FastQCFastQC Report
Wed 25 May 2016
SRR1780710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT96731.7257217847769No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT39412.926509186351707RNA PCR Primer, Index 47 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC973.18241469816273No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG802.6246719160104988No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT712.3293963254593177No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC501.6404199475065617No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT481.574803149606299No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT391.279527559055118No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGT270.8858267716535433No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT240.7874015748031495No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT240.7874015748031495No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC210.6889763779527559No Hit
ACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCT190.6233595800524935RNA PCR Primer, Index 47 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGTCTT170.5577427821522309No Hit
CTTATACACATCTCCGAGCCCACGCGACAATGGTAGATCTCGTATGCCGT140.45931758530183725No Hit
ATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTT140.45931758530183725RNA PCR Primer, Index 23 (96% over 27bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCG120.39370078740157477No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG100.32808398950131235No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCC100.32808398950131235No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGTCTT100.32808398950131235No Hit
CTTATACACATATCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT90.2952755905511811No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTAGTATGCCGT90.2952755905511811No Hit
CTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTTGA90.2952755905511811RNA PCR Primer, Index 47 (96% over 28bp)
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC80.26246719160104987No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTCTGCCGTCTT70.22965879265091863No Hit
CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT70.22965879265091863No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGTCTT70.22965879265091863No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC70.22965879265091863No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT60.19685039370078738No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC60.19685039370078738No Hit
CTTATACACATCTCCGAGCCCACGAGCCAATGGTAGATCTCGTATGCCGT60.19685039370078738No Hit
CATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCT60.19685039370078738RNA PCR Primer, Index 47 (96% over 26bp)
CTTATACACATCTCCGAGCACACGAGACAATGGTAGATCTCGTATGCCGT60.19685039370078738No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCC60.19685039370078738No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCTAGATCTCGTATGCCGT50.16404199475065617No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAAATCGTATGCCGT50.16404199475065617No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGTCTT50.16404199475065617No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT50.16404199475065617No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG50.16404199475065617No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATTTCGTATGCCGT50.16404199475065617No Hit
GTTATATTCTGAACTATGCTCGTTGAAGCCTAATTGTAACTGTCTCTTAT40.13123359580052493No Hit
TCCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC40.13123359580052493No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT40.13123359580052493No Hit
CGGCCTGGACGTGGCAACCAGCGGGGGCACCGGAACCGTCGCTGTCTCTT40.13123359580052493No Hit
CTGTTGGACTCCCTTTAGTATTTCTTATAGGGCAGTCTGAGAGGGGCCTG40.13123359580052493No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT40.13123359580052493No Hit
GTATAAGAGACAGGCAGCGTCAGATGTGTATAAGAGCTGTCTCTTATACA40.13123359580052493No Hit
CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT40.13123359580052493No Hit
ATACACATCTCCGAGCCCACGAGCCAATGGTAGATCTCGTATGCCGTCTT40.13123359580052493RNA PCR Primer, Index 47 (95% over 21bp)
TCCTGATTTACTATTGATGGGAGTTGCTATTACTTAAAAGTATACCTGTC40.13123359580052493No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACATCGTATGCCGT40.13123359580052493No Hit
TCTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT40.13123359580052493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA2000.054.2499962
CTTATAC2000.054.2499961
ATCGTAT200.006298496452.539
TATACAC2000.052.4999963
GTATGCC2400.046.66666442
TATGCCG2400.046.66666443
ATGCCGT2400.046.66666444
GACAATG2200.046.1363626
AGACAAT2200.046.1363625
TGCCGTC2450.045.71428745
CCGTCTT2450.045.71428747
GCCGTCT2450.045.71428746
TCTCGTA1850.045.40540738
CGTATGC2400.045.20833241
CAATGGT2250.045.1111128
AATGGTA2250.045.1111129
ACAATGG2250.045.1111127
GGTAGAT1950.044.87179632
CTGCTTG2500.044.854
CGTCTTC2500.044.848
Produced by FastQC (version 0.11.5)