##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780708_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55990 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.061260939453476 34.0 34.0 34.0 32.0 34.0 2 33.36906590462583 34.0 34.0 34.0 33.0 34.0 3 33.507679942846934 34.0 34.0 34.0 34.0 34.0 4 33.52141453831041 34.0 34.0 34.0 34.0 34.0 5 33.522128951598496 34.0 34.0 34.0 34.0 34.0 6 37.26995892123593 38.0 38.0 38.0 36.0 38.0 7 36.88251473477407 38.0 37.0 38.0 35.0 38.0 8 36.92861225218789 38.0 37.0 38.0 35.0 38.0 9 36.71589569565994 38.0 37.0 38.0 34.0 38.0 10-11 36.44141811037686 38.0 37.0 38.0 34.0 38.0 12-13 36.336042150383996 38.0 37.0 38.0 34.0 38.0 14-15 36.562296838721196 38.0 37.0 38.0 34.0 38.0 16-17 36.68720307197714 38.0 37.0 38.0 34.0 38.0 18-19 36.696338631898556 38.0 37.0 38.0 34.0 38.0 20-21 36.43044293623862 38.0 37.0 38.0 34.0 38.0 22-23 36.50778710484015 38.0 37.0 38.0 34.0 38.0 24-25 35.82982675477764 38.0 36.5 38.0 32.5 38.0 26-27 36.06453831041257 38.0 37.0 38.0 33.0 38.0 28-29 35.95367029826755 38.0 37.0 38.0 32.5 38.0 30-31 35.683970351848544 38.0 36.5 38.0 31.5 38.0 32-33 36.1794784782997 38.0 37.0 38.0 34.0 38.0 34-35 36.19646365422397 38.0 37.0 38.0 33.5 38.0 36-37 36.31582425433113 38.0 37.0 38.0 34.0 38.0 38-39 35.88669405250938 38.0 36.5 38.0 32.5 38.0 40-41 35.48450616181461 38.0 36.0 38.0 31.0 38.0 42-43 35.91765493838186 38.0 36.5 38.0 32.5 38.0 44-45 36.10565279514199 38.0 37.0 38.0 33.5 38.0 46-47 36.40589390962672 38.0 37.0 38.0 34.0 38.0 48-49 36.19210573316664 38.0 37.0 38.0 34.0 38.0 50-51 36.34949098053224 38.0 37.0 38.0 34.0 38.0 52-53 36.26252009287373 38.0 37.0 38.0 34.0 38.0 54-55 36.4376853009466 38.0 37.0 38.0 34.0 38.0 56-57 36.47994284693695 38.0 37.0 38.0 34.0 38.0 58-59 36.521289515984996 38.0 37.0 38.0 34.0 38.0 60-61 36.23142525450973 38.0 37.0 38.0 33.5 38.0 62-63 36.4811573495267 38.0 37.0 38.0 34.0 38.0 64-65 36.470137524557956 38.0 37.0 38.0 34.0 38.0 66-67 35.914958028219324 38.0 37.0 38.0 32.0 38.0 68-69 35.28892659403465 38.0 36.0 38.0 29.0 38.0 70-71 35.246704768708696 38.0 36.0 38.0 29.0 38.0 72-73 34.91510984104305 38.0 36.0 38.0 26.0 38.0 74-75 35.50103589926772 38.0 36.5 38.0 31.0 38.0 76 34.97495981425254 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34793966723338343 1101 2 0.5891065907084823 1101 3 0.12986596404364548 1101 4 0.14936081270009538 1101 5 0.19017381839809389 1101 6 -1.485852698686145 1101 7 0.3029755035727746 1101 8 0.28100063543989506 1101 9 -0.11667814525834075 1101 10-11 -0.20057258894298968 1101 12-13 0.6012773009880377 1101 14-15 0.7517509550381405 1101 16-17 0.11098781478716546 1101 18-19 -0.5306551414516392 1101 20-21 -0.11120665055882029 1101 22-23 0.6207270883011233 1101 24-25 -2.1410769988706164 1101 26-27 -0.5881923758479033 1101 28-29 0.437674189866037 1101 30-31 0.6635903151536624 1101 32-33 0.17133405106367405 1101 34-35 0.637967064981396 1101 36-37 0.6882624603884437 1101 38-39 0.5582283967835551 1101 40-41 1.0681209254280972 1101 42-43 0.8389687841413078 1101 44-45 0.4548816084596652 1101 46-47 0.6330295034514464 1101 48-49 -1.9167901434381776 1101 50-51 -1.0981487383568904 1101 52-53 -0.9898348859230595 1101 54-55 -0.6782128787088766 1101 56-57 -0.8411170447382759 1101 58-59 -1.2491964282868935 1101 60-61 -1.2389647154652437 1101 62-63 -0.10015402079842772 1101 64-65 -0.9504323152777374 1101 66-67 -1.9838034332694932 1101 68-69 -1.970411033297573 1101 70-71 -1.2247160817440417 1101 72-73 -1.6566245940343975 1101 74-75 -0.983337336248681 1101 76 -1.0625441318258098 1102 1 -0.3326488717444178 1102 2 0.2471344688705912 1102 3 0.3138102077194347 1102 4 0.2797686598864644 1102 5 0.08733437596133342 1102 6 0.37284632041555454 1102 7 -0.009311538244467954 1102 8 0.35616842067479126 1102 9 0.8940601097752179 1102 10-11 0.5269958158066217 1102 12-13 0.6547878843643886 1102 14-15 -0.38570387201399825 1102 16-17 -0.5302357267719486 1102 18-19 -0.6513572581269145 1102 20-21 0.5164391935299761 1102 22-23 0.7773868714709806 1102 24-25 -3.9329200551432066 1102 26-27 -1.0846817924715495 1102 28-29 -0.06668822624134663 1102 30-31 1.4470699227943342 1102 32-33 0.3279422080073999 1102 34-35 0.3362375864062841 1102 36-37 -3.0499151338294226 1102 38-39 -0.2119574576304828 1102 40-41 0.9354415242923153 1102 42-43 0.3951381181629827 1102 44-45 0.3461051500187793 1102 46-47 1.0022344595691521 1102 48-49 -2.6786641754464355 1102 50-51 -0.5219226154864742 1102 52-53 -0.311698592686092 1102 54-55 0.35268541762565775 1102 56-57 0.24344671365098236 1102 58-59 -0.473409128338524 1102 60-61 -5.2499352885163475 1102 62-63 -2.1034580992702914 1102 64-65 0.10005813387043361 1102 66-67 0.9241728186664844 1102 68-69 -0.516280935207746 1102 70-71 -1.1787945536077515 1102 72-73 -6.1013329058568 1102 74-75 -2.4632031080607604 1102 76 -0.4521672603751128 1103 1 0.9158883851821003 1103 2 0.5685937701956618 1103 3 0.017045451223133057 1103 4 0.34038645372573484 1103 5 0.27607125429553037 1103 6 0.010301147467700389 1103 7 0.6863088369061074 1103 8 0.4592057636450235 1103 9 0.014091085510891332 1103 10-11 -0.0313418197122175 1103 12-13 -0.9391073143965869 1103 14-15 -1.0847875065003123 1103 16-17 -0.09029423649489132 1103 18-19 0.6815243457278441 1103 20-21 -0.11441177876395159 1103 22-23 0.5284193959934385 1103 24-25 -3.191718024511644 1103 26-27 -0.316397504053036 1103 28-29 -1.04373606654422 1103 30-31 0.5982056997690464 1103 32-33 0.6988981536277805 1103 34-35 0.48668501369934347 1103 36-37 -0.8745580524320715 1103 38-39 -0.04946391090874869 1103 40-41 -0.383802151494983 1103 42-43 -0.6232107030381826 1103 44-45 1.1241123776904374 1103 46-47 0.868285913707858 1103 48-49 -2.0674311690792067 1103 50-51 -0.3084051486133035 1103 52-53 0.09926767817950832 1103 54-55 0.06729994180394527 1103 56-57 0.6902932116719853 1103 58-59 0.03029075120028324 1103 60-61 -4.069092920593448 1103 62-63 -1.367461713106124 1103 64-65 0.09379845395302766 1103 66-67 -0.04149574096180686 1103 68-69 0.4475376846511381 1103 70-71 0.5002839182559597 1103 72-73 -5.055342542752346 1103 74-75 -1.8807732336845788 1103 76 -3.047159516441198 1104 1 0.050337269630986725 1104 2 0.2942014958033923 1104 3 0.2651306993083793 1104 4 0.033276896616179386 1104 5 -0.07665934843507216 1104 6 0.01404740121395065 1104 7 0.4151633157605872 1104 8 0.39918245362171234 1104 9 0.8872179586377626 1104 10-11 -0.5166065549769527 1104 12-13 0.20187670158743032 1104 14-15 0.6353673386545253 1104 16-17 0.3066421604415126 1104 18-19 0.6405236797271812 1104 20-21 -0.13668117603334906 1104 22-23 0.3783194958935354 1104 24-25 0.7736083158146982 1104 26-27 0.9429265052709752 1104 28-29 1.2694923716842226 1104 30-31 0.4678360693994108 1104 32-33 0.5365188662484854 1104 34-35 -0.43106390404956585 1104 36-37 0.880769952895939 1104 38-39 1.3411454797006357 1104 40-41 1.892296749603915 1104 42-43 -0.16312911795660057 1104 44-45 0.7981883017663591 1104 46-47 1.1307318011537433 1104 48-49 0.014528537880501347 1104 50-51 0.05464846444031224 1104 52-53 -0.023151569239743708 1104 54-55 0.08682208632608734 1104 56-57 0.006934903313670304 1104 58-59 -0.7740715531620168 1104 60-61 0.2835128070122863 1104 62-63 -0.15579837644278882 1104 64-65 0.22679046194503627 1104 66-67 -0.34644079590685806 1104 68-69 0.8657028528163124 1104 70-71 0.9322269751990149 1104 72-73 0.34567310826330555 1104 74-75 -0.6813892843006286 1104 76 -1.3808258501075272 1105 1 0.8149166393058991 1105 2 0.45476068184028406 1105 3 0.3890036416526641 1105 4 -0.37841968705492945 1105 5 -0.07959590232704272 1105 6 0.23139374913355226 1105 7 0.6823483600156948 1105 8 -0.8489356702925903 1105 9 0.6527486063340149 1105 10-11 0.800603305226538 1105 12-13 -0.6547491566307784 1105 14-15 0.269021900653037 1105 16-17 0.24050765800127039 1105 18-19 0.6315692583523784 1105 20-21 0.6194400912344165 1105 22-23 0.3727359075074048 1105 24-25 0.8205104582582408 1105 26-27 0.5229786187625791 1105 28-29 0.657737884133553 1105 30-31 1.0267946267656143 1105 32-33 0.904332581195959 1105 34-35 -0.6189413132645569 1105 36-37 0.4094530532350902 1105 38-39 -0.04517679674902553 1105 40-41 0.5460729097495047 1105 42-43 0.620889411284864 1105 44-45 1.0244063512328196 1105 46-47 0.7427551281452196 1105 48-49 -0.032787203702746126 1105 50-51 0.8574857545387431 1105 52-53 0.4252312581239792 1105 54-55 0.7265151957644207 1105 56-57 0.4028319998477059 1105 58-59 0.3642773590722399 1105 60-61 0.8789921684152091 1105 62-63 -0.24356411389005217 1105 64-65 0.5843055583136305 1105 66-67 0.6892734898074266 1105 68-69 0.038628653130651 1105 70-71 1.0878022916023582 1105 72-73 0.6205924564310621 1105 74-75 -0.32410166745887636 1105 76 0.2279507236371927 1106 1 -0.9507602244242541 1106 2 0.2672214336120575 1106 3 0.32997430640551073 1106 4 -0.012177648838367361 1106 5 0.10534174905897942 1106 6 0.3415579188653126 1106 7 -0.41435927432537767 1106 8 0.20071894529904455 1106 9 -0.23780490582172575 1106 10-11 0.5277047675033799 1106 12-13 1.2966727506088347 1106 14-15 -0.1634711468036798 1106 16-17 0.44419474869832243 1106 18-19 0.6589981629903932 1106 20-21 0.5193458954866799 1106 22-23 0.8466750839674404 1106 24-25 -0.39156453949900083 1106 26-27 0.9621326512376882 1106 28-29 0.7426037673308201 1106 30-31 0.7211742804840995 1106 32-33 0.3640751128188242 1106 34-35 0.963210835295591 1106 36-37 1.0989883542129277 1106 38-39 0.31023273047802746 1106 40-41 0.8671458441713185 1106 42-43 0.4681128794825824 1106 44-45 0.7836465055344277 1106 46-47 0.9909384958674821 1106 48-49 1.199136183755762 1106 50-51 -0.07691363543165153 1106 52-53 0.45160606748973464 1106 54-55 0.011277912190365669 1106 56-57 0.19620689892369114 1106 58-59 0.5119628349850487 1106 60-61 0.639420983342994 1106 62-63 0.6795317863521717 1106 64-65 -1.100811513544258 1106 66-67 0.3230763067088347 1106 68-69 1.6326225528346043 1106 70-71 0.7195417730771965 1106 72-73 0.4080016248171745 1106 74-75 0.1715922412161035 1106 76 0.7366974716411434 1107 1 0.8228179535040709 1107 2 0.12084953131218867 1107 3 -0.12349333488527492 1107 4 0.14605019926290197 1107 5 -0.15860904430881817 1107 6 0.044624419132745174 1107 7 0.5328683954909863 1107 8 -0.07089809873732378 1107 9 -1.2609819427266942 1107 10-11 0.39416937795088103 1107 12-13 -0.025889203393667515 1107 14-15 -0.13797640620820317 1107 16-17 0.19370446522533058 1107 18-19 0.42718322271583276 1107 20-21 0.7142558616125498 1107 22-23 0.08611170368574506 1107 24-25 0.23448288428420483 1107 26-27 -0.35396647516630964 1107 28-29 0.4515982404989529 1107 30-31 0.1429963521546327 1107 32-33 0.7242551805670843 1107 34-35 -0.10930654748208468 1107 36-37 1.034805790476078 1107 38-39 0.4717142779909622 1107 40-41 0.48316474237064 1107 42-43 0.551773068464577 1107 44-45 0.1727978694139054 1107 46-47 0.5555222006276921 1107 48-49 1.0488865848967777 1107 50-51 0.7586185449926717 1107 52-53 0.5888408687020643 1107 54-55 0.4723226617000762 1107 56-57 0.8373639679199556 1107 58-59 0.39340337697113625 1107 60-61 1.020772382879457 1107 62-63 0.5274993385004976 1107 64-65 0.6856923195810722 1107 66-67 0.008893742971984864 1107 68-69 0.8121108751737012 1107 70-71 -0.2624960233214537 1107 72-73 0.6772118227134101 1107 74-75 -0.9042234121980499 1107 76 0.48059121384117987 1108 1 -0.2800492113579054 1108 2 0.3895699827381449 1108 3 0.022915083598789465 1108 4 0.30107536320982575 1108 5 0.30379754407000803 1108 6 -0.031172439186612166 1108 7 -0.3029559144068301 1108 8 -0.39490297901794236 1108 9 0.19898450534406464 1108 10-11 0.2828379763952853 1108 12-13 1.0003041777257593 1108 14-15 0.5717231515163661 1108 16-17 0.05945862108929134 1108 18-19 0.26929851934539073 1108 20-21 1.0009342206181913 1108 22-23 -0.3084666095672617 1108 24-25 0.7883947342155722 1108 26-27 -0.13374381236319977 1108 28-29 -0.2135306294110677 1108 30-31 0.6667877201582897 1108 32-33 0.9611394264112221 1108 34-35 0.966604692414208 1108 36-37 1.1368722842994714 1108 38-39 1.2591551808428747 1108 40-41 1.7120041506366235 1108 42-43 0.5589409806195249 1108 44-45 0.9311095973382564 1108 46-47 0.8466995683263292 1108 48-49 0.8807075396016799 1108 50-51 0.6973100197524644 1108 52-53 0.28157382584710433 1108 54-55 -0.10156783700359995 1108 56-57 0.5257504251412399 1108 58-59 0.3728146931218177 1108 60-61 0.8676154513558885 1108 62-63 0.9700035324916598 1108 64-65 0.6606427542310627 1108 66-67 1.1260204777592335 1108 68-69 1.4988197359331963 1108 70-71 -0.147885683226896 1108 72-73 0.4007896784401126 1108 74-75 -0.11887950492338462 1108 76 1.2475578144207091 1109 1 -0.7165499312184807 1109 2 0.3383083323630558 1109 3 0.31385241784141726 1109 4 0.24882864046981013 1109 5 -0.054238373183906674 1109 6 -0.5045754853334614 1109 7 -0.6273099342598272 1109 8 0.09986855996819344 1109 9 0.5160262572573799 1109 10-11 0.25070946233906 1109 12-13 0.4504064737021096 1109 14-15 0.6991384731803763 1109 16-17 0.6726472245597819 1109 18-19 0.6593303447602565 1109 20-21 0.7795770939985118 1109 22-23 0.469759502427884 1109 24-25 -0.38819843089771666 1109 26-27 0.22980472139447272 1109 28-29 -0.9689295837188752 1109 30-31 0.08792509986579944 1109 32-33 0.6785183761725264 1109 34-35 -0.36079926303021637 1109 36-37 0.5233616379404467 1109 38-39 0.837449490155592 1109 40-41 0.8581547909336606 1109 42-43 0.6093122771263069 1109 44-45 0.6432221986870488 1109 46-47 0.908380253330499 1109 48-49 0.23016196106109277 1109 50-51 -0.12516857386729896 1109 52-53 -0.10809806928057952 1109 54-55 -0.7409366329420664 1109 56-57 -0.4191044661219223 1109 58-59 -0.20047847956895026 1109 60-61 0.6387566198032673 1109 62-63 -0.25516127769247987 1109 64-65 0.3675647819646386 1109 66-67 -0.2352548120793685 1109 68-69 0.08529772335458574 1109 70-71 0.272115074521075 1109 72-73 0.4941252850173683 1109 74-75 0.16446140588968206 1109 76 0.40929911926271956 1110 1 -1.2151567300402313 1110 2 -0.93611249400427 1110 3 -0.3196958665308429 1110 4 0.13972108533884864 1110 5 0.11987196834940761 1110 6 -0.037654061879912604 1110 7 0.5771721929593383 1110 8 0.770874053161414 1110 9 0.8149674243619103 1110 10-11 -0.2102610017959563 1110 12-13 0.5627183915430507 1110 14-15 1.0139223279690128 1110 16-17 0.7569956044658426 1110 18-19 0.0062971467664709735 1110 20-21 0.9389394059163507 1110 22-23 -0.5784452583395705 1110 24-25 -0.6326057233107321 1110 26-27 0.9878056767324281 1110 28-29 0.21425646834704537 1110 30-31 0.18413559266095803 1110 32-33 0.7242551805670843 1110 34-35 0.688161806948294 1110 36-37 1.0189830056659517 1110 38-39 0.6584231387504502 1110 40-41 -0.909240320920496 1110 42-43 0.2543047140341983 1110 44-45 0.979759894730357 1110 46-47 0.511218403159333 1110 48-49 1.2102789899600666 1110 50-51 -0.13062196133644477 1110 52-53 0.6742839066767488 1110 54-55 -0.3504621484265016 1110 56-57 0.41964244893260627 1110 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38-39 -3.246105297690981 2116 40-41 -0.4215865501948812 2116 42-43 -0.4790702191089693 2116 44-45 0.0899845337034364 2116 46-47 -0.48793474356914146 2116 48-49 -0.12480747821607707 2116 50-51 -0.6966319452908039 2116 52-53 -0.02726717194688888 2116 54-55 -0.2281587075279461 2116 56-57 0.31944633554341095 2116 58-59 -0.8260653340290389 2116 60-61 -0.7725508476429184 2116 62-63 0.4999543215634361 2116 64-65 -1.5303889783329936 2116 66-67 0.0026537714975702897 2116 68-69 -0.5821661795597635 2116 70-71 0.9519361392743804 2116 72-73 0.31293120228196614 2116 74-75 -0.03263311089656895 2116 76 -0.6858377396264643 2117 1 0.6285416739223493 2117 2 0.5663641157920978 2117 3 0.34926395735467963 2117 4 -0.019424025649961152 2117 5 0.1537189688997671 2117 6 -0.017290825776449026 2117 7 0.4340791825025434 2117 8 -0.18711530658909226 2117 9 -1.4268230727945763 2117 10-11 -0.5753774941826819 2117 12-13 -1.4361808929918993 2117 14-15 -0.011348264582814238 2117 16-17 -0.6109185397278907 2117 18-19 -0.9367866910614495 2117 20-21 0.05323036170427997 2117 22-23 -0.06968539776352856 2117 24-25 -1.8479331861615833 2117 26-27 -1.3531868017118924 2117 28-29 -1.173195375351355 2117 30-31 -0.5191298632186943 2117 32-33 -1.9210851239641968 2117 34-35 0.14972849196021798 2117 36-37 -0.5058289554421052 2117 38-39 -0.10102466899125062 2117 40-41 -0.41515666654515115 2117 42-43 0.5012318833609228 2117 44-45 -0.3159656602136849 2117 46-47 -1.5248300283211336 2117 48-49 -0.9580387499041692 2117 50-51 -0.2562869769299141 2117 52-53 0.17410045409924635 2117 54-55 -0.6061950414736472 2117 56-57 -0.1005373345933549 2117 58-59 -1.4359857259457556 2117 60-61 -0.1037919172489623 2117 62-63 -0.3865531289366686 2117 64-65 -1.0886151469388352 2117 66-67 -1.4955091188882292 2117 68-69 -0.6279918582697022 2117 70-71 -1.2013604518666767 2117 72-73 -0.21253317820384865 2117 74-75 -0.5376851623356345 2117 76 -0.6229677661624891 2118 1 -0.20694598765227568 2118 2 0.13598808758997905 2118 3 -1.0787618945842112 2118 4 0.34593045926973787 2118 5 0.3957871240113988 2118 6 0.24886663603318482 2118 7 0.6320815326788036 2118 8 0.3039736084128677 2118 9 -1.7707321993123983 2118 10-11 -0.8821734205438219 2118 12-13 -0.6563630316522975 2118 14-15 0.16599211927930924 2118 16-17 -1.8570995033001623 2118 18-19 -0.5465595073560081 2118 20-21 -0.5077762971284727 2118 22-23 0.5050306226046644 2118 24-25 0.9376132298196111 2118 26-27 -2.253940816596348 2118 28-29 -0.3042177020258521 2118 30-31 -0.3498192482559048 2118 32-33 0.07710328183290471 2118 34-35 -0.056107779093444776 2118 36-37 -1.9233279762019961 2118 38-39 -0.15679239323723237 2118 40-41 -0.44747159016441884 2118 42-43 0.8119937321662505 2118 44-45 -1.894598641020579 2118 46-47 0.05184447226640998 2118 48-49 0.32775247610444325 2118 50-51 -0.7973171375252974 2118 52-53 -0.9305313516195213 2118 54-55 -0.023743024239024635 2118 56-57 0.31901809039685247 2118 58-59 -0.7838118129022789 2118 60-61 -0.6724366489371789 2118 62-63 -1.027025122669528 2118 64-65 -0.10604562089104519 2118 66-67 0.21630051683445117 2118 68-69 -0.8356397235262634 2118 70-71 0.35215502012705713 2118 72-73 1.6441550317452283 2118 74-75 0.7734194205080769 2118 76 0.7735265542448744 2119 1 0.837972169941942 2119 2 -0.43700391850061493 2119 3 0.2595517711942392 2119 4 -0.7727410291200556 2119 5 -0.6763483917860924 2119 6 -0.18661109521919172 2119 7 -0.32985223207185754 2119 8 0.4753668326229885 2119 9 0.45937819276982594 2119 10-11 0.2812258942639474 2119 12-13 -0.020228657841869335 2119 14-15 -0.17755565384592842 2119 16-17 -0.333974265386189 2119 18-19 -0.0452271891222793 2119 20-21 -1.3433301608513517 2119 22-23 0.19174550650264166 2119 24-25 1.0274495450080394 2119 26-27 0.12058335546303312 2119 28-29 0.24260376733082012 2119 30-31 1.1718785058362116 2119 32-33 0.5929483522554406 2119 34-35 0.8751826662815034 2119 36-37 -0.006645448603975979 2119 38-39 -1.4256827624797168 2119 40-41 -0.08003725442022613 2119 42-43 0.3941692175107576 2119 44-45 0.4808296041259723 2119 46-47 0.5050230029097378 2119 48-49 -0.6846666331456532 2119 50-51 0.8878750969627163 2119 52-53 0.7332962083347994 2119 54-55 0.4021229826128945 2119 56-57 -0.8601311292453175 2119 58-59 0.33590649695687347 2119 60-61 0.4246322509486333 2119 62-63 0.3978416455070999 2119 64-65 0.38862510617404666 2119 66-67 0.8160340531877068 2119 68-69 0.7875521302993889 2119 70-71 0.8779924773025414 2119 72-73 1.3094100755213987 2119 74-75 0.48497252290624715 2119 76 0.8212045138946635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 0.0 22 4.0 23 8.0 24 20.0 25 43.0 26 83.0 27 167.0 28 262.0 29 428.0 30 785.0 31 1256.0 32 1910.0 33 3023.0 34 4732.0 35 7251.0 36 12864.0 37 23152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.1778889087337 23.939989283800678 11.866404715127702 28.015717092337916 2 17.760314341846758 19.394534738346135 39.571352027147704 23.273798892659403 3 16.76013573852474 25.8778353277371 31.193070191105555 26.168958742632615 4 12.26647615645651 20.225040185747456 39.65708162171816 27.85140203607787 5 11.550276835149134 37.92820146454724 38.14252545097339 12.378996249330237 6 25.82246829791034 41.52348633684586 18.221110912662976 14.432934452580817 7 25.133059474906233 30.19289158778353 24.53295231291302 20.141096624397214 8 22.359349883907843 37.5549205215217 20.553670298267548 19.53205929630291 9 22.84336488658689 14.568672977317378 23.223789962493303 39.36417217360243 10-11 24.336488658689053 26.387747812109307 28.000535809966067 21.27522771923558 12-13 23.524736560100017 23.061260939453472 27.161100196463657 26.252902303982857 14-15 21.195749240935882 25.03661368101447 24.001607429898197 29.766029648151456 16-17 22.842471869976784 28.042507590641186 25.998392570101803 23.11662796928023 18-19 22.463832827290588 27.33613145204501 26.351134131094838 23.848901589569564 20-21 21.414538310412574 27.32630826933381 25.733166636899448 25.52598678335417 22-23 22.417395963564925 27.871941418110374 26.733345240221468 22.977317378103233 24-25 22.45222361135917 28.697981782461152 26.048401500267904 22.801393105911767 26-27 22.18074656188605 28.014824075727805 27.24057867476335 22.56385068762279 28-29 22.602250401857475 26.32791569923201 28.71852116449366 22.351312734416858 30-31 21.23146990534024 26.90212537953206 29.368637256652974 22.497767458474726 32-33 21.539560635827826 29.757099482050364 26.778889087337028 21.924450794784782 34-35 21.627969280228612 28.476513663154137 27.071798535452757 22.823718521164494 36-37 23.302375424182888 27.095909983925704 26.87890694766923 22.722807644222183 38-39 21.644043579210575 27.3450616181461 27.575459903554204 23.43543489908912 40-41 21.089480264332916 25.90909090909091 29.323093409537414 23.678335417038756 42-43 22.75852830862654 25.95016967315592 28.882836220753706 22.408465797463833 44-45 20.055367029826755 26.855688515806392 29.07394177531702 24.01500267904983 46-47 22.20843007679943 25.0500089301661 29.225754599035543 23.51580639399893 48-49 21.211823539917845 25.146454724057865 29.18556885158064 24.45615288444365 50-51 19.715127701375245 25.515270584032862 30.029469548133598 24.740132166458295 52-53 19.375781389533845 25.88140739417753 31.360064297195926 23.382746919092696 54-55 18.596177888908734 26.632434363279156 30.13663154134667 24.63475620646544 56-57 20.145561707447758 25.589390962671903 29.281121628862294 24.983925701018038 58-59 18.804250759064118 26.820860868012147 29.997320950169676 24.377567422754062 60-61 20.201821753884623 27.74334702625469 27.82729058760493 24.22754063225576 62-63 17.970173245222362 29.73656010001786 27.327201285943918 24.96606536881586 64-65 19.115020539382034 30.404536524379356 25.47419181996785 25.00625111627076 66-67 18.689944632970175 29.786569030183962 25.588497946061793 25.93498839078407 68-69 18.671191284157885 30.126808358635472 25.617074477585284 25.584925879621363 70-71 19.868726558313984 29.250759064118593 25.108948026433293 25.77156635113413 72-73 19.886586890516163 28.303268440793 24.92320057153063 26.88694409716021 74-75 20.009823182711198 28.2630826933381 25.592963029112344 26.134131094838363 76 19.657081621718163 28.937310233970354 25.874263261296658 25.53134488301482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 2.0 18 2.0 19 5.5 20 8.0 21 7.0 22 8.0 23 20.0 24 39.0 25 47.0 26 82.5 27 160.0 28 257.0 29 312.0 30 312.0 31 432.5 32 694.5 33 836.0 34 951.5 35 1217.5 36 1516.0 37 1664.0 38 1872.0 39 2606.5 40 3539.5 41 4059.0 42 4172.0 43 4180.0 44 4320.5 45 4506.0 46 4559.0 47 4514.5 48 4213.0 49 3713.5 50 3471.0 51 3197.0 52 2559.5 53 1958.5 54 1721.0 55 1640.0 56 1283.0 57 847.5 58 688.0 59 546.0 60 326.5 61 192.0 62 135.0 63 103.5 64 53.0 65 25.0 66 14.5 67 13.0 68 8.0 69 2.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.076442221825324 #Duplication Level Percentage of deduplicated Percentage of total 1 56.01106993761433 21.327022682621894 2 15.404099629438528 11.73066619039114 3 9.118626577231577 10.41614574031077 4 5.783573338336695 8.808715842114664 5 3.644636239973732 6.938739060546527 6 2.471973357099301 5.6474370423289875 7 1.6604906421501946 4.425790319699947 8 1.1492096252169426 3.500625111627077 9 0.9803461700830246 3.3595284872298627 >10 3.7431399221351844 20.757278085372388 >50 0.0187626061259909 0.500089301661011 >100 0.00938130306299545 1.030541168065726 >500 0.004690651531497725 1.5574209680300053 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 872 1.5574209680300053 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 440 0.7858546168958742 Illumina PCR Primer Index 9 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 137 0.24468655116985177 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 95 0.16967315592070012 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 77 0.137524557956778 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT 56 0.10001786033220217 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003572066440435792 0.0 2 0.0 0.0 0.0 0.005358099660653688 0.0 3 0.0 0.0 0.0 0.012502232541525273 0.0 4 0.0 0.0 0.0 0.016074298981961065 0.0 5 0.0 0.0 0.0 0.025004465083050546 0.0 6 0.0 0.0 0.0 0.025004465083050546 0.0 7 0.0 0.0 0.0 0.025004465083050546 0.0 8 0.0 0.0 0.0 0.025004465083050546 0.0 9 0.0 0.0 0.0 0.03929273084479371 0.0 10 0.0 0.0 0.0 0.0464368637256653 0.0 11 0.0 0.0 0.0 0.05179496338631898 0.0 12 0.0 0.0 0.0 0.05715306304697267 0.0 13 0.0 0.0 0.0 0.060725129487408464 0.0 14 0.0 0.0 0.0 0.060725129487408464 0.0 15 0.0 0.0 0.0 0.060725129487408464 0.0 16 0.0 0.0 0.0 0.060725129487408464 0.0 17 0.0 0.0 0.0 0.06429719592784426 0.0 18 0.0 0.0 0.0 0.07144132880871584 0.0 19 0.0 0.0 0.0 0.08394356135024111 0.0 20 0.0 0.0 0.0 0.085729594570459 0.0 21 0.0 0.0 0.0 0.08751562779067691 0.0 22 0.0 0.0 0.0 0.0928737274513306 0.0 23 0.0 0.0 0.0 0.09823182711198428 0.0 24 0.0 0.0 0.0 0.09823182711198428 0.0 25 0.0 0.0 0.0 0.10716199321307376 0.0 26 0.0 0.0 0.0 0.11609215931416324 0.0 27 0.0 0.0 0.0 0.13038042507590641 0.0 28 0.0 0.0 0.0 0.15538489015895696 0.0 29 0.0 0.0 0.0 0.1803893552420075 0.0 30 0.0 0.0 0.0 0.20003572066440437 0.0 31 0.0 0.0 0.0 0.26611894981246653 0.0 32 0.0 0.0 0.0 0.29826754777638864 0.0 33 0.0 0.0 0.0 0.34649044472227186 0.0 34 0.0 0.0 0.0 0.38042507590641184 0.0 35 0.0 0.0 0.0 0.4572245043757814 0.0 36 0.0 0.0 0.0 0.5858188962314699 0.0 37 0.0 0.0 0.0 0.860868012145026 0.0 38 0.0 0.0 0.0 1.3502411144847295 0.0 39 0.0 0.0 0.0 1.855688515806394 0.0 40 0.0 0.0 0.0 2.5343811394891946 0.0 41 0.0 0.0 0.0 3.3631005536702983 0.0 42 0.0 0.0 0.0 4.16860153598857 0.0 43 0.0 0.0 0.0 4.91516342203965 0.0 44 0.0 0.0 0.0 5.842114663332738 0.0 45 0.0 0.0 0.0 6.597606715484908 0.0 46 0.0 0.0 0.0 7.365601000178604 0.0 47 0.0 0.0 0.0 8.099660653688158 0.0 48 0.0 0.0 0.0 8.874799071262725 0.0 49 0.0 0.0 0.0 9.741025183068405 0.0 50 0.0 0.0 0.0 10.460796570816218 0.0 51 0.0 0.0 0.0 11.25558135381318 0.0 52 0.0 0.0 0.0 11.962850509019468 0.0 53 0.0 0.0 0.0 12.718342561171637 0.0 54 0.0 0.0 0.0 13.550634041793177 0.0 55 0.0 0.0 0.0 14.197178067512056 0.0 56 0.0 0.0 0.0 14.80442936238614 0.0 57 0.0 0.0 0.0 15.404536524379353 0.0 58 0.0 0.0 0.0 15.943918556885158 0.0 59 0.0 0.0 0.0 16.606536881586 0.0 60 0.0 0.0 0.0 17.179853545275943 0.0 61 0.0 0.0 0.0 17.797821039471334 0.0 62 0.0 0.0 0.0 18.333631005536702 0.0 63 0.0 0.0 0.0 18.92302196820861 0.0 64 0.0 0.0 0.0 19.426683336310056 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTGAA 15 0.0022138285 70.0 9 AAATCGT 20 0.0069176937 52.5 32 TTGGATG 20 0.0069176937 52.5 56 TACCACT 20 0.0069176937 52.5 4 GAATGAC 20 0.0069176937 52.5 25 TGGGTGC 20 0.0069176937 52.5 17 ACCTGTA 20 0.0069176937 52.5 48 GCGCCGG 20 0.0069176937 52.5 33 ATGCTCG 20 0.0069176937 52.5 24 CGTCTAT 20 0.0069176937 52.5 39 TATGCTC 20 0.0069176937 52.5 23 AATATGC 20 0.0069176937 52.5 21 ATGTAAG 20 0.0069176937 52.5 1 AGCGGAG 20 0.0069176937 52.5 10 GGGACCT 20 0.0069176937 52.5 45 CTACGTC 20 0.0069176937 52.5 36 TACTGTA 20 0.0069176937 52.5 49 CGTAGCC 20 0.0069176937 52.5 36 GTGTCTA 20 0.0069176937 52.5 32 TCACTTG 20 0.0069176937 52.5 27 >>END_MODULE