FastQCFastQC Report
Wed 25 May 2016
SRR1780713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728113
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT148232.035810375587306No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT54980.7551025733642992Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC19430.26685418334791444No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG14640.2010676914160302No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC8020.11014773805714223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT207.860738E-443.99299617
CCGTCTT7300.037.36904544
CCGGACT250.0023516435.201654
GCCGTCT8250.033.06594543
CGGTAAG801.3096724E-1030.24518628
CGTGCGA454.0603336E-529.34075410
CGGTAAC300.005736436729.34075210
CTCGAGT454.067953E-529.33269344
TATCATG300.005744145329.33269136
CGATTTG300.00574800329.32866317
ACGGATT300.00574800329.32866334
AACACCG300.00574800329.32866331
CGCAAAG554.9477476E-628.00708212
CCCAACG554.958336E-627.9993921
TCGTTCG804.311005E-927.499423
TTCGGTA804.311005E-927.499426
GTTTCCG407.0359715E-427.49562328
TGCCGTC9950.027.41648542
ATGCCGT24600.026.1132544
TATGCCG24850.025.9390743
Produced by FastQC (version 0.11.5)