##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780710_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3048 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55249343832021 34.0 34.0 34.0 31.0 34.0 2 32.893372703412076 34.0 34.0 34.0 31.0 34.0 3 33.34251968503937 34.0 34.0 34.0 33.0 34.0 4 33.37729658792651 34.0 34.0 34.0 33.0 34.0 5 33.42158792650918 34.0 34.0 34.0 34.0 34.0 6 37.26673228346457 38.0 37.0 38.0 36.0 38.0 7 36.74671916010499 38.0 37.0 38.0 35.0 38.0 8 36.767716535433074 38.0 37.0 38.0 35.0 38.0 9 36.599409448818896 38.0 37.0 38.0 34.0 38.0 10-11 36.29084645669291 38.0 37.0 38.0 34.0 38.0 12-13 35.88385826771653 38.0 36.5 38.0 31.5 38.0 14-15 36.011975065616795 38.0 36.5 38.0 32.5 38.0 16-17 35.76525590551181 38.0 36.0 38.0 31.0 38.0 18-19 35.751148293963254 38.0 36.0 38.0 31.0 38.0 20-21 35.78838582677165 38.0 36.0 38.0 31.0 38.0 22-23 35.94209317585302 38.0 36.5 38.0 32.5 38.0 24-25 34.680938320209975 37.0 34.5 38.0 28.0 38.0 26-27 34.73228346456693 37.0 34.0 38.0 27.0 38.0 28-29 34.45882545931759 37.0 34.5 38.0 25.0 38.0 30-31 34.0242782152231 37.0 34.0 38.0 24.5 38.0 32-33 34.75442913385827 37.0 34.5 38.0 27.0 38.0 34-35 34.055774278215225 37.0 34.5 38.0 24.0 38.0 36-37 33.8889435695538 37.0 34.0 38.0 24.0 38.0 38-39 29.693897637795274 34.0 25.0 37.0 11.0 38.0 40-41 32.31167979002625 36.0 30.5 37.0 17.5 38.0 42-43 34.60941601049869 37.0 34.5 38.0 28.5 38.0 44-45 35.08612204724409 37.0 34.5 38.0 29.0 38.0 46-47 35.05528215223097 37.0 35.0 38.0 27.5 38.0 48-49 34.851706036745405 37.0 35.0 38.0 28.5 38.0 50-51 34.712926509186346 37.0 34.5 38.0 27.5 38.0 52-53 34.08169291338582 37.0 34.0 38.0 25.0 38.0 54-55 34.746062992125985 37.0 34.5 38.0 27.0 38.0 56-57 34.79658792650919 37.0 35.5 38.0 27.0 38.0 58-59 34.240485564304464 37.0 34.0 38.0 25.0 38.0 60-61 34.04937664041995 37.0 34.0 38.0 25.0 38.0 62-63 34.74425853018373 37.0 34.5 38.0 27.0 38.0 64-65 34.82283464566929 37.0 35.5 38.0 29.0 38.0 66-67 32.7134186351706 36.5 31.0 38.0 21.0 38.0 68-69 29.295931758530184 34.0 22.5 37.0 11.0 38.0 70-71 25.626148293963254 28.0 16.5 35.5 10.5 37.5 72-73 21.41699475065617 22.0 11.0 29.0 10.5 36.5 74-75 20.94242125984252 21.0 11.0 28.5 11.0 36.0 76 21.198818897637796 21.0 11.0 28.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6771910107436341 1101 2 0.9416589988958322 1101 3 0.6397594364699657 1101 4 0.3846844675791985 1101 5 0.7452587617061326 1101 6 -1.6365514602356797 1101 7 0.9829456362351223 1101 8 0.762542282279135 1101 9 1.849601421969851 1101 10-11 1.3134334598150303 1101 12-13 1.3396603031471486 1101 14-15 1.6453022489206859 1101 16-17 2.04027146099515 1101 18-19 0.1802028380975642 1101 20-21 1.7239082189740174 1101 22-23 1.2662861261545402 1101 24-25 -0.026636849992115685 1101 26-27 0.15279457384721695 1101 28-29 2.581120816318176 1101 30-31 3.785120435120426 1101 32-33 2.818345579858736 1101 34-35 2.201491563991567 1101 36-37 1.7708602800708064 1101 38-39 -0.24169788544788418 1101 40-41 -0.29734567333251505 1101 42-43 2.0444113269771123 1101 44-45 1.020564742275269 1101 46-47 2.2007067128777678 1101 48-49 -0.022298388745753073 1101 50-51 0.5133102277839114 1101 52-53 1.5827267761478296 1101 54-55 1.3933807055517562 1101 56-57 2.8239518157281296 1101 58-59 1.5630574834522193 1101 60-61 1.2912451583504208 1101 62-63 0.09539470032891018 1101 64-65 2.0219804683620453 1101 66-67 -2.1515336637047184 1101 68-69 -1.4578270413796695 1101 70-71 -0.9342553280053281 1101 72-73 -5.6143810502363145 1101 74-75 -1.6828484015983989 1101 76 -1.0313219675061767 1102 1 0.7883021218547483 1102 2 1.0527701100069464 1102 3 0.6397594364699657 1102 4 0.7180178009125342 1102 5 0.7452587617061326 1102 6 1.0301152064309846 1102 7 -0.2392765859870991 1102 8 0.31809783783469214 1102 9 -1.150398578030149 1102 10-11 0.31343345981503035 1102 12-13 1.6729936364804843 1102 14-15 1.200857804476243 1102 16-17 1.2069381276618216 1102 18-19 1.4579806158753428 1102 20-21 1.8905748856406817 1102 22-23 1.3218416817100973 1102 24-25 -8.137747961103226 1102 26-27 0.2083501294027741 1102 28-29 1.8588985940959546 1102 30-31 3.396231546231533 1102 32-33 2.818345579858729 1102 34-35 1.64593600843601 1102 36-37 -2.6735841643736364 1102 38-39 1.2027465589965587 1102 40-41 2.0915432155563707 1102 42-43 1.4888557714215622 1102 44-45 -0.14610192439140235 1102 46-47 0.7562622684333249 1102 48-49 -0.022298388745753073 1102 50-51 1.1244213388950186 1102 52-53 1.1382823317033868 1102 54-55 3.0044918166628705 1102 56-57 2.7683962601725796 1102 58-59 2.340835261230005 1102 60-61 -5.319865952760686 1102 62-63 -0.23793863300442553 1102 64-65 -0.5335750871935048 1102 66-67 2.070688558517503 1102 68-69 1.3755062919536627 1102 70-71 5.510189116439118 1102 72-73 1.7745078386525748 1102 74-75 4.872707153957155 1102 76 2.413122476938266 1103 1 1.2327465662991912 1103 2 1.0527701100069464 1103 3 0.6397594364699657 1103 4 0.7180178009125342 1103 5 0.7452587617061326 1103 6 0.9301152064309832 1103 7 1.4273900806795652 1103 8 1.1069867267235765 1103 9 1.5607125330809666 1103 10-11 1.141211237592806 1103 12-13 1.4452158587027029 1103 14-15 -0.015808862190425543 1103 16-17 0.0458270165507102 1103 18-19 1.6524250603197856 1103 20-21 1.3461304411962445 1103 22-23 1.0440639039323187 1103 24-25 -3.3544146277698914 1103 26-27 1.1916834627361084 1103 28-29 2.6922319274292903 1103 30-31 2.657342657342646 1103 32-33 3.2016789131920618 1103 34-35 2.340380452880453 1103 36-37 1.543082502293025 1103 38-39 0.3749687812187794 1103 40-41 0.9082098822230407 1103 42-43 1.2221891047548894 1103 44-45 2.6205647422752634 1103 46-47 2.4062622684333235 1103 48-49 0.8777016112542455 1103 50-51 0.9855324500061329 1103 52-53 0.21050455392560963 1103 54-55 -1.2843970722260138 1103 56-57 2.1406184823947996 1103 58-59 1.3741685945633364 1103 60-61 -4.2531992860940235 1103 62-63 0.26761692255113445 1103 64-65 2.294202690584264 1103 66-67 1.4762441140730544 1103 68-69 0.5421729586203305 1103 70-71 -1.056477550227548 1103 72-73 -1.014381050236313 1103 74-75 -3.866181734931736 1103 76 -4.920210856395066 1104 1 0.7883021218547483 1104 2 -0.2805632233263893 1104 3 -0.13801834130781288 1104 4 0.3846844675791985 1104 5 0.4119254283727969 1104 6 0.6967818730976489 1104 7 0.871834525124008 1104 8 1.2069867267235779 1104 9 0.405156977525408 1104 10-11 -1.0198998735183054 1104 12-13 0.06188252536937 1104 14-15 1.0341911378095716 1104 16-17 -1.570839650115957 1104 18-19 1.124647282542007 1104 20-21 0.39057488564068166 1104 22-23 1.155175015043433 1104 24-25 -0.08219240554767282 1104 26-27 2.59723901829166 1104 28-29 2.9144541496515117 1104 30-31 -0.4371017871017955 1104 32-33 1.5961233576365075 1104 34-35 2.4237137862137814 1104 36-37 1.7153047245152493 1104 38-39 3.758302114552116 1104 40-41 2.8693209933341564 1104 42-43 0.9888557714215622 1104 44-45 -0.03499081328028808 1104 46-47 1.4784844906555463 1104 48-49 1.2554793890320255 1104 50-51 0.34664356111724715 1104 52-53 0.5827267761478296 1104 54-55 -0.05106373889267957 1104 56-57 -0.8427148509385347 1104 58-59 -1.6591647387700021 1104 60-61 0.06902293612819932 1104 62-63 0.9287280336622459 1104 64-65 0.18864713502870956 1104 66-67 1.4040218918508387 1104 68-69 3.819950736398109 1104 70-71 0.1768557831057862 1104 72-73 -3.336603272458536 1104 74-75 -2.960626179376181 1104 76 1.079789143604934 1105 1 1.0019773355299577 1105 2 0.8220008792377129 1105 3 0.6397594364699657 1105 4 0.4103254932202276 1105 5 0.05295106939843919 1105 6 0.49165366796944454 1105 7 1.2735439268334119 1105 8 -3.177628657891809 1105 9 1.422250994619425 1105 10-11 0.8989035452851155 1105 12-13 0.6259850894719392 1105 14-15 1.764960368578798 1105 16-17 0.13428855501224035 1105 18-19 0.7870404449351724 1105 20-21 0.3307458258116256 1105 22-23 0.5056023654707857 1105 24-25 0.6186622953070255 1105 26-27 1.6186065396591829 1105 28-29 2.961462696660064 1105 30-31 2.891958041958034 1105 32-33 3.1516789131920646 1105 34-35 -0.9480810855810873 1105 36-37 3.646928656139181 1105 38-39 -1.2288773726273732 1105 40-41 -2.357174733161571 1105 42-43 -1.8316570490912625 1105 44-45 1.7513339730444955 1105 46-47 2.5254930376640914 1105 48-49 0.2853939189465535 1105 50-51 1.6201478346215197 1105 52-53 1.0912737846948346 1105 54-55 2.1156029277739847 1105 56-57 2.1444646362409543 1105 58-59 1.366476286871027 1105 60-61 3.346800713905978 1105 62-63 1.7022323071665184 1105 64-65 2.782664229045807 1105 66-67 2.62624411407306 1105 68-69 -0.5347501183027461 1105 70-71 0.7665993728493703 1105 72-73 0.11638818053291544 1105 74-75 0.9068951881451888 1105 76 -0.8432877794719893 1106 1 0.010524344076969783 1106 2 1.0527701100069464 1106 3 -0.4713516746411486 1106 4 -0.9486488657541301 1106 5 0.7452587617061326 1106 6 0.5856707619865418 1106 7 0.4273900806795652 1106 8 0.6514311711680207 1106 9 -1.9281763558079206 1106 10-11 -0.7976776512960768 1106 12-13 2.117438080924927 1106 14-15 -2.0213644177459855 1106 16-17 1.2069381276618216 1106 18-19 0.1802028380975642 1106 20-21 -0.2205362254704255 1106 22-23 0.9885083483767616 1106 24-25 -1.0266368499921157 1106 26-27 1.4861279071805455 1106 28-29 0.7477874829848474 1106 30-31 1.6740093240093188 1106 32-33 0.3183455798587289 1106 34-35 -1.9651751026751008 1106 36-37 2.993082502293028 1106 38-39 3.1471910034410016 1106 40-41 2.147098771111928 1106 42-43 2.1555224380882194 1106 44-45 1.5761202978308262 1106 46-47 2.5340400462111035 1106 48-49 2.477701611254247 1106 50-51 -0.5978008833272028 1106 52-53 1.8605045539256082 1106 54-55 1.3933807055517562 1106 56-57 0.8239518157281296 1106 58-59 3.007501927896662 1106 60-61 2.7356896027948636 1106 62-63 2.09539470032891 1106 64-65 0.13309157947315242 1106 66-67 1.1262441140730601 1106 68-69 1.1532840697314413 1106 70-71 2.8990780053280076 1106 72-73 3.0522856164303533 1106 74-75 3.5393738206238226 1106 76 -0.3646553008395088 1107 1 0.9827465662991912 1107 2 0.3861034433402821 1107 3 -1.5269072301967022 1107 4 0.5513511342458699 1107 5 0.7452587617061326 1107 6 -0.13655146023567966 1107 7 0.5940567473462295 1107 8 -1.2096799399430864 1107 9 0.04404586641430086 1107 10-11 -0.7837887624071911 1107 12-13 1.3535491920360343 1107 14-15 -0.799142195523757 1107 16-17 -0.6541729834492926 1107 18-19 1.31909172698645 1107 20-21 -0.3455362254704255 1107 22-23 -0.20593609606768126 1107 24-25 0.23725203889677005 1107 26-27 -1.0833165372638973 1107 28-29 -0.974434739237374 1107 30-31 -0.7009906759906812 1107 32-33 0.23501224652540031 1107 34-35 -0.8262862137862186 1107 36-37 1.6180825022930279 1107 38-39 -0.8250312187812199 1107 40-41 -0.32512345111029006 1107 42-43 -0.05281089524510918 1107 44-45 0.10389807560859765 1107 46-47 -0.4937377315666751 1107 48-49 1.227701611254247 1107 50-51 1.2771991166728043 1107 52-53 0.5688378872589368 1107 54-55 -0.38439707222601527 1107 56-57 1.365618482394801 1107 58-59 -0.45083140543666644 1107 60-61 0.2634673805726493 1107 62-63 2.0676169225511316 1107 64-65 1.7442026905842667 1107 66-67 0.9179107807397244 1107 68-69 -0.9578270413796695 1107 70-71 -2.2814775502275495 1107 72-73 -1.7810477169029788 1107 74-75 -0.5578484015983989 1107 76 4.913122476938266 1108 1 0.5963829299355581 1108 2 0.780042837279673 1108 3 -0.2693314726209408 1108 4 0.7180178009125342 1108 5 -1.6183776019302307 1108 6 -1.8789757026599219 1108 7 -2.208973555684068 1108 8 -2.338467818730969 1108 9 0.05162162399005865 1108 10-11 1.0457566921382622 1108 12-13 2.0770340405208927 1108 14-15 1.4432820469004781 1108 16-17 -0.5405366198129329 1108 18-19 0.7660614239561454 1108 20-21 1.6734031684689654 1108 22-23 0.7258820857505057 1108 24-25 2.5592217358664655 1108 26-27 -0.5946801736275376 1108 28-29 -0.6714044362070695 1108 30-31 -1.265384615384626 1108 32-33 1.924406185919338 1108 34-35 0.6812895437895463 1108 36-37 1.629446138656668 1108 38-39 3.42496878121878 1108 40-41 0.3491189731321356 1108 42-43 -0.2687199861542027 1108 44-45 2.384201105911629 1108 46-47 0.9380804502515048 1108 48-49 -0.38593475238211283 1108 50-51 1.5082597227334062 1108 52-53 -1.594040900619845 1108 54-55 -0.8843970722260153 1108 56-57 0.12698211875843413 1108 58-59 -1.053104132709393 1108 60-61 0.483164350269611 1108 62-63 2.590344195278405 1108 64-65 0.28965723603881344 1108 66-67 2.0353350231639666 1108 68-69 -0.6396452231978529 1108 70-71 -3.565568459318456 1108 72-73 -0.4325628684181346 1108 74-75 -0.788909007659008 1108 76 -0.5565744927587026 1109 1 -4.467253433700808 1109 2 0.6527701100069478 1109 3 0.6397594364699657 1109 4 0.7180178009125342 1109 5 0.6452587617061312 1109 6 0.3301152064309818 1109 7 1.0273900806795666 1109 8 0.6069867267235765 1109 9 1.8607125330809637 1109 10-11 1.2412112375928075 1109 12-13 -0.7047841412972886 1109 14-15 0.1841911378095773 1109 16-17 0.6458270165507045 1109 18-19 0.8024250603197842 1109 20-21 1.746130441196236 1109 22-23 1.844063903932316 1109 24-25 0.6955853722301057 1109 26-27 0.5416834627361027 1109 28-29 -0.20776807257070828 1109 30-31 0.9073426573426531 1109 32-33 1.1516789131920646 1109 34-35 1.2403804528804514 1109 36-37 3.5930825022930293 1109 38-39 3.374968781218783 1109 40-41 4.958209882223045 1109 42-43 1.372189104754888 1109 44-45 0.9705647422752719 1109 46-47 2.1562622684333306 1109 48-49 2.027701611254244 1109 50-51 1.4355324500061357 1109 52-53 0.2605045539256068 1109 54-55 0.2656029277739904 1109 56-57 -0.8093815176051962 1109 58-59 -0.07583140543666644 1109 60-61 1.0968007139059779 1109 62-63 1.8176169225511316 1109 64-65 0.4942026905842667 1109 66-67 2.576244114073063 1109 68-69 3.24217295862033 1109 70-71 -0.5064775502275509 1109 72-73 2.785618949763684 1109 74-75 -0.866181734931736 1109 76 -2.6202108563950652 1110 1 -0.2672534337008088 1110 2 0.8527701100069436 1110 3 0.6397594364699657 1110 4 0.7180178009125342 1110 5 0.7452587617061326 1110 6 0.6301152064309861 1110 7 0.5273900806795666 1110 8 1.006986726723575 1110 9 1.7607125330809623 1110 10-11 -1.2587887624071854 1110 12-13 0.5952158587027085 1110 14-15 0.7841911378095716 1110 16-17 0.5458270165507102 1110 18-19 -1.0475749396802172 1110 20-21 1.7961304411962402 1110 22-23 -0.8059360960676827 1110 24-25 -1.2044146277698928 1110 26-27 2.0916834627361 1110 28-29 1.6922319274292903 1110 30-31 2.8073426573426445 1110 32-33 2.1516789131920646 1110 34-35 2.7403804528804514 1110 36-37 2.793082502293025 1110 38-39 2.2749687812187815 1110 40-41 -1.2917901177769586 1110 42-43 1.5721891047548908 1110 44-45 0.27056474227526905 1110 46-47 1.006262268433325 1110 48-49 1.027701611254244 1110 50-51 -1.2644675499938671 1110 52-53 0.810504553925611 1110 54-55 -0.6343970722260153 1110 56-57 -1.009381517605199 1110 58-59 -1.6258314054366636 1110 60-61 0.896800713905975 1110 62-63 1.4676169225511302 1110 64-65 1.2442026905842667 1110 66-67 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2117 5 -1.4547412382938667 2117 6 0.4301152064309832 2117 7 1.0273900806795666 2117 8 1.006986726723575 2117 9 -1.0392874669190348 2117 10-11 0.5912112375928089 2117 12-13 -3.304784141297297 2117 14-15 0.13419113780957304 2117 16-17 -0.40417298344929264 2117 18-19 -8.797574939680217 2117 20-21 -0.15386955880376263 2117 22-23 1.644063903932313 2117 24-25 -0.40441462776988857 2117 26-27 -5.858316537263896 2117 28-29 2.2922319274292917 2117 30-31 2.407342657342646 2117 32-33 -2.1483210868079325 2117 34-35 1.89038045288045 2117 36-37 -3.006917497706972 2117 38-39 -6.275031218781223 2117 40-41 1.258209882223042 2117 42-43 -3.2778108952451106 2117 44-45 -0.3794352577247295 2117 46-47 -0.6437377315666737 2117 48-49 -3.3222983887457502 2117 50-51 -1.3644675499938685 2117 52-53 -4.639495446074392 2117 54-55 -4.284397072226014 2117 56-57 1.5906184823948024 2117 58-59 -4.025831405436666 2117 60-61 -3.0531992860940207 2117 62-63 -0.6823830774488684 2117 64-65 1.4442026905842695 2117 66-67 -2.7737558859269384 2117 68-69 -1.1578270413796723 2117 70-71 -2.95647755022755 2117 72-73 -2.6143810502363145 2117 74-75 -1.0161817349317346 2117 76 -3.920210856395066 2118 1 0.6327465662991898 2118 2 -0.9472298899930536 2118 3 -5.160240563530035 2118 4 -2.281982199087466 2118 5 -0.05474123829386457 2118 6 -1.9698847935690154 2118 7 0.02739008067956661 2118 8 1.006986726723575 2118 9 -0.23928746691903768 2118 10-11 0.8912112375928061 2118 12-13 -0.8047841412972971 2118 14-15 1.634191137809573 2118 16-17 -6.904172983449293 2118 18-19 0.8024250603197842 2118 20-21 -0.5538695588037612 2118 22-23 -1.655936096067684 2118 24-25 -0.20441462776989994 2118 26-27 -5.158316537263897 2118 28-29 -4.707768072570708 2118 30-31 -6.292657342657353 2118 32-33 -0.9483210868079368 2118 34-35 -1.9096195471195472 2118 36-37 -10.40691749770697 2118 38-39 -3.7750312187812227 2118 40-41 -1.6417901177769565 2118 42-43 -2.177810895245109 2118 44-45 -6.67943525772473 2118 46-47 -3.443737731566678 2118 48-49 -1.4222983887457517 2118 50-51 -3.264467549993867 2118 52-53 -2.839495446074391 2118 54-55 -1.1843970722260124 2118 56-57 -2.009381517605199 2118 58-59 -5.625831405436667 2118 60-61 -5.153199286094022 2118 62-63 -6.182383077448868 2118 64-65 -1.0557973094157305 2118 66-67 1.6262441140730601 2118 68-69 -7.557827041379671 2118 70-71 -1.556477550227548 2118 72-73 -4.914381050236312 2118 74-75 5.883818265068264 2118 76 -5.920210856395066 2119 1 1.2327465662991912 2119 2 1.0527701100069464 2119 3 0.6397594364699657 2119 4 0.7180178009125342 2119 5 0.7452587617061326 2119 6 1.0301152064309846 2119 7 1.4273900806795652 2119 8 1.2069867267235779 2119 9 1.9607125330809652 2119 10-11 0.3412112375928089 2119 12-13 -1.6047841412972943 2119 14-15 -5.715808862190428 2119 16-17 -0.15417298344929264 2119 18-19 2.1524250603197856 2119 20-21 1.1961304411962388 2119 22-23 0.5440639039323187 2119 24-25 2.6955853722301057 2119 26-27 3.2916834627361027 2119 28-29 3.4422319274292903 2119 30-31 3.0073426573426474 2119 32-33 -4.348321086807935 2119 34-35 -1.1596195471195472 2119 36-37 0.7430825022930279 2119 38-39 1.1749687812187801 2119 40-41 2.008209882223042 2119 42-43 2.072189104754891 2119 44-45 3.020564742275269 2119 46-47 2.006262268433325 2119 48-49 2.727701611254247 2119 50-51 3.235532450006133 2119 52-53 2.360504553925608 2119 54-55 0.36560292777398473 2119 56-57 -0.259381517605199 2119 58-59 0.42416859456333356 2119 60-61 3.096800713905978 2119 62-63 -5.932383077448868 2119 64-65 -0.7557973094157333 2119 66-67 -3.12375588592694 2119 68-69 -4.7078270413796695 2119 70-71 -3.1564775502275495 2119 72-73 3.6356189497636855 2119 74-75 -3.7661817349317346 2119 76 -5.920210856395066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 3.0 24 10.0 25 8.0 26 22.0 27 47.0 28 93.0 29 121.0 30 193.0 31 260.0 32 374.0 33 506.0 34 627.0 35 500.0 36 181.0 37 101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.396325459317586 23.293963254593177 9.580052493438322 62.72965879265092 2 1.9028871391076114 4.133858267716536 84.12073490813648 9.84251968503937 3 2.65748031496063 23.62204724409449 63.681102362204726 10.039370078740157 4 1.246719160104987 54.166666666666664 20.24278215223097 24.343832020997375 5 1.9028871391076114 32.874015748031496 60.46587926509186 4.757217847769029 6 3.379265091863517 62.43438320209974 8.46456692913386 25.72178477690289 7 4.330708661417323 31.496062992125985 9.05511811023622 55.118110236220474 8 3.2480314960629917 62.13910761154856 22.244094488188974 12.368766404199475 9 2.91994750656168 12.303149606299213 4.199475065616798 80.5774278215223 10-11 3.6909448818897634 34.44881889763779 39.27165354330709 22.588582677165352 12-13 12.828083989501312 6.8569553805774275 35.84317585301837 44.47178477690289 14-15 14.74737532808399 13.353018372703412 9.366797900262467 62.53280839895014 16-17 33.41535433070867 30.905511811023622 3.1167979002624673 32.56233595800525 18-19 34.596456692913385 19.71784776902887 2.788713910761155 42.89698162729659 20-21 16.05971128608924 7.496719160104987 3.33005249343832 73.11351706036746 22-23 15.731627296587927 41.86351706036746 3.59251968503937 38.81233595800525 24-25 33.38254593175853 43.553149606299215 4.215879265091863 18.848425196850393 26-27 34.49803149606299 55.2985564304462 3.8877952755905514 6.315616797900263 28-29 16.486220472440944 37.94291338582677 13.795931758530184 31.7749343832021 30-31 15.370734908136482 36.66338582677165 39.517716535433074 8.448162729658792 32-33 66.38779527559055 17.240813648293962 12.72965879265092 3.6417322834645667 34-35 15.370734908136482 40.879265091863516 40.00984251968504 3.740157480314961 36-37 30.971128608923888 36.023622047244096 18.832020997375327 14.173228346456693 38-39 16.09251968503937 13.0249343832021 30.856299212598426 40.0262467191601 40-41 6.397637795275591 13.336614173228348 44.61942257217848 35.64632545931759 42-43 39.61614173228347 4.888451443569554 44.7014435695538 10.793963254593175 44-45 8.759842519685039 29.576771653543304 38.23818897637795 23.4251968503937 46-47 40.452755905511815 5.57742782152231 21.03018372703412 32.93963254593176 48-49 31.88976377952756 3.59251968503937 16.748687664041995 47.76902887139108 50-51 8.562992125984252 3.789370078740158 45.45603674540683 42.191601049868765 52-53 11.515748031496063 4.675196850393701 71.53871391076116 12.27034120734908 54-55 4.101049868766404 4.888451443569554 49.44225721784777 41.56824146981627 56-57 39.041994750656166 5.150918635170604 22.11286089238845 33.694225721784775 58-59 7.201443569553806 24.721128608923884 57.824803149606296 10.25262467191601 60-61 29.87204724409449 52.82152230971129 12.631233595800525 4.675196850393701 62-63 5.019685039370079 86.22047244094489 3.2480314960629917 5.511811023622047 64-65 2.083333333333333 89.94422572178478 2.3786089238845145 5.593832020997375 66-67 2.017716535433071 87.61482939632546 2.5426509186351707 7.824803149606299 68-69 2.788713910761155 81.02034120734908 4.166666666666666 12.024278215223097 70-71 3.6909448818897634 73.57283464566929 6.233595800524935 16.50262467191601 72-73 4.6587926509186355 59.104330708661415 10.9251968503937 25.311679790026247 74-75 5.46259842519685 58.00524934383202 9.744094488188976 26.78805774278215 76 4.986876640419948 60.59711286089239 8.530183727034121 25.88582677165354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 1.5 23 0.5 24 0.5 25 1.0 26 1.0 27 1.5 28 1.5 29 1.0 30 3.0 31 4.5 32 3.0 33 2.0 34 6.5 35 9.5 36 20.0 37 32.0 38 63.5 39 248.0 40 562.0 41 700.5 42 678.0 43 542.5 44 327.5 45 195.0 46 142.0 47 105.5 48 51.0 49 34.0 50 35.0 51 28.5 52 16.0 53 11.0 54 12.0 55 18.0 56 23.0 57 19.0 58 16.0 59 11.5 60 9.5 61 8.0 62 4.0 63 5.5 64 6.0 65 5.0 66 3.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 3048.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.443569553805773 #Duplication Level Percentage of deduplicated Percentage of total 1 77.54342431761788 20.50524934383202 2 11.042183622828784 5.83989501312336 3 4.962779156327544 3.937007874015748 4 1.488833746898263 1.574803149606299 5 0.7444168734491315 0.984251968503937 6 0.7444168734491315 1.1811023622047243 7 0.49627791563275436 0.9186351706036745 8 0.12406947890818859 0.26246719160104987 9 0.37220843672456577 0.8858267716535433 >10 1.8610421836228286 11.122047244094487 >50 0.37220843672456577 8.136482939632545 >100 0.12406947890818859 12.926509186351707 >500 0.12406947890818859 31.7257217847769 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 967 31.7257217847769 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 394 12.926509186351707 RNA PCR Primer, Index 47 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 97 3.18241469816273 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 80 2.6246719160104988 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT 71 2.3293963254593177 No Hit CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 50 1.6404199475065617 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT 48 1.574803149606299 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT 39 1.279527559055118 No Hit CTTATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGT 27 0.8858267716535433 No Hit CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT 24 0.7874015748031495 No Hit TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT 24 0.7874015748031495 No Hit CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 21 0.6889763779527559 No Hit ACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCT 19 0.6233595800524935 RNA PCR Primer, Index 47 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGTCTT 17 0.5577427821522309 No Hit CTTATACACATCTCCGAGCCCACGCGACAATGGTAGATCTCGTATGCCGT 14 0.45931758530183725 No Hit ATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTT 14 0.45931758530183725 RNA PCR Primer, Index 23 (96% over 27bp) TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCG 12 0.39370078740157477 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG 10 0.32808398950131235 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCC 10 0.32808398950131235 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGTCTT 10 0.32808398950131235 No Hit CTTATACACATATCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 9 0.2952755905511811 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTAGTATGCCGT 9 0.2952755905511811 No Hit CTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTTGA 9 0.2952755905511811 RNA PCR Primer, Index 47 (96% over 28bp) TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 8 0.26246719160104987 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTCTGCCGTCTT 7 0.22965879265091863 No Hit CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT 7 0.22965879265091863 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGTCTT 7 0.22965879265091863 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 7 0.22965879265091863 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT 6 0.19685039370078738 No Hit TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 6 0.19685039370078738 No Hit CTTATACACATCTCCGAGCCCACGAGCCAATGGTAGATCTCGTATGCCGT 6 0.19685039370078738 No Hit CATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCT 6 0.19685039370078738 RNA PCR Primer, Index 47 (96% over 26bp) CTTATACACATCTCCGAGCACACGAGACAATGGTAGATCTCGTATGCCGT 6 0.19685039370078738 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCC 6 0.19685039370078738 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGCTAGATCTCGTATGCCGT 5 0.16404199475065617 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAAATCGTATGCCGT 5 0.16404199475065617 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGTCTT 5 0.16404199475065617 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT 5 0.16404199475065617 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG 5 0.16404199475065617 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATTTCGTATGCCGT 5 0.16404199475065617 No Hit GTTATATTCTGAACTATGCTCGTTGAAGCCTAATTGTAACTGTCTCTTAT 4 0.13123359580052493 No Hit TCCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 4 0.13123359580052493 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT 4 0.13123359580052493 No Hit CGGCCTGGACGTGGCAACCAGCGGGGGCACCGGAACCGTCGCTGTCTCTT 4 0.13123359580052493 No Hit CTGTTGGACTCCCTTTAGTATTTCTTATAGGGCAGTCTGAGAGGGGCCTG 4 0.13123359580052493 No Hit ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 4 0.13123359580052493 No Hit GTATAAGAGACAGGCAGCGTCAGATGTGTATAAGAGCTGTCTCTTATACA 4 0.13123359580052493 No Hit CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT 4 0.13123359580052493 No Hit ATACACATCTCCGAGCCCACGAGCCAATGGTAGATCTCGTATGCCGTCTT 4 0.13123359580052493 RNA PCR Primer, Index 47 (95% over 21bp) TCCTGATTTACTATTGATGGGAGTTGCTATTACTTAAAAGTATACCTGTC 4 0.13123359580052493 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACATCGTATGCCGT 4 0.13123359580052493 No Hit TCTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 4 0.13123359580052493 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06561679790026247 0.0 2 0.0 0.0 0.0 0.06561679790026247 0.0 3 0.0 0.0 0.0 0.06561679790026247 0.0 4 0.0 0.0 0.0 0.06561679790026247 0.0 5 0.0 0.0 0.0 0.06561679790026247 0.0 6 0.0 0.0 0.0 0.06561679790026247 0.0 7 0.0 0.0 0.0 0.06561679790026247 0.0 8 0.0 0.0 0.0 0.06561679790026247 0.0 9 0.0 0.0 0.0 0.06561679790026247 0.0 10 0.0 0.0 0.0 0.13123359580052493 0.0 11 0.0 0.0 0.0 0.13123359580052493 0.0 12 0.0 0.0 0.0 0.13123359580052493 0.0 13 0.0 0.0 0.0 0.13123359580052493 0.0 14 0.0 0.0 0.0 0.1968503937007874 0.0 15 0.0 0.0 0.0 0.1968503937007874 0.0 16 0.0 0.0 0.0 0.1968503937007874 0.0 17 0.0 0.0 0.0 0.22965879265091863 0.0 18 0.0 0.0 0.0 0.32808398950131235 0.0 19 0.0 0.0 0.0 0.42650918635170604 0.0 20 0.0 0.0 0.0 0.5577427821522309 0.0 21 0.0 0.0 0.0 0.5577427821522309 0.0 22 0.0 0.0 0.0 0.5577427821522309 0.0 23 0.0 0.0 0.0 0.5905511811023622 0.0 24 0.0 0.0 0.0 0.5905511811023622 0.0 25 0.0 0.0 0.0 0.5905511811023622 0.0 26 0.0 0.0 0.0 0.5905511811023622 0.0 27 0.0 0.0 0.0 0.5905511811023622 0.0 28 0.0 0.0 0.0 0.5905511811023622 0.0 29 0.0 0.0 0.0 0.6233595800524935 0.0 30 0.0 0.0 0.0 0.6233595800524935 0.0 31 0.0 0.0 0.0 0.6233595800524935 0.0 32 0.0 0.0 0.0 0.6561679790026247 0.0 33 0.0 0.0 0.0 0.7217847769028871 0.0 34 0.0 0.0 0.0 0.8202099737532809 0.0 35 0.0 0.0 0.0 0.9186351706036745 0.0 36 0.0 0.0 0.0 1.0170603674540681 0.0 37 0.0 0.0 0.0 1.3779527559055118 0.0 38 0.0 0.0 0.0 1.4763779527559056 0.0 39 0.0 0.0 0.0 2.0341207349081363 0.0 40 0.0 0.0 0.0 2.4278215223097113 0.0 41 0.0 0.0 0.0 2.559055118110236 0.0 42 0.0 0.0 0.0 3.4448818897637796 0.0 43 0.0 0.0 0.0 3.641732283464567 0.0 44 0.0 0.0 0.0 3.8713910761154855 0.0 45 0.0 0.0 0.0 4.330708661417323 0.0 46 0.0 0.0 0.0 4.6587926509186355 0.0 47 0.0 0.0 0.0 4.757217847769029 0.0 48 0.0 0.0 0.0 5.118110236220472 0.0 49 0.0 0.0 0.0 5.282152230971128 0.0 50 0.0 0.0 0.0 5.57742782152231 0.0 51 0.0 0.0 0.0 5.905511811023622 0.0 52 0.0 0.0 0.0 5.938320209973753 0.0 53 0.0 0.0 0.0 6.036745406824147 0.0 54 0.0 0.0 0.0 6.233595800524935 0.0 55 0.0 0.0 0.0 6.725721784776903 0.0 56 0.0 0.0 0.0 6.8569553805774275 0.0 57 0.0 0.0 0.0 6.889763779527559 0.0 58 0.0 0.0 0.0 7.020997375328084 0.0 59 0.0 0.0 0.0 7.020997375328084 0.0 60 0.0 0.0 0.0 7.217847769028872 0.0 61 0.0 0.0 0.0 7.2506561679790025 0.0 62 0.0 0.0 0.0 7.480314960629921 0.0 63 0.0 0.0 0.0 7.6443569553805775 0.0 64 0.0 0.0 0.0 7.677165354330708 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACA 200 0.0 54.249996 2 CTTATAC 200 0.0 54.249996 1 ATCGTAT 20 0.0062984964 52.5 39 TATACAC 200 0.0 52.499996 3 GTATGCC 240 0.0 46.666664 42 TATGCCG 240 0.0 46.666664 43 ATGCCGT 240 0.0 46.666664 44 GACAATG 220 0.0 46.13636 26 AGACAAT 220 0.0 46.13636 25 TGCCGTC 245 0.0 45.714287 45 CCGTCTT 245 0.0 45.714287 47 GCCGTCT 245 0.0 45.714287 46 TCTCGTA 185 0.0 45.405407 38 CGTATGC 240 0.0 45.208332 41 CAATGGT 225 0.0 45.11111 28 AATGGTA 225 0.0 45.11111 29 ACAATGG 225 0.0 45.11111 27 GGTAGAT 195 0.0 44.871796 32 CTGCTTG 250 0.0 44.8 54 CGTCTTC 250 0.0 44.8 48 >>END_MODULE