FastQCFastQC Report
Wed 25 May 2016
SRR1780709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48552
Sequences flagged as poor quality0
Sequence length50
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG650.13387708024386225No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTGACGCTGCCGACG630.12975778546712802No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATC520.10710166419508979No Hit
GAAACACACTTTCTGGAGGTTTTGACATGTCTCTAAATGGCCTGTCTCTT490.10092272202998846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG207.6806324E-444.11697811
TGTGCTT207.8388624E-443.9350532
AGTCCAT207.8788213E-443.8898056
TAAACAC207.8788213E-443.8898053
CTTATGC207.8788213E-443.8898051
TTTACAC557.6397555E-1139.899822
TTACACA557.6397555E-1139.899823
CTTTACA452.3232133E-839.0131571
GCTTTTT250.002323919235.2206635
TCTTTTA250.002347618335.1480442
CTATACA451.0302629E-634.1365131
TGACGCC353.1236734E-431.51212713
TCTGCCG300.005617929.41131811
TGGCGAC300.005617929.41131816
TGCCGAG300.005617929.41131819
CTTTTAT300.005761741729.25986943
CGGTGGT602.911438E-729.25986943
TCGGTGG1151.2732926E-1124.83285542
TAGGCGG450.001399295624.3832241
TTATACA3500.023.8258932
Produced by FastQC (version 0.11.5)