FastQCFastQC Report
Wed 25 May 2016
SRR1780711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165324
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT147858.943045171904867No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT54703.308654520819724RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC24071.4559289637318236No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG13340.806900389538119No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC4780.2891292250368972RNA PCR Primer, Index 34 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT4190.2534417265490794RNA PCR Primer, Index 34 (95% over 22bp)
ATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCTGCTT3040.18388134814062085Illumina PCR Primer Index 3 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC3000.18146185671771795No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC2570.1554523239215117No Hit
CATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCTGCT2390.1445646125184486RNA PCR Primer, Index 34 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCT2330.14093537538409426RNA PCR Primer, Index 34 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT207.8374427E-443.99939734
GACACGT207.8374427E-443.99939710
CCGGATA207.8374427E-443.9993974
GAATCGG207.8374427E-443.99939720
CGACCAA207.8374427E-443.99939712
GGATATC207.8374427E-443.99939735
ATCGTAT207.8374427E-443.99939739
AGCGGAA207.8374427E-443.99939716
ATATCGT207.8374427E-443.99939737
CGTAGCG207.8374427E-443.99939713
GGTATGC207.8374427E-443.99939728
CATCGTT207.8374427E-443.99939732
AGTTAGA207.8374427E-443.99939736
ACGTACT207.8374427E-443.99939713
GGACCGT207.8374427E-443.99939739
CTTTATA207.8374427E-443.9993972
CACGTAC207.8374427E-443.99939712
CGGAGCT207.8374427E-443.99939742
TAATACG404.0965097E-738.4994744
AGCGGAT404.0965097E-738.49947435
Produced by FastQC (version 0.11.5)