FastQCFastQC Report
Wed 25 May 2016
SRR1780713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728113
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG207.8326423E-444.02498211
CAAATCG301.3057653E-436.64209729
CGGTGTT250.002333780535.25638215
TCGCCGT300.00570069429.37829219
CGTGCGA901.6370905E-1129.34998510
TGATACG300.00574883729.3277871
GTACAAG1050.029.3277871
CGAATAT602.9510738E-729.32375525
GATACGC300.005783698529.2915292
TATACCG1250.028.1179376
AAGCGCG554.9277114E-628.02168324
GCGCGTA554.9860682E-627.97931526
CGCGTAC554.9860682E-627.97931527
GTTAAGC555.0154704E-627.9581763
CGAATGT406.963958E-427.54404815
GGCTACC407.037192E-427.49481
GAACGGC804.3437467E-927.47968328
CGGACAG407.0911093E-427.4589235
ATACCGC1300.027.0364787
AGAACGG508.378963E-526.38049527
Produced by FastQC (version 0.11.5)