FastQCFastQC Report
Wed 25 May 2016
SRR1780707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485403
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT117202.414488579592627No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT50461.0395485812819452RNA PCR Primer, Index 14 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC16380.33745156086797984No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG13690.282033691592347No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11740.2418608867271113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC207.8489014E-444.0035069
CGAATCG207.8489014E-444.0035065
ATCGTCG207.8568707E-443.994448
ACGATGA207.8568707E-443.9944426
TTGGTAA302.52688E-643.9944433
CGTCAAC207.8568707E-443.9944421
CCGTCTT5700.040.14354744
CGACACC301.3001666E-436.6658136
GCCGTCT6350.036.03436343
TCGCTGC250.002349303635.2064312
CGTCGCT250.002349303635.2064310
GAGCAAA250.0023516835.19917735
ACTTTCA401.8155688E-533.00602715
GTAAACA401.8181308E-532.9992336
CCCGGTC401.819413E-532.995838
CCGGTCG353.213545E-431.4310749
ATGGCTC701.033186E-931.4245997
GGTTAAC502.3580396E-630.7992837
GTATATT300.00572211529.3538131
CGCTGCG300.00573654629.33869413
Produced by FastQC (version 0.11.5)