FastQCFastQC Report
Wed 25 May 2016
SRR1780708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55990
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT8721.5574209680300053No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4400.7858546168958742Illumina PCR Primer Index 9 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1370.24468655116985177No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC950.16967315592070012No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG770.137524557956778No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT560.10001786033220217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGAA150.002213828570.09
AAATCGT200.006917693752.532
TTGGATG200.006917693752.556
TACCACT200.006917693752.54
GAATGAC200.006917693752.525
TGGGTGC200.006917693752.517
ACCTGTA200.006917693752.548
GCGCCGG200.006917693752.533
ATGCTCG200.006917693752.524
CGTCTAT200.006917693752.539
TATGCTC200.006917693752.523
AATATGC200.006917693752.521
ATGTAAG200.006917693752.51
AGCGGAG200.006917693752.510
GGGACCT200.006917693752.545
CTACGTC200.006917693752.536
TACTGTA200.006917693752.549
CGTAGCC200.006917693752.536
GTGTCTA200.006917693752.532
TCACTTG200.006917693752.527
Produced by FastQC (version 0.11.5)