##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780712_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19339 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.471741041418895 34.0 23.0 34.0 12.0 34.0 2 29.454677077408345 34.0 27.0 34.0 12.0 34.0 3 29.436992605615597 34.0 31.0 34.0 11.0 34.0 4 29.952686281607114 34.0 31.0 34.0 11.0 34.0 5 29.731682093179586 34.0 31.0 34.0 11.0 34.0 6 32.15155902580278 37.0 31.0 38.0 11.0 38.0 7 31.581726045814158 37.0 27.0 38.0 11.0 38.0 8 30.831997517968873 36.0 26.0 38.0 11.0 38.0 9 31.534567454366822 37.0 27.0 38.0 12.0 38.0 10-11 31.786002378613166 37.0 29.0 38.0 11.0 38.0 12-13 32.1154920109623 37.0 31.0 38.0 11.0 38.0 14-15 31.742023889549614 37.0 28.0 38.0 11.0 38.0 16-17 31.580536739231604 37.0 27.0 38.0 11.0 38.0 18-19 31.661357877863384 37.0 29.0 38.0 11.0 38.0 20-21 31.713687367495734 37.0 27.5 38.0 11.0 38.0 22-23 31.572444283572057 37.0 27.5 38.0 11.0 38.0 24-25 31.95860696002896 37.0 31.0 38.0 11.0 38.0 26-27 31.956461037282175 37.0 31.0 38.0 11.0 38.0 28-29 32.01988210352138 37.0 31.0 38.0 11.0 38.0 30-31 31.888618853094783 37.0 31.0 38.0 11.0 38.0 32-33 31.713583949532033 37.0 28.0 38.0 11.0 38.0 34-35 31.66952789699571 37.0 27.0 38.0 11.0 38.0 36-37 31.601737421790162 37.0 27.0 38.0 11.0 38.0 38-39 32.01582294844614 37.0 31.0 38.0 11.0 38.0 40-41 31.54661564713791 37.0 27.0 38.0 11.0 38.0 42-43 31.882930865091264 37.0 29.0 38.0 11.0 38.0 44-45 31.049356223175963 37.0 26.5 38.0 11.0 38.0 46-47 31.641268938414605 37.0 27.0 38.0 11.0 38.0 48-49 31.5182532705931 37.0 27.0 38.0 11.0 38.0 50-51 31.440095144526605 37.0 27.0 38.0 11.0 38.0 52-53 31.47055173483634 37.0 27.0 38.0 11.0 38.0 54-55 31.211567299239878 37.0 27.0 38.0 11.0 38.0 56-57 31.187393350224937 37.0 27.0 38.0 11.0 38.0 58-59 31.094860127204097 36.5 26.5 38.0 11.0 38.0 60-61 31.17679300894565 37.0 26.5 38.0 11.0 38.0 62-63 31.0790888877398 37.0 26.5 38.0 11.0 38.0 64-65 31.1582811934433 37.0 27.0 38.0 11.0 38.0 66-67 31.133538445628005 37.0 26.5 38.0 11.0 38.0 68-69 30.634184807901132 36.0 25.0 38.0 11.0 38.0 70-71 30.411887894927347 36.0 25.0 38.0 11.0 38.0 72-73 30.43182170743058 36.0 24.5 38.0 11.0 38.0 74-75 30.057241842908113 36.0 24.0 38.0 11.0 38.0 76 29.31966492579761 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.846860676574348 1101 2 2.2637592265049697 1101 3 1.3001342647043792 1101 4 -0.7103396176047241 1101 5 1.2472207064585916 1101 6 -0.5800638144465111 1101 7 1.3084707354717118 1101 8 0.6506329251845386 1101 9 2.1874970076140805 1101 10-11 1.3646819531828136 1101 12-13 1.5521070963761332 1101 14-15 1.4729724193193086 1101 16-17 0.8762674426773067 1101 18-19 2.3881415731669193 1101 20-21 1.2042684905038925 1101 22-23 1.9133349703808342 1101 24-25 1.2565304816719518 1101 26-27 0.6439864566367532 1101 28-29 0.9962325781841272 1101 30-31 0.42186264171233034 1101 32-33 2.2290360806485268 1101 34-35 0.8909758569744888 1101 36-37 1.9469796257172973 1101 38-39 1.93980805686585 1101 40-41 3.1144172048399774 1101 42-43 2.255998441447524 1101 44-45 2.3233396288625165 1101 46-47 3.2080665653694957 1101 48-49 0.3042600136604001 1101 50-51 1.8006456406884226 1101 52-53 2.7521078651202444 1101 54-55 1.951105998981408 1101 56-57 1.872049614291896 1101 58-59 0.6520738910388637 1101 60-61 1.711707954667549 1101 62-63 1.0182566817511898 1101 64-65 0.649344173545888 1101 66-67 1.1443596902487236 1101 68-69 2.5892003849141325 1101 70-71 4.00483615887234 1101 72-73 1.5962278502463931 1101 74-75 0.4667214980222596 1101 76 -0.8151397132874152 1102 1 0.3370567550057224 1102 2 -0.4016819499656208 1102 3 -0.8579539705897368 1102 4 -1.3390160881929596 1102 5 0.14795600057624014 1102 6 -0.28717165758376595 1102 7 1.6528334805697504 1102 8 1.5219564545963031 1102 9 0.06494798800623869 1102 10-11 0.3217897963200649 1102 12-13 -3.6746085898983765 1102 14-15 -0.6391599336218654 1102 16-17 -1.9466492239893647 1102 18-19 -0.32448097585269053 1102 20-21 0.6705675101117379 1102 22-23 -0.9628905198152431 1102 24-25 0.36314812873077074 1102 26-27 -4.387263543363247 1102 28-29 0.09549728406647517 1102 30-31 -0.3894118680915888 1102 32-33 -5.715816860527944 1102 34-35 -0.44480845675100156 1102 36-37 -0.14615762918466402 1102 38-39 0.23331295882663738 1102 40-41 0.28353485189880345 1102 42-43 0.4778121669377171 1102 44-45 -5.584135861333568 1102 46-47 -0.9291883365912845 1102 48-49 0.3888188371898096 1102 50-51 -5.133177888723338 1102 52-53 -2.8404166446836783 1102 54-55 0.822429528393176 1102 56-57 -2.400621954335559 1102 58-59 0.785652322411412 1102 60-61 0.3207765821185298 1102 62-63 -3.2115227300135203 1102 64-65 -0.519773473512938 1102 66-67 -0.1810079568101024 1102 68-69 -7.208593732732922 1102 70-71 -0.8903844293629568 1102 72-73 -0.25303685563595835 1102 74-75 -5.560852031389505 1102 76 -0.8114632426991797 1103 1 -0.27594634096951154 1103 2 -0.6452320015652084 1103 3 -1.1417517002079016 1103 4 -0.1522255825170049 1103 5 0.06849263628315327 1103 6 -2.1973883758500214 1103 7 -1.0303450540019732 1103 8 0.5508522234301552 1103 9 0.12170753392987166 1103 10-11 0.11797142686701889 1103 12-13 -1.6441648334484285 1103 14-15 -0.731523194715777 1103 16-17 -0.4027895748665564 1103 18-19 -0.8141610584120293 1103 20-21 -0.6142622112504945 1103 22-23 -0.5000422226016212 1103 24-25 -0.27307478148594555 1103 26-27 -1.6942810872228975 1103 28-29 -0.14082882532465035 1103 30-31 -0.255768937235036 1103 32-33 -5.2282007614567405 1103 34-35 -1.3612171254816552 1103 36-37 -1.2591607251598944 1103 38-39 -0.7197314168183553 1103 40-41 0.6209961522084022 1103 42-43 -0.411764716447216 1103 44-45 -3.6739191430673124 1103 46-47 -0.30947729427962045 1103 48-49 -0.4249066530062713 1103 50-51 -0.32874032422385113 1103 52-53 -0.6980017840025639 1103 54-55 0.10571126213930526 1103 56-57 0.5348785616603138 1103 58-59 -0.024461196680434227 1103 60-61 0.6009623406324671 1103 62-63 0.3225329975406588 1103 64-65 0.3357476823178196 1103 66-67 0.7008289884943331 1103 68-69 -2.3943522466648126 1103 70-71 1.005384404486371 1103 72-73 1.12364013094815 1103 74-75 0.1383223752152425 1103 76 1.3350795849581978 1104 1 -2.429868883174077 1104 2 0.039309540970378976 1104 3 1.694395270993688 1104 4 0.40719182893615624 1104 5 1.0943119014271474 1104 6 2.3739456195157516 1104 7 2.2003732511949785 1104 8 2.167535440907809 1104 9 0.24881776233106123 1104 10-11 1.2298549091576554 1104 12-13 0.9275001781371373 1104 14-15 0.9660934885017021 1104 16-17 1.1130992036835998 1104 18-19 1.8678978624750968 1104 20-21 2.005565660315213 1104 22-23 0.747061385475174 1104 24-25 1.8363260791562226 1104 26-27 1.7699691610392634 1104 28-29 1.271782892649533 1104 30-31 0.22453559768717213 1104 32-33 0.5313159548623574 1104 34-35 2.1166047877920953 1104 36-37 1.253583399302201 1104 38-39 0.3822215788784291 1104 40-41 1.9925618589280312 1104 42-43 1.8098506427053778 1104 44-45 -1.8050015661060428 1104 46-47 2.7520917226022057 1104 48-49 1.7669172463648017 1104 50-51 2.865897213015465 1104 52-53 1.531392456315217 1104 54-55 1.48687643923299 1104 56-57 1.680029174040321 1104 58-59 0.42290722437219586 1104 60-61 1.1287677030952246 1104 62-63 2.8132645433864027 1104 64-65 1.7271743622251314 1104 66-67 1.9590216399342602 1104 68-69 -0.5236140804946707 1104 70-71 1.7074698066710816 1104 72-73 3.1147026930136903 1104 74-75 1.1960847055694295 1104 76 0.1844672049515772 1105 1 -0.08296388482915873 1105 2 0.5126627352768978 1105 3 0.5073711068096429 1105 4 0.4091779262549231 1105 5 2.5246329871603486 1105 6 1.504366010114893 1105 7 2.6714093319629377 1105 8 2.287694328693309 1105 9 2.402409288315834 1105 10-11 1.889901251428423 1105 12-13 -1.3634630790624627 1105 14-15 1.4526873316000142 1105 16-17 2.2024735830281834 1105 18-19 1.6156635029914739 1105 20-21 0.7453869115565226 1105 22-23 1.228027952836971 1105 24-25 1.112890130794753 1105 26-27 1.0688768075139379 1105 28-29 0.911802753622716 1105 30-31 0.29686264171233034 1105 32-33 1.1928518701222046 1105 34-35 1.682642523641153 1105 36-37 0.5478568186997563 1105 38-39 0.6662334954623432 1105 40-41 0.5157329943136624 1105 42-43 1.2285861607457633 1105 44-45 2.4488878744765437 1105 46-47 1.2870139337905506 1105 48-49 0.49614597857268095 1105 50-51 0.6449438863024639 1105 52-53 -0.3909842401429131 1105 54-55 0.13202705161299022 1105 56-57 -2.0915763793283304E-4 1105 58-59 -0.9016541791365782 1105 60-61 0.5220149722114122 1105 62-63 0.9628838747336417 1105 64-65 1.0111862788090527 1105 66-67 -0.36057452027759496 1105 68-69 0.8424898585983485 1105 70-71 1.1194194922056688 1105 72-73 0.9394296046323589 1105 74-75 -0.06343201074967197 1105 76 -1.5245695378488193 1106 1 0.03436067657434805 1106 2 0.49292589317163404 1106 3 -1.116532401962285 1106 4 0.16466038239527592 1106 5 0.10138737312525947 1106 6 0.7532695188868246 1106 7 0.8501374021383761 1106 8 0.10896625851787434 1106 9 0.39583034094741265 1106 10-11 -0.3332347134838578 1106 12-13 0.3229404297094618 1106 14-15 -0.8395275806806879 1106 16-17 -0.6549825573226933 1106 18-19 -0.7368584268330807 1106 20-21 -1.3998981761627753 1106 22-23 0.4654183037141699 1106 24-25 0.3502804816719518 1106 26-27 0.9044031233034175 1106 28-29 -1.6704340884825442 1106 30-31 -0.4531373582876661 1106 32-33 0.2186194139818589 1106 34-35 1.0680591903078245 1106 36-37 0.7907296257172973 1106 38-39 1.2106413901991857 1106 40-41 0.04150053817331312 1106 42-43 0.08933177478085241 1106 44-45 0.7087562955291808 1106 46-47 0.7288998987028315 1106 48-49 1.2105100136604001 1106 50-51 0.08189564068842614 1106 52-53 0.7937745317869087 1106 54-55 -1.0697273343519207 1106 56-57 0.965799614291889 1106 58-59 -0.6812594422944684 1106 60-61 0.8158746213342134 1106 62-63 0.8932566817511827 1106 64-65 0.7639275068792237 1106 66-67 1.592276356915388 1106 68-69 1.568367051580804 1106 70-71 -0.06808050779432762 1106 72-73 -0.9350221497536033 1106 74-75 -2.8561951686444047 1106 76 -1.1901397132874152 1107 1 -0.3285779199168779 1107 2 -1.1189162120915235 1107 3 -0.8435060861728125 1107 4 -1.4855589158503406 1107 5 -0.8087003461729871 1107 6 -0.8289673232184427 1107 7 -1.5917485627739048 1107 8 0.32278204799155574 1107 9 -1.5274152730876729 1107 10-11 -1.1715022573435085 1107 12-13 -0.6792525527466751 1107 14-15 -0.4508214403298112 1107 16-17 0.044578846186077214 1107 18-19 -0.4632838654295739 1107 20-21 -0.6932095796715458 1107 22-23 -0.8860071348823233 1107 24-25 0.17429363956668453 1107 26-27 -0.7118249468720208 1107 28-29 -1.8425832112895648 1107 30-31 -0.7908566565332791 1107 32-33 0.026185203455540318 1107 34-35 -0.8787609851307785 1107 36-37 -0.6538975672651617 1107 38-39 -1.4039419431341464 1107 40-41 -0.5281266548091423 1107 42-43 -0.2100103304823051 1107 44-45 -0.8844454588567849 1107 46-47 -0.5901790486655862 1107 48-49 -1.556485600374689 1107 50-51 0.4782772196357925 1107 52-53 0.2756824265237512 1107 54-55 0.2460621393322917 1107 56-57 0.4208434739410123 1107 58-59 0.3965914348985109 1107 60-61 -0.5744762558587659 1107 62-63 -0.3616775287751324 1107 64-65 0.4410108402125523 1107 66-67 -0.421978029049523 1107 68-69 -0.22768557999814476 1107 70-71 -0.7226857709522214 1107 72-73 -1.148289693613254 1107 74-75 -1.0897478002233534 1107 76 -2.1386046255681173 1108 1 -1.0137453840317114 1108 2 0.24822892347466663 1108 3 -1.375244523174409 1108 4 -0.6099608297259387 1108 5 -0.7190671723292859 1108 6 -0.06756381444650827 1108 7 1.1080919475929214 1108 8 0.7479814100330238 1108 9 -1.380684810567736 1108 10-11 -0.5372119862111298 1108 12-13 0.39491012667916436 1108 14-15 0.21823757083446438 1108 16-17 -1.2133158906560304 1108 18-19 -0.24462357834823578 1108 20-21 -0.1383451458597449 1108 22-23 -1.3218923023464377 1108 24-25 -1.2976361849947189 1108 26-27 -2.0024150585147638 1108 28-29 -1.5102068157552715 1108 30-31 -0.024349479499786497 1108 32-33 0.3695663836788228 1108 34-35 -1.0809938399952088 1108 36-37 -0.3030203742827027 1108 38-39 0.7839368447446446 1108 40-41 0.1318414472642253 1108 42-43 0.4631954111444898 1108 44-45 0.09019568946857248 1108 46-47 0.6110968683998017 1108 48-49 -0.518846046945665 1108 50-51 0.22867594371873068 1108 52-53 -1.1692936500312712 1108 54-55 -1.2788182434428315 1108 56-57 0.3002693112615873 1108 58-59 -2.258910957445984 1108 60-61 0.3143594698190668 1108 62-63 0.09988546962997447 1108 64-65 -0.7802012809995666 1108 66-67 -2.0111327339937013 1108 68-69 0.03882159703534782 1108 70-71 1.1709346437208232 1108 72-73 -0.7715751800566366 1108 74-75 0.007062407113167524 1108 76 -0.1283972890449938 1109 1 0.5486150625392625 1109 2 -1.1364600717406468 1109 3 0.10386233487981755 1109 4 -0.8364361088327961 1109 5 -0.5279985917870214 1109 6 0.20612039607980392 1109 7 -1.1005204925984664 1109 8 -1.3263407590259852 1109 9 0.525216305859697 1109 10-11 0.14428721634070385 1109 12-13 -0.6704806229221134 1109 14-15 -0.07362845787367078 1109 16-17 -0.12208782048059064 1109 18-19 -0.8755645671839609 1109 20-21 0.8331062098021391 1109 22-23 0.34206304055627257 1109 24-25 0.26201293781229396 1109 26-27 -0.0451582802053494 1109 28-29 -0.3338112814649996 1109 30-31 -0.6241899898666148 1109 32-33 1.4735536245081775 1109 34-35 0.17387059381659142 1109 36-37 -0.06617826901954516 1109 38-39 0.8065843726553226 1109 40-41 -0.0807582337565087 1109 42-43 -0.40299278662265436 1109 44-45 1.4313440148274204 1109 46-47 -1.4059685223497986 1109 48-49 -0.25823998633960343 1109 50-51 0.1274000266533406 1109 52-53 1.2493666370500662 1109 54-55 0.6846586305603601 1109 56-57 -0.0440688067607411 1109 58-59 -0.015689266855872575 1109 60-61 0.2676290072991314 1109 62-63 -0.6160634936874132 1109 64-65 0.10767750687922373 1109 66-67 0.3060921463890729 1109 68-69 0.4038933673702765 1109 70-71 0.4176651062407579 1109 72-73 0.14995592042183148 1109 74-75 1.4541118488994513 1109 76 1.2824480060108279 1110 1 -0.8137453840317121 1110 2 -0.7517710765253334 1110 3 -0.37524452317440904 1110 4 0.7173118975467894 1110 5 0.4627510094888976 1110 6 -0.17665472353741762 1110 7 1.2717283112292854 1110 8 1.8570723191239331 1110 9 -1.1079575378404662 1110 10-11 -1.7008483498474938 1110 12-13 -1.0050898733208413 1110 14-15 -0.8090351564382665 1110 16-17 -1.4860431633833038 1110 18-19 0.20992187619722102 1110 20-21 -0.29289060040519743 1110 22-23 0.014471334017198245 1110 24-25 -0.0976361849947196 1110 26-27 -0.7478696039693098 1110 28-29 -0.1556613612098161 1110 30-31 -0.23344038859069727 1110 32-33 -0.2758881617757183 1110 34-35 0.009915250913881124 1110 36-37 -0.42120219246451995 1110 38-39 -0.04333588252809051 1110 40-41 -0.8499767345539588 1110 42-43 0.1813772293263085 1110 44-45 0.19928659855947828 1110 46-47 -0.8161758588729242 1110 48-49 0.21751758941797306 1110 50-51 0.5650395800823631 1110 52-53 0.46706998633236196 1110 54-55 -1.0424546070791934 1110 56-57 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-0.47579204533244734 2116 62-63 2.7578400150845184 2116 64-65 3.0139275068792166 2116 66-67 1.717276356915388 2116 68-69 1.7350337182474682 2116 70-71 -1.4326638411276633 2116 72-73 3.0441445169130574 2116 74-75 0.9771381646889274 2116 76 0.8723602867125848 2117 1 -1.4664726567589845 2117 2 -1.2954074401616964 2117 3 -1.4806990686289545 2117 4 0.03549371572860949 2117 5 -2.5536126268747417 2117 6 -2.607563814446511 2117 7 -1.117362597861625 2117 8 -2.4302004081487922 2117 9 -1.2225029923859196 2117 10-11 -2.0644847134838606 2117 12-13 -1.2687262369571997 2117 14-15 -1.4999442473473543 2117 16-17 -1.1633158906560261 2117 18-19 -2.2264417601664164 2117 20-21 -2.3219815094961085 2117 22-23 -0.7891650296191663 2117 24-25 -0.447636184994721 2117 26-27 0.5794031233034147 2117 28-29 -0.4229340884825419 2117 30-31 -0.7298040249543334 2117 32-33 0.16320274731518936 2117 34-35 -1.5473574763588474 2117 36-37 -1.1730203742827001 2117 38-39 -0.5406086098008132 2117 40-41 -2.7772494618266883 2117 42-43 -2.5631682252191474 2117 44-45 0.5929229621958463 2117 46-47 -1.8152667679638341 2117 48-49 -1.1515733196729343 2117 50-51 -0.8176876926449062 2117 52-53 0.7625245317869087 2117 54-55 -1.9797273343519208 2117 56-57 -2.9533670523747766 2117 58-59 -1.1370927756278029 2117 60-61 -0.8874587119991126 2117 62-63 -1.6946599849154822 2117 64-65 -0.8256558264541134 2117 66-67 -1.8902236430846138 2117 68-69 -0.7266329484191978 2117 70-71 -0.817247174460995 2117 72-73 -0.39793881642027173 2117 74-75 -1.1120285019777398 2117 76 -0.41930637995408304 2118 1 0.6311744020645449 2118 2 1.382631775524576 2118 3 1.396947990194576 2118 4 1.170787833375666 2118 5 0.7557991378311435 2118 6 2.673612656141721 2118 7 3.103813872726608 2118 8 -3.4192200159919324 2118 9 -1.2879931884643518 2118 10-11 0.3708094041632002 2118 12-13 1.6097051355918168 2118 14-15 0.37064398794676023 2118 16-17 0.5435468544420097 2118 18-19 0.21473471042181913 2118 20-21 -2.398059940868656 2118 22-23 1.537109480184757 2118 24-25 0.9023638150052804 2118 26-27 1.3970501821269465 2118 28-29 0.9288306173998038 2118 30-31 -0.7913726524053146 2118 32-33 1.6665360806485268 2118 34-35 0.46695624913134637 2118 36-37 1.412665900227104 2118 38-39 0.978410998042321 2118 40-41 -0.33411220692472554 2118 42-43 1.6738905983102654 2118 44-45 1.2786092367056519 2118 46-47 0.23747833007538333 2118 48-49 -0.9445144961435226 2118 50-51 1.6021162289237196 2118 52-53 -1.2717891937032846 2118 54-55 2.0675275676088667 2118 56-57 1.815064320174244 2118 58-59 1.4621229106467055 2118 60-61 -0.7086351825873507 2118 62-63 1.896320407241383 2118 64-65 1.333167702957656 2118 66-67 -2.2594393293591217 2118 68-69 1.6835631300121712 2118 70-71 0.39392929612724004 2118 72-73 0.3450023600503158 2118 74-75 1.7724813019438272 2118 76 0.0316740122027781 2119 1 0.3260273432410159 2119 2 0.36792589317163404 2119 3 -0.28319906862895294 2119 4 0.37299371572860807 2119 5 0.6847207064585916 2119 6 1.5241028522201532 2119 7 -3.024862597861624 2119 8 1.4839662585178708 2119 9 0.35416367428074835 2119 10-11 -0.5207347134838578 2119 12-13 -1.0520595702905382 2119 14-15 -1.3499442473473557 2119 16-17 0.8033507760106353 2119 18-19 -0.3931084268330842 2119 20-21 -3.3790648428294396 2119 22-23 -0.16999836295249793 2119 24-25 -0.6601361849947196 2119 26-27 0.25856978997008184 2119 28-29 -0.7329340884825442 2119 30-31 -0.1718873582876661 2119 32-33 -0.8542972526848089 2119 34-35 -0.5881908096921791 2119 36-37 0.030312959050633026 2119 38-39 -1.4768586098008143 2119 40-41 -0.6251661284933547 2119 42-43 -0.5773348918858119 2119 44-45 -3.4266603711374906 2119 46-47 -1.333600101297165 2119 48-49 -0.6853233196729356 2119 50-51 0.748562307355094 2119 52-53 -3.7270588015464234 2119 54-55 -2.465560667685253 2119 56-57 -0.7008670523747753 2119 58-59 -0.045842775627804144 2119 60-61 -0.5799587119991152 2119 62-63 -0.7838266515821459 2119 64-65 -0.256905826454112 2119 66-67 -0.25147364308461206 2119 68-69 -0.4316329484191961 2119 70-71 -2.3076638411276598 2119 72-73 -3.2058554830869355 2119 74-75 -2.116611835311076 2119 76 -2.065139713287415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 7.0 13 87.0 14 193.0 15 371.0 16 417.0 17 522.0 18 523.0 19 500.0 20 430.0 21 341.0 22 282.0 23 249.0 24 264.0 25 223.0 26 260.0 27 334.0 28 389.0 29 420.0 30 512.0 31 637.0 32 801.0 33 1055.0 34 1411.0 35 2135.0 36 3438.0 37 3538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.145833333333332 29.260416666666668 10.005208333333334 29.588541666666668 2 15.166244376648224 21.55747453332644 34.22100418842753 29.05527690159781 3 18.139510833031697 25.80278194322354 28.21759139562542 27.840115828119345 4 11.381146905217436 20.394022441698123 39.67630177361808 28.54852887946636 5 15.0524846165779 36.22214178602823 31.80102383784063 16.924349759553234 6 24.36527224778944 36.68752262267956 16.47965251564197 22.467552613889033 7 22.302083871968563 30.27043797507627 23.51207404726201 23.91540410569316 8 19.654584001241016 33.92626299188169 18.692796938828273 27.726356068049018 9 20.96799214023476 17.17255287243394 21.050726511194995 40.8087284761363 10-11 22.19608045917576 25.588189668545425 26.679249185583537 25.536480686695278 12-13 21.06623920575004 23.26645638347381 26.51895134184808 29.148353068928074 14-15 19.18920316458969 25.93981074512643 23.811986141992865 31.058999948291017 16-17 21.787579502559595 27.519520140648428 24.628988055225193 26.063912301566784 18-19 20.148404777909924 25.80278194322354 25.89068721236879 28.15812606649775 20-21 19.765758312218832 27.204095351362533 25.050416257303894 27.979730079114738 22-23 22.674388541289623 26.555147629143182 24.605719013392626 26.16474481617457 24-25 21.102435493045142 28.61316510677905 23.95160039298826 26.332799007187546 26-27 20.647396452763843 28.92858989606495 25.1926159573918 25.23139769377941 28-29 21.640208904286677 26.291431821707427 27.537618284295984 24.530740989709912 30-31 21.110191840322663 27.684988882568902 26.640467449195924 24.56435182791251 32-33 19.359842804695177 29.205232948963232 26.20869745074719 25.226226795594396 34-35 20.293189927090335 28.0004136718548 26.128548528879463 25.577847872175397 36-37 21.00677387662237 27.674647086198874 25.77434200320596 25.5442370339728 38-39 20.874915972904493 26.069083199751798 28.160711515590258 24.89528931175345 40-41 19.574435079373288 26.348311701742595 28.02368271368737 26.053570505196756 42-43 19.69853663581364 25.453746315735042 28.538187083096332 26.309529965354983 44-45 18.359274005894825 25.08144164641398 29.212989296240753 27.346295051450436 46-47 18.483375562335176 26.221624696209734 28.84844097419722 26.446558767257873 48-49 18.72899322612338 27.860799420859404 27.563472775221054 25.846734577796166 50-51 18.11624179119913 27.284244273230264 27.410931278763123 27.188582656807487 52-53 17.650860954547806 27.56864367340607 27.754796008066602 27.025699363979523 54-55 17.030353172346036 27.876312115414446 27.398004033300584 27.695330678938934 56-57 17.966285743833705 27.372149542375514 27.077408345829674 27.584156367961114 58-59 18.02833652205388 26.78008170019132 26.888670562076634 28.30291121567816 60-61 17.681886343657894 26.167330265267076 26.51895134184808 29.631832049226954 62-63 18.664356998810693 25.769171105020945 26.689590981953565 28.876880914214798 64-65 19.25901029008739 26.237137390764776 25.197786855576815 29.306065463571024 66-67 19.41672268473034 27.511763793370907 24.168778116758883 28.90273540513987 68-69 19.57184963028078 28.04953720461244 24.104141889446197 28.274471275660584 70-71 21.30927142044573 26.97916128031439 24.104141889446197 27.607425409793677 72-73 21.148973576710276 27.025699363979523 23.88696416567558 27.938362893634622 74-75 21.027457469362428 28.077977144630022 23.881793267490565 27.01277211851699 76 20.905941362014584 26.90935415481669 24.530740989709912 27.653963493458818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.5 15 1.5 16 2.5 17 4.0 18 5.0 19 5.5 20 7.0 21 9.0 22 15.0 23 24.5 24 32.5 25 37.0 26 53.5 27 71.5 28 90.5 29 108.0 30 127.0 31 161.0 32 219.0 33 262.0 34 301.0 35 371.0 36 463.0 37 524.0 38 593.0 39 752.0 40 919.5 41 1011.5 42 1026.0 43 1072.0 44 1220.5 45 1354.5 46 1386.0 47 1356.0 48 1284.0 49 1226.0 50 1210.0 51 1119.5 52 943.0 53 830.0 54 803.0 55 769.0 56 653.5 57 524.5 58 477.0 59 434.0 60 345.5 61 265.0 62 230.0 63 186.5 64 146.5 65 125.5 66 90.0 67 79.0 68 55.5 69 29.0 70 21.0 71 16.0 72 13.5 73 9.5 74 6.0 75 4.0 76 3.5 77 3.5 78 5.5 79 7.0 80 4.5 81 1.0 82 1.0 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7187548477170485 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 19339.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.81658824137752 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2087771623349 58.40529499974145 2 10.545377077119728 15.357567609493769 3 4.956682289447522 10.82786079942086 4 2.2653032239738673 6.598066084078805 5 1.0012782275244994 3.6454832204353895 6 0.5325948018747336 2.3269041832566315 7 0.2343417128248828 1.1944774807384044 8 0.14912654452492544 0.8687108950824758 9 0.04260758414997869 0.2792285019907958 >10 0.06391137622496805 0.4964062257614148 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.025854490925073685 0.0 2 0.0 0.0 0.0 0.03619628729510316 0.0 3 0.0 0.0 0.0 0.04653808366513263 0.0 4 0.0 0.0 0.0 0.08790526914525053 0.0 5 0.0 0.0 0.0 0.09824706551528001 0.0 6 0.0 0.0 0.0 0.09824706551528001 0.0 7 0.0 0.0 0.0 0.10858886188530947 0.0 8 0.0 0.0 0.0 0.13444335281038317 0.0 9 0.0 0.0 0.0 0.17063964010548632 0.0 10 0.0 0.0 0.0 0.19649413103056002 0.0 11 0.0 0.0 0.0 0.24303221469569264 0.0 12 0.0 0.0 0.0 0.2792285019907958 0.0 13 0.0 0.0 0.0 0.28439940017581056 0.0 14 0.0 0.0 0.0 0.29991209473085473 0.0 15 0.0 0.0 0.0 0.3205956874709137 0.0 16 0.0 0.0 0.0 0.3257665856559284 0.0 17 0.0 0.0 0.0 0.3464501783959874 0.0 18 0.0 0.0 0.0 0.3516210765810021 0.0 19 0.0 0.0 0.0 0.3774755675060758 0.0 20 0.0 0.0 0.0 0.4188427529861937 0.0 21 0.0 0.0 0.0 0.4446972439112674 0.0 22 0.0 0.0 0.0 0.49640622576141474 0.0 23 0.0 0.0 0.0 0.49640622576141474 0.0 24 0.0 0.0 0.0 0.5584570039815916 0.0 25 0.0 0.0 0.0 0.5739696985366358 0.0 26 0.0 0.0 0.0 0.5791405967216505 0.0 27 0.0 0.0 0.0 0.5946532912766948 0.0 28 0.0 0.0 0.0 0.6360204767568126 0.0 29 0.0 0.0 0.0 0.6567040694968717 0.0 30 0.0 0.0 0.0 0.6980712549769895 0.0 31 0.0 0.0 0.0 0.7446093386421221 0.0 32 0.0 0.0 0.0 0.7859765241222401 0.0 33 0.0 0.0 0.0 0.8790526914525053 0.0 34 0.0 0.0 0.0 0.9669579605977559 0.0 35 0.0 0.0 0.0 1.1686229898133307 0.0 36 0.0 0.0 0.0 1.3806298153989347 0.0 37 0.0 0.0 0.0 1.7787889756450697 0.0 38 0.0 0.0 0.0 2.2389989141113813 0.0 39 0.0 0.0 0.0 2.8801902890532087 0.0 40 0.0 0.0 0.0 3.8006101659858316 0.0 41 0.0 0.0 0.0 4.540048606442939 0.0 42 0.0 0.0 0.0 4.989916748539221 0.0 43 0.0 0.0 0.0 5.693158901701225 0.0 44 0.0 0.0 0.0 6.308495785717979 0.0 45 0.0 0.0 0.0 7.016908837064999 0.0 46 0.0 0.0 0.0 7.926986917627592 0.0 47 0.0 0.0 0.0 8.563007394384405 0.0 48 0.0 0.0 0.0 9.240395056621335 0.0 49 0.0 0.0 0.0 9.767826671492838 0.0 50 0.0 0.0 0.0 10.450385231914783 0.0 51 0.0 0.0 0.0 10.988158643156316 0.0 52 0.0 0.0 0.0 11.598324628988054 0.0 53 0.0 0.0 0.0 12.19297792026475 0.0 54 0.0 0.0 0.0 12.66352965510109 0.0 55 0.0 0.0 0.0 13.149594084492476 0.0 56 0.0 0.0 0.0 13.625316717513833 0.0 57 0.0 0.0 0.0 14.199286416050468 0.0 58 0.0 0.0 0.0 14.814623300067222 0.0 59 0.0 0.0 0.0 15.290345933088577 0.0 60 0.0 0.0 0.0 15.84880293707017 0.0 61 0.0 0.0 0.0 16.215936708206215 0.0 62 0.0 0.0 0.0 16.608924970267335 0.0 63 0.0 0.0 0.0 17.06396401054863 0.0 64 0.0 0.0 0.0 17.451781374424737 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCAGGG 20 0.006851445 52.496113 32 CACTATC 20 0.006851445 52.496113 32 TTGCTGT 35 0.0011947176 40.205357 1 >>END_MODULE