##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780712_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19339 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.97316303841977 34.0 34.0 34.0 32.0 34.0 2 33.3153213713222 34.0 34.0 34.0 33.0 34.0 3 33.50018098143648 34.0 34.0 34.0 34.0 34.0 4 33.50519675267594 34.0 34.0 34.0 34.0 34.0 5 33.490614819794196 34.0 34.0 34.0 34.0 34.0 6 37.258079528414086 38.0 37.0 38.0 36.0 38.0 7 36.83132530120482 38.0 37.0 38.0 35.0 38.0 8 36.92073013082372 38.0 37.0 38.0 35.0 38.0 9 36.67568126583588 38.0 37.0 38.0 34.0 38.0 10-11 36.37701018666942 38.0 37.0 38.0 33.0 38.0 12-13 36.23649102849165 38.0 37.0 38.0 34.0 38.0 14-15 36.47704121205854 38.0 37.0 38.0 34.0 38.0 16-17 36.57883034283055 38.0 37.0 38.0 34.0 38.0 18-19 36.54480583277315 38.0 37.0 38.0 34.0 38.0 20-21 36.273023424168784 38.0 37.0 38.0 33.5 38.0 22-23 36.373700811831014 38.0 37.0 38.0 33.5 38.0 24-25 35.62919489115259 38.0 36.0 38.0 31.5 38.0 26-27 35.81268421324784 38.0 36.0 38.0 31.5 38.0 28-29 35.698097109467916 38.0 36.5 38.0 31.0 38.0 30-31 35.29326749056311 38.0 36.0 38.0 29.0 38.0 32-33 35.863048761569885 38.0 36.5 38.0 31.5 38.0 34-35 35.83153213713222 38.0 36.5 38.0 31.5 38.0 36-37 35.938518020580176 38.0 37.0 38.0 33.0 38.0 38-39 35.065515280004135 38.0 35.5 38.0 29.0 38.0 40-41 35.013702880190294 37.0 35.5 38.0 29.0 38.0 42-43 35.621076581002114 38.0 36.5 38.0 32.5 38.0 44-45 35.85381870830963 38.0 36.5 38.0 32.5 38.0 46-47 36.12536842649568 38.0 37.0 38.0 34.0 38.0 48-49 35.89940017581054 38.0 36.5 38.0 32.5 38.0 50-51 36.006230932312945 38.0 37.0 38.0 32.5 38.0 52-53 35.854956305910335 38.0 37.0 38.0 32.0 38.0 54-55 36.070634469207306 38.0 37.0 38.0 34.0 38.0 56-57 36.167666373649105 38.0 37.0 38.0 34.0 38.0 58-59 36.10088422358964 38.0 37.0 38.0 34.0 38.0 60-61 35.832437044314595 38.0 36.5 38.0 32.5 38.0 62-63 36.155980143750966 38.0 37.0 38.0 33.5 38.0 64-65 36.17924918558353 38.0 37.0 38.0 33.5 38.0 66-67 35.385180205801746 38.0 36.0 38.0 31.0 38.0 68-69 34.33988313770102 37.5 34.5 38.0 24.0 38.0 70-71 33.84133098919283 37.0 34.0 38.0 22.5 38.0 72-73 32.975050416257304 37.0 33.0 38.0 16.0 38.0 74-75 33.31516624437664 37.5 34.0 38.0 19.0 38.0 76 32.777030870262166 37.0 32.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6102401445088859 1101 2 0.40863362340923004 1101 3 0.43892309935817764 1101 4 0.26531657996235225 1101 5 0.12907649778246366 1101 6 -0.5580047010124289 1101 7 0.05519939106634553 1101 8 0.24215707973004186 1101 9 0.5137275117680105 1101 10-11 -0.04951117414384498 1101 12-13 0.26809916722031346 1101 14-15 1.1488154442786254 1101 16-17 0.6631311062168166 1101 18-19 0.17388131639569337 1101 20-21 0.11659696014996968 1101 22-23 0.6910870483894058 1101 24-25 -0.01503969092814117 1101 26-27 0.802906315449448 1101 28-29 0.9826785199481165 1101 30-31 1.729452096725261 1101 32-33 1.0860162319050772 1101 34-35 1.2019876522850481 1101 36-37 1.0016453243587904 1101 38-39 1.7425864943751748 1101 40-41 2.3301517844045136 1101 42-43 1.2456455166640978 1101 44-45 0.6735169593810539 1101 46-47 1.438640789780031 1101 48-49 -0.13459113775073206 1101 50-51 -0.9236852128431678 1101 52-53 -0.6815839832286272 1101 54-55 -0.8932609930780586 1101 56-57 -0.437312558688987 1101 58-59 -0.626627366374592 1101 60-61 -0.9726405998745307 1101 62-63 -0.8978158918968262 1101 64-65 -0.9550537320980297 1101 66-67 -1.1900986461322276 1101 68-69 0.1531315538510185 1101 70-71 -0.6997722384569798 1101 72-73 -0.16772668717275963 1101 74-75 1.04850333300422 1101 76 0.2872135234667539 1102 1 -1.7757892672558206 1102 2 0.03240813321315272 1102 3 0.4229917268091583 1102 4 0.47364991329568795 1102 5 0.4452529683706956 1102 6 0.49714235781110006 1102 7 0.09931703812517156 1102 8 0.24828453071043555 1102 9 -0.1995077823496345 1102 10-11 0.45906725722869623 1102 12-13 0.3342756378085454 1102 14-15 -0.37324337925078055 1102 16-17 0.40026345915799055 1102 18-19 -0.7654569188984297 1102 20-21 0.42970970524800833 1102 22-23 0.3577537150560701 1102 24-25 -4.363078906614415 1102 26-27 -0.9268730963152478 1102 28-29 0.10522753955596187 1102 30-31 1.2061677829997706 1102 32-33 -0.24731710142825136 1102 34-35 0.008360201304654424 1102 36-37 -2.6699233030921903 1102 38-39 -0.3413595840561996 1102 40-41 0.7431419804829389 1102 42-43 0.8730964970562596 1102 44-45 0.9535414691849837 1102 46-47 0.6377829466427798 1102 48-49 -3.7516254514762295 1102 50-51 -1.0070185461765035 1102 52-53 -0.22263790479725998 1102 54-55 1.043013516725864 1102 56-57 0.7648933236639479 1102 58-59 0.029622633625407957 1102 60-61 -4.242861188109821 1102 62-63 -2.672325695818394 1102 64-65 -0.5034605948431263 1102 66-67 1.1113719421030765 1102 68-69 -2.7966233481097618 1102 70-71 -3.1862918463001115 1102 72-73 -4.925692373447273 1102 74-75 -1.5446339218977378 1102 76 -0.5706296137881495 1103 1 0.9073892673159065 1103 2 0.2759581848127439 1103 3 0.3610722221651983 1103 4 0.21049201855884547 1103 5 0.48653263813334036 1103 6 0.4145830182858177 1103 7 0.7701116717680989 1103 8 0.4866746235896926 1103 9 0.1782011959785379 1103 10-11 0.25421689603159336 1103 12-13 -0.5542692538323237 1103 14-15 -0.34899157326522356 1103 16-17 0.07157408867295345 1103 18-19 0.45074535148340544 1103 20-21 0.24653117067629182 1103 22-23 0.6910870483894129 1103 24-25 -2.870302848822874 1103 26-27 -0.5331682459540588 1103 28-29 -0.4866197256659177 1103 30-31 0.8950222721638568 1103 32-33 1.0947881617296389 1103 34-35 0.36646133649557555 1103 36-37 -1.2922143247640179 1103 38-39 -0.7020406986072771 1103 40-41 -0.22796225068320553 1103 42-43 -0.2280386938622172 1103 44-45 0.9076178365740404 1103 46-47 0.7538820178502093 1103 48-49 -1.2118937693296843 1103 50-51 -1.0596501251238735 1103 52-53 -0.2917813516496821 1103 54-55 -0.6608048527271819 1103 56-57 -0.6050757165837268 1103 58-59 -0.3124826295324894 1103 60-61 -6.040074810400842 1103 62-63 -2.125886067335422 1103 64-65 0.011503285445833455 1103 66-67 -1.380888119816433 1103 68-69 -0.571649147903365 1103 70-71 0.7048330247009247 1103 72-73 -5.825073178400835 1103 74-75 -1.4471107020834921 1103 76 -4.190856651971842 1104 1 0.4062307105466232 1104 2 0.6039952586293609 1104 3 0.5014230993581776 1104 4 0.17176312084285428 1104 5 0.12671800721642512 1104 6 0.3920739153397719 1104 7 1.0064572527015656 1104 8 0.6922356960822427 1104 9 1.18118034195669 1104 10-11 -0.3942438785463622 1104 12-13 0.20756457602534084 1104 14-15 0.46878399773774504 1104 16-17 0.7258276470973115 1104 18-19 1.2271438950120412 1104 20-21 0.05901048216254878 1104 22-23 0.6281939666284018 1104 24-25 0.4708093656756347 1104 26-27 0.9784173217387604 1104 28-29 1.3836219161745333 1104 30-31 0.7707256816309282 1104 32-33 1.1017395023453318 1104 34-35 0.19962916171900957 1104 36-37 1.638437777188983 1104 38-39 1.7463207711047417 1104 40-41 1.6119914070460268 1104 42-43 0.7519348248402054 1104 44-45 1.2515436889408065 1104 46-47 0.5787744375787796 1104 48-49 -0.004677615738160057 1104 50-51 0.955245604766894 1104 52-53 0.6393672746330097 1104 54-55 0.5831541012615631 1104 56-57 0.3335207746443416 1104 58-59 -0.7691195047393791 1104 60-61 0.9021628592449744 1104 62-63 0.1084734162792742 1104 64-65 0.8886962679019703 1104 66-67 0.007228397892930616 1104 68-69 1.8060403588824698 1104 70-71 2.642601975379499 1104 72-73 1.777045325405851 1104 74-75 0.41800018835013475 1104 76 -0.7741072312502268 1105 1 0.995108565561516 1105 2 0.39876520235659996 1105 3 -0.3406821637997197 1105 4 0.2806674571553387 1105 5 0.3110940416421144 1105 6 -0.024013472942250758 1105 7 0.6473046542242429 1105 8 -0.5308692360594307 1105 9 0.8097801433469556 1105 10-11 0.7366730363824701 1105 12-13 -0.6507604819024948 1105 14-15 -0.2700442048441758 1105 16-17 -0.18281187623932738 1105 18-19 0.24899096551849453 1105 20-21 -0.18329339072722206 1105 22-23 -0.16856207441760773 1105 24-25 0.3753111862648453 1105 26-27 -0.4542208775330039 1105 28-29 -0.12697060285889705 1105 30-31 1.0178292897077128 1105 32-33 0.6912793897998171 1105 34-35 -1.098450944206185 1105 36-37 0.21655760506054378 1105 38-39 0.0874329856032503 1105 40-41 -0.4034008471744315 1105 42-43 -0.27189834298502547 1105 44-45 0.3813020471003554 1105 46-47 0.9293206143414423 1105 48-49 -0.08908675178582826 1105 50-51 0.3175428573322705 1105 52-53 0.12927127992926302 1105 54-55 0.13744076130790717 1105 56-57 1.0440470904338142 1105 58-59 -0.26862298040968824 1105 60-61 0.144135715914949 1105 62-63 -1.2399211550547236 1105 64-65 0.3360646889546004 1105 66-67 0.27700661702567686 1105 68-69 0.1388771678861076 1105 70-71 0.6434295159289931 1105 72-73 -0.21103809068153367 1105 74-75 -0.4558826319080538 1105 76 0.4056345760983362 1106 1 -2.618926522157782 1106 2 -0.25803304325743426 1106 3 -0.24857690064182236 1106 4 -0.19301675337097635 1106 5 -1.1834235022175363 1106 6 -0.47467136767909324 1106 7 -1.278133942266983 1106 8 0.03382374639671326 1106 9 -1.8196058215653252 1106 10-11 0.4400721591894836 1106 12-13 0.7472658338869778 1106 14-15 -0.20535122238803893 1106 16-17 0.006881106216809485 1106 18-19 0.507214649729022 1106 20-21 -0.7375697065166946 1106 22-23 0.08692038172274152 1106 24-25 -1.4837896909281412 1106 26-27 0.21957298211611942 1106 28-29 0.4097618532814451 1106 30-31 0.6357020967252609 1106 32-33 0.28393289857174864 1106 34-35 0.4415709856183767 1106 36-37 0.553728657692119 1106 38-39 -0.5594968389581609 1106 40-41 -0.565681548928822 1106 42-43 -0.21268781666923076 1106 44-45 0.9131002927143896 1106 46-47 0.17822412311337388 1106 48-49 1.1883255289159322 1106 50-51 -0.548685212843175 1106 52-53 0.46424935010470136 1106 54-55 0.5546556735886128 1106 56-57 0.1460207746443416 1106 58-59 0.35253930029206515 1106 60-61 -0.29555726654118786 1106 62-63 0.4771841081031667 1106 64-65 -2.1738037320980297 1106 66-67 -1.638015312798892 1106 68-69 0.5385482205176828 1106 70-71 -0.36643890512363697 1106 72-73 0.3739399794939047 1106 74-75 -0.49316333366244436 1106 76 -0.5461198098665818 1107 1 0.5214243550352009 1107 2 -0.4082523415030508 1107 3 0.20317748532308855 1107 4 0.17540429926059886 1107 5 0.11811158550176515 1107 6 0.4496707375840643 1107 7 0.594673075276873 1107 8 -0.33788677991908145 1107 9 -0.08495669875830458 1107 10-11 0.7805326855052712 1107 12-13 -0.1507604819024948 1107 14-15 0.3439908828751186 1107 16-17 0.9136793518308437 1107 18-19 0.9770611409570904 1107 20-21 0.1763557320797986 1107 22-23 -0.29136909196146377 1107 24-25 -0.21240811198077125 1107 26-27 -0.2700103512172092 1107 28-29 0.8905732567902263 1107 30-31 0.5704608686550827 1107 32-33 0.6474197406770088 1107 34-35 0.3226016873727673 1107 36-37 1.5674347980430028 1107 38-39 0.3242750908664007 1107 40-41 0.10537108265013018 1107 42-43 0.5614349903483102 1107 44-45 0.793582748854746 1107 46-47 0.8240574564467025 1107 48-49 0.8231939499685552 1107 50-51 1.1859639099638528 1107 52-53 0.7783940869468111 1107 54-55 0.38305479639562634 1107 56-57 0.77211726587241 1107 58-59 0.6085700020464557 1107 60-61 0.5651883474938941 1107 62-63 0.4004297221382629 1107 64-65 0.7746611801826688 1107 66-67 -0.12650215490415206 1107 68-69 1.6213333082369843 1107 70-71 -0.16358802793065763 1107 72-73 -0.05314335383943103 1107 74-75 -1.999742281030862 1107 76 -1.2084005116209653 1108 1 -1.6431689464002055 1108 2 0.49764877492438586 1108 3 -0.2985769006418195 1108 4 0.38274082238659446 1108 5 -0.1686507749448083 1108 6 -0.014444094951819864 1108 7 -0.22510363923668564 1108 8 -1.2051914051184411 1108 9 -0.520363397322896 1108 10-11 0.3054130682803944 1108 12-13 0.9173415914627299 1108 14-15 0.5950275654907529 1108 16-17 -0.7541037422680361 1108 18-19 0.12937374063811546 1108 20-21 0.47890756621058017 1108 22-23 -0.384670527368165 1108 24-25 0.8804148545263999 1108 26-27 -0.5275861087929741 1108 28-29 -1.2309578436882518 1108 30-31 0.1033157330888983 1108 32-33 0.3708647167535659 1108 34-35 0.422442197739592 1108 36-37 1.5789180516315184 1108 38-39 0.9094425549812399 1108 40-41 0.3176517844045108 1108 42-43 -0.1331423621237775 1108 44-45 0.4551457472598486 1108 46-47 0.8422392746285183 1108 48-49 1.2334012864916843 1108 50-51 0.4475269083689497 1108 52-53 -0.2704097408043893 1108 54-55 -0.9159882658053249 1108 56-57 -0.49375195262838645 1108 58-59 -0.44613494213217564 1108 60-61 0.6184578849739566 1108 62-63 1.1961235020425605 1108 64-65 0.5250599042656106 1108 66-67 0.5197498387162653 1108 68-69 1.5103285235479902 1108 70-71 -1.1433328445175803 1108 72-73 -0.8531433538394353 1108 74-75 -1.8893754548745605 1108 76 -0.03551374926051665 1109 1 -1.706645820403395 1109 2 0.2057827462162507 1109 3 0.4137038011125611 1109 4 0.298211316804462 1109 5 0.06548000655439523 1109 6 -0.4275222448720726 1109 7 -0.19480060893365447 1109 8 0.030534272712500865 1109 9 0.2834643538732706 1109 10-11 0.4296554925228193 1109 12-13 0.9808184654659229 1109 14-15 0.7124119355067009 1109 16-17 0.8785916325325971 1109 18-19 0.20513131639569337 1109 20-21 1.0798645040096204 1109 22-23 0.6472273992666047 1109 24-25 0.19110065994905057 1109 26-27 0.4142001750985713 1109 28-29 -0.2673214800518835 1109 30-31 0.3160749037428019 1109 32-33 0.8579460564664885 1109 34-35 -0.6773983126272327 1109 36-37 1.2516453243587904 1109 38-39 1.2628715820944763 1109 40-41 0.8597570475624039 1109 42-43 1.043891130699194 1109 44-45 1.3286704681529855 1109 46-47 0.4293206143414423 1109 48-49 1.2003869324247063 1109 50-51 0.6947358397884074 1109 52-53 -0.2917813516496821 1109 54-55 -0.3274715193938533 1109 56-57 0.4124681430653965 1109 58-59 -0.2949387698833732 1109 60-61 0.48624097907283925 1109 62-63 -0.31886852347577843 1109 64-65 0.9676436363230252 1109 66-67 0.3033224064993618 1109 68-69 -0.7997193233419608 1109 70-71 -0.1723599577552264 1109 72-73 0.4819443654588156 1109 74-75 -0.5348300003291087 1109 76 0.28282755855447306 1110 1 -0.5340780373092926 1110 2 0.134012411288019 1110 3 0.2105140084490884 1110 4 0.29183173147750807 1110 5 0.5040764977824637 1110 6 -0.19626227676999974 1110 7 0.5021690880360481 1110 8 0.2675358676088351 1110 9 0.806909329949832 1110 10-11 -0.3673142044468847 1110 12-13 0.40825068237182194 1110 14-15 0.8132093836725716 1110 16-17 0.23680534864105596 1110 18-19 -0.25244444118007436 1110 20-21 1.006180293483304 1110 22-23 -0.884670527368165 1110 24-25 -0.5104942363826908 1110 26-27 1.3724138912070316 1110 28-29 0.82358761085721 1110 30-31 -0.22395699418382975 1110 32-33 0.47995562584447526 1110 34-35 1.3769876522850453 1110 36-37 1.5152816879951558 1110 38-39 0.06398800952668893 1110 40-41 -2.4732573065045784 1110 42-43 -0.11496054394196165 1110 44-45 0.6187821108962055 1110 46-47 0.5240574564467053 1110 48-49 1.4424921955825951 1110 50-51 -0.7070185461765064 1110 52-53 -0.19768246807711165 1110 54-55 -0.6523519021689737 1110 56-57 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48-49 0.44874219558259654 2116 50-51 0.02423145382349645 2116 52-53 -0.20241731656197004 2116 54-55 -0.3411776597447229 2116 56-57 0.4585207746443416 2116 58-59 -1.5953773663745991 2116 60-61 0.11069273345881214 2116 62-63 1.143850774769838 2116 64-65 -1.777970398764694 2116 66-67 -0.1484319794655562 2116 68-69 0.5593815538510256 2116 70-71 3.0918944282096916 2116 72-73 2.384356646160569 2116 74-75 2.17350333300422 2116 76 2.891380190133418 2117 1 1.0477401445088859 2117 2 0.5994669567425674 2117 3 0.4814230993581745 2117 4 0.2536499132956891 2117 5 0.2840764977824648 2117 6 -0.14717136767909267 2117 7 0.25853272439967867 2117 8 0.7529904130633795 2117 9 -0.21127248823199096 2117 10-11 0.44632215918948503 2117 12-13 -0.35356749944635624 2117 14-15 4.821109452990413E-4 2117 16-17 -0.5622855604498582 2117 18-19 -1.3015353502709814 2117 20-21 0.3761802934833014 2117 22-23 0.5144203817227364 2117 24-25 -1.48503969092814 2117 26-27 -0.9366770178838806 2117 28-29 -0.29732148005188463 2117 30-31 -0.5230479032747368 2117 32-33 -1.927317101428251 2117 34-35 0.45698765228504357 2117 36-37 -1.5583546756412119 2117 38-39 -0.8278301722914918 2117 40-41 0.02765178440451166 2117 42-43 0.17231218333076725 2117 44-45 0.11060029271438765 2117 46-47 0.24405745644670418 2117 48-49 -0.2975078044174069 2117 50-51 -0.24701854617650554 2117 52-53 0.3804993501047065 2117 54-55 -0.0432609930780643 2117 56-57 0.44352077464434103 2117 58-59 -0.6370440330412634 2117 60-61 -0.48972393320786267 2117 62-63 0.46885077476984094 2117 64-65 -0.49130373209802514 2117 66-67 -0.10843197946555705 2117 68-69 -0.36603511281564494 2117 70-71 -1.8106055717903118 2117 72-73 -0.9631433538394276 2117 74-75 -1.6748300003291092 2117 76 -0.9827864765332457 2118 1 -0.8542206398048364 2118 2 0.24809440772295943 2118 3 -1.1652435673084867 2118 4 0.23835579564862996 2118 5 0.4844686546452053 2118 6 0.30106392643855173 2118 7 1.0797091949879132 2118 8 0.7580884522790612 2118 9 -2.3171548411731635 2118 10-11 -0.46250137022228444 2118 12-13 -1.5872929896424353 2118 14-15 0.7051879932982388 2118 16-17 -3.2173835996655384 2118 18-19 -0.608594173800391 2118 20-21 -0.4624471574970883 2118 22-23 0.17147920525215454 2118 24-25 0.48005834828754246 2118 26-27 -1.6425593708250545 2118 28-29 -0.0026155976989414853 2118 30-31 -1.4604988836668937 2118 32-33 -0.3551602386831547 2118 34-35 0.03777196601053845 2118 36-37 -3.444433107013758 2118 38-39 -0.6060654664091345 2118 40-41 -0.2764658626543124 2118 42-43 0.6868219872523369 2118 44-45 -3.7229291190503133 2118 46-47 0.29464569174081845 2118 48-49 0.18955101911200956 2118 50-51 -0.36976364421571617 2118 52-53 -1.693226140091376 2118 54-55 -1.2413002087643363 2118 56-57 0.29430508836983194 2118 58-59 -0.735083248727534 2118 60-61 -0.48795922732551134 2118 62-63 -0.7213453036615292 2118 64-65 0.7416374443725644 2118 66-67 0.22901900092659844 2118 68-69 -0.9044664853646651 2118 70-71 0.8921395262489042 2118 72-73 1.0252880187095883 2118 74-75 0.6906601957493237 2118 76 2.213684111702051 2119 1 0.5477401445088859 2119 2 -0.46636637659076996 2119 3 -0.33191023397515806 2119 4 -0.42218342003764775 2119 5 -0.24592350221753634 2119 6 -0.09967136767909324 2119 7 -1.1114672756003188 2119 8 -0.29950958693662244 2119 9 -1.1529391548986538 2119 10-11 -0.04951117414384498 2119 12-13 0.17434916722031346 2119 14-15 0.07589877761196817 2119 16-17 -0.6077022271165191 2119 18-19 0.017631316395693375 2119 20-21 -0.4563197065166946 2119 22-23 0.28483704838941293 2119 24-25 0.9224603090718588 2119 26-27 0.6987396487827908 2119 28-29 -0.2048214800518835 2119 30-31 -0.010131236608067695 2119 32-33 0.30476623190507723 2119 34-35 0.8790709856183767 2119 36-37 0.3141453243587904 2119 38-39 -1.809496838958161 2119 40-41 -2.471931548928815 2119 42-43 -0.5251878166692308 2119 44-45 0.829766959381061 2119 46-47 -0.2592758768866261 2119 48-49 -2.290841137750732 2119 50-51 0.29506478715682505 2119 52-53 0.15174935010470136 2119 54-55 0.4192390069219414 2119 56-57 -0.8018958920223298 2119 58-59 -0.39746069970793485 2119 60-61 -0.29555726654118786 2119 62-63 0.2480174414365095 2119 64-65 0.3053629345686417 2119 66-67 1.0390680205344438 2119 68-69 0.1635482205176828 2119 70-71 -0.6685222384569798 2119 72-73 0.5197733128272404 2119 74-75 -1.7744133336624408 2119 76 0.4122135234667539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 2.0 23 5.0 24 19.0 25 25.0 26 70.0 27 90.0 28 169.0 29 302.0 30 416.0 31 624.0 32 959.0 33 1256.0 34 1956.0 35 2638.0 36 3997.0 37 6809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.83204922695072 24.00330937483841 11.101918403226641 33.06272299498423 2 15.59025802781943 18.232587000361963 45.038523191478355 21.138631780340244 3 15.466156471379078 25.104710688246552 35.91188789492735 23.517244945447025 4 11.298412534257201 25.063343502766433 35.746419153006876 27.89182480996949 5 11.081234810486581 37.68033507420239 39.314338900667046 11.924091214643983 6 23.015667821500593 43.192512539428094 16.96054604684834 16.83127359222297 7 21.77982315528207 31.868245514245825 22.105589740938 24.2463415895341 8 20.109623041522312 40.07963183204923 20.704276332799008 19.106468793629453 9 20.71461812916904 14.204457314235484 19.65975489942603 45.42116965716945 10-11 21.392005791405968 27.28682972232277 29.347432649051143 21.973731837220125 12-13 22.17281141734319 20.244066394332698 28.455452712136097 29.12766947618801 14-15 19.73990382129376 22.739024768602306 21.544547287863903 35.976524122240036 16-17 24.08087284761363 28.551114328558867 21.642794353379184 25.72521847044832 18-19 24.9831945808987 26.26299188168985 22.596825068514402 26.156988468897048 20-21 21.640208904286677 23.76544805832773 22.379647344743784 32.21469569264181 22-23 21.371322198665908 29.370701690883706 23.08030404881328 26.177672061637107 24-25 24.062774703966078 30.738404260820108 22.15212782460313 23.046693210610684 26-27 26.44914421635038 30.725477015357566 22.819173690470034 20.006205077822017 28-29 26.219039247117227 24.51264284606236 22.860540875950154 26.40777703087026 30-31 24.323905062309322 29.613733905579398 23.181136563421067 22.881224468690213 32-33 21.588499922436526 28.46062361032111 24.9831945808987 24.967681886343655 34-35 23.93350224934071 25.83639278142613 25.31930296292466 24.910802006308497 36-37 21.32736956409328 26.728372718341177 29.481876001861522 22.46238171570402 38-39 20.443145974455764 24.45834841511971 28.416670975748488 26.681834634676044 40-41 19.14783597910957 24.026578416670976 31.19602875019391 25.62955685402554 42-43 24.83065308444077 22.475308961166554 30.99953461916335 21.694503335229328 44-45 18.625575262423084 26.630125652825896 30.205801747763587 24.538497336987437 46-47 24.727235120740474 22.299498422876056 27.493665649723358 25.47960080666012 48-49 22.8424427323026 22.25296033921092 26.870572418429077 28.0340245100574 50-51 18.11624179119913 22.583897823051863 31.98200527431615 27.317855111432856 52-53 18.289466880397125 22.886395366875227 36.69527896995708 22.128858782770568 54-55 15.884999224365274 22.948446145095403 33.19199544960959 27.974559180929724 56-57 22.27105848285847 22.604581415791923 27.700501577123948 27.423858524225658 58-59 16.88815347225813 26.335384456280053 33.31868245514246 23.457779616319353 60-61 21.301515073168208 31.532137132219866 25.347742902942237 21.818604891669683 62-63 16.678732095765035 37.395935674026575 23.52241584363204 22.40291638657635 64-65 16.285743833703915 38.08625058172604 22.454625368426495 23.173380216143542 66-67 16.882982574073115 38.20001034179637 21.707430580691867 23.209576503438647 68-69 17.05620766327111 36.964165675577846 22.079735250012927 23.899891411138114 70-71 17.699984487305446 35.986865918610064 21.267904234965613 25.04524535911888 72-73 18.12658358756916 32.94379233672889 22.100418842752987 26.82920523294896 74-75 18.760018615233463 32.351724494544705 22.012513573607738 26.8757433166141 76 18.749676818863435 33.915921195511665 20.64222555457883 26.69217643104607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.5 17 5.0 18 4.0 19 9.0 20 11.0 21 7.0 22 12.0 23 19.5 24 29.0 25 36.0 26 41.0 27 58.0 28 89.5 29 109.0 30 137.0 31 162.0 32 209.5 33 260.0 34 283.0 35 367.0 36 462.0 37 496.0 38 555.0 39 708.5 40 860.5 41 1145.0 42 1372.0 43 1574.5 44 1835.0 45 1761.5 46 1630.0 47 1561.0 48 1382.0 49 1141.0 50 1010.0 51 957.0 52 805.0 53 645.5 54 585.0 55 571.5 56 485.5 57 362.5 58 312.0 59 265.0 60 205.0 61 173.5 62 155.0 63 130.0 64 96.5 65 83.0 66 62.0 67 46.0 68 36.5 69 20.5 70 11.5 71 9.0 72 6.0 73 1.5 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 19339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.61678473550855 #Duplication Level Percentage of deduplicated Percentage of total 1 61.41246543288662 29.85676612027509 2 19.027866411401828 18.50147370598273 3 9.061901723037652 13.216815760897669 4 4.690491384811742 9.121464398365996 5 2.3824718145075514 5.791405967216505 6 1.3401403956604978 3.9091990278711415 7 0.7870665815783875 2.6785252598376337 8 0.4360774303339715 1.6960546046848337 9 0.2871729419272495 1.2565282589585811 >10 0.5211657094235269 3.629970525880345 >50 0.010636034886194426 0.2999120947308548 >100 0.021272069772388852 1.3082372408087284 >500 0.010636034886194426 2.714721547132737 >1k 0.010636034886194426 6.018925487357154 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 1164 6.018925487357154 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 525 2.714721547132737 TruSeq Adapter, Index 2 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 138 0.7135839495320337 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 115 0.5946532912766948 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT 58 0.2999120947308548 No Hit CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 48 0.2482031128807074 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCGT 40 0.20683592740058948 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 39 0.20166502921557475 No Hit TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT 36 0.18615233466053052 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 22 0.1137597600703242 No Hit CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 20 0.10341796370029474 No Hit ACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCT 20 0.10341796370029474 RNA PCR Primer, Index 2 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03619628729510316 0.0 2 0.0 0.0 0.0 0.04653808366513263 0.0 3 0.0 0.0 0.0 0.05687988003516211 0.0 4 0.0 0.0 0.0 0.09824706551528001 0.0 5 0.0 0.0 0.0 0.10858886188530947 0.0 6 0.0 0.0 0.0 0.10858886188530947 0.0 7 0.0 0.0 0.0 0.11893065825533895 0.0 8 0.0 0.0 0.0 0.14478514918041263 0.0 9 0.0 0.0 0.0 0.17581053829050106 0.0 10 0.0 0.0 0.0 0.20166502921557475 0.0 11 0.0 0.0 0.0 0.2533740110657221 0.0 12 0.0 0.0 0.0 0.28957029836082526 0.0 13 0.0 0.0 0.0 0.29474119654584 0.0 14 0.0 0.0 0.0 0.3050829929158695 0.0 15 0.0 0.0 0.0 0.3309374838409432 0.0 16 0.0 0.0 0.0 0.3361083820259579 0.0 17 0.0 0.0 0.0 0.3619628729510316 0.0 18 0.0 0.0 0.0 0.36713377113604634 0.0 19 0.0 0.0 0.0 0.38264646569109056 0.0 20 0.0 0.0 0.0 0.41367185480117896 0.0 21 0.0 0.0 0.0 0.4446972439112674 0.0 22 0.0 0.0 0.0 0.46538083665132635 0.0 23 0.0 0.0 0.0 0.46538083665132635 0.0 24 0.0 0.0 0.0 0.4860644293913853 0.0 25 0.0 0.0 0.0 0.5015771239464295 0.0 26 0.0 0.0 0.0 0.5067480221314442 0.0 27 0.0 0.0 0.0 0.5170898185014737 0.0 28 0.0 0.0 0.0 0.5636279021666063 0.0 29 0.0 0.0 0.0 0.5946532912766948 0.0 30 0.0 0.0 0.0 0.6256786803867832 0.0 31 0.0 0.0 0.0 0.6722167640519158 0.0 32 0.0 0.0 0.0 0.7135839495320337 0.0 33 0.0 0.0 0.0 0.8221728114173432 0.0 34 0.0 0.0 0.0 0.8997362841925642 0.0 35 0.0 0.0 0.0 1.142768498888257 0.0 36 0.0 0.0 0.0 1.3496044262888465 0.0 37 0.0 0.0 0.0 1.830497957495217 0.0 38 0.0 0.0 0.0 2.3269041832566315 0.0 39 0.0 0.0 0.0 3.0198045400486064 0.0 40 0.0 0.0 0.0 3.955737111536274 0.0 41 0.0 0.0 0.0 4.726200941103469 0.0 42 0.0 0.0 0.0 5.2277780650498995 0.0 43 0.0 0.0 0.0 5.9827292000620504 0.0 44 0.0 0.0 0.0 6.68080045503904 0.0 45 0.0 0.0 0.0 7.440922488236207 0.0 46 0.0 0.0 0.0 8.361342365168829 0.0 47 0.0 0.0 0.0 9.064584518330834 0.0 48 0.0 0.0 0.0 9.772997569677853 0.0 49 0.0 0.0 0.0 10.352138166399504 0.0 50 0.0 0.0 0.0 11.060551217746523 0.0 51 0.0 0.0 0.0 11.634520916283158 0.0 52 0.0 0.0 0.0 12.296395883965046 0.0 53 0.0 0.0 0.0 12.937587258906872 0.0 54 0.0 0.0 0.0 13.475360670148405 0.0 55 0.0 0.0 0.0 14.038988572315011 0.0 56 0.0 0.0 0.0 14.55090749263147 0.0 57 0.0 0.0 0.0 15.14038988572315 0.0 58 0.0 0.0 0.0 15.750555871554889 0.0 59 0.0 0.0 0.0 16.267645690056362 0.0 60 0.0 0.0 0.0 16.857128083148044 0.0 61 0.0 0.0 0.0 17.30182532705931 0.0 62 0.0 0.0 0.0 17.70515538549046 0.0 63 0.0 0.0 0.0 18.196390713066858 0.0 64 0.0 0.0 0.0 18.661771549718186 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATAA 15 0.002191817 70.00001 2 TCTACTG 20 7.73309E-5 69.99999 2 GTCTACT 20 7.73309E-5 69.99999 1 GTGTATA 20 0.0068494393 52.499996 1 TATAAGA 20 0.0068494393 52.499996 4 CGTCTTC 285 0.0 38.070175 48 CTGCTTG 280 0.0 37.5 54 TCTTCTG 280 0.0 37.5 50 CTTCTGC 280 0.0 37.5 51 CCGTCTT 290 0.0 37.41379 47 GCCGTCT 290 0.0 37.41379 46 TATGCCG 300 0.0 37.333332 43 GTCTTCT 285 0.0 36.842102 49 ATGCCGT 295 0.0 36.779663 44 CGTATGC 295 0.0 36.779663 41 TCGTATG 295 0.0 36.779663 40 CGTATCT 250 0.0 36.399998 34 TGAAAAA 260 0.0 36.346153 59 TTCTGCT 290 0.0 36.206898 52 GCACGTA 290 0.0 36.206898 31 >>END_MODULE