FastQCFastQC Report
Wed 25 May 2016
SRR1780714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89245
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT11461.2841055521317721No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5180.580424673651185RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1280.1434254019833044No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1080.12101518292341308No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1040.11653313911143481No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAA150.002218164370.04
GGCGATC150.002218164370.05
CAATCGC150.002218164370.048
TCAACCG150.002218164370.032
GACATCG252.3790592E-455.99999622
ACATCGT252.3790592E-455.99999623
CTTGTTA200.006931137352.50000447
CAGGTTG200.006931137352.50000425
TGTCATG200.006931137352.50000425
CTTCGGA200.006931137352.50000466
CGGAAGC200.006931137352.5000046
TGGCGAT200.006931137352.5000044
GATCCGG200.006931137352.50000422
CAACCGC200.006931137352.50000433
GGATCCG200.006931137352.50000421
CACGATG200.006931137352.5000042
GGTCACC200.006931137352.50000431
ACGGAAG200.006931137352.5000045
CAGGAAC200.006931137352.50000419
CCCTTAG200.006931137352.50000445
Produced by FastQC (version 0.11.5)