FastQCFastQC Report
Wed 25 May 2016
SRR1780715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93994
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5434557.8175202672511No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT90459.622954656680212RNA PCR Primer, Index 19 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG81528.672894014511565No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC58516.2248654169415065No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC15691.6692554843926208RNA PCR Primer, Index 19 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT12651.345830584930953RNA PCR Primer, Index 19 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC9991.0628337979019937No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC5200.5532268017107476No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4380.465987190671745No Hit
ATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTTCTGCTT2920.3106581271144967RNA PCR Primer, Index 19 (100% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC2560.2723578100729834No Hit
ACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTTCT2470.26278273081260506RNA PCR Primer, Index 19 (96% over 25bp)
CTTAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC1520.16171244973083387RNA PCR Primer, Index 19 (95% over 21bp)
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1440.15320126816605315No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC1310.13937059812328445No Hit
CATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTTCTGCT1300.1383067004276869RNA PCR Primer, Index 19 (96% over 28bp)
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1280.1361789050364917No Hit
TCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1160.1234121326893206No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1070.11383705342894226No Hit
CTTATCACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC980.10426197416856396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTA302.4680376E-644.0896031
CTTATCA207.741548E-444.08961
CTTAACA207.741548E-444.08961
TTATCAC207.8233075E-443.9957432
ATGAAAG207.8233075E-443.99574331
TAACACA207.8233075E-443.9957433
TATCACA207.8233075E-443.9957433
ATCACAT207.8233075E-443.9957434
AGTCTCG207.8233075E-443.99574336
TCACATC207.8233075E-443.9957435
TTAACAC207.8233075E-443.9957432
GAAAGTC207.8233075E-443.99574333
TGAAAGT207.8233075E-443.99574332
AAGTCTC207.8233075E-443.99574335
CTTTACA1600.042.71181
TTTACAC1600.042.6208762
TCTTTAT700.040.9403461
CTATACA1350.040.8237041
CCGTCTT12300.039.54567744
CTTTATA750.038.1296422
Produced by FastQC (version 0.11.5)