FastQCFastQC Report
Wed 25 May 2016
SRR1780712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19339
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT11646.018925487357154No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT5252.714721547132737TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1380.7135839495320337No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1150.5946532912766948No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT580.2999120947308548No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC480.2482031128807074No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCGT400.20683592740058948No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT390.20166502921557475No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT360.18615233466053052No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC220.1137597600703242No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC200.10341796370029474No Hit
ACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCT200.10341796370029474RNA PCR Primer, Index 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA150.00219181770.000012
TCTACTG207.73309E-569.999992
GTCTACT207.73309E-569.999991
GTGTATA200.006849439352.4999961
TATAAGA200.006849439352.4999964
CGTCTTC2850.038.07017548
CTGCTTG2800.037.554
TCTTCTG2800.037.550
CTTCTGC2800.037.551
CCGTCTT2900.037.4137947
GCCGTCT2900.037.4137946
TATGCCG3000.037.33333243
GTCTTCT2850.036.84210249
ATGCCGT2950.036.77966344
CGTATGC2950.036.77966341
TCGTATG2950.036.77966340
CGTATCT2500.036.39999834
TGAAAAA2600.036.34615359
TTCTGCT2900.036.20689852
GCACGTA2900.036.20689831
Produced by FastQC (version 0.11.5)