FastQCFastQC Report
Wed 25 May 2016
SRR1780706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8021
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT308838.49894028176038No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT6708.353073182894901RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG3984.961974816107717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2803.49083655404563No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2362.9422765241241744No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1141.4212691684328638No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1111.3838673482109463No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1111.3838673482109463No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT540.6732327639945145RNA PCR Primer, Index 42 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC530.6607654905872086RNA PCR Primer, Index 32 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG370.4612891160703154No Hit
CTTATACACATCTCCGAGCCCACGCGACATCACTCGATCTCGTATGCCGT340.423887295848398No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC320.3989527490337863No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC310.38648547562648045No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGTCTT310.38648547562648045No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT270.33661638199725724No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT270.33661638199725724No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG270.33661638199725724No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC260.3241491085899514No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTT260.3241491085899514No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT250.31168183518264553No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT250.31168183518264553No Hit
CTTATACACATCTCCGAGCACACGAGACATCACTCGATCTCGTATGCCGT250.31168183518264553No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC230.2867472883680339No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT230.2867472883680339No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTT200.24934546814611644No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT190.23687819473881064No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG190.23687819473881064No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT180.2244109213315048No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT180.2244109213315048No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTAGTATGCCGT180.2244109213315048No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCCCGTATGCCGT170.211943647924199No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT170.211943647924199No Hit
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT170.211943647924199RNA PCR Primer, Index 42 (95% over 24bp)
ATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGCTT170.211943647924199RNA PCR Primer, Index 32 (96% over 29bp)
CTTATACACATATCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT160.19947637451689315No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT150.18700910110958732No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC150.18700910110958732RNA PCR Primer, Index 32 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC150.18700910110958732RNA PCR Primer, Index 32 (95% over 21bp)
CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG140.17454182770228152No Hit
CTTATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT130.1620745542949757No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCC130.1620745542949757No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT130.1620745542949757No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCACGTATGCCGT120.14960728088766986No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATTTCGTATGCCGT120.14960728088766986No Hit
ATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGTCTT120.14960728088766986Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACATCACTCGATCTCGTATGCCGT110.13714000748036406No Hit
CTTATACACATCTCCGAGCCCAAGAGACATCACTCGATCTCGTATGCCGT110.13714000748036406No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT100.12467273407305822No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGTCTT90.1122054606657524No Hit
CTTATACACATCTCCGAGCCCACGAGAAATCACTCGATCTCGTATGCCGT90.1122054606657524No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT90.1122054606657524No Hit
CTTATACACATCTCCGAGCCCACGAGACAACACTCGATCTCGTATGCCGT90.1122054606657524No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACTCGATCTCGTATGCCGT90.1122054606657524RNA PCR Primer, Index 32 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA353.1268428E-970.000011
TTACACA353.1268428E-970.000013
AAAACCT150.002144796570.0000168
TTTACAC353.1268428E-970.000012
AACACCA150.002144796570.0000170
TCGATAT401.09139364E-1070.034
GAAAAAC207.458006E-570.060
CTCGATA401.09139364E-1070.033
ATCGTAT650.064.6153939
TATCGTA550.063.6363638
CGATATC453.092282E-1062.22222535
GATATCG453.092282E-1062.22222536
ATATCGT453.092282E-1062.22222537
CTTATAC6200.053.6290321
TTATACA6250.053.1999972
TATACAC6250.052.6399963
TTCTGCC200.006703605452.552
CCTGAAA200.006703605452.557
CATCACC200.006703605452.528
TGCCTGA200.006703605452.555
Produced by FastQC (version 0.11.5)