##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780710_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3048 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.969160104986877 23.0 12.0 32.0 12.0 34.0 2 20.386154855643046 21.0 11.0 31.0 11.0 34.0 3 21.003937007874015 21.0 11.0 32.0 11.0 34.0 4 21.761154855643046 23.0 11.0 32.0 11.0 34.0 5 22.01509186351706 23.0 11.0 32.0 11.0 34.0 6 23.43241469816273 26.0 11.0 34.0 11.0 37.0 7 22.120406824146983 21.0 11.0 34.0 11.0 37.0 8 21.72309711286089 21.0 11.0 33.0 11.0 37.0 9 22.621062992125985 21.0 12.0 34.0 12.0 37.0 10-11 21.93520341207349 21.0 11.0 34.0 11.0 37.0 12-13 21.9507874015748 21.5 11.0 33.5 11.0 37.0 14-15 21.651410761154857 21.0 11.0 33.5 11.0 37.0 16-17 21.01755249343832 21.0 11.0 31.0 11.0 37.0 18-19 21.47309711286089 21.0 11.0 32.5 11.0 37.0 20-21 21.681102362204726 21.5 11.0 32.5 11.0 37.0 22-23 21.0871062992126 21.0 11.0 31.0 11.0 37.0 24-25 21.284284776902886 21.0 11.0 31.5 11.0 37.0 26-27 21.008858267716537 21.0 11.0 31.0 11.0 37.0 28-29 20.38992782152231 21.0 11.0 31.0 11.0 37.0 30-31 20.383530183727032 21.0 11.0 30.5 11.0 37.0 32-33 20.69570209973753 21.0 11.0 31.0 11.0 37.0 34-35 20.88533464566929 21.0 11.0 31.0 11.0 36.0 36-37 20.59022309711286 21.0 11.0 29.0 11.0 36.5 38-39 19.826443569553806 11.0 11.0 27.0 11.0 36.0 40-41 20.330216535433074 21.0 11.0 27.0 11.0 36.0 42-43 20.55725065616798 16.0 11.0 29.0 11.0 36.0 44-45 20.37516404199475 21.0 11.0 27.0 11.0 36.0 46-47 20.541010498687662 21.0 11.0 27.0 11.0 36.0 48-49 20.585629921259844 21.0 11.0 29.0 11.0 36.0 50-51 20.684055118110237 21.0 11.0 29.5 11.0 36.0 52-53 20.819225721784775 21.0 11.0 31.0 11.0 36.0 54-55 20.913385826771652 21.0 11.0 31.0 11.0 36.0 56-57 20.418799212598422 16.0 11.0 29.0 11.0 36.0 58-59 20.058398950131235 16.0 11.0 29.0 11.0 36.0 60-61 20.517224409448822 21.0 11.0 29.0 11.0 36.0 62-63 19.98244750656168 15.5 11.0 27.0 11.0 36.0 64-65 20.07086614173228 15.5 11.0 27.0 11.0 36.0 66-67 20.538385826771652 20.0 11.0 29.0 11.0 36.0 68-69 19.75 19.0 11.0 27.0 11.0 35.5 70-71 19.550688976377952 15.0 11.0 26.5 11.0 34.5 72-73 18.97818241469816 11.0 11.0 25.5 11.0 34.0 74-75 19.476870078740156 19.0 11.0 26.0 11.0 34.0 76 18.686023622047244 11.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -3.9718588429114803 1101 2 -5.261017053122316 1101 3 -0.9869370395686197 1101 4 -4.427918499629023 1101 5 -1.0803383750752182 1101 6 0.0979806742964584 1101 7 -2.5727475740633636 1101 8 -3.4507914600019944 1101 9 -0.27382734224839567 1101 10-11 0.022194326141690368 1101 12-13 -3.195545171203065 1101 14-15 0.7700903263403269 1101 16-17 0.7414157114815083 1101 18-19 1.6294808992177394 1101 20-21 -2.214544592834063 1101 22-23 0.42061388903493935 1101 24-25 -2.5998229475203125 1101 26-27 -1.4940586460323289 1101 28-29 -2.8880806328174735 1101 30-31 -0.15244864784338574 1101 32-33 -0.12982233409864818 1101 34-35 0.7726006084558676 1101 36-37 -5.149148110661265 1101 38-39 -0.4328616558879794 1101 40-41 0.9628693674746351 1101 42-43 -2.3684988987620628 1101 44-45 0.2379937679279749 1101 46-47 -1.8085780447622497 1101 48-49 0.631417632075518 1101 50-51 0.6566164829322751 1101 52-53 -0.9428238428238451 1101 54-55 -2.7786500779921823 1101 56-57 0.024369891804099097 1101 58-59 -3.360287921143179 1101 60-61 -5.543977878846299 1101 62-63 -1.0552937194384562 1101 64-65 -0.8391679592337518 1101 66-67 -2.5292572193888 1101 68-69 -1.6724582800240668 1101 70-71 -2.499630449959394 1101 72-73 -4.013603903406533 1101 74-75 -1.421642502234608 1101 76 -2.8136078833447247 1102 1 2.1392522681996304 1102 2 4.294538502433237 1102 3 1.9019518493202696 1102 4 -1.0945851662956905 1102 5 0.2529949582581139 1102 6 7.209091785407569 1102 7 3.8716968703810792 1102 8 4.882541873331341 1102 9 1.281728213307158 1102 10-11 1.577749881697244 1102 12-13 2.3600103843524884 1102 14-15 3.158979215229216 1102 16-17 3.9636379337037297 1102 18-19 4.407258676995518 1102 20-21 4.063233184943712 1102 22-23 3.0872805557016036 1102 24-25 4.511288163590798 1102 26-27 -0.49405864603232885 1102 28-29 5.500808256071416 1102 30-31 3.403106907712168 1102 32-33 4.092399888123573 1102 34-35 6.494822830678089 1102 36-37 2.7952963337831775 1102 38-39 5.233805010778688 1102 40-41 4.907313811919078 1102 42-43 7.07594554568238 1102 44-45 4.182438212372421 1102 46-47 5.0247552885710824 1102 48-49 7.020306520964411 1102 50-51 1.4899498162656073 1102 52-53 4.501620601620598 1102 54-55 4.110238810896703 1102 56-57 3.024369891804099 1102 58-59 5.02860096774571 1102 60-61 3.178244343375926 1102 62-63 1.333595169450433 1102 64-65 5.99416537409958 1102 66-67 5.304076113944532 1102 68-69 -0.22801383557962396 1102 70-71 3.1114806611517167 1102 72-73 4.486396096593467 1102 74-75 -0.8660869466790544 1102 76 2.297503227766388 1103 1 -2.460747731800371 1103 2 -1.6499059420112054 1103 3 -3.1536037062352875 1103 4 -0.8945851662956912 1103 5 4.441883847147004 1103 6 1.686869563185347 1103 7 0.1494746481588578 1103 8 0.1047640955535627 1103 9 0.13728376886271576 1103 10-11 -1.2666945627471975 1103 12-13 2.5433437176858256 1103 14-15 -4.007687451437452 1103 16-17 -3.064139844074049 1103 18-19 -3.1205191007822606 1103 20-21 -0.09787792616739566 1103 22-23 -2.351608333187283 1103 24-25 -5.438711836409203 1103 26-27 -3.294058646032326 1103 28-29 -4.349191743928584 1103 30-31 -1.9913375367322779 1103 32-33 -3.1131556674319825 1103 34-35 -2.527399391544133 1103 36-37 -1.671370332883484 1103 38-39 -3.477306100332422 1103 40-41 -1.4149084103031448 1103 42-43 -5.207387787650951 1103 44-45 -2.9453395654053587 1103 46-47 -3.041911378095584 1103 48-49 0.31475096540885517 1103 50-51 0.5510609273767209 1103 52-53 -2.7150460650460673 1103 54-55 -3.184205633547741 1103 56-57 -3.4256301081959 1103 58-59 -3.6269545878098484 1103 60-61 -3.305088989957408 1103 62-63 -3.355293719438457 1103 64-65 -1.3280568481226425 1103 66-67 -1.6403683304999106 1103 68-69 -1.350236057801844 1103 70-71 -1.3662971166260611 1103 72-73 -0.7858261256287555 1103 74-75 1.4950241644320563 1103 76 -4.36916343890028 1104 1 -3.082969954022591 1104 2 -0.5943503864556483 1104 3 -0.9869370395686197 1104 4 -4.872362944073469 1104 5 -2.3025605972974397 1104 6 -5.790908214592431 1104 7 -8.572747574063364 1104 8 -1.2285692377797695 1104 9 3.392839324418272 1104 10-11 -4.533361229413867 1104 12-13 -4.362211837869733 1104 14-15 -2.674354118104116 1104 16-17 -2.703028732962938 1104 18-19 -0.4816302118933713 1104 20-21 -2.825655703945177 1104 22-23 -2.190497222076168 1104 24-25 -3.488711836409202 1104 26-27 -5.60516975714344 1104 28-29 -0.2769695217063628 1104 30-31 1.4031069077121678 1104 32-33 -1.4631556674319839 1104 34-35 -3.6162882804330216 1104 36-37 0.01751855600540253 1104 38-39 -0.488417211443533 1104 40-41 0.407313811919078 1104 42-43 -2.312943343206509 1104 44-45 -2.9842284542942465 1104 46-47 -5.086355822540028 1104 48-49 -5.368582367924478 1104 50-51 -0.8433835170677249 1104 52-53 -5.887268287268288 1104 54-55 0.38801658867448197 1104 56-57 -1.9200745526403438 1104 58-59 -2.1936212544765112 1104 60-61 0.3449110100425905 1104 62-63 -3.721960386105124 1104 64-65 1.1608320407662482 1104 66-67 -3.6403683304999106 1104 68-69 -1.8946805022462883 1104 70-71 -1.1662971166260618 1104 72-73 -3.6802705700731995 1104 74-75 2.2450241644320563 1104 76 -1.1469412166780586 1105 1 1.446944575891937 1105 2 1.0808632887580245 1105 3 0.3463962937647125 1105 4 1.2131071413966161 1105 5 -1.5504238451606902 1105 6 1.7561003324161177 1105 7 -1.5043715056872955 1105 8 2.027841018630486 1105 9 -0.47040853882959155 1105 10-11 1.9794592834066478 1105 12-13 2.3087283330704373 1105 14-15 1.0307740870240885 1105 16-17 1.147398617464411 1105 18-19 -2.3705191007822606 1105 20-21 3.375198996909525 1105 22-23 1.236853205274258 1105 24-25 0.5112881635907982 1105 26-27 1.1597875078138244 1105 28-29 0.15465440991756907 1105 30-31 -0.4644144598091984 1105 32-33 1.0753058710295562 1105 34-35 -1.4966301607748989 1105 36-37 3.1670912055780533 1105 38-39 -2.838844561870882 1105 40-41 1.4158608204660865 1105 42-43 1.742612212349048 1105 44-45 -1.2491857192515106 1105 46-47 1.550396314212108 1105 48-49 2.1955201961780837 1105 50-51 2.216445542761331 1105 52-53 2.565723165723167 1105 54-55 2.6273328279907204 1105 56-57 0.3320621994964057 1105 58-59 -0.6808007416560002 1105 60-61 1.7295263946579773 1105 62-63 -2.632216796361533 1105 64-65 -1.1126722327380278 1105 66-67 -4.294214484346064 1105 68-69 -1.4117745193403053 1105 70-71 -2.8586048089337535 1105 72-73 2.341096951294322 1105 74-75 -0.2934373740294838 1105 76 -1.36916343890028 1106 1 -0.1940810651337017 1106 2 0.2945385024332374 1106 3 0.013062960431380333 1106 4 1.2387481670376417 1106 5 -2.3025605972974397 1106 6 0.7646473409631263 1106 7 -2.6838586851744743 1106 8 0.1047640955535627 1106 9 1.281728213307158 1106 10-11 -2.0889167849694203 1106 12-13 -0.41776739342528657 1106 14-15 -1.7854652292152302 1106 16-17 2.5191934892592833 1106 18-19 3.6294808992177394 1106 20-21 0.3410109627214908 1106 22-23 -2.246052777631725 1106 24-25 -1.7664896141869804 1106 26-27 3.6170524650787854 1106 28-29 0.27858603384919434 1106 30-31 1.1808846854899464 1106 32-33 0.4257332214569054 1106 34-35 -0.5607327248774645 1106 36-37 4.350851889338735 1106 38-39 -1.5995283225546437 1106 40-41 -1.2037972991920327 1106 42-43 -0.8129433432065092 1106 44-45 0.46021599015019987 1106 46-47 1.2469775107933039 1106 48-49 -0.3130268123689248 1106 50-51 2.7121720384878287 1106 52-53 1.3349539349539334 1106 54-55 3.3324610331189284 1106 56-57 0.024369891804099097 1106 58-59 0.7508231899679316 1106 60-61 4.789355454487033 1106 62-63 4.000261836117101 1106 64-65 4.105276485210691 1106 66-67 -0.3070349971665749 1106 68-69 6.271986164420376 1106 70-71 -0.5551860055149476 1106 72-73 -0.6802705700731977 1106 74-75 3.4672463866542778 1106 76 4.519725449988609 1107 1 -0.5274143984670374 1107 2 -1.7332392753445376 1107 3 -0.4036037062352875 1107 4 -0.8445851662956905 1107 5 1.808550513813671 1107 6 3.82020289651868 1107 7 5.48280798149219 1107 8 4.0214307622202305 1107 9 2.587283768862715 1107 10-11 1.0916387705861332 1107 12-13 -1.2233229489808437 1107 14-15 -1.2993541181041195 1107 16-17 2.560860155925951 1107 18-19 0.9211475658844037 1107 20-21 1.3687887404992658 1107 22-23 1.198391666812718 1107 24-25 0.8029548302574625 1107 26-27 4.214274687301007 1107 28-29 0.9174749227380801 1107 30-31 1.333662463267725 1107 32-33 0.7868443325680161 1107 34-35 2.2309339417892 1107 36-37 0.6702963337831811 1107 38-39 3.2476938996675777 1107 40-41 3.1850915896968566 1107 42-43 -2.340721120984284 1107 44-45 0.5296604345946427 1107 46-47 1.7330886219044146 1107 48-49 0.42308429874218945 1107 50-51 2.101060927376718 1107 52-53 2.0016206016205977 1107 54-55 -0.08420563354773947 1107 56-57 2.524369891804099 1107 58-59 -0.31862125447651124 1107 60-61 -0.1550889899574095 1107 62-63 -0.6802937194384562 1107 64-65 -0.853056848122641 1107 66-67 -4.848701663833243 1107 68-69 -1.3669027244685097 1107 70-71 0.33370288337393816 1107 72-73 1.8891738743712452 1107 74-75 5.453357497765392 1107 76 3.38083656109972 1108 1 4.13925226819963 1108 2 6.122821330716068 1108 3 -1.471785524417104 1108 4 0.6326875609770362 1108 5 4.141883847147003 1108 6 3.168687745003531 1108 7 1.3767473754315844 1108 8 5.286582277371746 1108 9 7.473647405226352 1108 10-11 3.9060327099800745 1108 12-13 6.602434626776731 1108 14-15 2.7650398212898217 1108 16-17 0.04949651956231449 1108 18-19 0.6749354446722862 1108 20-21 6.406667528378055 1108 22-23 3.7438462122672647 1108 24-25 5.193106345408978 1108 26-27 3.9150322630585777 1108 28-29 4.228080983344142 1108 30-31 -0.041337536732275026 1108 32-33 2.8095716052952895 1108 34-35 2.9544187902740475 1108 36-37 0.44681148529833337 1108 38-39 -3.4682151912415122 1108 40-41 -1.7239993193940535 1108 42-43 0.7880667578035947 1108 44-45 0.7228422527764629 1108 46-47 -0.3237295599137653 1108 48-49 -1.8534308527729664 1108 50-51 -0.4898481635323755 1108 52-53 -0.9832278832278867 1108 54-55 -0.6978419971841028 1108 56-57 -1.475630108195901 1108 58-59 -5.117863678718939 1108 60-61 1.3903655554971373 1108 62-63 -0.6007482648930029 1108 64-65 -0.6826023026680943 1108 66-67 -0.1403683304999106 1108 68-69 -0.2684178759836655 1108 70-71 2.1518847015557583 1108 72-73 0.8096284198257919 1108 74-75 1.1541150735229628 1108 76 2.0853820156451732 1109 1 -4.460747731800371 1109 2 2.050094057988794 1109 3 -1.6536037062352875 1109 4 2.505414833704311 1109 5 4.041883847147002 1109 6 -2.1131304368146537 1109 7 0.3494746481588571 1109 8 -4.995235904446437 1109 9 -1.9627162311372857 1109 10-11 1.633305437252801 1109 12-13 2.393343717685827 1109 14-15 -0.007687451437451642 1109 16-17 -3.764139844074048 1109 18-19 1.029480899217738 1109 20-21 -3.3978779261674 1109 22-23 -1.9016083331872835 1109 24-25 0.6612881635907968 1109 26-27 0.5059413539676711 1109 28-29 0.05080825607141648 1109 30-31 2.0586624632677264 1109 32-33 0.2868443325680161 1109 34-35 0.022600608455867643 1109 36-37 1.4786296671165147 1109 38-39 -0.6273061003324223 1109 40-41 2.1850915896968566 1109 42-43 -0.20738778765095134 1109 44-45 1.1546604345946427 1109 46-47 1.7580886219044132 1109 48-49 -2.835249034591147 1109 50-51 -0.048939072623280566 1109 52-53 1.3349539349539334 1109 54-55 0.3657943664522634 1109 56-57 -1.0256301081959016 1109 58-59 0.8730454121901516 1109 60-61 1.1449110100425877 1109 62-63 2.144706280561543 1109 64-65 -1.228056848122641 1109 66-67 -2.3903683304999106 1109 68-69 -2.4502360578018454 1109 70-71 -1.9162971166260618 1109 72-73 -0.6858261256287541 1109 74-75 0.8450241644320577 1109 76 -0.46916343890028145 1110 1 2.8392522681996297 1110 2 0.950094057988796 1110 3 2.246396293764711 1110 4 4.70541483370431 1110 5 5.441883847147004 1110 6 2.3868695631853463 1110 7 2.0494746481588564 1110 8 3.0047640955535613 1110 9 8.437283768862716 1110 10-11 2.883305437252801 1110 12-13 6.593343717685823 1110 14-15 6.492312548562548 1110 16-17 5.08586015592595 1110 18-19 2.8794808992177394 1110 20-21 8.8521220738326 1110 22-23 5.798391666812719 1110 24-25 1.5112881635907982 1110 26-27 5.90594135396767 1110 28-29 2.850808256071417 1110 30-31 3.3086624632677264 1110 32-33 6.8868443325680175 1110 34-35 2.222600608455867 1110 36-37 0.2786296671165118 1110 38-39 1.2226938996675791 1110 40-41 2.6850915896968566 1110 42-43 5.742612212349048 1110 44-45 4.604660434594646 1110 46-47 0.8080886219044174 1110 48-49 3.8647509654088523 1110 50-51 3.1510609273767187 1110 52-53 1.7849539349539327 1110 54-55 -0.08420563354773947 1110 56-57 3.8243698918041 1110 58-59 2.9230454121901523 1110 60-61 2.0949110100425905 1110 62-63 0.9447062805615438 1110 64-65 1.021943151877359 1110 66-67 5.459631669500091 1110 68-69 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2117 7 -3.5505253518411415 2117 8 8.104764095553563 2117 9 4.837283768862715 2117 10-11 2.2333054372528025 2117 12-13 3.693343717685824 2117 14-15 4.792312548562549 2117 16-17 2.0858601559259498 2117 18-19 6.529480899217738 2117 20-21 4.052122073832603 2117 22-23 5.398391666812717 2117 24-25 3.511288163590798 2117 26-27 2.805941353967672 2117 28-29 3.8008082560714165 2117 30-31 -3.541337536732275 2117 32-33 0.9368443325680147 2117 34-35 -1.3273993915441338 2117 36-37 5.428629667116514 2117 38-39 3.822693899667577 2117 40-41 -2.414908410303145 2117 42-43 4.342612212349049 2117 44-45 2.3046604345946413 2117 46-47 -0.34191137809558825 2117 48-49 5.164750965408853 2117 50-51 -5.498939072623282 2117 52-53 -3.4650460650460637 2117 54-55 -2.0342056335477388 2117 56-57 6.424369891804098 2117 58-59 1.473045412190153 2117 60-61 3.244911010042589 2117 62-63 -1.5552937194384562 2117 64-65 8.171943151877358 2117 66-67 5.259631669500088 2117 68-69 3.649763942198156 2117 70-71 8.833702883373938 2117 72-73 -2.1358261256287534 2117 74-75 0.045024164432057034 2117 76 2.8308365610997193 2118 1 -1.6607477318003703 2118 2 -4.949905942011206 2118 3 -3.6536037062352875 2118 4 -0.29458516629568976 2118 5 -0.4581161528529982 2118 6 -1.413130436814651 2118 7 1.0494746481588564 2118 8 2.1047640955535627 2118 9 -3.9627162311372857 2118 10-11 3.933305437252802 2118 12-13 0.9933437176858213 2118 14-15 -0.20768745143745093 2118 16-17 0.4858601559259519 2118 18-19 -7.670519100782261 2118 20-21 -3.8478779261673957 2118 22-23 -2.601608333187283 2118 24-25 2.1112881635907996 2118 26-27 -0.7940586460323296 2118 28-29 1.8008082560714165 2118 30-31 0.6586624632677278 2118 32-33 -3.163155667431983 2118 34-35 -0.7273993915441324 2118 36-37 -7.771370332883487 2118 38-39 1.822693899667577 2118 40-41 -2.414908410303145 2118 42-43 -2.6573877876509506 2118 44-45 2.1046604345946456 2118 46-47 -4.841911378095585 2118 48-49 -2.6352490345911477 2118 50-51 -3.4989390726232834 2118 52-53 -3.365046065046066 2118 54-55 -1.5342056335477388 2118 56-57 -5.275630108195902 2118 58-59 -4.226954587809846 2118 60-61 -4.1550889899574095 2118 62-63 -1.2552937194384555 2118 64-65 -1.5280568481226382 2118 66-67 -1.9403683304999113 2118 68-69 -3.350236057801844 2118 70-71 -0.7662971166260633 2118 72-73 1.1641738743712438 2118 74-75 -0.6549758355679423 2118 76 0.8308365610997193 2119 1 -5.36074773180037 2119 2 5.850094057988795 2119 3 -3.6536037062352875 2119 4 -11.09458516629569 2119 5 -10.858116152852997 2119 6 -12.513130436814652 2119 7 4.649474648158858 2119 8 -2.3952359044464373 2119 9 -10.162716231137285 2119 10-11 -7.866694562747199 2119 12-13 -6.056656282314176 2119 14-15 1.4923125485625484 2119 16-17 -8.814139844074049 2119 18-19 1.6294808992177394 2119 20-21 -1.2978779261673985 2119 22-23 -6.301608333187282 2119 24-25 -1.4887118364092018 2119 26-27 -2.244058646032329 2119 28-29 -5.499191743928584 2119 30-31 -2.291337536732275 2119 32-33 -4.713155667431984 2119 34-35 1.0226006084558676 2119 36-37 -3.6213703328834868 2119 38-39 -2.8773061003324223 2119 40-41 -4.314908410303143 2119 42-43 -2.257387787650952 2119 44-45 -3.0953395654053573 2119 46-47 7.108088621904415 2119 48-49 -2.2852490345911463 2119 50-51 -8.898939072623282 2119 52-53 -2.9150460650460666 2119 54-55 -3.5842056335477395 2119 56-57 -2.475630108195901 2119 58-59 -4.026954587809847 2119 60-61 -1.6550889899574095 2119 62-63 -6.055293719438456 2119 64-65 -5.478056848122641 2119 66-67 0.3596316695000894 2119 68-69 -2.9502360578018454 2119 70-71 1.5837028833739382 2119 72-73 5.264173874371245 2119 74-75 -2.0049758355679437 2119 76 -1.36916343890028 >>END_MODULE >>Per sequence quality scores fail #Quality Count 10 1.0 11 0.0 12 6.0 13 42.0 14 134.0 15 205.0 16 318.0 17 351.0 18 341.0 19 342.0 20 288.0 21 223.0 22 168.0 23 112.0 24 64.0 25 63.0 26 43.0 27 29.0 28 20.0 29 23.0 30 15.0 31 23.0 32 18.0 33 29.0 34 29.0 35 44.0 36 53.0 37 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.360872438863186 27.42894910773298 8.19563780568407 56.014540647719755 2 4.396325459317586 22.867454068241468 42.45406824146982 30.28215223097113 3 4.035433070866142 46.03018372703412 23.16272965879265 26.77165354330709 4 3.937007874015748 26.443569553805773 20.24278215223097 49.376640419947506 5 3.5761154855643045 29.92125984251969 19.914698162729657 46.58792650918635 6 6.13517060367454 47.60498687664042 9.973753280839896 36.28608923884514 7 6.36482939632546 36.9750656167979 22.211286089238847 34.44881889763779 8 5.118110236220472 35.20341207349082 25.853018372703414 33.8254593175853 9 9.547244094488189 28.41207349081365 16.50262467191601 45.53805774278215 10-11 6.9717847769028864 29.87204724409449 27.6738845144357 35.482283464566926 12-13 7.463910761154856 26.033464566929133 26.181102362204722 40.32152230971129 14-15 11.122047244094487 31.020341207349084 21.24343832020997 36.61417322834646 16-17 14.81299212598425 30.52821522309711 19.71784776902887 34.94094488188976 18-19 12.38517060367454 28.03477690288714 17.61811023622047 41.96194225721785 20-21 10.301837270341208 27.919947506561684 19.89829396325459 41.87992125984252 22-23 17.55249343832021 29.6259842519685 15.961286089238847 36.860236220472444 24-25 13.270997375328083 34.05511811023622 16.715879265091864 35.958005249343834 26-27 10.974409448818898 35.61351706036746 20.078740157480315 33.33333333333333 28-29 14.156824146981627 29.90485564304462 24.229002624671917 31.70931758530184 30-31 13.664698162729657 28.526902887139105 26.673228346456696 31.13517060367454 32-33 10.28543307086614 35.039370078740156 23.310367454068242 31.36482939632546 34-35 9.416010498687664 36.5485564304462 21.555118110236222 32.48031496062992 36-37 12.23753280839895 29.44553805774278 23.786089238845147 34.53083989501312 38-39 13.254593175853019 24.29461942257218 27.017716535433074 35.43307086614173 40-41 9.744094488188976 27.95275590551181 26.87007874015748 35.43307086614173 42-43 6.13517060367454 25.787401574803148 28.28083989501312 39.79658792650918 44-45 7.578740157480316 24.29461942257218 33.218503937007874 34.90813648293963 46-47 8.103674540682416 30.05249343832021 27.526246719160103 34.31758530183727 48-49 7.2998687664042 34.104330708661415 21.358267716535433 37.237532808398946 50-51 7.742782152230972 31.758530183727036 20.93175853018373 39.56692913385827 52-53 6.020341207349081 33.234908136482936 22.178477690288716 38.566272965879264 54-55 6.118766404199475 30.511811023622048 21.14501312335958 42.2244094488189 56-57 10.810367454068242 31.660104986876643 20.02952755905512 37.5 58-59 10.25262467191601 30.396981627296586 19.438976377952756 39.911417322834644 60-61 7.759186351706036 27.739501312335957 21.128608923884514 43.37270341207349 62-63 9.711286089238845 29.051837270341206 18.503937007874015 42.73293963254593 64-65 11.745406824146981 29.970472440944885 15.38713910761155 42.89698162729659 66-67 8.185695538057743 35.892388451443566 14.534120734908138 41.38779527559055 68-69 12.713254593175852 35.18700787401575 12.992125984251967 39.107611548556434 70-71 12.795275590551181 36.08923884514436 16.289370078740156 34.8261154855643 72-73 16.601049868766403 33.743438320209975 14.419291338582676 35.23622047244094 74-75 11.269685039370078 38.270997375328086 13.910761154855644 36.5485564304462 76 10.990813648293964 35.43307086614173 18.471128608923884 35.10498687664042 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.5 14 1.5 15 1.5 16 0.5 17 0.0 18 2.5 19 4.0 20 3.5 21 4.0 22 3.0 23 3.5 24 5.5 25 6.0 26 10.5 27 11.5 28 13.0 29 18.0 30 23.0 31 29.5 32 32.0 33 33.0 34 50.0 35 68.5 36 80.0 37 90.0 38 84.5 39 95.0 40 125.5 41 141.5 42 143.0 43 156.5 44 182.0 45 189.5 46 185.0 47 197.0 48 195.5 49 176.5 50 171.0 51 177.0 52 160.5 53 135.0 54 132.0 55 129.5 56 113.0 57 94.0 58 89.0 59 77.5 60 62.0 61 50.5 62 43.0 63 39.5 64 29.5 65 22.0 66 17.5 67 14.0 68 11.0 69 7.0 70 4.0 71 2.0 72 3.5 73 5.5 74 3.5 75 1.0 76 1.0 77 1.0 78 2.0 79 3.0 80 1.5 81 0.5 82 1.5 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7217847769028871 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 3048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.80183727034121 #Duplication Level Percentage of deduplicated Percentage of total 1 98.38980207983899 96.22703412073491 2 1.107011070110701 2.1653543307086616 3 0.36900369003690037 1.0826771653543308 4 0.13418316001341832 0.5249343832020997 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCCTCTCAGACTGCCCTATAAGAAATACTAAAGGGAGTCCAACAGCTG 4 0.13123359580052493 No Hit GGCTGAATGCATTAATCATCCTGGACTGGGTTGTGCTGCAGCTGTCTCTT 4 0.13123359580052493 No Hit GTATACTTTTAAGTAATAGCAACTCCCATCAATAGTAAATCAGGACTGTC 4 0.13123359580052493 No Hit ACCAGAGACCCACGGTCGGGCCGATAGCTCTCGGAGCGGTAATCATCCTG 4 0.13123359580052493 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0984251968503937 0.0 2 0.0 0.0 0.0 0.0984251968503937 0.0 3 0.0 0.0 0.0 0.0984251968503937 0.0 4 0.0 0.0 0.0 0.0984251968503937 0.0 5 0.0 0.0 0.0 0.0984251968503937 0.0 6 0.0 0.0 0.0 0.0984251968503937 0.0 7 0.0 0.0 0.0 0.0984251968503937 0.0 8 0.0 0.0 0.0 0.0984251968503937 0.0 9 0.0 0.0 0.0 0.0984251968503937 0.0 10 0.0 0.0 0.0 0.16404199475065617 0.0 11 0.0 0.0 0.0 0.16404199475065617 0.0 12 0.0 0.0 0.0 0.16404199475065617 0.0 13 0.0 0.0 0.0 0.16404199475065617 0.0 14 0.0 0.0 0.0 0.1968503937007874 0.0 15 0.0 0.0 0.0 0.1968503937007874 0.0 16 0.0 0.0 0.0 0.1968503937007874 0.0 17 0.0 0.0 0.0 0.1968503937007874 0.0 18 0.0 0.0 0.0 0.2952755905511811 0.0 19 0.0 0.0 0.0 0.42650918635170604 0.0 20 0.0 0.0 0.0 0.5249343832020997 0.0 21 0.0 0.0 0.0 0.5249343832020997 0.0 22 0.0 0.0 0.0 0.5577427821522309 0.0 23 0.0 0.0 0.0 0.5905511811023622 0.0 24 0.0 0.0 0.0 0.7217847769028871 0.0 25 0.0 0.0 0.0 0.7217847769028871 0.0 26 0.0 0.0 0.0 0.7217847769028871 0.0 27 0.0 0.0 0.0 0.7217847769028871 0.0 28 0.0 0.0 0.0 0.7217847769028871 0.0 29 0.0 0.0 0.0 0.7545931758530183 0.0 30 0.0 0.0 0.0 0.7545931758530183 0.0 31 0.0 0.0 0.0 0.7545931758530183 0.0 32 0.0 0.0 0.0 0.7874015748031497 0.0 33 0.0 0.0 0.0 0.8530183727034121 0.0 34 0.0 0.0 0.0 0.9514435695538058 0.0 35 0.0 0.0 0.0 1.0170603674540681 0.0 36 0.0 0.0 0.0 1.0826771653543308 0.0 37 0.0 0.0 0.0 1.279527559055118 0.0 38 0.0 0.0 0.0 1.3779527559055118 0.0 39 0.0 0.0 0.0 2.0013123359580054 0.0 40 0.0 0.0 0.0 2.3622047244094486 0.0 41 0.0 0.0 0.0 2.559055118110236 0.0 42 0.0 0.0 0.0 3.510498687664042 0.0 43 0.0 0.0 0.0 3.7401574803149606 0.0 44 0.0 0.0 0.0 4.002624671916011 0.0 45 0.0 0.0 0.0 4.396325459317585 0.0 46 0.0 0.0 0.0 4.691601049868766 0.0 47 0.0 0.0 0.0 4.822834645669292 0.0 48 0.0 0.0 0.0 5.183727034120735 0.0 49 0.0 0.0 0.0 5.347769028871391 0.0 50 0.0 0.0 0.0 5.610236220472441 0.0 51 0.0 0.0 0.0 5.905511811023622 0.0 52 0.0 0.0 0.0 5.938320209973753 0.0 53 0.0 0.0 0.0 6.036745406824147 0.0 54 0.0 0.0 0.0 6.233595800524935 0.0 55 0.0 0.0 0.0 6.758530183727034 0.0 56 0.0 0.0 0.0 6.92257217847769 0.0 57 0.0 0.0 0.0 6.955380577427822 0.0 58 0.0 0.0 0.0 7.086614173228346 0.0 59 0.0 0.0 0.0 7.086614173228346 0.0 60 0.0 0.0 0.0 7.283464566929134 0.0 61 0.0 0.0 0.0 7.283464566929134 0.0 62 0.0 0.0 0.0 7.513123359580052 0.0 63 0.0 0.0 0.0 7.677165354330708 0.0 64 0.0 0.0 0.0 7.70997375328084 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE