FastQCFastQC Report
Wed 25 May 2016
SRR1780708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55990
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC150.002214949369.99106629
GATCGGG150.002214949369.99106632
CGTACAC150.002214949369.99106636
GTGAGGT150.002214949369.99106624
CGCGTAC150.002214949369.99106634
GCGTCCC150.002214949369.99106619
GCCCGTC150.002214949369.99106646
GCGTACA150.002214949369.99106635
CGCCCGT150.002214949369.99106645
GTTTATC252.3728597E-455.99284739
GTGTAAA200.006679477652.966641
TTGATCG200.00692118752.49329830
GTACACA200.00692118752.49329837
TGAAGCG200.00692118752.49329829
GCGCGTA200.00692118752.49329833
TTAGCAG200.00692118752.4932984
AAGCGCG200.00692118752.49329831
CGGGGTT200.00692118752.49329835
TCAAGTA200.00692118752.49329860
CGAGGAG305.8349856E-446.66071325
Produced by FastQC (version 0.11.5)