FastQCFastQC Report
Wed 25 May 2016
SRR1780711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165324
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAGC351.431763E-744.01666638
CGAGTCT254.4242603E-544.0033324
TGGCATG207.8456564E-443.99000527
TAACCGG254.4321838E-543.99000522
TTCCGAC254.440119E-543.9766942
TGTATAG207.8925985E-443.9367833
ACTAGCC404.0838677E-738.51458439
TTTGCCC404.1130988E-738.47960332
ACTTCGA357.158609E-637.78582420
TACCCGC357.248611E-637.7171411
TTGCCCA357.2788243E-637.694333
CAGAGTA301.2841809E-436.7250714
TACTTCG301.2864766E-436.71393619
GTACTTC301.2864766E-436.71393618
CTACCCG301.295694E-436.66944510
GAGTCTC301.2980067E-436.6583425
GGCCATT301.3026426E-436.63614744
GCGGCTA250.002325201435.26676620
GCCATTT250.002332137735.24537313
GTCCCGA250.002332137735.24537318
Produced by FastQC (version 0.11.5)