FastQCFastQC Report
Wed 25 May 2016
SRR1780707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485403
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT4940.10177110565859708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG207.8593177E-443.99164623
TCATCGT254.4684097E-543.9508482
TGCGCCA452.3244866E-839.1521412
CGACGAT250.002326331835.27699720
TCTATCG505.970469E-835.1751829
TATTGCG506.008486E-835.1498159
TACGATA250.002367925835.1498154
TCGTTGT701.02591E-931.44530734
GGCTCTA502.3383946E-630.8323112
CGTTCGG801.2914825E-1030.25673924
TTCGGTA801.3096724E-1030.22866826
GCGACGA300.005686409329.3914219
AGCGACG300.005686409329.3914218
CAGCGAC300.005692157429.38534517
GCGAAGT300.005746993729.32776622
TTGGTAA454.089937E-529.30660633
TCTCTGG454.095693E-529.30056642
GTTAAGC300.00577893129.294533
GTTGACG300.005781841529.2915137
ACGATAG300.005781841529.2915135
Produced by FastQC (version 0.11.5)