##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780714_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 89245 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.051330606756682 34.0 31.0 34.0 12.0 34.0 2 31.450882402375484 34.0 33.0 34.0 23.0 34.0 3 31.654748165163316 34.0 34.0 34.0 23.0 34.0 4 31.969947896240686 34.0 34.0 34.0 27.0 34.0 5 31.9094851252171 34.0 34.0 34.0 27.0 34.0 6 34.95232225895008 38.0 36.0 38.0 27.0 38.0 7 34.10647095075355 38.0 34.0 38.0 23.0 38.0 8 33.19954059050927 37.0 34.0 38.0 21.0 38.0 9 33.74327973555941 37.0 34.0 38.0 21.0 38.0 10-11 34.22526752199003 37.5 34.5 38.0 24.0 38.0 12-13 34.57918650904813 38.0 35.5 38.0 25.0 38.0 14-15 34.15868115860833 38.0 34.0 38.0 23.5 38.0 16-17 34.02213009132164 38.0 34.0 38.0 23.0 38.0 18-19 34.018393187293405 37.5 34.0 38.0 22.5 38.0 20-21 34.097422824808106 38.0 34.0 38.0 23.0 38.0 22-23 34.073141352456716 37.5 34.0 38.0 23.0 38.0 24-25 34.47490055465292 38.0 35.5 38.0 25.0 38.0 26-27 34.54012549722673 38.0 35.5 38.0 25.0 38.0 28-29 34.70368087848058 38.0 36.0 38.0 26.0 38.0 30-31 34.54726875455208 38.0 36.0 38.0 25.0 38.0 32-33 34.252227015519075 38.0 34.5 38.0 24.0 38.0 34-35 34.14273628774721 37.0 34.0 38.0 23.5 38.0 36-37 34.12142416942126 37.5 34.0 38.0 23.5 38.0 38-39 34.666194184548154 38.0 36.0 38.0 26.0 38.0 40-41 34.181791697013836 38.0 34.5 38.0 23.5 38.0 42-43 34.516874894952096 38.0 35.5 38.0 25.0 38.0 44-45 33.518236315759985 37.0 34.0 38.0 22.0 38.0 46-47 34.28777522550283 37.0 34.5 38.0 24.0 38.0 48-49 34.03142472967673 37.0 34.0 38.0 23.5 38.0 50-51 33.981561992268475 37.0 34.0 38.0 23.0 38.0 52-53 33.99108633536893 37.0 34.0 38.0 23.0 38.0 54-55 33.702246624460756 37.0 34.0 38.0 22.5 38.0 56-57 33.81275701719984 37.0 34.0 38.0 23.0 38.0 58-59 33.66045156591406 37.0 33.5 38.0 22.5 38.0 60-61 33.714583450053226 37.0 34.0 38.0 22.5 38.0 62-63 33.802409098548935 37.0 34.0 38.0 23.0 38.0 64-65 33.82830970922741 37.0 34.0 38.0 23.0 38.0 66-67 33.7364838366295 37.0 34.0 38.0 22.5 38.0 68-69 33.289226287186956 37.0 32.5 38.0 21.0 38.0 70-71 33.10783237156143 37.0 32.5 38.0 20.5 38.0 72-73 33.21458905260799 37.0 33.0 38.0 20.5 38.0 74-75 32.7830130539526 37.0 31.5 38.0 19.5 38.0 76 32.013065157711914 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.979198039059181 1101 2 -1.1014968855256129 1101 3 -0.818586828083653 1101 4 -3.341234008473375 1101 5 -1.2089666967887247 1101 6 -2.8630777077168403 1101 7 -1.4477201496469903 1101 8 -1.513586105557902 1101 9 -2.4494813452853954 1101 10-11 -1.7083052402492243 1101 12-13 -2.50547587748661 1101 14-15 -0.9914190607797693 1101 16-17 -2.3042176650294905 1101 18-19 -0.33289754818207484 1101 20-21 -1.310338936052787 1101 22-23 -0.9238269981985852 1101 24-25 -1.9114101640463588 1101 26-27 -2.974223599250017 1101 28-29 -1.1713737432060611 1101 30-31 -2.0953874981698064 1101 32-33 -0.7459950021175104 1101 34-35 -1.292633135392414 1101 36-37 -0.8731969065267791 1101 38-39 -1.4643974405136078 1101 40-41 -0.8013105268612222 1101 42-43 -1.5342518847409394 1101 44-45 -0.8918467393794174 1101 46-47 -0.6953977126819808 1101 48-49 -1.6619793672943786 1101 50-51 -1.382748566578428 1101 52-53 -0.5092109547035548 1101 54-55 -2.311830699125263 1101 56-57 -1.9602306480585696 1101 58-59 -1.3069700685428884 1101 60-61 -0.7964208435893383 1101 62-63 -2.735466847684222 1101 64-65 -1.744808836556743 1101 66-67 -2.0675058302149267 1101 68-69 -0.2944803862013359 1101 70-71 0.00502654231298294 1101 72-73 -0.8440178199123451 1101 74-75 -1.9324352783512992 1101 76 -2.2707953665258422 1102 1 0.8905337617071005 1102 2 -0.44632447173250966 1102 3 0.3183863519929737 1102 4 0.38865104899788605 1102 5 0.44540750499927384 1102 6 1.4204471964977188 1102 7 1.4770882794717863 1102 8 2.1701622980180844 1102 9 1.1696118858767974 1102 10-11 -0.13774329899762705 1102 12-13 -3.9291668097982324 1102 14-15 -0.8404771706137453 1102 16-17 -2.777557384058859 1102 18-19 -0.7447749428180899 1102 20-21 0.008945865990639845 1102 22-23 -0.49438893945018236 1102 24-25 0.39087272228825753 1102 26-27 -6.321445821472238 1102 28-29 -0.09179200629673545 1102 30-31 0.5782236129413079 1102 32-33 -6.861416458056212 1102 34-35 -0.2352416922251095 1102 36-37 0.23687652897768885 1102 38-39 0.5083037089116758 1102 40-41 0.4054263824618971 1102 42-43 0.4963196350547179 1102 44-45 -6.668347377949022 1102 46-47 -0.2779328340102083 1102 48-49 0.31367516654980676 1102 50-51 -6.587569767089281 1102 52-53 -1.8425442880368834 1102 54-55 0.6445071041952986 1102 56-57 -1.1593845433331538 1102 58-59 0.7087547079577519 1102 60-61 0.3341666404464192 1102 62-63 -4.802915762115894 1102 64-65 -1.03160640999225 1102 66-67 -0.2729656003298615 1102 68-69 -7.656788815320116 1102 70-71 -0.6044881447879149 1102 72-73 -0.23897312004006466 1102 74-75 -7.224501051020518 1102 76 -2.8352908965386163 1103 1 -0.7584715433326856 1103 2 -0.6611122701409968 1103 3 -0.43055264004946636 1103 4 -0.16943913667850552 1103 5 -0.26957923240125936 1103 6 -1.844274288913418 1103 7 -0.09857485050169146 1103 8 1.636342669370869 1103 9 0.18698589118184117 1103 10-11 0.017727523283546986 1103 12-13 -1.6278405498512853 1103 14-15 -0.53208857644929 1103 16-17 -0.4599512832631092 1103 18-19 -0.8608890011735255 1103 20-21 -1.1182093064231537 1103 22-23 -0.6767828386544323 1103 24-25 0.04140322626702897 1103 26-27 -0.8887535137799318 1103 28-29 0.14169605736374535 1103 30-31 -0.130430233212536 1103 32-33 -6.195567651690162 1103 34-35 -1.251322594081877 1103 36-37 -0.3939590147888836 1103 38-39 0.08207691846074994 1103 40-41 0.04733619678549417 1103 42-43 0.048938998449941096 1103 44-45 -4.63187522940791 1103 46-47 -0.297926202710471 1103 48-49 0.03260752729251948 1103 50-51 -0.2911175124473715 1103 52-53 -0.9454289034215009 1103 54-55 -0.3069517817463421 1103 56-57 0.2429032550753334 1103 58-59 0.01575072917790976 1103 60-61 0.1384438287753369 1103 62-63 -0.029341491558867006 1103 64-65 0.41633788708998054 1103 66-67 0.04467365696456227 1103 68-69 -3.4859333776543338 1103 70-71 -0.08485949757305633 1103 72-73 0.8127841743896482 1103 74-75 -1.352485135901155 1103 76 0.23825021751974162 1104 1 -1.3317966053674262 1104 2 0.16059988866793518 1104 3 0.9013952507693936 1104 4 0.3832283571180213 1104 5 0.3016366484920425 1104 6 0.39722695178137 1104 7 1.4035343306397507 1104 8 2.1608463914552374 1104 9 0.25720921624387216 1104 10-11 0.26242355306021636 1104 12-13 -0.5058492347148089 1104 14-15 0.14381092727280276 1104 16-17 -0.2079661716005745 1104 18-19 0.9892350683053834 1104 20-21 1.6559842419639423 1104 22-23 0.8514119504274547 1104 24-25 0.9101997523216241 1104 26-27 1.0898445011084021 1104 28-29 0.19324692585009728 1104 30-31 -0.4681473548006281 1104 32-33 -0.345009339035073 1104 34-35 0.7482121453722215 1104 36-37 0.6150050050622298 1104 38-39 0.2002530971208003 1104 40-41 1.5875036905820252 1104 42-43 1.0793980555219065 1104 44-45 -2.697476966380613 1104 46-47 0.6038107699942472 1104 48-49 1.2400516960270025 1104 50-51 1.5404891873044875 1104 52-53 0.33084280873730876 1104 54-55 0.15688196515191777 1104 56-57 0.9615136769115651 1104 58-59 1.1821279003937875 1104 60-61 0.10516219105821278 1104 62-63 0.47099969950813403 1104 64-65 0.44642473572521624 1104 66-67 0.6549404063442168 1104 68-69 -2.9380735761655004 1104 70-71 -0.2522912593596587 1104 72-73 0.975501295977736 1104 74-75 -0.7208164014098415 1104 76 0.3786968676438107 1105 1 -1.0357225868628 1105 2 -0.6237837072310377 1105 3 -0.7772431588330058 1105 4 -0.7210789697136875 1105 5 -0.5863353445062067 1105 6 -0.6996410152103749 1105 7 0.9179129252884124 1105 8 0.019467296681547452 1105 9 0.28415345230288835 1105 10-11 0.22714910944931432 1105 12-13 -2.356358737090403 1105 14-15 -1.0869186301165499 1105 16-17 0.30425563385078647 1105 18-19 0.2220120125414411 1105 20-21 -0.6317751978960189 1105 22-23 -7.542161141813608E-4 1105 24-25 -0.11527536645807146 1105 26-27 0.00994952736496657 1105 28-29 -1.0719443719743538 1105 30-31 -0.3011368521749702 1105 32-33 -0.05865650082552065 1105 34-35 0.204029224642035 1105 36-37 0.5302806989857061 1105 38-39 -0.16255635524229461 1105 40-41 -0.06379976028068768 1105 42-43 0.4892192608232335 1105 44-45 1.6405606680279945 1105 46-47 0.5467965164308524 1105 48-49 -0.1276339753908431 1105 50-51 -0.4137024856137401 1105 52-53 -1.0862458384244817 1105 54-55 -0.7339344889616086 1105 56-57 -0.34578189698191153 1105 58-59 -0.027361972074324115 1105 60-61 -0.25900697623361424 1105 62-63 0.0023669163123329895 1105 64-65 -0.1356895428444247 1105 66-67 0.11078874342848621 1105 68-69 1.0162431280105437 1105 70-71 0.4907069902027317 1105 72-73 -0.3630208345548951 1105 74-75 -1.2480575867061674 1105 76 -0.16625186954048488 1106 1 -0.9013838711795117 1106 2 -0.23632221303653012 1106 3 -0.9170827038721043 1106 4 -0.17820635199593937 1106 5 0.5504745129799957 1106 6 -0.3556783869987399 1106 7 1.17188198523899 1106 8 1.8486369253332526 1106 9 1.754990593902697 1106 10-11 0.8134782790779695 1106 12-13 1.2789532022481467 1106 14-15 0.5573638924791666 1106 16-17 1.3896708195249374 1106 18-19 0.4601640723904623 1106 20-21 0.543362745978591 1106 22-23 0.5642148099851383 1106 24-25 1.1404962729583374 1106 26-27 0.9351043968683683 1106 28-29 -0.27248768465842943 1106 30-31 -0.19006241325956097 1106 32-33 1.3568676858980098 1106 34-35 0.7692098211011924 1106 36-37 1.1847947641832377 1106 38-39 0.9749220645810013 1106 40-41 0.6677174530837888 1106 42-43 1.223309169763354 1106 44-45 2.1791341760338128 1106 46-47 1.1236727223819045 1106 48-49 1.3598749105642653 1106 50-51 1.4481082181539051 1106 52-53 0.44157143248713027 1106 54-55 0.9594069686735409 1106 56-57 1.4212791368354942 1106 58-59 0.7683372256508676 1106 60-61 1.8211697273309326 1106 62-63 1.534204024060891 1106 64-65 1.8309331171873922 1106 66-67 1.6391899485332573 1106 68-69 1.410153286772946 1106 70-71 1.2969762431054832 1106 72-73 1.438136878454145 1106 74-75 -0.8978747899152637 1106 76 -0.3211552242001119 1107 1 -0.45285704289059936 1107 2 0.46220043248204945 1107 3 0.6603403749814838 1107 4 0.17983878846148826 1107 5 0.11111631725980686 1107 6 0.20876137274293427 1107 7 0.12699249403117108 1107 8 0.4565607654510373 1107 9 -1.3035681907515553 1107 10-11 -0.619304601679616 1107 12-13 0.3586684392439068 1107 14-15 -0.3067128027976551 1107 16-17 0.6221073668989945 1107 18-19 0.6785967046914934 1107 20-21 0.9469247931937019 1107 22-23 -0.184283575465507 1107 24-25 0.30753938895492183 1107 26-27 0.48219402527105615 1107 28-29 -1.1301061825419438 1107 30-31 0.30068529876506034 1107 32-33 1.512625687537657 1107 34-35 0.04564911237259395 1107 36-37 -0.35459856680775204 1107 38-39 0.9932175020151206 1107 40-41 0.9451773403162989 1107 42-43 0.15221043965242131 1107 44-45 0.07351085959886916 1107 46-47 0.4818851736526284 1107 48-49 0.3713858945191575 1107 50-51 0.9054857884662724 1107 52-53 1.1730208460627338 1107 54-55 0.660790629099516 1107 56-57 1.273326184636197 1107 58-59 1.3962547079577519 1107 60-61 -0.04155175035817393 1107 62-63 0.2547949658534563 1107 64-65 0.5110181110805456 1107 66-67 1.170042062505381 1107 68-69 1.3348299586262442 1107 70-71 -0.2974958076231573 1107 72-73 -0.13384859896726198 1107 74-75 -0.30831331155691544 1107 76 -1.0541606283393818 1108 1 -0.5294853953810197 1108 2 0.21698970451270227 1108 3 0.2503786891577242 1108 4 0.015087830607086516 1108 5 0.42912398009505637 1108 6 0.6800257405590244 1108 7 1.0848469001614376 1108 8 1.2228442903552548 1108 9 0.7883858322369548 1108 10-11 0.6393160879755513 1108 12-13 0.6326147993971674 1108 14-15 1.2066971588881685 1108 16-17 -0.11544052582132025 1108 18-19 0.28779210699033797 1108 20-21 0.4852389694389103 1108 22-23 -0.23600771339654614 1108 24-25 -0.4836483505086804 1108 26-27 -0.10975999771744682 1108 28-29 0.39671374082970345 1108 30-31 0.7719496665811576 1108 32-33 0.935997335047233 1108 34-35 0.5858789974300649 1108 36-37 0.6339071803186869 1108 38-39 1.2786581150419423 1108 40-41 0.692303777097905 1108 42-43 0.9319039262424553 1108 44-45 0.9336641163038522 1108 46-47 0.9703909207790673 1108 48-49 0.44035141176053827 1108 50-51 0.811616056665514 1108 52-53 0.2304921104305535 1108 54-55 0.664622046724034 1108 56-57 1.1660464911496007 1108 58-59 -0.6707951004713664 1108 60-61 1.270708786040295 1108 62-63 0.5670555022519181 1108 64-65 0.7121675363679074 1108 66-67 0.8194673498617036 1108 68-69 1.6777418360208785 1108 70-71 1.8461823532963777 1108 72-73 0.814427263101706 1108 74-75 1.4962843895925033 1108 76 1.7006286436912674 1109 1 0.5557852525030569 1109 2 -0.11935402838275522 1109 3 0.9654566138545242 1109 4 0.30037000155168414 1109 5 0.7423351689868269 1109 6 1.279988290612316 1109 7 -0.003693416146575146 1109 8 1.1606341673465792 1109 9 1.276430656942395 1109 10-11 1.3670045620342677 1109 12-13 1.0690507167768217 1109 14-15 1.1988830834697453 1109 16-17 -0.1422676511057901 1109 18-19 0.9486396280919465 1109 20-21 1.4325425732760948 1109 22-23 1.40499853786379 1109 24-25 1.8130920637453443 1109 26-27 1.0375767349187441 1109 28-29 0.5734918931069473 1109 30-31 1.1087687257232588 1109 32-33 1.4774803529367944 1109 34-35 0.2883679785034374 1109 36-37 1.0313735473857832 1109 38-39 1.2920248652508022 1109 40-41 1.0267318011811 1109 42-43 1.0272212425216622 1109 44-45 0.3893075351959041 1109 46-47 0.6407377649008694 1109 48-49 0.46879896743131866 1109 50-51 1.4941485372923609 1109 52-53 1.456797817226274 1109 54-55 1.0614114339852918 1109 56-57 0.9163705772268713 1109 58-59 0.9363117471073537 1109 60-61 1.225387148613386 1109 62-63 0.31636411862042735 1109 64-65 0.5150943937412222 1109 66-67 0.8504139692838208 1109 68-69 1.9137485193959876 1109 70-71 1.001650045515433 1109 72-73 0.3243370951530977 1109 74-75 1.9714737998998828 1109 76 0.14834275655965 1110 1 0.35117536713389796 1110 2 0.06038836037602735 1110 3 -0.5763281759889374 1110 4 -0.3152777243203708 1110 5 0.00589365536671238 1110 6 0.1742629115909935 1110 7 1.1064692856899825 1110 8 2.094413287277554 1110 9 -1.5197606409745248 1110 10-11 -1.3460177478387791 1110 12-13 -0.07522390420184877 1110 14-15 -0.04917558779859377 1110 16-17 0.28932362215933694 1110 18-19 0.5166015410711182 1110 20-21 0.4043316771834071 1110 22-23 0.4249510831614529 1110 24-25 0.5152367697727058 1110 26-27 -1.2271835263902702 1110 28-29 0.3734425895201099 1110 30-31 0.1571170555642567 1110 32-33 1.1469922474271073 1110 34-35 0.7132108233203951 1110 36-37 0.01436380992738151 1110 38-39 0.4553429966448661 1110 40-41 0.521169740291171 1110 42-43 1.0478671195092133 1110 44-45 1.7669267882465718 1110 46-47 0.5484167985505621 1110 48-49 1.0849089144299668 1110 50-51 0.2682456653584353 1110 52-53 0.08266062999590673 1110 54-55 -0.04585468212465571 1110 56-57 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2116 50-51 -0.291758538088402 2116 52-53 1.0020069940144012 2116 54-55 0.22039864560407807 2116 56-57 0.15123658840866483 2116 58-59 -0.753907390480208 2116 60-61 -1.0220262566947511 2116 62-63 0.9828379956206206 2116 64-65 0.685568656320747 2116 66-67 -0.7427195054286031 2116 68-69 -0.053027394748347945 2116 70-71 -3.514560352273911 2116 72-73 0.6219722085776809 2116 74-75 -1.5180834264994445 2116 76 -1.8160232867537651 2117 1 -0.14419803905918016 2117 2 -0.3164968855256127 2117 3 0.039190949694123844 2117 4 -0.3856784529178192 2117 5 -0.9524852153072416 2117 6 -0.5264110410501672 2117 7 -2.5943868163136585 2117 8 -2.0867342537060516 2117 9 0.5345927287886809 2117 10-11 -0.33876820321218304 2117 12-13 0.4243389373282085 2117 14-15 -0.8379005422612522 2117 16-17 -0.7226435909554212 2117 18-19 -0.8684531037376289 2117 20-21 -0.938209306423154 2117 22-23 -0.2166973685689655 2117 24-25 7.194655832734043E-4 2117 26-27 0.6774430674166467 2117 28-29 0.007978108645801285 2117 30-31 -0.2137208315031316 2117 32-33 0.5362272201047062 2117 34-35 -0.35948498724426514 2117 36-37 -1.5653265361564053 2117 38-39 0.15643589281972226 2117 40-41 -1.4539031194538126 2117 42-43 -2.2616592921483516 2117 44-45 -0.052772665305347743 2117 46-47 -0.5716014163856826 2117 48-49 -0.6729052932203032 2117 50-51 0.1890106926808386 2117 52-53 1.0010668230742326 2117 54-55 -1.2217381065326691 2117 56-57 -1.4700454628733866 2117 58-59 -0.6504885870614032 2117 60-61 0.8595976749291836 2117 62-63 -1.3875038847212622 2117 64-65 -1.094345873593781 2117 66-67 -1.6608391635482604 2117 68-69 -0.957813719534677 2117 70-71 0.48817469046112905 2117 72-73 0.5527414393469101 2117 74-75 -0.46808342649944734 2117 76 -1.0720916628221389 2118 1 0.8813900594996298 2118 2 0.674130729537147 2118 3 0.639804617755491 2118 4 0.3435637600064041 2118 5 0.08359102830038267 2118 6 0.5377823573691671 2118 7 0.3058828257133044 2118 8 -3.9561903206516575 2118 9 -0.9522320038937977 2118 10-11 -0.11376587871195198 2118 12-13 0.6982859387229041 2118 14-15 -0.49143843161504464 2118 16-17 -0.16189045288009396 2118 18-19 -0.5844084733331627 2118 20-21 -2.8023097248331936 2118 22-23 -0.10100234299945754 2118 24-25 -0.1330415757928307 2118 26-27 0.6189307475654147 2118 28-29 -0.17316554546857077 2118 30-31 0.018338675702814555 2118 32-33 0.46297756877974194 2118 34-35 1.2457404892782833 2118 36-37 -0.1773674473604956 2118 38-39 0.029936590169789667 2118 40-41 -1.4508533751488386 2118 42-43 0.07182280919986539 2118 44-45 0.1475341687253362 2118 46-47 -0.461717641397307 2118 48-49 -0.7659038985201647 2118 50-51 0.2671139004911538 2118 52-53 -1.3877848738109435 2118 54-55 -0.270115605370421 2118 56-57 1.1916560712967694 2118 58-59 1.1599251739799712 2118 60-61 -1.267180567748646 2118 62-63 0.39596426962555853 2118 64-65 -0.2256801367272132 2118 66-67 -1.830406806505021 2118 68-69 1.170535885300282 2118 70-71 0.17020165466382764 2118 72-73 -1.3964449855717334 2118 74-75 0.26422712673161186 2118 76 -0.11445335505830911 2119 1 0.9478221629610211 2119 2 0.5735031144743843 2119 3 0.6016151921183699 2119 4 0.31482659758722775 2119 5 0.5283228655008401 2119 6 0.6753061306670034 2119 7 -0.8436797456065861 2119 8 1.3905384735666786 2119 9 0.4341886883846371 2119 10-11 0.4341610897171151 2119 12-13 0.651005603994868 2119 14-15 0.13871561935490462 2119 16-17 -0.12900722731905034 2119 18-19 -0.7687561340406646 2119 20-21 -0.7341183973322458 2119 22-23 0.8041612172896251 2119 24-25 0.6415780514418614 2119 26-27 0.7035541785277601 2119 28-29 1.0899478056154948 2119 30-31 0.013450885668582657 2119 32-33 0.7156211594986459 2119 34-35 0.7605655178062349 2119 36-37 -0.3777002735301451 2119 38-39 -0.11477622839239388 2119 40-41 0.41114738559669206 2119 42-43 -0.00630575679480927 2119 44-45 -0.9397423622750409 2119 46-47 -0.18079333557760435 2119 48-49 -0.7303295356445503 2119 50-51 1.1405258441959845 2119 52-53 -0.9459533789459726 2119 54-55 -0.7011825509771157 2119 56-57 1.1969242340963149 2119 58-59 1.518198281625459 2119 60-61 0.6800522203837218 2119 62-63 0.22572843851106228 2119 64-65 0.652876348628439 2119 66-67 0.727191139482045 2119 68-69 -0.5962480629690177 2119 70-71 -0.8602091479227099 2119 72-73 -1.4060969444914733 2119 74-75 0.2854519270359077 2119 76 0.9417467210162442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 16.0 13 84.0 14 241.0 15 432.0 16 642.0 17 726.0 18 771.0 19 737.0 20 726.0 21 703.0 22 689.0 23 653.0 24 783.0 25 881.0 26 1081.0 27 1323.0 28 1636.0 29 1945.0 30 2373.0 31 3035.0 32 3884.0 33 5113.0 34 7268.0 35 10798.0 36 18467.0 37 24237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.71906052393857 21.4916440831075 12.163504968383016 28.625790424570912 2 19.16858087287803 19.372513866323043 35.365566698414476 26.09333856238445 3 17.529273348646985 25.060227463723457 28.36237324219844 29.04812594543112 4 13.185052383887053 17.493416998151158 36.41884699422937 32.90268362373242 5 13.98957924813715 36.09277830690795 34.37279399406129 15.544848450893609 6 28.226791416886098 37.99764692699871 17.097876631744075 16.677685024371115 7 27.547761779371392 30.895848506919155 21.347974676452463 20.208415037256987 8 23.442209647599306 35.43503837750014 19.113675836181297 22.009076138719255 9 22.955907893999665 17.42058378620651 21.561992268474427 38.0615160513194 10-11 24.84284833884251 25.699478962406857 26.63230433077483 22.825368367975795 12-13 23.61476833436047 23.881449941173173 26.580200571460587 25.92358115300577 14-15 21.299792705473696 25.79192111602891 23.79629110874559 29.111995069751806 16-17 21.902067342708275 27.991484116757242 26.139279511457225 23.967169029077258 18-19 21.20623004089865 27.55056305675388 26.907389769735 24.335817132612473 20-21 21.101580489553978 27.938416372997775 26.42739888732639 24.532604250121857 22-23 22.343548658188134 27.112443274133003 27.084430500308144 23.459577567370722 24-25 21.487478290100288 28.390946271499807 26.552748053112218 23.56882738528769 26-27 20.739537228976413 28.53213065157712 27.05977925934226 23.668552860104207 28-29 21.63482548041907 27.157823967729282 28.294582329542273 22.912768222309374 30-31 21.425289932209086 27.430668384783463 27.57969634153174 23.56434534147571 32-33 20.826376827833492 28.271051599529386 26.96285506190823 23.939716510728893 34-35 21.98778643061236 27.399854333576112 27.423945319065496 23.188413916746036 36-37 22.192839935010365 26.968457616673202 27.152781668440806 23.685920779875623 38-39 21.28298504117878 27.601546305115132 27.697349991596166 23.418118662109922 40-41 21.405120735055185 26.403159840887447 27.914168861000615 24.277550563056753 42-43 21.02694828841952 25.798083926270383 28.474984592974394 24.699983192335708 44-45 20.742338506358898 25.967841335649055 28.88509160177041 24.404728556221635 46-47 20.694156535380134 25.173959325452405 29.322651128914785 24.809233010252676 48-49 19.806711860608438 26.11462827049134 29.79830802846098 24.28035184043924 50-51 19.126561712140735 26.362821446579645 29.49745083758194 25.013166003697684 52-53 19.087904084262426 26.360580424673653 29.534427699030758 25.017087792033166 54-55 18.466580760826936 26.391394475881004 29.390442041570957 25.751582721721107 56-57 18.646422768782564 26.627822286962854 29.133284777858705 25.592470166395877 58-59 18.901339010588828 26.191943526247968 28.887892879152893 26.018824584010307 60-61 18.924309485125217 25.811530057706317 29.264944814835562 25.999215642332903 62-63 19.118718135469777 26.28270491344053 28.762395652417506 25.836181298672194 64-65 20.21065605916298 25.938708050871195 27.615552692027563 26.23508319793826 66-67 19.776458064877584 25.92021962014679 27.081629222925653 27.221693092049975 68-69 19.98991540142305 25.321866771247688 26.851924477561766 27.836293349767494 70-71 21.344052888116984 25.215978486189705 26.701776009860495 26.73819261583282 72-73 21.460586027228416 25.205893887612753 26.1443218107457 27.189198274413133 74-75 22.17883354809793 26.00649896352737 25.87651969298 25.938147795394702 76 22.70715446243487 24.775617681662837 26.160569219564124 26.35665863633817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 3.5 16 6.5 17 8.0 18 11.0 19 18.0 20 25.0 21 28.0 22 42.0 23 60.5 24 88.5 25 112.0 26 159.5 27 287.5 28 421.0 29 474.0 30 532.0 31 762.0 32 1147.5 33 1361.0 34 1514.0 35 1992.5 36 2415.5 37 2513.0 38 2864.0 39 3704.0 40 4526.0 41 5136.0 42 5413.0 43 5600.0 44 6183.0 45 6562.0 46 6545.0 47 6521.0 48 6254.0 49 5856.5 50 5702.0 51 5352.0 52 4527.5 53 3753.5 54 3454.0 55 3216.5 56 2611.0 57 1966.5 58 1690.0 59 1416.5 60 1031.5 61 770.0 62 620.0 63 537.5 64 423.0 65 313.5 66 194.5 67 153.0 68 134.5 69 97.5 70 66.5 71 54.0 72 41.0 73 23.0 74 16.0 75 14.0 76 9.5 77 4.0 78 5.0 79 7.0 80 5.5 81 5.0 82 4.0 83 2.0 84 1.5 85 2.5 86 3.0 87 2.0 88 1.0 89 1.5 90 3.0 91 3.0 92 3.0 93 2.0 94 0.5 95 0.5 96 1.0 97 1.5 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7675500028012774 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.602554764972828E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 89245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.239957420583785 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99772698573052 38.33155919099109 2 11.114618849181294 11.8348366855286 3 6.05084817106537 9.664406969578128 4 3.489497832217873 7.431228640259959 5 2.0730731994780487 5.5185164434982354 6 1.2796228480026939 4.087623956524175 7 0.8586942795807552 3.200179281752479 8 0.7345203518962832 3.128466580760827 9 0.515637496316875 2.470726651353017 >10 1.8794460580039567 14.153173847274358 >50 0.006313928526329082 0.17928175247913047 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005602554764972827 0.0 2 0.0 0.0 0.0 0.01008459857695109 0.0 3 0.0 0.0 0.0 0.01008459857695109 0.0 4 0.0 0.0 0.0 0.01568715334192392 0.0 5 0.0 0.0 0.0 0.016807664294918482 0.0 6 0.0 0.0 0.0 0.016807664294918482 0.0 7 0.0 0.0 0.0 0.026892262871869573 0.0 8 0.0 0.0 0.0 0.028012773824864137 0.0 9 0.0 0.0 0.0 0.039217883354809795 0.0 10 0.0 0.0 0.0 0.05490503669673371 0.0 11 0.0 0.0 0.0 0.07059219003865763 0.0 12 0.0 0.0 0.0 0.07731525575662503 0.0 13 0.0 0.0 0.0 0.08403832147459242 0.0 14 0.0 0.0 0.0 0.08964087623956524 0.0 15 0.0 0.0 0.0 0.09300240909854894 0.0 16 0.0 0.0 0.0 0.0941229200515435 0.0 17 0.0 0.0 0.0 0.1008459857695109 0.0 18 0.0 0.0 0.0 0.11093058434646198 0.0 19 0.0 0.0 0.0 0.11653313911143481 0.0 20 0.0 0.0 0.0 0.11877416101742394 0.0 21 0.0 0.0 0.0 0.12101518292341308 0.0 22 0.0 0.0 0.0 0.1400638691243207 0.0 23 0.0 0.0 0.0 0.14230489103030983 0.0 24 0.0 0.0 0.0 0.14678693484228808 0.0 25 0.0 0.0 0.0 0.15126897865426633 0.0 26 0.0 0.0 0.0 0.15463051151325005 0.0 27 0.0 0.0 0.0 0.18488430724410332 0.0 28 0.0 0.0 0.0 0.21625861392795115 0.0 29 0.0 0.0 0.0 0.25547649728276095 0.0 30 0.0 0.0 0.0 0.299176424449549 0.0 31 0.0 0.0 0.0 0.32382766541542946 0.0 32 0.0 0.0 0.0 0.3708891254412012 0.0 33 0.0 0.0 0.0 0.40786598689002185 0.0 34 0.0 0.0 0.0 0.4818197097876632 0.0 35 0.0 0.0 0.0 0.5871477393691523 0.0 36 0.0 0.0 0.0 0.7922012437671578 0.0 37 0.0 0.0 0.0 1.1709339458793209 0.0 38 0.0 0.0 0.0 1.6224998599361309 0.0 39 0.0 0.0 0.0 2.199563000728332 0.0 40 0.0 0.0 0.0 2.893159280631968 0.0 41 0.0 0.0 0.0 3.8489551235363324 0.0 42 0.0 0.0 0.0 5.000840383214746 0.0 43 0.0 0.0 0.0 6.2390049862737404 0.0 44 0.0 0.0 0.0 7.2463443330158555 0.0 45 0.0 0.0 0.0 8.287299008347807 0.0 46 0.0 0.0 0.0 9.404448428483388 0.0 47 0.0 0.0 0.0 10.363605804246736 0.0 48 0.0 0.0 0.0 11.27009916521934 0.0 49 0.0 0.0 0.0 12.154182307132052 0.0 50 0.0 0.0 0.0 13.002409098548938 0.0 51 0.0 0.0 0.0 13.988458737184155 0.0 52 0.0 0.0 0.0 15.036136478234075 0.0 53 0.0 0.0 0.0 16.167852540758584 0.0 54 0.0 0.0 0.0 17.262591741834278 0.0 55 0.0 0.0 0.0 18.223990139503613 0.0 56 0.0 0.0 0.0 19.025155470894727 0.0 57 0.0 0.0 0.0 19.87898481707659 0.0 58 0.0 0.0 0.0 20.57146058602723 0.0 59 0.0 0.0 0.0 21.26393635497787 0.0 60 0.0 0.0 0.0 21.996750518236315 0.0 61 0.0 0.0 0.0 22.708274973387866 0.0 62 0.0 0.0 0.0 23.541935122415822 0.0 63 0.0 0.0 0.0 24.317328701888062 0.0 64 0.0 0.0 0.0 25.07815563897137 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATGTAT 15 0.0022192216 69.99159 24 CCGTGCG 15 0.0022192216 69.99159 9 CGGGGTG 15 0.0022192216 69.99159 59 CGAGAAT 15 0.0022192216 69.99159 14 CGAAGTT 15 0.0022192216 69.99159 33 TTTAGCG 20 0.006934432 52.493694 54 TGTCAAA 20 0.006934432 52.493694 60 ACTGATA 20 0.006934432 52.493694 55 CGTGCGA 20 0.006934432 52.493694 10 GGCACGG 20 0.006934432 52.493694 10 CGTGAGT 20 0.006934432 52.493694 16 CTGAGAC 20 0.006934432 52.493694 50 GAATCCT 20 0.006934432 52.493694 46 ATAGACC 20 0.006934432 52.493694 36 GTAATGG 20 0.006934432 52.493694 58 TGGCACG 20 0.006934432 52.493694 9 CCCGTGC 20 0.006934432 52.493694 8 GAGCACT 20 0.006934432 52.493694 61 TGAATCC 20 0.006934432 52.493694 45 CGCAAGT 20 0.006934432 52.493694 51 >>END_MODULE