FastQCFastQC Report
Wed 25 May 2016
SRR1780709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48552
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2451850.49843466798484No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT640513.19204152249135TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC26765.51161641127039No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG26615.480721700444883No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC7061.4541110561871806No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT5071.0442412259021256No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3980.8197396605701105No Hit
CATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCT2720.5602240896358543TruSeq Adapter, Index 10 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT2670.5499258526940187No Hit
ATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTT2560.5272697314219805TruSeq Adapter, Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCT2400.4943153732081068RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC1990.40986983028505525No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1530.31512605042016806No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1480.30482781347833254No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1110.22862086010874938No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG990.20390509144834404No Hit
TCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTTG930.19154720711814138TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGTCTT900.18536826495304004No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATTTCGTATGCCGT780.1606524962926347No Hit
CTTATCACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC770.15859284890426759No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG770.15859284890426759No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG610.1256384906903938No Hit
TCTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT560.11534025374855825No Hit
CACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTG550.11328060636019113TruSeq Adapter, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTA530.1091613115834569No Hit
GCCATTTAGAGACATGTCAAAACCTCCAGAAAGTGTGTTTCCTGTCTCTT490.10092272202998846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAC207.7489053E-444.0381432
TTTACAC700.044.0381432
CTTTACA750.041.1446881
TTACACA750.041.102273
CCGTCTT7950.037.3523744
ATCGTAT301.284063E-436.66065639
GCCGTCT8200.036.2135843
CTTATCA250.00230904135.2668721
TATCACA250.002320779335.2305153
TATCGTA250.002332564735.19423338
GCTAATA250.002332564735.19423333
CTAATAT250.002332564735.19423334
CTGCTTG250.002332564735.19423344
ATATCGT250.002332564735.19423337
CTTATAC33700.034.8613551
TTATACA34100.034.481482
TATACAC34700.033.9487153
CTCTTAT3900.033.345281
ATGCCGT36200.032.02237744
TGCCGTC9900.029.99508342
Produced by FastQC (version 0.11.5)