FastQCFastQC Report
Wed 25 May 2016
SRR1780714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89245
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAT150.002219221669.9915924
CCGTGCG150.002219221669.991599
CGGGGTG150.002219221669.9915959
CGAGAAT150.002219221669.9915914
CGAAGTT150.002219221669.9915933
TTTAGCG200.00693443252.49369454
TGTCAAA200.00693443252.49369460
ACTGATA200.00693443252.49369455
CGTGCGA200.00693443252.49369410
GGCACGG200.00693443252.49369410
CGTGAGT200.00693443252.49369416
CTGAGAC200.00693443252.49369450
GAATCCT200.00693443252.49369446
ATAGACC200.00693443252.49369436
GTAATGG200.00693443252.49369458
TGGCACG200.00693443252.4936949
CCCGTGC200.00693443252.4936948
GAGCACT200.00693443252.49369461
TGAATCC200.00693443252.49369445
CGCAAGT200.00693443252.49369451
Produced by FastQC (version 0.11.5)