93994.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.97708364363683 #Duplication Level Percentage of deduplicated Percentage of total 1 98.42947855881562 89.54826903844926 2 0.7671348216060716 1.3958337766240398 3 0.1988001824284027 0.5425878247547716 4 0.05145416486382187 0.18724599442517606 5 0.04326827499912294 0.19682107368555438 6 0.03742121081005227 0.20426835755473755 7 0.029235320945353336 0.18618209672957847 8 0.033912972296609875 0.2468242653786412 9 0.021049431080654404 0.1723514266868098 >10 0.3730426952627086 6.199331872247165 >50 0.012863541215955468 0.8415430772176947 >100 0.002338825675628267 0.2787411962465689 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA 146 0.15532906355724835 No Hit CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 116 0.1234121326893206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.09468689490818563 0.0 2 0.0 0.0 0.0 0.09575079260378322 0.0 3 0.0 0.0 0.0 0.13830670042768686 0.0 4 0.0 0.0 0.0 0.1702236312956146 0.0 5 0.0 0.0 0.0 0.18192650594718812 0.0 6 0.0 0.0 0.0 0.1829904036427857 0.0 7 0.0 0.0 0.0 0.1840543013383833 0.0 8 0.0 0.0 0.0 0.18618209672957847 0.0 9 0.0 0.0 0.0 0.18937378981637126 0.0 10 0.0 0.0 0.0 0.2117156414239207 0.0 11 0.0 0.0 0.0 0.21916292529310383 0.0 12 0.0 0.0 0.0 0.32980828563525333 0.0 13 0.0 0.0 0.0 0.33831946720003403 0.0 14 0.0 0.0 0.0 0.3585335234163883 0.0 15 0.0 0.0 0.0 0.38513096580632805 0.0 16 0.0 0.0 0.0 0.4170478966742558 0.0 17 0.0 0.0 0.0 0.4691788837585378 0.0 18 0.0 0.0 0.0 0.6298274357937741 0.0 19 0.0 0.0 0.0 0.8713322126944273 0.0 20 0.0 0.0 0.0 0.973466391471796 0.0 21 0.0 0.0 0.0 1.0255973785560781 0.0 22 0.0 0.0 0.0 1.0968785241611168 0.0 23 0.0 0.0 0.0 1.1628401812881672 0.0 24 0.0 0.0 0.0 1.187309828286912 0.0 25 0.0 0.0 0.0 1.1905015213737047 0.0 26 0.0 0.0 0.0 1.1915654190693024 0.0 27 0.0 0.0 0.0 1.2426325084579868 0.0 28 0.0 0.0 0.0 1.2660382577611338 0.0 29 0.0 0.0 0.0 1.2990190863246591 0.0 30 0.0 0.0 0.0 1.3341277102793796 0.0 31 0.0 0.0 0.0 1.4171117305359917 0.0 32 0.0 0.0 0.0 1.5117986254441773 0.0 33 0.0 0.0 0.0 1.57244079409324 0.0 34 0.0 0.0 0.0 1.7118113922165246 0.0 35 0.0 0.0 0.0 1.8139455709938932 0.0 36 0.0 0.0 0.0 2.076728301806498 0.0 37 0.0 0.0 0.0 2.368236270400238 0.0 38 0.0 0.0 0.0 2.8267761772028 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAACAC 30 1.3019948E-4 36.618744 2 CTTTCCA 25 0.0023507106 35.17272 1 CTTTACA 125 0.0 35.17272 1 TTTCCAC 25 0.0023568673 35.153996 2 TCTTTAT 40 1.8119215E-5 32.974426 1 AAATCTG 35 3.2301695E-4 31.37079 8 GTGTGAC 30 0.00568051 29.373198 11 GGAATGA 30 0.005770087 29.279406 5 ATTACAC 30 0.005770087 29.279406 4 TCTAACG 30 0.005770087 29.279406 8 TATTACA 30 0.005770087 29.279406 3 TTGCAGA 30 0.005770087 29.279406 9 TGTACAC 30 0.005770087 29.279406 3 CGGTGGT 85 2.6011548E-10 28.433376 43 TTTACAC 155 0.0 28.349997 2 CTTTATA 55 4.947482E-6 27.963406 2 CGAAACT 95 9.495125E-10 25.44039 25 CGGAGTG 70 1.1265456E-6 25.109995 32 CTATACA 80 1.2928285E-7 24.73082 1 GTAGATA 45 0.0013733876 24.49074 38 >>END_MODULE